• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.135-140
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• 2012

양식의 성공으로 생산되는 다량의 홍해삼의 이용도를 다양화하기 위해 홍해삼 김치를 개발 연구하였다. 김치 숙성 정도의 지표인 총산도와 pH는 7일째 까지 감소현상이 심하였으나, 숙성후기에는 pH의 변화가 느려지고, 적정 pH인 4-4.5에 머물렀다. 환원당 함량은 초기에 다소 높게 나타났다가 저장기간 동안 지속적인 감소를 보였다. 생홍해삼을 넣은 김치와 삶은 홍해삼을 넣은 김치의 발효과정에 따른 유산균 변화는 저장초기에 급속히 증가하였고 삶은 해삼보다는 생해삼에서 더 많은 유산균 발육을 확인 할 수 있었다. 생해삼과 삶은 해삼 그리고 젓갈이 홍해삼 김치의 기호도에 미치는 영향을 측정한 결과 해삼을 첨가한 김치는 일반 김치에 비하여 색, 향, 질감, 맛, 뒷맛, 기호도 모두 뒤떨어짐이 없었다.

• 한국양식학회지
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• 제21권1호
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• pp.19-25
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• 2008

본 연구는 어린 돌기해삼 Stichopus japonicus 사료의 적정 단백질과 지질 함량을 조사하기 위해 단백질(10%, 20%, 30% 및 40%)과 지질(3%, 7% 및 11%)이 달리 함유된 12 종류의 실험사료를 제조하였다. 평균 체중 1.1 g의 치삼을 각 수조마다 50마리씩 3반복으로 수용하여 8주간 수행하였다. 사육실험 종료 후, 생존율은 86% 이상으로 실험구간에 유의한 차이가 없었다(P>0.05). 증중율과 일간성장율은 동일한 지질함량에서 단백질 30%에 지질 3% 함유 사료 공급구가 다른 실험구에 비해 유의하게 높은 값을 나타내었다(P<0.05). 치삼의 수분, 지질 및 회분 함량은 사료의 단백질과 지질함량에 따른 차이를 보이지 않았으나, 단백질함량은 실험구간에 유의한 차이를 보였으며(P<0.05), 단백질 30%에 지질 3% 함유 사료 공급구에서 가장 높게 나타났고, 단백질 20%에 지질 11% 공급구에서 가장 낮았다. 지방산 조성은 사료의 지방산에 영향을 받아 지질함량이 증가함에 따라 18:2n-6은 증가하였고 n-3HUFA는 감소하는 경향을 보였다. 이상의 결과로부터, 해삼의 최대 성장에 필요한 단백질함량은 30%이고, 지질함량은 3%로 판단된다.

• 한국패류학회지
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• 제27권4호
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• pp.331-336
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• 2011

2011년 4월 20일부터 7월 15일까지 전복배합사료와 건다시마를 먹이원으로 한 북방전복과 해삼의 실내 복합사육에서 생존율과 성장에 어떠한 영향을 받는지 관찰하였다. 실험에 사용된 북방전복의 크기는 각장 $28.54{\pm}1.23mm$, 전중량 $2.92{\pm}0.26g$ 이었고, 해삼은 습중량 $3.0{\pm}0.2g$ 이었다. 먹이는 전복배합사료 실험구, 건다시마 실험구 및 전복배합사료와 건다시마 혼합 실험구로 설정하였다. 북방전복과 해삼의 생존율은 모든 실험구에서 각각 97.0%, 87.0% 이상을 보였다. 북방전복의 생존율은 건다시마 실험구에서 가장 높은 반면 해삼은 가장 낮았지만 유의적인 차이는 보이지 않았다. 성장은 북방전복의 경우 전복배합사료 실험구에서, 해삼의 경우 전복배합사료와 건다시마를 동시에 공급한 실험구에서 가장 빨랐다.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2005년도 아시안 푸드의 기능성과 세계화 전략에 관한 국제심포지움
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• pp.112-123
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• 2005

