• Title/Summary/Keyword: scorpion

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Modification of Insect Sodium Currents by a Pyrethroid Permethrin and Positive Cooperativity with Scorpion Toxins (피레스로이드계 살충제 퍼메트린이 Heliothis virescens 중추신경세포에 있는 나트륨채널에 작용하는 기작을 전기생리학적으로 연구)

  • Lee, Daewoo;Adams, Michael E.
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.117-128
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    • 2022
  • In this study, we have examined pyrethroid actions on sodium channels in the pest insect Heliothis virescens. The synthetic pyrethroid permethrin increased steady-state sodium current in H. virescens central neurons and prolonged tail currents (INa-tail) due to extreme slowing of sodium channel deactivation. Prolongation of INa-tail was evident at permethrin concentrations as low as 60 nM, which modified ~1.7% of sodium channels and 10 μM permethrin modified about 30% of channels. The average time constant (τ1) of tail current decay was ~335 ms for permethrin-modified channels. These modified channels activated at more negative potentials and showed slower activation kinetics, and failed to inactivate. Permethrin modification of sodium channels was dramatically potentiated by the α scorpion toxin LqhαIT, showing positive cooperativity between two binding sites. The amplitude of the tail current induced by 0.3 μM permethrin was enhanced ~8-fold by LqhαIT (200 pM). Positive cooperativity was also observed between permethrin and the insect-specific scorpion toxin AaIT as 10 nM permethrin potentiated the shift of voltage dependence caused by AaIT (~2-fold).

A Study on the Development Trend of MUMT System of Military Advanced Countries (군사 선진국의 유·무인 복합체계 개발동향 연구)

  • Sang-Keun Cho;Eui-chul Shin;Jun-Woo Kim;In-Chan Kim;Ki-Won Kim;Sang-Hyuk Park
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.1
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    • pp.413-418
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    • 2023
  • This study presents the direction of the Republic of Korea Army in the future by examining the Manned and Unmanned Teaming(MUMT) Systems of the Military Advanced Countries of the United States, Israel, and France. In this study, the current status of the U.S. Ground Forces' 'Squad-X' program, the Israeli Ground Forces' Digital Army Program (DAP), and the French Army's Scorpion program were examined. Next, it was followed by a discussion of The Combat concept, a way of fighting in the future battlefield, with the development trend of a complex Combat system with various weapon systems supporting it. Finally, based on this, the direction of the development of MUMT system, which the Republic of Korea Army should engage in in the future, was presented as a conclusion. Since such MUMT system of advanced military forces are being developed in a secret manner, continuous longitudinal research needs be conducted.

A VELOCITY STRUCTURE ANALYSIS OF GIANT MOLECULAR CLOUD ASSOCIATED WITH HII REGION S152 (HII 영역 S152에 접해 있는 거대 분자운의 속도 구조 분석)

  • Choi, Woo-Yeol;Min, Y.C.;Lee, Yeong-Ung;Park, Myeong-Gu
    • Journal of Astronomy and Space Sciences
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    • v.22 no.2
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    • pp.125-138
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    • 2005
  • S152 is a small bright emission nebula located in the Perseus arm. Its optical diameter corresponds to 1.5 pc for an adopted distance 3.5 kpc. However, S152 is a part of a giant molecular cloud complex, which consists of several dense cores, containing active star-forming sites, and well aligned arm-like features. We analyzed the FCRAO $^{12}CO(J=\;1{\to}0)$ Outer Galaxy Survey data in this region to study the kinematical structure of this region, which resembles a big "scorpion". We found that there exist three different velocity components, about -54.5, -50.4, -48.8 km $s^{-1}$, depending on the position of the "scorpion". There also exist velocity gradients of 0.21 km $s^{-1}pc^{-1}$ and 0.16 km $s^{-1}pc^{-1}$ through the whole extent of the "scorpion". Interestingly, these two velocity gradients show an opposite direction with each other. It is likely that the velocity structure of this region may result from the mergence of different gas clouds, and the interaction with the SNR 109.1-1.0 occurred later, mostly at the region around the "head of the scorpion" only.

Anteroinferior Capsulolabral Complex Repair Using Antegrade Suture Passer - Technical Note - (Antegrade Suture Passer를 이용한 전하방 관절낭-관절와순 복합체의 복원술 - 수술 술기 -)

  • Seo, Hyuk-Jun;Cho, Chul-Hyun;Lee, Si-Wook
    • Journal of the Korean Arthroscopy Society
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    • v.17 no.1
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    • pp.95-99
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    • 2013
  • We introduce arthroscopic Bankart repair technique using antegrade suture passer that can effectively restore detached anteroinferior capsulolabral complex for shoulder anterior instability. After diagnostic arthroscopy is performed using posterior, anteroinferior and anterosuperior portals, we confirm Bankart lesion and perform debridement and decortications of anteroinferior glenoid edge and neck. Suture anchor is inserted through anteroinferior portal at 2 mm medial side of glenoid edge (4:30 direction). Scorpion$^{TM}$ loaded suture is directly advanced to detached and retracted anteroinferior capsulolabral complex and the suture is passed at 10~15 mm medial side of detached anteroinferior capsulolabral complex (5:30 direction). The suture is retrieved by Scorpion's hook and then is tied using samsung medical center (SMC) sliding knot technique. Then suture anchors are serially inserted (2:30, 3:30) and capsulolabral complex repair is performed using suture hook and suttle-relay technique. This technique that can obtain anatomical restoration of anteroinferior glenohumeral ligament with proper tension is useful technique to reduce postoperative recurrence and makes it possible for less experienced surgeons.

