• 한국수산과학회지
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• 제39권2호
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• pp.66-71
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• 2006

Fluoroquinoles have a wide range of antimicrobial properties and are effective in the treatment of bacterial diseases in fish. The use of fluoroquinoles continues to grow steadily. Fluoroquinolone antibiotics are probably the most important class used among synthetic antibiotics in human and veterinary medicines because of their broad activity spectrum and good oral absorption. This study was conducted to estimate the residue of antibiotics in four species of farmed fishes, including olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major), and sea bass (Lateolabrax japonicus), collected from fish farms located in the southern coastal area of Korea. The residues of fluoroquinolones were determined using high performance liquid chromatography (HPLC) with a fluorescence detector. Residuals of five fluoroquinolones in muscle tissue of farmed fish were analyzed. We found that enrofloxacin was the most common agent in fish muscle, and that ciprofloxacin was the next most common. The range of detected concentrations of fluoroquinolones in olive flounder muscle was 0-0.859 mg/kg in 32.6% of all samples. Enrofloxacin was commonly detected in sea bass muscle at a range of 0-0.143 mg/kg in 38.9% of all samples. Fluoroquinolones were detected in 6.9% of black rock fish muscle and in 16.6% of sea bream, although the detected concentration was below 0.01 mg/kg. The maximum detection value of enrofloxacin and ofloxacin in olive flounder at the time of shipping was 0.102 mg/kg and 0.09 mg/kg, respectively; no other antimicrobials were detected. We detected no antimicrobial substances in red sea bream.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.315-321
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• 2005

Supercritical carbon dioxide extraction was investigated as a method for removing lipids and bad flavor from tuna viscera. To find the optimum conditions, different experimental variables, such as pressure, temperature, flow rate of solvent and sample size, were evaluated for the effective removal of lipids and the undesirable smell. Ethanol was used as the entrainer, with a $3\%$ by vol $CO_2$ flow rate. By increasing the pressure at constant temperature, the efficiency of the lipid removal was improved and the protein was concentrated without denaturalization. The main fatty acids extracted from the tuna viscera were palmitic acid (16:0), heptadecanoic acid (17:1), oleic acid (18:1) and docosahexaenoic acid (22:6). The major amino acids in the tuna viscera treated by supercritical carbon dioxide were glutamic acid, leucine and lysine, and the free amino acids were L-proline, taurine and L-$\alpha$-aminoadipic acid.

• Food Science and Biotechnology
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• 제18권2호
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• pp.419-424
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• 2009

Water and 70% ethanol extracts obtained from a sea cucumber (Stichopus japonicus) body wall by heat reflux or pressurized solvent extraction showed 2,2-diphenyl-1-picrylhydrazyl ($DPPH^{\cdot}$) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation ($ABTS^{{\cdot}+}$) scavenging activities comparable to those of fruits and vegetables. The highest activities were observed for the water extract from heat reflux extraction. Crude saponins exhibited higher radical scavenging activities than the soluble matters in the extracts. However, they were responsible for only about 3 to 15% of the scavenging activities of the extracts. Total phenolic contents showed a significant correlation with $DPPH^{\cdot}$ scavenging activities, suggesting a significant contribution of phenolic constituents to the antioxidant properties of the extracts. However, total flavonoid contents showed little correlation with the radical scavenging activities. The results suggest that the water or 70% ethanol extract obtained from sea cucumber body wall by simple heat reflux extraction could provide considerable antioxidant benefits.

• 한국식생활문화학회지
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• 제21권5호
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• pp.541-549
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• 2006

The purpose of this study was to investigate the antioxidative effect and quality characteristics of cookies made with Sea tangle powder(STP). The cookies were at $30^{\circ}C$ for 45 days. The 3% STP had higher pH value compared to the other groups and control. As more STP was added, the L-value and b-value decreased, and the a-value increased for the color values. The results of sensory evaluation and acceptance test showed that the cookies with 3% STP was significantly more preferable in overall acceptability than the others. The acid value, peroxide value, and thiobarbituric acid value were lower in cookies with 3% and 6% STP than in those cookies made 9% STP and the control cookies.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.89.1-89.1
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• 2007

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• 한국식품영양과학회지
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• 제30권3호
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• pp.535-539
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• 2001

The modulating effect of feeding sea mustard (Undarina pinnatifida), a fiber-rich seaweed, during initiation and post-initiation phases of colon carcinogenesis was investigated in Sprague Dawley rats. Four groups of animals were exposed to the two weekly injections of a chemical carcinogen, azoxymethane (AOM). Animals were placed on diet containing 15% sea mustard during initiation. post-initiation or initiation+post-initiation phase of carcinogenesis for 10 weeks, and colonic aberrant crypt formation and cell proliferation were compared to those of rats fed semi-synthetic control diet. Results showed that sea mustard feeding significantly reduced the numbers of both aberrant crypts and aberrant crypt foci. Also, labeling indices and proliferation zones were significantly reduced in the colon of the rats fed sea mustard diets. These results indicate that the diet containing sea mustard may decrease the risk of colon cancer development, and a part of such effect may be mediated through both the blocking of initiation and the suppression of cell proliferation in the colonic crypts, although precise mechanisms should be further examined.

