• Title/Summary/Keyword: science experiment

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Comparison of Anesthetic Effects Induced by Tiletamine-Zolazepam and Azaperone Plus Tiletamine-Zolazepam in Growing Pigs (돼지에서 Tiletamine-Zolazepam 단독과 Azaperone, Tiletamine-Zolazepam 합제의 마취 효과에 대한 비교)

  • Kim, Young-Suk;Kim, Myung-Jin;Lee, Soo-Jin;Lee, Jae-Il;Jun, Moo-Hyung;Park, Chang-Sik;Kim, Myung-Cheol
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.300-304
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    • 2007
  • The purpose of this study was to determine the anesthetic effects of tiletamine-zolazepam (TZ) alone and azaperone plus tiletamine-zolazepam in growing pigs, and to compare the various physiological parameters in both treatments. Cross experiment was accomplished at 2-week interval. Group 1 (TZ group): six pigs ($31.4{\pm}4.83$ kg) received 4.4 mg/kg of TZ alone. Group 2 (ATZ group); the same six pigs ($43.6{\pm}4.31$ kg) received 4.4 mg/kg of TZ twenty minutes after receiving 2 mg/kg of azaperone. All of the anesthetic drugs were injected into the trapezius muscles. The pigs were fasted for 24 hours before the experiments. Induction and recovery values were determined. Heart rate, respiratory rate, rectal temperature, $pO_2,\;pCO_2$ and pH were determined before administration and 5, 25, 45, 65 and 85 minutes after administration. Induction time of ATZ group was more rapid than that of TZ group (p<0.01). During recovery, sternal recumbency time, standing time and walking time of ATZ group were longer than those of TZ group (p<0.01). Heart rate, respiratory rate, $pO_2,\;pCO_2$, and pH did not show especial differences between the two groups. However, rectal temperature was significantly different between the TZ and ATZ group (p<0.05). As a result, ATZ group had a faster induction and a longer duration of anesthesia than TZ group did. Thus, it was concluded that ATZ combination could be usefully used for chemical restraint in pigs.

The Effect of the Optical Points Difference between Finished-Reading Glasses and Dispensing Reading Glasses (완성품 돋보기와 조제가공된 돋보기가 광학적 요소에 미치는 영향)

  • Shim, Young-Cheol;Yoo, Gun-Chang;Kim, In-Suk
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.3
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    • pp.65-71
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    • 2008
  • Purpose: This paper studied the effect of eyes on the comparison between the distance optical centers problem of dispensing reading glasses made by optician and finished reading glasses in the current market. Methods: The method of this study has been measured by eleven different categories from +1.00D to +4.00D. This study also separated into three groups by their optical frame size and measured optical center point (O.C) and optical center height (O.H) with 200 peoples of man and females over 40 years old without ocular disease living in Gwang-san gu, Gwang-Ju city. Results: As a result, optical center point ranged from 57 mm to 80 mm and it turned to be most common range is from 61 mm to 65 mm (64.6%). Moreover, the optical center height ranged from 1 mm to 8mm and most common ranged (23%) were 4 mm. In other words, finished reading glasses have irregular optical ranges. After observing 200 people who are over 40 years old men and women, result shows that more than 75.5% (151 people) currently use finished reading glasses. Survey of 151 people, most common error between the finished reading glasses's O.C and the wearers P.D were 4 mm (45%). Furthermore, the most common error between the finished reading glassses's O.H and the wearers O.H ranged from 3 mm to 4 mm. Astonishingly, the entire 151 people who wear finished reading glasses appeal that they feel tiredness on their eyes when they wear finished reading glasses. 53 people (35%) claimed that they feel tiredness on their eyes after 10 to 20 minutes wearing finished reading glasses. Base on the research, We conducted more experiment to find the value of prism of optical centers err because it will tell us whether the finished reading glasses are good enough to wear or not. We multiplied diopter by the difference between finished reading glasses's O.C. and wearer's P.D. Consequently, We found out that the finished reading glasses counter to the German RAL-RG 915 policy. And We also found that it is relative to the diopter of lenses. In conclusion, based on the researched that wearing finished reading glasses have a dangerous factor for our vision. Therefore optician must need to recommend correctly made dispensing reading glasses based on the optical center point.