For thestudying and utilizing the thorn sea cucumber synthetically, the method preparing polysaccharide and polypeptide from sea cucumber was studied. The comparatively rational craft for preparing crude polysaccharide is fresh raw materials deal with oar form, $60\%$ of the ethanol precipitate after hydrolyzed by pretense and vibrated with ultrasonic wave auxiliary. The purification of polysaccharide and removal of protein fromcrude polysaccharide were made through precipitation method using the acetate. The polypeptide is obtained by concentrating in vacuum, freeze-drying after mixing precipitations of two times. Polysaccharide acute poisoning experiment indicate there is no bad reaction when LD50>5000g/kg, there strain rate of liver tumor H11 approach present generally acknowledged tumor treat medicine cyclophosphamide, and there is no side effect. The fatigue resistance function of polypeptide experiment also indicates that the fatigue resistance ability of mouse which fed on added sea cucumber polypeptide has a great improvement.

• Fisheries and Aquatic Sciences
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• 제25권5호
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• pp.264-275
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• 2022

A non-destructive environmental DNA protocol for the genetic analysis of sea cucumber (Apostichopus japonicus) resources DNA was established. Among the several commercial DNA extraction kits, the DNeasy^® Plant Mini Kit was selected as the best choice to obtain the high-quality genomic DNAs from the mucous sea cucumber. As the temperature and incubation time increased, the amount of extracted environmental DNA was also large, but it was judged that the increased amount did not affect as much as 2-3 times. Therefore, these conditions were not considered to be the main factors to consider in actual environmental DNA extraction. However, the amount of seawater relative to the size of the sample was judged as a major consideration, and a sufficient amount of environmental DNA for analysis was secured when stored within 1 min while stirring the volume of seawater corresponding to the total sea cucumber weight (g). In securing the environmental DNA of sea cucumbers, the mortality rate of sea cucumbers in all experiments was 0, and it was judged that the effects of sea cucumbers were not significant through this treatment. Through the results of this study, sea cucumber DNA research, which has been conducted in a destructive method, can be conducted non-destructively through environmental DNA analysis. Through this study, we have secured a standard protocol that can successfully extract the sea cucumber DNA through environmental DNA. It is not only excellent in terms of time and cost of traditional DNA analysis method currently used, but it is completely non-destructive in the ecosystem of the survey area. It is believed that the system can be transformed in a way that does not affect it. However, it is thought that various standard protocols should be established considering the characteristics of each type.

• 동의생리병리학회지
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• 제26권5호
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• pp.672-678
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• 2012

Colitis and constipation are the most common intestinal complaints worldwide. This study examined the beneficial effects of sea cucumber powder for TNBS-induced colitis in ICR mice(n=6/group) and loperamide-induced constipation in rats(n=8/group). Animals were divided into normal and four dried see cucumber treated groups that were named Nor, Con(0 mg/kg), GI(30 mg/kg), GII(100 mg/kg) and GIII(300 mg/kg). In order to induce colitis and constipation, 5% TNBS was injected into distal colon and loperamide (2 mg/kg, twice a day, peroral) was treated for 5 day in the four experimental groups but not the normal group. Gross finding score was decreased tendency by oral administration of sea cucumber in colitis-induced animal model, but colonic weight was not different. Excreted fecal pellet number, weight and water content were increased in the sea cucumber-treated group compared to the non-treated group. The fecal pellet number was reduced within distal colon of the sea cucumber-treated groups. Interestingly, intestinal transit length was increased compared to the non-treated group. Our results demonstrated that colitis and constipation were improved by sea cucumber treatment in the animal models. Specifically, gross finding score in TNBS-induced colitis model was improved by sea cucumber. After animals being fed sea cucumber, excreted fecal number, weight, water contents, and fecal number within distal colon and colonic transit length also get better in loperamide-induced constipation model.

