• 식물병연구
• /
• 제9권1호
• /
• pp.47-51
• /
• 2003

남양주, 양주, 양평에서 파드득나물 균핵병의 발생생태를 조사한 결과, 균핵병은 늦가을부터 초봄까지 주로 동계기간에 발생했으며, 지역별로는 양평에서 발생이 심했다. 포장에서 분리한 파드득나물 균핵병균은 기보고된 Sclerotinia sclerotiorum의 균학적 특성과 일치하였고, 균사생장은 5~3$0^{\circ}C$에서 이루어졌으며, 15~3$0^{\circ}C$에서 활발하게 생장하였다 균핵형성은 20~$25^{\circ}C$에서 많이 형성되었으며, 낮은 온도에서 형성된 균핵의 크기가 큰 경향이었다. 균핵생존율은 균핵크기가 작을수록(~2 mm > 3~4 mm > 5 mm~) 높았으며, 치상온도에서의 정치시간이 길수록 높게 나타났다.

• Mycobiology
• /
• 제29권3호
• /
• pp.154-159
• /
• 2001

Natural sclerotium formation in two species of Curvularia was observed. The sclerotia were spherical to elongated. The scanning electron microscopical observations of sclerotia revealed that the sclerotia were of two distinct layers of cells, outer loosely woven hyphae and inner contact layer of cells. Different lights, viz. red, blue, green, fluorescent or addition of culture filtrate of Sclerotium rolfsii in the medium did not affect sclerotium formation.

• 한국응용곤충학회지
• /
• 제15권3호
• /
• pp.153-159
• /
• 1976

Sclerotinia sclerotiorum(Lib.) de Bary의 상치, 오이 및 유채의 3균주에 대하여 PDA를 기본부지로하여 광선이 이들의 균핵형성에 미치는 영향을 연구하였다. 광원으로서는 주광색 형광등을 사용하였다. 그 결과를 요약하면 다음과 같다. 1) 계속광처리에서 광도 480Lux까지는 광도가 증가함에 따라 증가했지만 성숙균핵의 건물중은 이와는 반대로 감소하였다. 그리고 800 Lux 처리에서는 균핵시원체의 유기가 크게 억제되었고 성숙균핵은 거의 형성되지 않았다. 2) 5000Lux의 고광도라 하더라도 48시의 단시간처리는 균핵시원체수 및 성숙균핵수를 증가시켰다. 그러나 성숙균핵의 건물중은 160 Lux에서만 다소 증가하는 경향이 있었다. 3) 광 shock 즉 1분간치의 계속광 48시간까지의 160 Lux, 500Lux 처리는 모두 균핵형성수와 건물중을 증가시켰는데 균핵형성수와 건물중을 증가시켰는데 균핵형성수는 500Lux에서 균핵건물중은 160Lux에서 더 높았다.

• Mycobiology
• /
• 제33권1호
• /
• pp.1-6
• /
• 2005

Morphological characteristics of hyphal interaction between Grifola umbellata (Pers. Ex Fr.) Pilat and its companion fungus which related to sclerotia formation from hyphae were investigated by external observations, light microscopy and transmission electron microscopy (TEM). External observations showed that a dense antagonism line was formed by both G. umbellata and companion fungus after their hyphae contacted each other in dual culture. Many hyphal strands emerged on the colony of G. umbellata and differentiated to sclerotia from where hyphal strands crossed. Light microscope observations revealed the process of antagonism line formation. Mature antagonism with structural differentiation, was composed of three main layers: the rind, the rind underlayer and the hypha layer. TEM observations showed that after colonies hyphal contact, a series of reactions always occurred in both G. umbellata and companion fungus. Cells in the center of antagonism line were dead. Cells of G. umbellata adjacent to the antagonism line were usually large and hollow, with unilateral thickened wall, whereas those of companion fungus were empty, with thin or thick wall. Both hyphal interaction at the antagonism line may be one of the main reasons for sclerotia of G. umbellata differentiation from hypha.

