• Title/Summary/Keyword: science laboratory

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Colonization of Pathogens in Earphones and Observation of Effective Sterilization Methods and Cycles

  • Kwon, Hyeokjin;Jeong, Myeongguk;Go, Shinjee;Kim, Yeojin;Kim, Yein;Kim, Yeeun;Roh, Seungjun;Lee, Seonggwang;Choi, Go-Eun
    • Biomedical Science Letters
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    • v.28 no.3
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    • pp.186-191
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    • 2022
  • The use of earphones has recently been widely used around the world. In currently, students wear earphones a lot in a daily life. The types of earphones are open-earphones, Canalphones, and headphones. Many students don't periodically to sterilization their earphones. Therefore, it can be an incubator that can induced ear infections. The objective of this study was to detect the pathogenic bacteria from the earphones used by the students. A total of 3 type earphones swabs were collected by sterile cotton swabs. The swabs were inoculated onto BHI agar and incubated aerobically 48 hour at 37℃. 16s rRNA PCR, electrophoresis and sequencing were performed to confirm the identification of all the bacterial isolates. As a result, 24 pathogens were identified in sequencing. Three types of earphones were sterilized in three ways: ultraviolet (UV), 70% ethyl alcohol, and antibacterial wet tissue. If you use earphones for a long time without disinfecting them for a long time, it causes various diseases such as external ear infections. The findings of this study the users periodically to sterilization their respective earphones.

Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

  • Pongsomboon, Siriporn;Tang, Sureerat;Boonda, Suleeporn;Aoki, Takashi;Hirono, Ikuo;Yasuike, Motoshige;Tassanakajon, Anchalee
    • BMB Reports
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    • v.41 no.9
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    • pp.670-677
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    • 2008
  • A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly ($\leq$ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.

Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets

  • Kong, Xiangfeng;Yin, Yulong;Wu, Guoyao;Liu, Hejun;Yin, Fugui;Li, Tiejun;Huang, Ruilin;Ruan, Zheng;Xiong, Hua;Deng, Zeyuan;Xie, Mingyong;Liao, Yiping;Kim, Sungwoo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.9
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    • pp.1453-1461
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    • 2007
  • This study was conducted to test the hypothesis that dietary supplementation with an herbal extract of Acanthopanax senticosus (AS) enhances the immune response in weaned piglets. Sixty piglets weaned at 21 days of age were randomly assigned to 3 treatment groups representing the addition of 0 or 1 g/kg of the AS extract or 0.2 g/kg of colistin (an antibiotic) to maize- and soybean meal-based diets (n = 20 per group). On days 7, 14 and 28 after initiation of the addition, total and differential counts of leucocytes, proliferating activity of peripheral lymphocytes, serum levels of immunoglobulins (Ig) and cytokines and the spleen index were determined. The AS extract decreased (p<0.05) the number of neutrophils on days 7 and 28 in comparison with the control group and reduced (p<0.05) serum interleukin-$1{\beta}$ level on day 28 compared with the other 2 groups. Dietary supplementation with the AS extract increased (p<0.05) the lymphocyte/leukocyte ratio on day 28 compared with the control group and increased the proliferating activity of lymphocytes on days 14 and 28 compared with the other 2 groups. The AS extract increased (p<0.05) the serum content of IgG on day 7 and of IgG and IgM on day 28 compared with the other 2 groups, as well as increasing the serum content of tumor necrosis factor on day 7 and spleen index on days 7 and 28 compared with the control group. Collectively, these findings suggest that the AS extract as a dietary additive enhances the cellular and humoral immune responses of weaned piglets by modulating the production of immunocytes, cytokines and antibodies.

A Survey of Science Teachers, Perception and Attitudes on Disposal of School Laboratory Waste Water and Materials (환경 오염 및 과학 실험실 폐수와 폐기물 처리에 대한 과학 교사들의 인식과 태도 조사)

  • Kim, Ik-Hee;Park, Kuk-Tae;Jeong, Jin-Su
    • Hwankyungkyoyuk
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    • v.20 no.4
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    • pp.65-83
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    • 2007
  • The purpose of this study was to survey science teachers, perception and attitudes on disposal of school laboratory waste water and materials. To fulfill this aim, a questionnaire was administered to 244 secondary school science teachers. The result of this study revealed that there was no large disparity in the degree of understanding of laboratory waste disposal and the laboratory waste water treatment, and in secondary science teachers' perception and practicing attitude on the environment. While science teachers had deep understanding of laboratory waste disposal and its influence on environmental pollution, they expressed less positive attitude toward the actual disposal of waste materials and waste water, and participation in teacher training. Therefore, it is necessary to provide standards of systematized disposal methods and proper disposal facilities in middle and high school laboratories, as well as to investigate teachers' needs before providing teacher training program.

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