• 제목/요약/키워드: school of medicine

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서울시 간호사회 가정간호시범사업 서비스 내용 및 만족도 분석 (The Contents and Satisfation of Home Care Progral Delivered by Seoul Nurses Association)

  • 임난영;김금순;김영임;김귀분;김시현;박호란
    • 대한간호
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    • 제36권1호
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    • pp.59-76
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    • 1997
  • The purposes of this study were to identify the contents and satisfaction level of the patients received home care service, and to compare the differences of the contents by the characteristics of the patients. Seventy eight patients received home care service from 1st Jan. to 30th Sept., 1996 were data-collected to analyze the contents and outcomes of home care service. Sixty-nine patients currently receiving home care service were participated to evaluate the satisfaction level of home care service. The data were analyzed using mean, standard deviation, $x^2$ test, and ANOVA by SPSS $PC^+$ program. The findings of this study were as follow : 1. The contents & outcomes of home care service 1) The mean age of the subjects was 64.4 years: 58% of them were female. Those who living in Seoul were 83% and the rest of the subjects was living in Kyung-Gi. 2) The subjects who had one diagnosis were 41%. Over 60% of them had the disease of neurologic & sensory system. 3) The mean number of visit was 6. Only one visit was 22%. The mean time of care was 79 minutes. Duration of visit from 31 minutes to 60 minutes were 47 %. The subjects who terminated the visit because of death were 67.3%. 62% of the persons who referred them to the home care service were nurses. 4) The pain after the service was more relieved than before. The amounts of intake, the degree of bed sore, edema & fracture after the service were more improved than before. Health status after the service was improved in general. 5) There were significant differences between initial and last conscious level in tracheostomy care & oxygen inhalation care. There was significant difference between initial and last degree of activity in blood sugar check. 6) There were significant differences on the number of visit in assessment of the status, evaluation & observation, vital sign check, skin care, injection, medication, bed sore care, colostomy care, relaxation therapy for pain relief, patient education, family care, exercise therapy, position change, supply of disinfected equipments and infection control. There were significant differences on visiting time in nasogastric tube care, drainage tube care and oxygen inhalation care. 2. The satisfaction level of home care service 1) 50% were male. Over 60 years of the subjects was 61 %. Those who living in Seoul were 82%. 2) The subjects who had one or two diagnosis were 32% respectively. 55% of the persons who referred them to the home care service were nurses. 3) Total level of satisfaction of home care service was very high. 4) The older the age, the higher the satisfaction level. The larger the number of visit, the higher the satisfaction level. 5) The subjects who were in cloudy state were higher level of satisfaction than in alert or coma state. The subjects whose activity were normal or who needed assistance were higher level of satisfaction than bedridden or immobilized subjects. These findings suggested that the patients had substantial need for posthospital care. They tended to be elderly and to have experienced the wide range of health problems associated with aging, chronicity, including limitations in activities, and other serious health problems. So, the nationwide home care systems beyond the limit of demonstration program by local association and the development of the effective financial system of home based health care are necessary for the clients who are in need of home care.

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도시지역 노인들의 도구적 일상생활 수행능력(IADL)에 영향을 미치는 요인 (Factors Affecting to the Instrumental Ability of Daily Living(IADL) in the Urban Elderly)

