• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.279-280
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• 2008

• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.325-326
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• 2009

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.7
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• pp.975-979
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• 2010

Biological activities of different parts (stems, leaves, roots, fruits) and solvents (water, ethanol) of seabuckthorn (Hippophae rhamnoides L.) grown in Korea were tested as follows. In the experiment of inhibiting $\alpha$-glucosidase activity, ethanol extract of Hippophae rhamnoides L. stem showed the highest inhibitory activity by 93% and the next highest was the ethanol extract of its leaf by 88.7%. In the case of these two extracts, the effect of inhibiting $\alpha$-glucosidase activity was extraordinarily great when comparing with control group, acarbose. In the experiment of inhibiting $\alpha$-amylase activity, water extract of leaf showed the highest result by 54.7%, among all extracts. Regarding anticancer effect for HT-29 cell and DU-145 cell, water extract of root showed 47.1% and 32.3% activities, respectively. The experiment on antibacterial activity showed that the ethanol extract from the leaf inhibitory activity of Clostridium butyricum, Proteus mirabilis, and Shigella flexneri which are the several food borne pathogenic strains. In future research, materials for biological activity appear isolated and purified and research should continue.

• Biomedical Science Letters
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• v.25 no.2
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• pp.131-138
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• 2019

Xerostomia due to the subjective dry mouth feeling that may occur even when the salivary gland function reduction is not objectively confirmed. The purpose of this study was to investigate whether or not dry mouth is more sensitive to pain in the oral facial area, which is the main cause of dental problems. The natural products used in this study are Boswellia serrata and seabuckthorn, Both natural substances are known as a representative antioxidant substance rich in vitamins. 4-DAMP was injected into the peritoneal cavity of the experimental animals, and 5% formalin was injected into the face to observe the change of inflammatory pain. Boswellia (15, 30 mg/kg) or seabuckthorn (150, 30 mg/kg, 5 mg/kg) after formalin infusion, As a result, pain response was significantly reduced in the drug-infused group compared to the formalin-infused group (*P<0.05). It was also found that the two drugs were more effective when administered together. Based on these results, we confirm that natural extract can be an alternative treatment modality for the control of oral facial inflammatory pain.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.7
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• pp.980-985
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• 2010

The objective of this study was to isolate and identify anti-inflammatory chemicals in Hippophae rhamnoides L. which was grown in Chuncheon, Korea. Treatment of ethanol extracts from stems, leaves, roots, and fruits to RAW 264.7 cells reduced amounts of nitrite by 56.0, 31.9, 49.1, and 18.9% respectively, compared to only lipopolysaccharide (LPS) treatment which is well-known as a inflammation-inducing agent. The stems were extracted with hexane, dichloromethane, ethyl acetate, butanol, and water and their nitrite contents in RAW 264.7 cells were measured. The dichloromethane extracts showed the highest inflammatory activity, exhibiting 80% reduction of the nitrite content at 1 mg/mL treatment. Activity-directed fractionation of dichloromethane extracts led to the identification of $\beta$-sitosterol as the anti-inflammatory chemical. 0.1 mg/mL treatment of $\beta$-sitosterol inhibited strongly the production of nitrite by 65%, compared to only LPS treatment. These results suggest that stem of H. rhamnoides L. may be useful for inflammation treatment.

• Journal of Forest and Environmental Science
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• v.37 no.2
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• pp.148-153
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• 2021

This study was carried out to establish in vitro propagation system influenced by plant growth regulators through organogenesis with three different seed sources (China, Mongolia and Russia) for conservation of genetic resources in Northeast Asia. The experiment compared two different carbon sources (commercial sugar, sucrose), which showed no significant differences in germination rate. Induced adventitious buds from leaf segments were found to be highly effective when supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin, and 5.0 mg/L IAA, in the case of Chinese origin 96.8%, Russian origin R-1: 95.6%, R-2: 85.6%, and Mongolian origin M-2: 77.8%. It was effective in BA and Kinetin with supplemented with IAA, respectively. Shooting development was also efficient in Woody Plant Media (WPM) supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin and 5.0 mg/L IAA.