• Archives of Pharmacal Research
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• v.28 no.2
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• pp.203-208
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• 2005

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin $E_2\;(PGE_2)$ upon stimulation by IFN-${\gamma}$/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-${\gamma}$/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and $PGE_2$ in macrophages by inhibiting iNOS and COX-2 expression.

• Korean Journal of Pharmacognosy
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• v.49 no.1
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• pp.23-27
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• 2018

Five compounds were isolated from the water extract of Oplopanax elatus stem. On the basis of spectral data, the structure of isolated compounds were identified as scoparone (1), uracil (2), protocatechuic acid (3), syringin (4), and adenosine (5). Among the these compounds, scoparone (1), uracil (2), protocatechuic acid (3), and adenosine (5) were isolated for the first time from this plant.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.580-581
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• 2006

• Natural Product Sciences
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• v.16 no.4
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• pp.233-238
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• 2010

In order to facilitate the quality control of Artemisia capillaris, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the six bioactive compounds: scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-Orobinobioside (5), and scoparone (6), which were selected as the chemical markers of A. capillaris. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity ($R^2$ > 0.998). A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds scopolin, chlorogenic acid, 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside, hyperoside, isorhamnetin 3-O-robinobioside, and scoparone from A. capillaris using a binary gradient of acetonitrile : 0.1% trifluoroacetic acid with UV detection at 254 nm. The scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-O-robinobioside (5), and scoparone (6) contents of the herb of A. capillaris collected from fifteen district markets in Korea were 0.00~0.90 mg/g, 0.06~7.29 mg/g, 0.06~0.91 mg/g, 0.07~5.05 mg/g, 0.42~13.11 mg/g, and 1.11~29.82 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of A. capillaris.

• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.189-193
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• 2007

We investigated the changes of growth characteristics and major components according to harvesting times in Artemisia capillaris. Flower buds farmed on July 30 and flowers were all open on August 30 and seeds were mature in September, As the growth by harvesting time was the best on August 30 so fresh weight and dry weight were the highest. Especially, in this time, plants had no leaves and fresh weight was investigated as 243.7 g composed of 109.6 g capitulum and 134.1 g stem. Scoparone content, a major component, was the highest as 6.50 mg/g DW in capitulum on August 30. Also capillarisin appeared in both leaf and capitulum except stem and capitulum was shown the most capillarisin content as 1.65 mg/g DW on July 30.

• Natural Product Sciences
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• v.24 no.2
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• pp.109-114
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• 2018

Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.

• Korean Journal of Medicinal Crop Science
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• v.16 no.1
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• pp.57-61
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• 2008

This experiment was carried out to search for changes of growth and pharmacological component contents by cultivation years in Artemisia capillaris. Percentage of ovenwintering in 1-year old was high as 95.3% but that in 2-years old was very low as 64.2%. Plant height was the highest in 2-years old among the treatments. By the way, number of stems and branches were shown wide difference by cultivation years, so those were the best in 3-years old. It was thought that 3-years old plants dispersed and used their nutrients by increasing branches. Fresh weight was the highest as 200.0 g in 3-years old, whereas weight of capitulum included scoparone, effective component, was the highest as 109.6 g in 2-years old and low as 96.4 g in 3-years old. In general components, crude protein and ash were more included in 1-year old and crude lipid and fiber were more included in 2 and 3-years old. Especially, content of crude fiber in stem was the highest in 3-years old. Pharmacological components were the most existed in capitulum of 2-years old, so contents of scoparone and capillarisin were 6.5 mg/g DW, 1.65 mg/g DW, respectively.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.187.1-187.1
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• 2001

• Archives of Pharmacal Research
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• v.25 no.4
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• pp.469-474
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• 2002

The inner shell of the chestnut (Castanea crenata S. et Z., Fagaceae) has been used as an anti-wrinkle/skin firming agent in East Asia, and preliminary experiments have found that a 70% ethanol extract from this plant material can prevent cell detachment of skin fibroblasts from culture plates. In order to examine the molecular mechanisms underlying this phenomenon, its effects on the expression of adhesion molecules, such as fibronectin and vitronectin, were investigated using the mouse skin fibroblast cell line, NIH/3T3. Using fixed-cell ELISA, Western blotting and immunofluorescence cell staining, it was clearly demonstrated that the chestnut inner shell extract enhanced the expression of the cell-associated fibronectin and vitronectin. Scoparone (6,7-dimethoxycoumarin), isolated from the extract, also possessed similar properties. These findings suggest that the enhanced expression of the adhesion molecules may be one of the molecular mechanisms for how the chestnut inner shell extract preventing cell detachment and may be also responsible for its anti-wrinkle/skin firming effect.

• Korean Journal of Pharmacognosy
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• v.50 no.2
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• pp.133-140
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• 2019

In our previous study, we found uracil, adenosine, protocatechuic acid, syringin (eleutheroside B) and scoparone (6, 7-dimethoxycoumarin) in the Oplopanax elatus Nakai Stem. High-performance liquid chromatography (HPLC) -UV was used to quality and quantify the internal marker compounds in the O. elatus extract after validation of method with linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy and precision. The specificity assessment visually confirmed that the substance was detected without the introduction of other substances. The established method showed high linearity of the calibration curve and coefficient of correlation ($R^2$) of over the 0.999. HPLC was reported as five standard compounds equivalent using the following linear equation based on the calibration curve. The accuracy of measurement was 84.34 ~ 119.74% and the relative standard deviation (RSD) value was 0.28 ~ 1.60%. In addition, our established method showed high repeatability. The RSD value was 1.10 ~ 6.81%. So, we found the amount of the internal marker compounds in the O. elatus extract. These results demonstrated that can be used to quality evaluation of the O. elatus.