  • 이인학;문성기;김근조;박재영
    • The Journal of Korean Physical Therapy
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    • 제14권3호
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    • pp.238-272
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    • 2002
  • This study was intended to find out the factors affecting to the IADL, allowing objective assessment of physical function status of increasing elderly populations. The subjects of 635 elderly persons aged over 65 years old who live in Taejon metropolitan city were interviewed during the two-month period from June to July of 2000. The IADL of Older American Resources and Services(OARS), developed in Duke medical college of USA, was interpreted for use. The study results were statistically processed using SPSSWIN(ver 10.0) and conferred the following results: 1. Among the seven items of IADL, the women showed higher rates of 'yes' in items about using the telephone, getting to the places out of walking distance, going shopping for groceries, taking their own medicine while outnumbered by the men only in the item about managing their own money, without significant differences between them in items about preparing their own meals and doing their own housework. 2. In terms of IADL scores, $82.0\%$ of subjects showed the normal range of scores and $18.0\%$ were under the normal range, meaning dysfunction IADL. Specifications of general characteristics revealed that more dysfunctional were the male subjects, the higher age groups who are more than 75 years old, the higher educated groups, the groups who live in nursing homes, the groups without a spouse. 3. Based on daily activities, lower scores of IADL were found in the subjects who don't go out, who don't have recreational activities, who don't attend elderly gatherings in their neighborhood, who don't hold social meetings. Specifications of psychological traits revealed that more dysfunctional were IADL in the subjects who don't feel satisfied with their lives of the past or the present, who have a deep feeling of isolation, and who don't have the will to live. 4. Among 7 items of health habits, only the subjects who don't regular exercise had lower scores of IADL than those who do. According to HPI, the lower HPI, the more dysfunctional. 5. Based on the factors associated with IADL, the odds ratio of the subjects who don't live together with their families were 1.53 times that of the ones who do, who are educated 3.22 times that of the ones who are not, who don't have spouses 2.09 times that of the ones who do, who don't go out 4.35 times that of the ones who frequently go out, who don't recreational activities for an interest 2.64 times that of the ones who do, who don't attend elderly gatherings in their neighborhood 1.47 times that of the ones who do, who don't hold social meetings 2.23 times that of the ones who do, who don't feel satisfied with their present living 1.43 times that of the ones who do, who have a feeling of isolation 1.53times that of the ones who don'1, who have the weak will to live 3.21 times that of the ones who have the strong one, and who don't regular exercise 2.45 times that of the ones who do. 6. Logistic regression analysis of the study results found that such factors are significantly related as the degree of education, with/without spouse, social meetings, the will to live, and regular exercise, and that higher rates of dysfunctional subjects were in the more educated group, in the group without spouse, in the group who don't frequently go out, who don't have social meetings, who have the weak will to live, and who don't exercise.

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고려.조선시대의 수학과 사회 (Mathematics and Society in Koryo and Chosun)