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Analysis of Chinese Consumer Preference of Country of Origin for Apples based on National Organic Certification (사과의 국가별 유기인증 결합에 대한 중국 소비자 선호분석)

  • Kwon, Jae-Hyun;Kim, Jeong-Nyeon;Hong, Na-Kyoung;Kim, Tae-Kyun
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.4
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    • pp.225-230
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    • 2014
  • This study investigates the effect of organic certification of apples on consumer preference in China as a way to support the expanded export of Korean apples to China. A choice experiment was designed to analyze the apple consumption in China. A total of 298 Chinese consumers answered the survey, and multinomial logit models were used to analyze the results. Organic certification was identified as an important determinant of consumer preference for apples in China, affecting both the evaluation and choice of country of origin. The results also indicated that Korean organic certification significantly increased the probability of Chinese consumers choosing Korean apples. Thus, organic certification by the Korean government should be strengthened to promote apple exports to China, plus the results of this study may provide useful information to promote agricultural product exports and improve the organic certification system.

Photomixotrophic Growth of Solanum tuberosum L. in vitro with Addition and Omission of Organic Materials at Thee Initial Sucrose Levels in the Medium (세 수준의 자당이 첨가된 배지에서 유기물의 첨가 유무에 따른 Solanum tuberosum L.의 기내 광혼합영양생장)

  • Jeong, Byoung-Ryong;Yang, Chan-Suk;Kim, Gyeong-Hee;Park, Young-Hoon;Kozai, Toyoki
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.51-55
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    • 2004
  • The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$ $m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$ $m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$ $m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$ $m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$ $d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.

The Effects of Aroma Inhalation Method with Roll-on in Occupation Stress, Depression and Sleep in Female Manufacture Shift Workers (아로마 롤온흡입법이 생산직 여성 교대근로자의 직무스트레스, 우울 및 수면에 미치는 효과)

  • Oh, Hyun-Mi;Jung, Geum-Sook;Kim, Ja Ok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.5
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    • pp.2903-2913
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    • 2014
  • The purpose of this study was to examine the effects of aroma inhalation method with roll-on in occupation stress, depression and sleep in female manufacture shift workers. The study was a nonequivalent control group pre-post test design. The subjects in this study were female manufacture shift workers who were from K-do area; 26 in the experiment group and 26 in the control group. Data was collected from April 1 to May 25. The questionnaires about pre and post occupation stress, depression and sleep were used to identify the effect of aroma inhalation method with roll-on. The data was analyzed using SPSS Win 18.0 version program. The homogeneity between two groups of general characteristics was analyzed by Chi-square test and independent t-test. The homogeneity of occupation stress, depression and sleep were analyzed by independent t-test. The difference of pre-post occupation stress, depression and sleep were analyzed by independent t-test. The finding of this study were as follows: The first research hypothesis that the occupation stress of the experimental group might get less than the control group was rejected(t=-0.37, p=.717). The second research hypothesis that depression of the experimental group might get less than the control group was accepted(t=-6.11, p<.001). The third research hypothesis that sleep of the experimental group might get less than the control group was accepted(t=3.15, p=.003). Based on the above-mentioned findings, Aroma inhalation method with roll-on can be utilized as an effective intervention for depression and sleep in female shift workers in the manufacturing industry. and The multiple nursing intervention should be needed to decrease on occupation stress in female manufacture shift workers.

Detection of Auxotrophic Mutants form Valsa ceratosperma, the Causal Fungus of Apple Canker (사과나무 부란병균(腐爛病菌) Valsa ceratosperma에서의 Auxotrophic Mutants의 검출(檢出))

  • Hong, Yeon Gyu;Uhm, Jae Youl
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.119-126
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    • 1987
  • This study was conducted to elucidate the most appropriate method to obtain auxotrophic mutants from Valsa ceratosperma, the causal fungus of apple canker, which may be used as a gene marker in detecting the transfer of the factors of avirulent strains to virulent strains. Among the 3 kinds of synthetic media tested, each have two formula for minimal and complete, the medium which has been used in study of Endothia parasitica (E. P medium) was turned out to be most appropriate for the growth of V. ceratosperma. A medium for single colony formation from pycnidiospore of this fungus was developed by adding 0.5% L - sorbose to the E. P minimal medium. The period of incubation in dark for preventing the photoreactivation after U. V irradiation was estimated as about 60hrs at which most of the spores become binucleate. Largest number of putative auxotrophs were obtained at about 50second of irradiation to the spores smeared on the medium for single colony formation, at which the survival rate of spores was 5 to 6 percent. With these method developed in this experiment, 161 isolates of putative auxotrophs were detected among which the nutrient requirement for 10 isolates were determined. Five out of 10 mutants were still virulent to apple tree and all but one could not sporulate.

