• BMB Reports
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• v.32 no.4
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• pp.379-384
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• 1999

Taxol, a natural product with significant anti-tumor activity, stabilizes microtubules and arrests cells in the G2/M phase of the cell cycle. It has been reported that taxol has additional effects on the cell such as an increase in tyrosine phosphorylation of proteins and activation of mitogen-activated protein (MAP) kinase. This phosphorylated kinase translocates into the nucleus and phosphorylates its substrate c-jun, c-fos, ATF2, and ATF3. The MAP kinase family is comprised of key regulatory proteins that control the cellular response to both proliferation and stress signals. First examination was cytotoxicity and apoptosis-induced concentration with paclitaxel in HeLa cell. A half-maximal inhibition of cell proliferation ($IC_{50}$) occurred at 13 nM paclitaxel. When DNA fragmentation was analyzed by agarose gel electrophoresis, a nucleosomal ladder became evident 24 h after a taxol (50 nM) addition to the cells. In addition, an apoptotic body was detected by electron microscopy. Taxol-treated cells were arrested at the S phase at 10 nM. Treatment of 50 nM taxol activated the extracellular signal-regulated protein kinase (ERK1), and a fraction of the activated MAP kinases entered the nucleus. It was also discovered that nucleus substrates c-jun was phosphorylated and activated in the cell. The activated ERK1 could subsequently translocate into the nucleus and phosphorylate its substrate c-jun as well. This study suggests that taxol-induced apoptosis might be related with signal transduction via MAP kinases.

• Journal of Environmental Science International
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• v.29 no.12
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• pp.1249-1260
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• 2020

Non-point source pollutants have characteristics the render them difficult to manage owing to the uncertainty of flow paths. As agricultural non-point sources account for more than 57% of non-point source pollutants, the necessity for management is increasing. This study examines the possibility of utilizing land cover maps to suggest a more appropriate method of setting management priority for agricultural non-point sources in the Daecheong Lake area and draws implications by comparing the results derived using the cadastral map, as mentioned in the TMDL Basic Policy. To define the prioritized areas for management, the pollution load was calculated for each subbasin using the formula from the TMDL technical guidelines. As a result, the difference in the average pollution load between the land cover map and cadastral map ranged from 11.6% to 21% among the subbasins. In almost all subbasins, there were differences in the ranking of management priorities depending on the land information that was used. In addition, it was found that it was reasonable to use the level 3 land cover map to calculate the load generated by the land system for examining the implementation goals and methods of each data and comparing them with satellite images.

• Genomics & Informatics
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• v.1 no.2
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• pp.108-112
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• 2003

We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific $F_2$ population of 107 plants with 320 RFLP, 136 AFLP, and 46 SSR markers. The resulting linkage map consists of 15 linkage groups covering 1,720 cM with an average map distance of 3.7 cM between framework markers. Most RFLP markers ($80\%$) were pepper-derived clones and these markers were evenly distributed all over the genome. Genes for defense and biosynthesis of carotenoids and capsaicinoids were mapped on this linkage map. By using 30 primer combinations, AFLP markers were generated in the $F_2$ population. For development of SSR markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. This combined map provides a starting point for high-resolution QTL analysis, gene isolation, and molecular breeding.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.12
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• pp.1115-1121
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• 2012

This paper proposes a method to extract accurate plane of an object in unstructured environment for a humanoid robot by using a laser scanner. By panning and tilting 2D laser scanner installed on the head of a humanoid robot, 3D depth map of unstructured environment is generated. After generating the 3D depth map around a robot, the proposed plane extraction method is applied to the 3D depth map. By using the hierarchical clustering method, points on the same plane are extracted from the point cloud in the 3D depth map. After segmenting the plane from the point cloud, dimensions of the planes are calculated. The accuracy of the extracted plane is evaluated with experimental results, which show the effectiveness of the proposed method to extract planes around a humanoid robot in unstructured environment.

• Journal of Information Processing Systems
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• v.12 no.3
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• pp.538-547
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• 2016

Cloud computing is a new style of computing in which dynamically scalable and reconfigurable resources are provided as a service over the internet. The MapReduce framework is currently the most dominant programming model in cloud computing. It is necessary to protect the integrity of MapReduce data processing services. Malicious workers, who can be divided into collusive workers and non-collusive workers, try to generate bad results in order to attack the cloud computing. So, figuring out how to efficiently detect the malicious workers has been very important, as existing solutions are not effective enough in defeating malicious behavior. In this paper, we propose a security protection framework to detect the malicious workers and ensure computation integrity in the map phase of MapReduce. Our simulation results show that our proposed security protection framework can efficiently detect both collusive and non-collusive workers and guarantee high computation accuracy.

• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.45-52
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.235-237
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• 2003

GIS became increasingly important in information-oriented society as social indirect capital and many GIS data are developed. To use these data effectively standard format that enables easy to transport and store is needed. For this purpose, OGC developed GML based on XML as web standard format of geographical information. In this study, web based mapping with respect to digital geologic map and gravity anomaly map was accomplished using GML. While, styling methods were implemented in XSLT to make the visualizing suitable for the character of each layer, so it is possible to make dynamic maps in the SVG. GML-base map produced in this study can be transferred and represented without loss of the meaning and degrading on the web.

• Journal of the Semiconductor & Display Technology
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• v.21 no.3
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• pp.17-21
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• 2022

A variety of techniques have been proposed in the literature for depth improvement in depth from focus method. Unfortunately, these techniques over-smooth the depth maps over the regions of depth discontinuities. In this paper, we propose a robust technique for improving the depth map by employing a nonconvex smoothness function that preserves the depth edges. In addition, the proposed technique exploits the mutual structures between the depth map and a guidance map. This guidance map is designed by taking the mean of image intensities in the image sequence. The depth map is updated iteratively till the nonconvex objective function converges. Experiments performed on real complex image sequences revealed the effectiveness of the proposed technique.

• Journal of the Mineralogical Society of Korea
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• v.31 no.4
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• pp.257-266
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• 2018

East China Sea (ECS) is known to be supplied with large amounts of sediments form Huanghe, Changjiang and various rivers in Korea. Many studies have been conducted to identify the effects of rivers and deposition process of ECS, but no consensus has been reached. In this study, clay minerals, rare earth elements (REEs) and grain size were analyzed to study the provenance and sedimentation environment of core 99MAP-P63 in ECS. Clay mineral contents of 99MAP-P63 are abundant in order of illite, chlorite, kaolinite, and smectite. The provenance of 99MAP-P63 sediments using clay minerals is interpreted as the Changjiang regardless of depth. As a result of REEs analysis, 99MAP-P63 sediments are very similar to Chinese rivers sediments. Therefore, the provenance of 99MAP-P63 is Changjiang, and the influence of Korean river seems to be insignificant. 99MAP-P63 sediments are generally classified as sandy silt, but the top of the core is divided into sand with a sand contents of 85 %. Compared with surrounding cores, sandy silt sediments arecorresponded to the low stand stage when sea-level was low, and the sediments were thought to have been supplied directly through the paleo-Changjiang. Sandy sediments in uppermost of core are corresponded to transgressive stage. Although distance from estuary was increased due to sea-level rise, it was possible to supply coarse sediments due to high bottom stress, and the paleo-Changjiang sediments deposited in study area were re-deposited.

• Journal of Embryo Transfer
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• v.28 no.1
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• pp.57-61
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• 2013

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.