  • 정지호
    • 한국수학교육학회지시리즈A:수학교육
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    • 제24권2호
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    • pp.48-73
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    • 1986
  • Though the tradition of Korean mathematics since the ancient time up to the 'Enlightenment Period' in the late 19th century had been under the influence of the Chinese mathematics, it strove to develop its own independent of Chinese. However, the fact that it couldn't succeed to form the independent Korean mathematics in spite of many chances under the reign of Kings Sejong, Youngjo, and Joungjo was mainly due to the use of Chinese characters by Koreans. Han-gul (Korean characters) invented by King Sejong had not been used widely as it was called and despised Un-mun and Koreans still used Chinese characters as the only 'true letters' (Jin-suh). The correlation between characters and culture was such that, if Koreans used Han-gul as their official letters, we may have different picture of Korean mathematics. It is quite interesting to note that the mathematics in the 'Enlightenment Period' changed rather smoothly into the Western mathematics at the time when Han-gul was used officially with Chinese characters. In Koryo, the mathematics existed only as a part of the Confucian refinement, not as the object of sincere study. The mathematics in Koryo inherited that of the Unified Shilla without any remarkable development of its own, and the mathematicians were the Inner Officials isolated from the outside world who maintained their positions as specialists amid the turbulence of political changes. They formed a kind of Guild, their posts becoming patrimony. The mathematics in Koryo significant in that they paved the way for that of Chosun through a few books of mathematics such as 'Sanhak-Kyemong', 'Yanghwi-Sanpup' and 'Sangmyung-Sanpup'. King Sejong was quite phenomenal in his policy of promotion of mathematics. King himself was deeply interested in the study, createing an atmosphere in which all the high ranking officials and scholars highly valued mathematics. The sudden development of mathematic culture was mainly due to the personality and capacity of king who took anyone with the mathematic talent into government service regardless of his birth and against the strong opposition of the conservative officials. However, King's view of mathematics never resulted in the true development of mathematics perse and he used it only as an official technique in the tradition way. Korean mathematics in King Sejong's reign was based upon both the natural philosophy in China and the unique geo-political reality of Korean peninsula. The reason why the mathematic culture failed to develop continually against those social background was that the mathematicians were not allowed to play the vital role in that culture, they being only the instrument for the personality or politics of the king. While the learned scholar class sometimes played the important role for the development of the mathematic culture, they often as not became an adamant barrier to it. As the society in Chosun needed the function of mathematics acutely, the mathematicians formed the settled class called Jung-in (Middle-Man). Jung-in was a unique class in Chosun and we can't find its equivalent in China or Japan. These Jung-in mathematician officials lacked tendency to publish their study, since their society was strictly exclusive and their knowledge was very limited. Though they were relatively low class, these mathematicians played very important role in Chosun society. In 'Sil-Hak (the Practical Learning) period' which began in the late 16th century, especially in the reigns of Kings Youngjo and Jungjo, which was called the Renaissance of Chosun, the ambitious policy for the development of science and technology called for. the rapid increase of he number of such technocrats as mathematics, astronomy and medicine. Amid these social changes, the Jung-in mathematicians inevitably became quite ambitious and proud. They tried to explore deeply into mathematics perse beyond the narrow limit of knowledge required for their office. Thus, in this period the mathematics developed rapidly, undergoing very important changes. The characteristic features of the mathematics in this period were: Jung-in mathematicians' active study an publication, the mathematic studies by the renowned scholars of Sil-Hak, joint works by these two classes, their approach to the Western mathematics and their effort to develop Korean mathematics. Toward the 'Enlightenment Period' in the late 19th century, the Western mathematics experienced great difficulty to take its roots in the Peninsula which had been under the strong influence of Confucian ideology and traditional Korean mathematic system. However, with King Kojong's ordinance in 1895, the traditional Korean mathematics influenced by Chinese disappeared from the history of Korean mathematics, as the school system was hanged into the Western style and the Western mathematics was adopted as the only mathematics to be taught at the Schools of various levels. Thus the 'Enlightenment Period' is the period in which Korean mathematics shifted from Chinese into European.

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족삼리(足三里) 배혈(配穴)에 따른 전침(電鍼)이 흰쥐 대뇌피질(大腦皮質)의 NADPH-diaphorase와 nNOS, NPY, VIP 신경세포(神經細胞)에 미치는 영향(影響) (Effect of Joksamni combination on NADPH-diaphorase, neuronal Nitric Oxide Synthase, Neuropeptide Y and Vasoactive Intestinal Peptide in the cerebral cortex of Spontaneously Hypertensive Rat)