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The Establishment of Nitrogen Fixation by Cultured Cell-Rhizobium Association Through Tissue Culture Technique in Soybean (대두조직배양세포(大豆組織培養細胞) - Rhizobium에 의(依)한 질소고정력(窒素固定力))

  • Kang, Sang Jae;Park, Woo Churl
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.27-35
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    • 1986
  • This experiment was carried out to elucidate the factor of nitrogenase formation and to establish the nitrogen fixation system in mixed culture of cultured cells and rhizobia through tissue culture technique using three soybean varieties, Hwangkeum, Namcheon and D 68-0099 as host plants. The results obtained were as follows; The callus was induced in embryo and radicle, but not in hypocotyl. The most favorable callus induction was caused by the individual application of 2,4-D and NAA at the concentration of 2mg/1 and 4mg/1, respectively, but in case of treating both 2,4-D and kinetin, that was done at the concentration of 0.2mg(2,4-D)/0.05mg(kinetin)per liter. The growth of cultured cell was good at the concentration of 2.0mg(2,4-D)/1 and 0.2mg(2,4-D)/0.05mg(kinetin)per liter. When cultured cells were inoculated with R. japonicum 019 and 011, their growthes were considerably inhibited. The addition of single amino acid inhibited the growth of cultured cells. Hwangkeum was inhibited considerably by methionine and leucine. The inhibition of growth by single amino acid can be abolished by the addition of certain amino acids. The differentiation of adventitious root was good at the concentration of 2.0mg 2,4-D and 0.2mg 2,4-D/0.05mg kinetin per liter. Of three host plants tested with 25 R. japonicum strains, Hwangkeum had affinity for 10 strains, Namcheon for 7 strains and D68-0099 for none. The nitrogen fixing abilities of Hwangkeum and Namcheon caused by cultured cell-Rhizobium association were high in strain 019, 007, and in 007 mixed with 119, respectively.

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Comparison of Growth Characteristics of Creeping Bentgrass(Agrostis palustris Huds.) Cultivars in Summer (하절기 크리핑 벤트그래스의 품종별 특성비교)

  • Tae, Hyun-Sook;Lee, Hyung-Seok;An, Kil-Man;Kim, Jong-Bo
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.147-156
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    • 2006
  • This study was initiated to evaluate the growth characteristics of seven creeping bentgrass cultivars in summer, 'Penncross' showed the worst visual quality, whereas 'Penn A-4' and 'Crenshaw' the best quality. 'Putter', which was maintained a fair quality during the test period, was regarded as a good cultivar because of no significant variation in summer as compared to the other caltivars. 'Crenshaw',' L-93' and 'Penn A-4' were greater in chlorophyll content and 'Penncross' lowest during the summer. Also, 'SR1020' had a low content of chlorophyll. 'Putter' greatly increased in chlorophyll content after fertilization. The highest shoot density($19.3/cm^2$) was found with 'L-93' in early August, followed by 'Crenshaw', 'Penn A-4', 'Putter', 'Dominant', and 'SR1020' in that order. However, 'Penncross' was lowest($15.7/cm^2$). As for a root length, 'L-93' was longest, being over an average 5.5cm. 'Penn A-4' and 'Putter' also showed good result in root growth. However, the root length considerably decreased with 'SR1020', 'Penncross' and 'Dominant' in summer. Brown patch was a serious disease for the most cultivars, except 'Penncross'. 'Dominant' had the most serious damage. 'Putter', 'L-93', 'Crenshaw', 'SR1020', and 'Penn A-4' were also greater in damage over the others. In regards of algae occurrence in summer, 'Penn A-4' had the least damage, while 'Dominant' the greatest. In conclusion, 'Crenshaw', 'Penn A-4' and 'L-93' were the best cultivars in terms of summer growth. Conversely, 'Penncross' was the poorest one. However, this study was conducted under the conditions of one-year old green. Accordingly, in-depth experiment should be done over several years to elucidate the characteristics of growth for the wide range of creeping bentgrass cultivars during the summer.

