• 제목/요약/키워드: science laboratory

검색결과 16,196건 처리시간 0.042초

In Vitro Evaluation of Swine-Derived Lactobacillus reuteri: Probiotic Properties and Effects on Intestinal Porcine Epithelial Cells Challenged with Enterotoxigenic Escherichia coli K88

  • Wan, Zhilin;Wang, Li;Chen, Zhuang;Ma, Xianyong;Yang, Xuefen;Zhang, Jian;Jiang, Zongyong
    • Journal of Microbiology and Biotechnology
    • /
    • 제26권6호
    • /
    • pp.1018-1025
    • /
    • 2016

  • Probiotics are considered as the best effective alternatives to antibiotics. The aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. A total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. Sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adherence to intestinal porcine epithelial cells (IPEC-1). One isolate, LR1, identified as Lactobacillus reuteri, was selected for extensive study of its probiotic and functional properties in IPEC-1 cell models. L. reuteri LR1 exhibited good adhesion to IPEC-1 cells and could inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells. L. reuteri LR1 could also modulate transcript and protein expression of cytokines involved in inflammation in IPEC-1 cells; the Lactobacillus strain inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-α) and protein (IL-6), and increased the level of anti-inflammatory cytokine (IL-10). Measurement of the permeation of FD-4 showed that L. reuteri LR1 could maintain barrier integrity in monolayer IPEC-1 cells exposed to ETEC. Immunolocalization experiments showed L. reuteri LR1 could also prevent ETEC-induced tight junction ZO-1 disruption. Together, these results indicate that L. reuteri LR1 exhibits desirable probiotic properties and could be a potential probiotic for use in swine production.

  • https://doi.org/10.4014/jmb.1510.10089 인용 PDF KSCI KPUBS HTML

150MW 펄스 MODULATOR의 설계 및 동작특성 (Design and Operational Charcteristics of 150MW Pulse Modulator)

  • 박성수;오종석;이경태;김상희;손윤규;최관;장성덕;박상욱;남상훈;조무현;남궁원
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 1992년도 하계학술대회 논문집 B
    • /
    • pp.928-930
    • /
    • 1992

  • The design beam energy of PLS(Pohang Accelerator Laboratory) Linac is 2Gev. The linac employs total 11 units of modulators and klystrons. The maximum peak output powers of the modulators are 200MW (400kV, 500A, 4.4$\mu$S flat-top, 800$\Omega$ load) to drive the klystrons which have the peak microwave power of 80MW. Prior to the development of the 200MW modulators, a prototype 150MW modulator has been constructed and tested. We have achieved output pulses of 350kV, 420A and 3.5$\mu$S flat-top with 840$\Omega$ water load. In this article, the test results and computer simulations of charging, De-Q'ing, and discharging are presented.

  • PDF

Microbiology of a Mediator-less Microbial Fuel Cell

  • Kim, Byung-Hong;Chang, In-Seop;Park, Hyung-Soo;Lee, Ji-Young;Thu Nguyet Phung
    • 한국미생물생명공학회:학술대회논문집
    • /
    • 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
    • /
    • pp.75-79
    • /
    • 2004

  • PDF