  • 정인기;이재동;김창환
    • Journal of Acupuncture Research
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    • 제20권5호
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    • pp.118-132
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    • 2003
  • Objective: The aim of this study was to investigate the effects of Joksamni(ST36) combination on NAD PH-diaphorase, neuronal nitric oxide synthase(nNOS), neuropeptide Y(NPY) and vasoactive intestinal peptide (VIP) in the cerebral cortex of spontaneously hypertensive rat. Methods: The experimental groups were divided into four groups: Normal, Joksamni(ST36), Joksamni(ST36)+Eumneungcheon(SP9), and Joksamni(ST36)+Gokji(LI11). Needles were inserted into acupoints at the depth of 0.5cm with basic insertion method. Electroacupuncture was done under the condition of 2Hz electrical biphasic pulses with continuous rectangular wave lasting for 0.2ms until the muscles produced visible contractions. Such stimulation was applied continuously for 10 minutes, 1 time every 2 days for 10 sessions of treatments. Thereafter we evaluated changes in NADPH-d positive neurons histochemically and changes in nNOS, NPY and VIP positive neurons immunohistochemically. Results: The optical densities of NADPH-d positive neurons of the Joksamni(ST36)+Eumneungcheon(SP9) group in all areas of cerebral cortex and Joksamni(ST36)+Gokji(LI11) group in primary somatosensory cortex, visual cortex, auditory cortex, perirhinal cortex were significantly increased as compared to the Joksamni(ST36) group. The optical densities of NADPH-d positive neurons of the Joksamni(ST36)+Gokji(LI11) group were significantly decreased as compared to the Joksamni(ST36)+Eumneungcheon(SP9) group with the exception of primary somatosensory cortex. The optical densities of nNOS positive neurons of the Joksamni(ST36)+Eumneungcheon(SP9) group in all areas of cerebral cortex and Joksamni(ST36)+Gokji(LI11) group in auditory cortex, perirhinal cortex, insular cortex were significantly increased as compared to the Joksamni(ST36) group. The optical densities of nNOS positive neurons of the Joksamni(ST36)+Gokji(LI11) group were significantly decreased in all areas of cerebral cortex as compared to the Joksamni(ST36)+Eumneungcheon(SP9) group. The optical densities of NPY positive neurons of the Joksamni(ST36)+Gokji(LI11) group were significantly decreased in primary motor cortex, primary somatosensory cortex, cingulate cortex as compared to the Joksamni (ST36) and Joksamni(ST36)+Eumneungcheon(SP9) groups. The optical densities of VIP positive neurons of the Joksamni(ST36)+Eumneungcheon(SP9) group were significantly increased in all areas of cerebral cortex except for cingulate cortex as compared to the Joksamni(ST36) group. The optical densities of VIP positive neurons of the Joksamni(ST36)+Gokji(LI11) group were significantly decreased in auditory cortex, cingulate cortex, perirhinal cortex as compared to the Joksamni(ST36) group. The optical densities of VIP positive neurons of the Joksamni(ST36)+Gokji(LI11) group were significantly decreased in all areas of cerebral cortex as compared to the Joksamni(ST36)+Eumneungcheon(SP9) group. Conclusions: The result demonstrated that electroacupuncture on Joksamni(ST36) and its combination change the activities of the NO system and peptidergic system in the cerebral cortex of SHR and that acupoint combination is one of the important parameters for the effects.

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한국 약용식물 추출물이 In vitro 돼지 지방조직의 지방합성과 지방전구세포의 분화에 영향을 미친다 (Extracts of Korean Medicinal Plant Extracts Alter Lipogenesis of Pig Adipose Tissue and Differentiation of Pig Preadipocytes In vitro)

  • 최영숙;최강덕;김성도;필립 오웬즈;정정수
    • Journal of Animal Science and Technology
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    • 제52권5호
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    • pp.383-388
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    • 2010
  • In vivo 실험에 의해서 지방축적을 억제할 수 있는 물질을 찾아내는 것은 시간과 비용이 많이 든다. 본 연구는 돼지 지방조직을 이용한 in vitro system을 이용해서 한국의 약용식물 중에서 지방축적 억제 작용을 가지는 것을 조사하고자 수행하였다. 총 183종의 약용식물을 이용하여 이들이 돼지 지방조직의 지방합성과 지방전구세포의 분화에 미치는 작용을 조사하였다. 에탄올 추출물은 72종, 물 추출물은 111종, 이 중에서 65종은 물과 에탄올 모두 이용해서 추출하였다. 돼지 지방조직의 지방합성은 13종류의 약용식물이 영향을 미쳤는데, 그 중 11종은 대조구에 비하여 지방합성을 40% 이상 억제하였고, 4종은 지방합성을 70% 이상 억제하였다. 가장 강력한 지방합성 억제작용을 나타낸 것은 붓꽃과와 고삼(에탄올 추출) 그리고 좁쌀풀(물과 에탄올 추출)이었다. 그러나 소목과 황백은 지방합성을 촉진하였다. 돼지 지방전구세포의 분화에 미치는 결과는 총 28종의 약용식물이 돼지 지방전구세포의 분화에 영향을 미쳤는데, 이 중 16종의 약용식물은 증가시켰고, 12종은 억제시켰다. 목단피와 강활(에탄올 추출) 그리고 당귀, 목향 및 신이(물 추출)는 지방세포의 분화를 두 배 정도 증가시켰다. 10종의 약용식물 즉 감초, 형개 및 구월나무(에탄올 추출) 그리고 비름과, 천문동, 백출, 유자나무, 향부자, 구엽초 및 목단피(물 추출)은 지방세포의 분화를 35% 이상 억제시켰다. 구월나무(에탄올 추출)만이 지방합성뿐만 아니라 세포분화도 억제하였다. 본 연구 결과는 약용식물로부터 가축과 사람의 지방축적 억제효능 후보물질을 발굴하기 위한 기초자료로 활용할 수 있을 것으로 여겨진다.