Effect of Packaging Systems with High CO2 Treatment on the Quality Changes of Fig (Ficus carica L) during Storage (저장 중 무화과(Ficus carica L) 선도유지를 위한 고농도 이산화탄소 처리된 포장 시스템 적용 연구)

  • Kim, Jung-Soo;Chung, Dae-Sung;Lee, Youn Suk
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.799-806
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    • 2012
  • This experiment was conducted to establish the optimum conditions for high $CO_2$ gas treatment in combination with a proper gas-permeable packaging film to maintain the quality of fig fruit (Ficus carica L). Among the fig fruits with different high $CO_2$ treatments, the quality change was most effectively controlled during storage in the 70%-$CO_2$-treated fig fruit. Harvested fig fruit was packaged using microperforated oriented polypropylene (MP) film to maintain the optimum gas concentrations in the headspace of packaging for the modified-atmosphere system. MP film had an oxygen transmission rate of about $10,295cm^3/m^2$/day/atm at $25^{\circ}C$. The weight loss, firmness, soluble-solid content (SSC), acidity (pH), skin color (Hunter L, a, b), and decay ratio of the fig fruits were monitored during storage at 5 and $25^{\circ}C$. The results of this study showed that the OPP film, OPP film + 70% $CO_2$, and MP film+70% $CO_2$ were highly effective in reducing the loss rate, firmness and decay occurrence rate of fig fruits that were packaged with them during storage. In the case of using treatments with packages of OPP film and OPP film+70% $CO_2$, however, adverse effects like package bursting or physiological injury of the fig may occur due to the gas pressure or long exposure to $CO_2$. Therefore, the results indicated that MP film containing 70% $CO_2$ can be used as an effective treatment to extend the freshness of fig fruits for storage at a proper low temperature.

A Study on the Optimal Angle as Modified Tangential Projection of Knee Bones (무릎뼈의 변형된 접선방향 검사 시 최적의 입사각에 관한 연구)

  • Oh, Wang-Kyun;Kim, Sang-Hyun
    • Journal of the Korean Society of Radiology
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    • v.15 no.6
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    • pp.919-926
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    • 2021
  • In this study, we wanted to find out the optimal angle as a modified tangential projection of the patella. In the experiment, we used Kyoto Kagaku's PBU-50 phantom. In the supine position, the F-T angle was set to 95°, 105°, 115°, 125°, 135°, 145°, and Patella tangential projection images were obtained by varying the X-ray tube angle by 5° so that the angle between the X-ray centerline and tibia at each angle was 5~20°. Image J was used for image analysis and the congruence angle, lateral patellofemoral angle, patellofemoral index and contrast to noise ratio(CNR) were also measured. SPSS 22 was used for statistical analysis, and the mean values of congruence angle, patellofemoral angle, patellofemoral index, and CNR were compared with Merchant method through one-way batch analysis and corresponding sample t-test. As a result of the study, in the case of congruence angle, the angle of incidence of the knee-angle X-ray centerline was 105°-72.5° (20° tangential irradiation), 115°-72.5°, 77.5° (15, 20° tangential irradiation), 125°-82.5° (20° tangential irradiation), lateral patellofemoral angle is 115°-72.5°, 77.5° (15, 20° tangential irradiation), 125°-72.5° (10° tangential irradiation), patellofemoral index is 115°-72.5° (15° tangential irradiation) and 125°-72.5° (10° tangential irradiation) were not significantly different from Merchant method (p> .05). In case of CNR, it is not different from Merchant method at 105°-67.5°, 72.5° (15, 20° tangential irradiation), 115°-67.5°, 72.5°, 77.5° (10, 15, 20° tangential irradiation). (P> .05). Based on the results of this study, high diagnostic value images can be obtained by setting the knee angle and the angle of incidence of the X-ray tube to 115°-72.5° (15° tangential irradiation) during the modified tangential examination of the knee bone. It was confirmed.