• 수산해양기술연구
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• 제53권1호
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• pp.49-59
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• 2017

The objective of this study was to investigate the growth rate and the optimal stocking density of sea cucumbers. Grow-out was studied in situ by conducting a follow-up survey using visible implant elastomer (VIE) tags. The rearing systems were made of polypropylene pipe for the frames and netting. The experiment ran for 70 days near Yeosu, Korea in the water depth of about 7 m. A total of 576 sea cucumbers which have three groups of body sizes (small: 5.15, medium: 12.34 and large: 23.26 g) were used. The five groups of stocking densities (150, 300, 450, 600 and $850g/m^2$) in rearing system for sea cucumber were considered. Sea cucumbers were fed a mixed diet (mud, mineral, fish meal, etc.). The feed was supplied to 10% of their body wet weight once every 7 days. The survival rate (73%) of sea cucumber in $850g/m^2$ was lower than those of other density groups ($150g/m^2$: 89%, $300g/m^2$: 84%, $450g/m^2$: 78% and $600g/m^2$: 86%). The survival rate of medium size group was higher than those of small and large groups regardless of the density (P<0.05). Most of density groups have no significant difference except for $850g/m^2$ (P>0.05). The growth rate of small size group ($0.63%day^{-1}$) was higher than those of medium ($0.38%day^{-1}$) and large ($0.34%day^{-1}$) group regardless of the density (P<0.05). The threshold water temperature was $11.0^{\circ}C$ for sea cucumber growth in winter season.

• 한국응용과학기술학회지
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• 제39권1호
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• pp.108-114
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• 2022

본 연구에서는 천연 생리활성물질인 에코사펜타엔산을 다량 함유한 흑해삼의 항산화 효과를 조사하여 기능성 화장품 원료로서의 우수성을 제시하였다. 세포내 산화질소(NO) 생성 억제 효과에 대해 해삼 추출물의 활성산소 제거능을 평가하였다. 해삼의 생리활성물질은 활성산소를 제거하는 항산화 효과가 있으며, 평가 결과 우수한 NO 생성이 억제됨을 확인하였다. 연구에 따르면 생리활성 물질은 낮은 농도에서도 높은 항산화 효능을 보여 항산화 효과, 면역 및 염증 반응 조절에 관여하는 것으로 나타났습니다. 해삼의 항산화 제거능은 해삼 추출물의 EPA(eicosapent aenoic acid)를 함유하는 페놀 성분의 항산화 효능에 크게 기여하는 것으로 생각된다. 해삼의 항산화 성분이 작용한다는 보고와 유사한 것으로 밝혀졌다. 또한 해삼 추출물에 다량 함유된 에이코사펜타엔산(EPA) 성분이 염증 활성 억제 효과에 관여하는 것으로 입증돼 상처 치유에도 탁월하다. 본 연구의 결과, 해삼 추출물에 함유된 페놀 성분의 효과가 항산화 활성과 밀접한 인과관계가 있음을 확인하였다.

• 한국해양바이오학회지
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• 제14권2호
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• pp.61-68
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• 2022

This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.

• 현장농수산연구지
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• 제19권1호
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• pp.15-23
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• 2017

전복 방류 효과를 조사한 결과 크기가 큰 종묘(전중, 3.2 g)를 방류한 월항어촌계가 크기가 작은 종묘를 방류한 화태어촌계에 비하여 방류효과가 좋은 것으로 나타났으며, 방류 후 10개월이 경과된 후 월항어촌계의 해삼 생존율은 66%로 매우 높게 나타났다. 이러한 결과는 방류효과가 종묘의 크기에 따라 큰 차이를 보이는 것으로 나타났다. 또한 방류해역에 따른 해삼의 생산효과를 분석한 결과 어류 가두리 양식장이 밀집한 화태어촌계에서 실험종료 시 성장(6.2 g)과 생존율(32.1%)이 가장 좋은 것으로 나타났는데 이것은 오염도는 높지만 지속적으로 동물성먹이가 공급되는 해역의 생산성이 높다는 것을 의미한다. 따라서 자원조성 및 종 보존을 위해서 어류 양식가두리 저면을 활용하는 것도 고려할 방법으로 판단된다.