한우 수정란의 산소 소비량이 수청란이식 수태윷에 미치는 영향 (Effects of Pregnant Rate after Embryo Transfer in Oxygen Consumption of Embryos in Korean Cattle)

  • 최창용;손준규;조상래;강다원;연성흠;최선호;최수호;김남태;김재범;정연섭;김성재;정진우;복난희;유용희;손동수
    • 한국수정란이식학회지
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    • 제25권3호
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    • pp.145-148
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    • 2010
  • Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

Reduced Glutathione의 In Vitro 첨가(添加)가 마우스간조직(肝組織)의 내재(內在) NP-SH 및 NP-SS에 미치는 영향(影響) (Effect of Reduced Glutathione on Non-Protein Sulfhydryl and Non-Protein Disulfide of the Mouse Liver in Vitro)

  • 오상유
    • The Korean Journal of Physiology
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    • 제7권2호
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    • pp.9-16
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    • 1973
  • 마우스 간조직(肝組織)을 수종(數種) 농도(濃度)의 GSH 용액중(溶液中)에서 각각(各各) 4 C, 25 C 및 37 C의 온도(溫度)속에서 incubate하고, 5분(分), 30분(分) 및 60분(分)에서 각각(各各) NP-SH 및 NP-SS의 양(量)을 정량(定量)하여 정상(正常) 및 대조군(對照群)의 그것들과 비교(比較)한 결과(結果) 다음의 결과(結果)를 얻었다. 1) 4 C에서 incubate한 군(群)에서는 대조군(對照群) 및 GSH 10 gm/100 ml KRP의 농도(濃度)에서 대체(大體)로 정상치(正常値)에 비(比)하여 대차(大差)없는 NP-SH 및 NP-SS를 나타내고 GSH의 농도(濃度)가 높아져도 SH 기(基)의 증가(增加)는 현저(顯著)치 않았다. 2) 25 C에서 incubate한 군(群)에서의 NP-SH및 NP-SS는 GSH 20 mg/100 mg KRP 및 GSH 30 mg/100 mg KRP 의 양군(兩群)에서 GSH의 농도(濃度)와 시간경과(時間經過)에 비례(比例)해서 증가(增加)하였고, GSH 10 mg/100 ml KRP 군(群)에서는 incubation 30분(分)에서 최고치(最高値)를 나타내고 60분(分)에서는 오히려 약간(若干) 저하(低下)되는 경향(傾向)을 나타내었다. 3) 37 C에서 incubate 한 군(群)에서는 GSH 농도(濃度)와 incubation의 시간(時間)에 비례(比例)해서 NP-SH 및 NP-SS가 대체(大體)로 증가(增加)하고 증가(增加)의 율(率)도 가장 현저(顯著)하였다.

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사고사례를 통한 재가치매환자의 위험요소 추출 및 그 활용에 관한 연구 (A study on the extraction of risk factor and its application for senile dementia patient at home based on accidental cases)