• 한국자원식물학회지
• /
• 제11권3호
• /
• pp.296-300
• /
• 1998

When medicinal mushroom, Poria cocos, is cultured , inoculation method of spawn is cross slice inoculation of which the both sides of pine tree were peeled and spawn of P.cocos was inoculated. However, this method required lots of inoculation amount. This study was carried out to improve the culturing method of P. cocos. A good growth of P.cocos was observed in MCM(mushroom complete medium), showing proper mycelia growth and density. In inoculation amount, conventional method(cross slice inoculation) requires 20 bottles of spawn. In contrast, short log method required 8 bottles of spawn and drilling inoculation method 2~3 bottles, which could save by 60% and 85-90% respectively. In the selectrion of tree species, pine and larch had better condition for spawn culture and sclerotia formation condition.In terms of yield , pine was 33.7kg/3.3$m^2$. In the yield of pine, conventional method was 23.4kg/3.3$m^2$, drilling inoculation 29.4kg/3.3$m^2$, short log inoculation 31.7kg/3.3$m^2$, therefore drilling inoculation could increase by 25% and short log inoculation 35%, In addition, management cost was also saved.

• The Plant Pathology Journal
• /
• 제23권3호
• /
• pp.212-214
• /
• 2007

In 2005 and 2007, a basal stem rot of Disporum smilacinum caused by Sclerotium rolfsii occurred sporadically in a herb farm at Hamyang, Korea. The symptom initiated with water-soaking lesion and progressed into stem rot and wilt of a whole plant. Severely infected plants were blighted and died eventually. White mycelial mats appeared on the lesion at early stage and a number of sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1-3 mm in size and white to brown in color. The optimum temperature for the growth and sclerotia formation was 30 on PDA and the hyphal width was measured $3-8{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of symptom, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of D. smilacinum caused by S. rolfsii in Korea.

• Mycobiology
• /
• 제39권3호
• /
• pp.230-232
• /
• 2011

In this study, we characterized sporadically occurring sclerotium rot caused by Sclerotium rolfsii in Chinese chive (Allium tuberosum Roth.) in farm fields in Sacheon, Korea. The initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. Further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the scaly stem and leaves. The sclerotia were globoid, 1~3 mm, and white to brown. The optimum temperature for growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$. The diameter of the hypae ranged from 4 to 8 ${\mu}m$. Clamp connection was observed on PDA medium after 5 days of incubation. Based on the mycological characteristics, internal transcribed spacer sequence analysis, and pathogenicity test, the causal agent was identified as Sclerotium rolfsii Saccardo. This is the first report of sclerotium rot in Chinese chive caused by S. rolfsii in Korea.

• Mycobiology
• /
• 제38권2호
• /
• pp.156-158
• /
• 2010

Stem rot disease was found in garlic (Allium sativum L.) cultivated from 2008 to 2010 in the vegetable gardens of some farmers in Geumsan-myon, Jinju City, Gyeongnam province in Korea. The initial symptoms of the disease were typical water-soaked spots, which progressed to rotting, wilting, blighting, and eventually death. White mycelial mats had spread over the lesions near the soil line, and sclerotia had formed over the mycelial mats on the stem. The sclerotia were globoid in shape, 1~3 mm in size, and tan to brown in color. The optimum temperature for growth and sclerotia formation on potato dextrose agar (PDA) medium was $30^{\circ}C$. The diameter of the hyphae ranged from approximately 4 to $8\;{\mu}m$. Typical clamp connection structures were observed in the hyphae of the fungus, which was grown on PDA medium for 4 days. On the basis of the mycological characteristics and pathogenicity of the fungus on the host plants, the causal agent was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot disease in garlic caused by S. rolfsii in Korea.

• Mycobiology
• /
• 제37권2호
• /
• pp.158-159
• /
• 2009

In 2007 to 2008, a fruit rot of Melon (Cucumis melo L.) caused by Sclerotium rolfsii occurred sporadically in a farmer's vinyl house in Jinju City. The symptoms started with watersoaking lesion and progressed into the rotting of the surface of fruit. White mycelial mats appeared on the lesion at the surface of the fruit and a number of sclerotia formed on the fruit near the soil line. The sclerotia were globoid in shape, 1${\sim}$3 mm in size, and white to brown in color. The hyphal width was measured 3 to 8 ${\mn}$. The optimum temperature for mycelial growth and sclerotia formation was 30 on PDA. Typical clamp connections were observed in hyphae of grown for 4 days on PDA. On the basis of symptoms, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of the fruit rot of Melon caused by S. rolfsii in Korea.

• 한국균학회지
• /
• 제26권3호통권86호
• /
• pp.396-398
• /
• 1998

16종의 원목에 저령을 인공접종한 결과, 때죽나무의 원목에서 $3{\sim}5\;mm$ 크기의 조밀한 균핵이 다수 관찰되었으며, 왕성한 균사의 밀도를 나타내었다. 균핵의 형성은 16종의 원목 중 6종의 원목에서 관찰되었다.