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Energy Status of Neurospora crassa Mutant nap in Relation to Accumulation of Carotenoids

  • Belozersk, Tatyana A.;Potapova, Tatyana V.;Isakova, Elena P.;Shurubor, Eugene I.;Savel'eva, Ludmila V.;Zvyagilskaya, Renata A.
    • Journal of Microbiology
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    • v.41 no.1
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    • pp.41-45
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    • 2003
  • N crassa mutant strain nap showed reduced growth rate, decreased electric membrane potential, and elevated intracellular ATP content in comparison to the wild type. Blue light induced a hyperpolarization of the membrane potential in both strains. The analysis of oxidative and phosphorylation activities of mitochondria isolated from the two strains has revealed that nap utilized more efficient oxidative pathways. The higher intracellular ATP content in the nap was presumably due to impaired transport systems of the plasma membrane, and to a lesser extent to the functioning of the fully competent respiratory chain. The excess ATP possibly accounts for carotenoid accumulation in the mutant.

Pesticidal Activity of Environment friendly agricultural materials (EFAMs)

  • Park, J.H.;Han, E.J.;Hong, S.J.;Ahn, N.H.;Kim, Y.K.;Jee, H.J.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.287-290
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    • 2011
  • Environment friendly agricultural material products (EFAMs) in current market were evaluated for their insecticidal or acaricidal activities. EFAMs were evaluated against Tetranychus urticae, Plutella xylostella, Myzus persicae and Nilaparvata lugens in spray method. Several EFAMs indicated high control value in spray method. Hatchability of T. urticae was relatively high after EFAM treatment. some materials show high insecticidal activities against P. xylostella larvae but not against eggs. Few mete rials showed more than 80% insecticidal activities against M. persicae and N. lugen. The control value of more than half the materials for pest control was lower than 60%. EFAMs containing S. flavescens, neem oil and Rape showed higher control value.

Determination of the effective spray- interval of Bacillus thuringiensis against diamond-back moth (Plutella xylostella) on chinese cabbage

  • Han, E.J.;Park, J.H.;Hong, S.J.;Ahn, N.H.;Jee, H.J.;Kim, Y.K.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.259-262
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    • 2011
  • In organic Chinese cabbage fields, Commercial Bacillus thuringiensis products are used widely against diamond back moth, Plutella xylostella. We conducted the study to determine the effective spray-interval of commercialized B. thuringiensis against diamond back moth on Chinese cabbages. Chinese cabbage leaves were collected 0, 1, 2, 3, 6, 10days after treatment in first trial and 0, 2, 4, 7, 9, 11days after treatment. We compared the insecticidal property of sprayed B. thuringiensis and the density of it on surface of Chinese cabbages using collected leaves. The insecticidal property maintained high until nine days after commercial B. thuringiensis products sprayed.

ON SOME MODULAR EQUATIONS AND THEIR APPLICATIONS I

  • Yi, Jinhee;Cho, Man Gi;Kim, Jeong Hwan;Lee, Seong Hoi;Yu, Jae Myung;Paek, Dae Hyun
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.3
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    • pp.761-766
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    • 2013
  • We derive several modular equations and present their proofs based on concise algebraic computations. In addition, we establish explicit relations and formulas for some parameterizations for the theta functions ${\varphi}$ and ${\psi}$ and show some applications of the modular equations to evaluations of the cubic continued fraction and the theta function ${\psi}$.

A survey of the genetic components introduced into approved GM crops (국내외 상업화 GM 작물의 유전요소 분석)

  • Woo, Hee-Jong;Chung, Chan-Mi;Shin, Kong-Sik;Ji, Hyeon-So;Lee, Ki-Jong;Suh, Seok-Chul;Kweon, Soon-Jong;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.106-114
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    • 2009
  • Genetic components introduced into approved GM crops are a key subject for safety assessment and provide a basis for the development of detection methods for GM crops. In order to understand the genetic components in approved GM crops comprehensively, we screened the genetic vector maps of GM crops that had been approved for commercialization around the world. A total of 64 varieties from 5 major GM crop species (maize, canola, cotton, soybean, and tomato) were subjected to analysis. The genetic components included genes, promoters, terminators, and selection marker. This survey may be useful for researchers who develop GM crops and methods for detecting GM crops.

Production of Colored Cocoons by Feeding Dye-Added Artificial Diet

  • Kang, Pil-Don;Kim, Mi-Ja;Jung, I-Yeon;Kim, Kee-Young;Kim, Young-Soon;Sung, Gyoo-Byung;Sohn, Bong-Hee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.22 no.1
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    • pp.21-23
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    • 2011
  • In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.

Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)

  • Kim, Sung-Wan;Yun, Eun-Young;Kim, Seong-Ryul;Park, Seung-Won;Kang, Seok-Woo;Lee, Kwang-Gill;Kwon, O-Yu;Goo, Tae-Won
    • International Journal of Industrial Entomology and Biomaterials
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    • v.21 no.2
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    • pp.151-155
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    • 2010
  • Protein disulfide isomerase (PDI) catalyzes the oxidation of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.

An artificial diet for the swallowtail butterfly, Papilio xuthus

  • Kim, Seonghyun;Hong, Seongjin;Park, Haechul;Lee, Youngbo;Park, Kwanho;Choi, Wonho;Kim, Namjung
    • International Journal of Industrial Entomology and Biomaterials
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    • v.28 no.1
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    • pp.1-4
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    • 2014
  • The effect of an artificial diet on developmental rate, a life history parameter, was examined for the swallowtail butterfly Papilio xuthus. Artificial insect diets are an essential component of many insect rearing systems that produce insects for research purposes. Complex agar-gelled diets are generally prepared in large batches and used shortly after preparation because the degradation of perishable diet ingredients, such as vitamins and fatty acids, can adversely affect insect quality (Brewer 1984). However, the timing of diet preparation may be inconvenient, and large batches wasteful, if the unused excess is discarded. The percentage of pupation varied considerably, with no significant differences among diets, on which a maximum pupation percentage of 83% was observed. Pellet-type diets were investigated with the aim of developing a more easily prepared diet. The extrusion of the artificial diet under high temperature and pressure may induce desirable chemical and physical changes in the extruded product. The purpose of the present study was to develop an artificial diet for rearing P. xuthus.

Clerodendrum ervatamioides (Lamiaceae): A new record to the flora of Vietnam

  • Heyjoong KIM;Do Van HAI;Tran Duc THIEN;Tran The BACH;Bui Hong QUANG;Duong Thi HOAN;Le Ngoc HAN;Tran Duc BINH;Ritesh Kumar CHOUDHARY;Joongku LEE
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.255-261
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    • 2022
  • Clerodendrum ervatamioides C. Y. Wu was described in 1977 with its type locality in Yunnan, China. During our floristic investigation in the Muong Nhe Nature Reserve of Vietnam of Dien Bien province and in the Muong Te district of the Lai Chau province, we collected several specimens of Clerodendrum ervatamioides, marking the first record of its occurrence in Vietnam. In this paper, details of the morphological features, distribution, ecology, photo plates, and a table comparing the morphological features with its ally Clerodendrum garrettianum Craib are provided.