  • 이행운;엄진섭;최미현;이수정;최진승;문석우;탁계래;정순철
    • 감성과학
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    • 제12권1호
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    • pp.11-18
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    • 2009
  • 본 연구의 목적은 재가치매노인에게 발생한 사고사례들의 조사를 통해 치매노인에게 위험요소로 작용 될 수 있는 요인들을 추출하고, 이를 바탕으로 치매노인 보호를 위해 고려해야 할 모니터링 요소의 결정에 필요한 기초 정보를 제공하고자 하는 것이다. 재가치매노인 55명(남자 16명, 여자 39명)을 대상으로 설문지를 이용하여 기본특성, 행동특성, 노인인지기능(Short form of Samsung Dementia Questionnaire: S-SDQ), 일상생활 수행능력(Activities of Daily Living: ADL), 사고사례를 조사하였다. 설문조사내용을 바탕으로 위험인자로 작용하는 요소들을 추출하고, 추출된 요소들의 발생빈도, 공발생빈도, 발생장소, 신체손상유무, 손상부위, 손상정도를 분석하였다. 그리고 행동특성, ADL, S-SDQ와 위험요소간의 발생빈도를 교차빈도분석을 통해 알아보았다. 분석결과 12개의 위험요소가 추출되었으며, 이 중 '나감'의 빈도가 가장 많았고, 신체적 손상이 발생한 위험요소는 넘어짐, 부딪힘, 미끄러짐, 떨어짐이었다. 공발생빈도 분석 결과, 넘어짐, 나감, 가스방화, 폭력이 다른 요인과 함께 발생하는 빈도가 높았다. 위험요소의 발생장소는 집주변이 가장 많았으며, 손상부위로는 무릎, 손상정도는 타박상이 가장 많았다. 교차빈도분석결과 위험요소의 발생빈도에 차이가 발생한 항목은 행동장애, 일상생활수행장애 그리고 ADL이였다. 행동장애와 일상생활수행장애의 정도에 따라 빈도의 차이를 보인 요인은 나감이었고, ADL의 정도에 따라 빈도의 차이를 보인 요인은 미끄러짐과 가스방화였다.

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혈소판유래성장인자를 함유한 Chitosan/Calcium Metaphosphate의 골조직재생효과에 관한 연구 (The Effect of Platelet Derived Growth Factor - BB Loaded Chitosan/Calcium Metaphosphate on Bone Regeneration)

  • 이승열;설양조;이용무;이주연;이승진;김석영;구영;류인철;한수부;최상묵;정종평
    • Journal of Periodontal and Implant Science
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    • 제31권1호
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    • pp.1-23
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    • 2001
  • Chitosan is biodegradable natural polymer that has been demonstrated its ability to improve wound healing, and calcium metaphosphate(CMP) is a unique class of phosphate minerals having a polymeric structure. In this study, chitosan/CMP and platelet derived growth factor(PDGF-BB) loaded chitosan/CMP sponges were developed, and the effect of the sponges on bone regeneration and their possibility as scaffolds for bone formation by three-dimensional osteoblast culture were examined. PDGF-BB loaded chitosan/CMP sponges were prepared by freeze-drying of a mixture of chitosan solution and CMP powder, and soaking in a PDGF-BB solution. Fabricated sponge retained its 3-dimensional porous structure with $100-200\;{\mu}m$ pores. The release kinetics of PDGF-BB loaded onto the sponge were measured in vitro with $^{125}I-labeled$ PDGF-BB. In order to examine their possibility as scaffolds for bone formation, fetal rat calvarial osteoblastic cells were isolated, cultured, and seeded into the sponges. The cell-sponge constructs were cultured for 28 days. Cell proliferation, alkaline phosphatase activity were measured at 1, 7, 14 and 28 days, and histologic examination was performed. In order to examine the effect on the healing of bone defect, the sponges were implanted into rat calvarial defects. Rats were sacrificed 2 and 4 weeks after implantation and histologic and histomorphometrical examination were performed. An effective therapeutic concentration of PDGF-BB following a high initial burst release was maintained throughout the examination period. PDGF-BB loaded chitosan/CMP sponges supported the proliferation of seeded osteoblastic cells as well as their differentiation as indicated by high alkaline phosphatase activities. Histologic findings indicated that seeded osteoblastic cells well attached to sponge matrices and proliferated in a multi-layer fashion. In the experiments of implantation in rat calvarial defects, histologic and histomorphometric examination revealed that chitosan/CMP sponge promoted osseous healing as compared to controls. PDGF-BB loaded chitosan/CMP sponge further echanced bone regeneration. These results suggested that PDGF-BB loaded chitosan/CMP sponge was a feasable scaffolding material to grow osteoblast in a three-dimentional structure for transplantation into a site for bone regeneration.