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Hypoglycemic Effects of Eryngium Creticum

  • Jaghabir, Madi
    • Archives of Pharmacal Research
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    • v.14 no.4
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    • pp.295-297
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    • 1991
  • E. creticum has been used in folk medicine in Jordan as a remedy for scorpion stings in the rural areas and as a hypoglycemic agent. The hypoglycemic activity of an aquous decoction of plant arial parts of E. creticum was tested in normoglycemic and streptozocin-hyperglycemic rats. Results indicate that those extract caused significant reductions in blood glucose concentration when given orally.

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Enzyme-linked Immunosorbent Assays (ELISA) and Immunochromatography Assays (ICG) for Analysis of Vitellogenin in the Scorpion Fish Sebastiscus marmoratus (쏨뱅이(Sebastiscus marmoratus)의 Vitellogenin 분석을 위한 효소면역측정법(ELISA) 및 면역크로마토그래피분석법(ICG) 개발)

  • Yeo, In-Kyu;Lim, Yoon-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.4
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    • pp.459-465
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    • 2015
  • We tested biomarker systems [enzyme-linked immunosorbent assay (ELISA) and immunochromatography assay (ICG) kits] for the screening of endocrine-disrupting chemicals in contaminated environments using antibodies resulting from $17{\beta}$-Estradiol-induced vitellogenin (Vtg) in the wild scorpion fish Sebastiscus marmoratus. Monoclonal antibodies of two clones (S28 and S15) were used as capture and tracer antibodies for ELISA and ICG assays. ELISA detected Vtg at levels greater than $0.1{\mu}g/mL$, while ICG detected Vtg at levels greater than $1{\mu}g/mL$. However, the ICG system was able to detect antibodies from $17{\beta}$-Estradiol-induced Vtg serum that had been diluted 1,000 times. Our results suggest that previously developed biomarker assays can be used as detection systems to detect known endocrine-disrupting chemicals in contaminated environments, and to measure their activity.

First Record of Maritime Pseudoscorpion Garypus japonicus (Garypidae) from Korea

  • Choi, Eun-Hwa;Hwang, Ui-Wook
    • Animal Systematics, Evolution and Diversity
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    • v.25 no.3
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    • pp.261-264
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    • 2009
  • A maritime pseudo scorpion Garypus japonicus Beier, 1952 collected from Dokdo Island in the East Sea of Korea is reported and illustrated, which is the first record in Korea. The family Garypidae is also newly recorded in Korea. G. japonicus has been known only from Japan by Beier (1952) so far. Through the present study, its distributional range is extended to Korea.

Expression of a Fusion Protein with Cry1Ac Protein and a Scorpion Insect Toxin in Acrystalliferous Bacillus thuringiensis Strain

  • Roh, Jong-Yul;Li, Ming-Shun;Chang, Jin-Hee;Park, Jae-Young;Shim, Hee-Jin;Shin, Sang-Chul;Boo, Kyung-Saeng;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.89-93
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    • 2004
  • Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

A Genetically Engineered Pseudomonas fluorescens Strain Possesses Dual Activity Against Phytopathogenic Fungi and Insects

  • Lu, Wenwei;Zhang, Weiqiong;Bai, Yan;Fu, Yingying;Chen, Jun;Geng, Xiaolu;Wang, Yujing;Xiao, Ming
    • Journal of Microbiology and Biotechnology
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    • v.20 no.2
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    • pp.281-286
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    • 2010
  • A Pseudomonas fluorescens strain was isolated and found to show antagonistic activity against phytopathogenic fungi and to possess a gene responsible for production of antibiotic 2,4-diacetylphloroglucinol. For the extension of biocontrol range, a gene for an Androetonus australis Hector insect toxin 1 (AaHIT1), one of the most known toxic insect-selective peptides, was designed and synthesized according to the preferred codon usage of Pseudomonas fluorescens, cloned, and transformed into the strain by pSUP106 vector, a broad-host-range plasmid. Bioassays indicated that the engineered strain was able to produce AaHIT1 with insecticidal activity, and at the same time retain the activity against plant pathogen. The experiments for nonplanted soil and rhizosphere colonization showed that, similar to the population of the wild-type strain, that of the engineered strain remained relatively constant in the first 10 days, and the subsequent 50 days, suggesting that AaHIT1 expression in the bacterial cell does not substantially impair its long-term colonization. It is first reported that a Pseudomonas fluorescens strain expressing an active scorpion neurotoxin has dual activity against phytopathogenic fungi and insects, making at attractive for agronomic applications.