• BMB Reports
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• v.39 no.5
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• pp.626-635
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• 2006

Lysophosphatidic acid acyltransferase (LPAAT) is an intrinsic membrane protein that catalyzes the synthesis of phosphatidic acid (PA) from lysophosphatidic acid (LPA). It is well known that LPAAT is involved in lipid biosynthesis, while its role in tumour progression has been of emerging interest in the last few years. To date, seven members of the LPAAT gene family have been found in human. Here we report a novel LPAAT member, designated as LPAAT-theta, which was 2728 base pairs in length and contained an open reading frame (ORF) encoding 434 amino acids. The LPAAT-theta gene consisted of 12 exons and 11 introns, and mapped to chromosome 4q21.23. LPAAT-theta was ubiquitously expressed in 18 human tissues by RT-PCR analysis. Subcellular localization of LPAAT-theta-EGFP fusion protein revealed that LPAAT-theta was distributed primarily in the endoplasmic reticulum (ER) of COS-7 cells. Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells.

• Molecules and Cells
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• v.19 no.1
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• pp.39-45
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• 2005

RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.

• BMB Reports
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• v.37 no.4
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• pp.402-407
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• 2004

A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.

• Korean Journal of Health Education and Promotion
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• v.19 no.3
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• pp.121-133
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• 2002

The purpose of this study was to set up the basic model to monitor the health status of the middle and high school students in the adolescent period, as well as to provide the fundamental data which might serve as the new standards for their better health maintenance and promotion. The collected data comprised the characteristics of the subjects in the three different fields of life such as school, family and everyday, which could affect their physical fatigues, and they were analyzed to determine the factors of significant influence upon their physical fatigues and to reveal their relativity. A total of 5,954 students at 6 boys' and girls' middle and high schools in Taejon City completed self-administered questionnaires(to measure Health Practice Index and others) asking about each of three life domains during the period March through June, 2000. The key study results were as follows; 1. The middle school students showed more subjective symptoms of fatigue which may be a marker reflecting physical health status, than the high school students. The higher scores were associated, based on school life, with the groups who were boys, who were in lower school grades, who achieved better academic performances and had better relationship with friends. 2. As for family life, the higher scores were in the groups whose financial conditions were better, whose parents showed more interest to their children, whose sense of satisfaction with family life was higher, and whose health status was better. 3. As for life styles, the individuals showed the higher scores in the groups who had breakfasts daily, who didn't have inter-meal snacks, who took regular exercises, who didn't smoke and drink than, their counterparts. 4. The higher level of fatigue was associated with the groups whose health conditions were good, who are satisfied with school life, who were boys rather than girls, who are satisfied with family life, who didn't have inter-meal snacks, who were in good relationship with friends, who achieved greater academic performances, who took regular exercises, who didn't smoke, and whose parents showed more interest in their children.

• Journal of Veterinary Science
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• v.22 no.1
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• pp.8.1-8.11
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• 2021

Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

• Imaging Science in Dentistry
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• v.51 no.1
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• pp.73-80
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• 2021

Purpose: The purpose of this study was to evaluate the cone-beam computed tomographic (CBCT) imaging and histopathological characteristics of osteoradionecrosis(ORN) and medication-related osteonecrosis of the jaw(MRONJ). Materials and Methods: Ten surgical specimens from segmental mandibulectomy (3 ORN and 7 MRONJ) were analyzed using CBCT. The CBCT parameters were as follows: high-resolution mode (tube voltage, 90.0 kV; tube current, 4.00 mA; rotation time, 16.8 s; field of view, 56 mm×56 mm; thickness, 0.099 mm). Histopathological characteristics were evaluated using histological slides of the surgical specimens. The Pearson chi-square test was used to compare ORN and MRONJ in terms of CBCT findings(internal texture, sequestrum, periosteal reaction and cortical perforation) and histopathological characteristics(necrotic bone, inflammatory cells, reactive bone formation, bacteria, Actinomyces, and osteoclasts). A P value less than 0.05 was considered to indicate statistical significance. Results: MRONJ showed periosteal reaction on CBCT more frequently than ORN (7 of 7 [100%] vs. 0 of 3 [0%], P<0.05). Regarding histopathological characteristics, MRONJ showed osteoclasts more frequently than ORN (6 of 7 [85.7%] vs. 0 of 3 [0%], P<0.05). Conclusion: This study evaluated the CBCT imaging and histopathological characteristics of ORN and MRONJ, and the findings suggest that CBCT could be useful for the evaluation of ORN and MRONJ.