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돌가자미 Kareius bicoloratus의 정자형성과정 중 생식세포, Cyst 상피세포 및 간질세포의 미세구조 (Ultrastructure of Germ Cells, Cyst Epithelial Cells and Interstitial Cells during Spermatogenesis of the Stone Flounder, Kareius bicoloratus)

  • 전제천;정의영;양영철
    • 한국어류학회지
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    • 제18권4호
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    • pp.311-318
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    • 2006
  • 한국 서해안에서 채집한 돌가자미 Kareius bicoloatus의 정자형성과정 중 생식세포, Cyst 상피세포 및 간질세포들의 미세구조를 전자현미경 관찰에 의해 조사하였다. 제1차 정모세포에서 연접사복합체(synaptonemal complex)들이 성숙분열 중 전기의 쌍사기에 출현하였다. 특히, 감수분열을 위한 테스토스테론 호르몬을 분비하는 세포로 알려진 간질세포(Leydig cell)들은 제1차 정모세포 및 정세포 가까이에서 뚜렷하게 출현하였다. 포상핵을 가지는 이들 간질세포들은 소엽 간 간질조직 내의 섬유모세포들에 의해 둘러싸여 출현하였다. 특히, 이들 세포들은 타원형 또는 구상의 미토콘드리아들을 가지는데, 미토콘드리아들은 관상 크리스테(cristae)를 갖는 것이 특징이다. 또한, 활면소포체와 여러 개의 공포들도 세포질 내에서 출현하였다. 성장 발달 중인 정소 내에서 정소소엽 간 간질조직에서 나타나는 잘 발달된 간질세포들(스테로이드호르몬분비세포)은 포상의 핵과 관상 크리스테를 갖는 미토콘드리아를 가지며, 활면소체를 갖는 형태적 특징을 보였다. 정자형성과정 중, 제1차, 제2차 정모세포들은 cyst 상피세포 (Sertoli cell)들에 인접하거나 부착하여 나타났다. cyst 상피세포들의 핵은 길게 신장된 타원형 또는 삼각형의 핵을 가지며 세포질 내에 여러 개의 미토콘드리아가 출현하였다. 성장 발달 중인 정소 내에서 제2차 정모세포들과 정세포 가까이에 위치한 cyst 상피세포의 세포질 내에는 지방적, 미토콘드리아 로제트(mitochondeial rosettes)들과 글리코겐 입자들이 나타났다. 특히, 후기 성장 중인 정소 내에서 cyst 상피세포의 세포질 내에는 미토콘드리아, 소포체, 작은 지방적 및 다량의 글리코겐입자들이 출현하였다. 정세포 발달(정자변태) 후기에 기부중심립이 핵막에 부착하며, 원위중심립은 편모의 기저체를 형성하고, 편모축사를 형성하였다. 정자 첨체는 다른 경골어류에서와 같이 첨체를 형성하지 않았다. 정자 두부의 길이는 대략 $3{\mu}m$ 정도이며, 미부길이는 약 $30{\mu}m$ 정도이다. 정자 미부편모의 축사(axoneme)는 주변에 9쌍의 주변이중미세관 (nine outer doublet microtubule)이 있고 중앙에 두 개의 중앙미세관(two centrial singlet microtubules)으로 구성되어 9+2구조를 나타내었다. 본 종의 정자는 체외수정 종들이 갖는 두 개의 axonemal lateral fin을 가진다. 본 연구에서 여러 단계의 생식세포들 가까이에서 출현하는 cyst 상피세포(Sertoli cell)들은 영양공급, 식세포작용, 스테로이드형성의 3가지 기능을 나타내었다. 특히, 정소 발달단계 중 퇴화 회복기에 출현하는 cyst 상피세포들의 핵은 배정 후에는 모양이 불규칙하게 나타나며, 식세포작용을 나타내는 여러 특징들이 cyst 상피세포의 세포질 내에서 나타나고 있어, cyst 상피세포들은 배정 후 방출되지 않은 생식세포들의 퇴화 흡수에 관여하는 것으로 추정된다.