• 한국식품영양학회지
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• 제12권5호
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• pp.484-489
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• 1999

Rosting conditions for improvement of viscosity and sensory properties of sea tangle extracts were investigated. The supernatant % solid yield and crude protein yield were increased by increasing of roasting temperature and times. The highest contents of algin was obtained in roasting temperature of 175$^{\circ}C$ ash contents were increased by roasting temperature increasing. Viscosities of sea tangle extract were significantly decreased by increasing of roasting temperature and time upto 175$^{\circ}C$ and 10 mins more than further roasting conditions. The pH of sea tangle extracts slightly decreased from 5.94 to 5.83 in the roasting of 15$0^{\circ}C$ however at temperature more than 175$^{\circ}C$ its pH was increased by increasing of temperature and time. According to increase of roasting temperature and time Lightness (L value) were significantly decreased and redness (a value) and yellowness (b value) reached the highest value in the roasting of 20$0^{\circ}C$ 15 min. or 175$^{\circ}C$ 30 min and after that its value were decreased. The odor characteristics showed that sea tangle extract prepared by roasting of 175$^{\circ}C$ 10mins was slightly reduced in intensity of savory and seaweed taste but significantly low in intensity of nauseous taste and high in intensity of roasted taste and accetability. Overall data suggested 175$^{\circ}C$ 10min was the most effective roasting conditions for improvement of viscosity and sensory properties of sea tangle extract.

• 한국식품영양과학회지
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• 제27권2호
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• pp.350-358
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• 1998

The antimutagenic and anticancer activities of glycoprotein(GP) and chondroitin sulfate(CS) from sea cucumbers were studied using Ames mutagenicity test and human cancer cells culture test. The GP's inhibitory effect toward aflatoxin B1(AFB1) and 3, 2'-dimethyl-4-amino-biphenyl(DMAB) increased with the higher added concentrations up to 5% level(w/w) regardless fractionation methods. The GP from sea cucumbers through DEAE-cellulose column chromatography showed an inhibitory effect ranged from 84 to 98%, and the maximum antimutagenicities resulted in red sea cucumber with 98% (AFB1) and 95% (DMAB). But 5% level of CS from various sea cucumbers had an inhibitory effect toward those both indirect mutagens ranged from 79% to 85%. However, in case of direct mutagens(N-methyl-N'-nitro-N-nitrosoguanidine, MNNG and 4-nitroquinoline-1-oxide, 4-NQO), the GP's inhibitory effect was 55∼78% and the CS had a low inhibitory effect(58∼70%) at the added level of 5%. The GP from sea cucumbers exhibited the strong inhibitory effects with 89∼95% and 82∼92% on the growth of HT-29 human crcinoma cells and AZ-521 human gastric cancer cells (at 5% level).

• 한국식품영양과학회지
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• 제43권5호
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• pp.784-790
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• 2014

본 연구에서는 다시마음료의 유통기한을 pH, 총산도, 색도, 미생물 관능검사에 의해 예측하였다. 다시마음료의 pH와 총 산도는 저장온도에 따른 유의적인 차이를 나타내지 않았다. 색도 측정 결과 저장온도(15, 25, $37^{\circ}C$)에 따른 명도는 유의적인 변화를 보이지 않았으며, 적색도는 감소하였다. 황색도는 $15^{\circ}C$에서는 변화가 없었으며, $25^{\circ}C$, $37^{\circ}C$에서는 저장 8개월에 증가하였다. 저장 초기 시료와 차이 정도를 나타내는 ${\Delta}E$ 값은 $15^{\circ}C$에서 가장 차이(12.14)가 적었으며, 저장온도가 증가($25^{\circ}C$, 12.57; $37^{\circ}C$, 14.43)할수록 차이 정도가 증가하였다. 저장기간 동안 총균과 대장균군은 검출되지 않았으며, 관능검사 패널들은 신선한 다시마음료와 저장 음료와의 차이를 3점 이하로 나타내어 저장기간 중 관능상의 확연한 변화는 나타나지 않았다. 이들 결과를 바탕으로 다시마 음료의 우리나라 연간 온도별 예상 유통일수를 근거로 산출한 유통기한은 결정계수가 가장 높았던 색도를 기준으로 27.10개월로 예측되었다.

• 한국식품영양학회지
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• 제21권4호
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• pp.592-598
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• 2008

Deep sea water is found more than 200 m under the surface. As no sunlight reaches, no photosynthesis takes place, and it has very little organic matter or bacteria. In addition, deep sea water maintains a consistently low temperature throughout the year, and it does not mix with the water found closer to the surface, which means that its cleanliness is preserved. It is a long-term mature sea water resource that is rich in minerals. This paper examined the physical characteristics and the uses of deep sea water, a subject that has been attracting a great deal of public attention recently, together with the current status of domestic research into it and the direction of research in the USA and Japan, focusing on the existing literature. The aim of this paper was to provide are source to researchers in the field. Since the 1970s, scientists around the world have recognized the importance of deep sea water, and have been conducting research into it. In the USA, deep sea water has been researched with the view of its application to cooling, alternative energy, farming, and the development of new materials. In Japan, about 10 local self-governing bodies are currently promoting research and business relating to deep sea water, which has resulted in a number of products that have been released to the market. In Korea, the ministry of land transport and marine affairs has been studying deep sea water since 2000, and full-scale national R&D projects have been performed by 24 organizations, including KORDI, through industrial/academic cooperation. Large companies are participating in deep sea water research projects in several ways. A study of data foundusing NDSL relating to domestic studies of deep sea water found 50 theses, 177 domestic patents, 6 analyses, 2 reports, and 2 etc. in other areas.