• Journal of the Korean Home Economics Association
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• v.43 no.2
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• pp.203-229
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• 2005

The purpose of this study was to explore the perceived quality of life of Korean university students'. The perceived quality of life was composed of three dimensions: emotion, cognitive efficiency, and satisfaction. The study results were as follow: 1. Korean university students felt more positive emotions when they spent time for social activities. 2. In the cognitive efficiency, social activities also scored highly of 5 questions, but in the item of "awareness of another person's eye", productive activities were higher. 3. Korean university students were more satisfied with themselves when they spent time for productive activities. However, they were more satisfied with their work itself when the work was social activities. 4. Korean university students' emotions, cognitive efficiencies, and satisfaction were significantly different by socio-graphic variables and context.

• Journal of Families and Better Life
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• v.31 no.1
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• pp.39-48
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• 2013

This study examined the variables that influence middle school students' level of life satisfaction and tested a hypothetical structural equation model relating the variables to middle school students' level of life satisfaction. The subjects were 2,829 (1,411 boys and 1,418 girls) pupils who participated in the Korean Survey on the Familial Consciousness of Youth and Parents (KSYC-Familial Consciousness) in 2010. Data were analyzed through frequency, percentages, Pearson's correlations using SPSS 18.0 and structural equation modeling with AMOS 7.0. The findings are as follows. The proposed model, as revised, demonstrated the effectiveness of an analysis of the structural equation model and illustrated that all three variables (fathers' parenting behavior, communication with fathers, self-concept) indicated a direct or indirect influence on middle school students' level of life satisfaction. Fathers' parenting behavior had the greatest total effect on middle school students' life satisfaction. Fathers' parenting behavior, communication with fathers and self-concept explained 59% of the total variance of middle school students' life satisfaction. The implications for future studies are also discussed.

• Child Health Nursing Research
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• v.16 no.3
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• pp.220-229
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• 2010

Purpose: The purpose of this study was to describe quality of life in school age children and the relationship between quality of life and parenting attitude as perceived by children and parent-child communication pattern. Methods: Data were collected between May 26 and June 3, 2009 using self-report questionnaires from 148 pairs of children and their parents. The children were enrolled in 4th to 6th grades. The questionnaires included the Parental Bonding Instrument (PBI), Parent-Child Communication (PCC) Scale, and Pediatric Quality of Life Inventory (PedsQL). The data were analyzed using the SPSS WIN 17.0 program. Results: The Quality of Life (QOL) score for the children was significantly higher among children reporting higher parental bonding attitude (mother r=.38, father r=.34) and parent-child communication (mother r=.43, father r=.36). Results of multiple regression showed that factors significantly associated with QOL were academic achievement, caregiver available after school, parent-child communication, school satisfaction, relationship with peers, and birth order among siblings. Conclusion: Positive relationships and good communication with parents and satisfaction with school life were important factors associated with quality of life in school age children.

• Journal of Family Resource Management and Policy Review
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• v.12 no.3
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• pp.103-117
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• 2008

This study focuses on time management behavior, which differs in (1) the characteristics of schools and (2) the experience of education about time management. Also, this study examines the effect of time management behavior on life satisfaction of vocational and non-vocational high school students, and also those who attend life-long education schools. The study sample consists of 367 teen-age high school students in Seoul. The result of this study demonstrates that the experience of education about time management has a great effect on time management behavior. Students experiencing education about time management have higher scores in time management behavior than those who lack such experience. It also shows a close relationship between time management behavior and life satisfaction in non-vocational high school students and those attending life-long education schools.