• 제목/요약/키워드: scarification

검색결과 36건 처리시간 0.025초

Germination of West African Ebony (Diospyros mespiliformis Hochst) Seeds: Effects of Dehydration and Different Pre-sowing Treatments

  • Kanmegne, Gabriel;Mbakop, Christelle Nya;Fonkou, Theophile
    • Journal of Forest and Environmental Science
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    • 제36권4호
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    • pp.267-273
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    • 2020
  • Diospyros mespiliformis is a highly valued and threatened tree species within the Sahelo-Sudanian zone of Africa, but its seed germination requirements under cultivation are not well researched. In a first experiment which aimed at determining germination response of seeds to dehydration, fresh seeds were dried at room temperature for 26 days during which their moisture content, their germinability, and their viability were monitored at two-day intervals. In the second experiment, 14 pre-germination treatments were tested for their effect on the germination of dried seeds. Results showed that fresh seeds had 52.7% moisture and achieved 97.7% germination. As seeds were dried, percentage germination gradually decreased with decreasing moisture content and reached 0% when moisture content had dropped to 18%. Meanwhile, seed viability remained at 100% over drying duration. Seeds that were not germinated after air dry also recorded 100% viability. The most effective treatment for inducing germination of dried seeds was scarification using 98% sulfuric acid for 30 min which resulted in 96.6% germination. This study reports for the first time in D. mespiliformis seeds a desiccation-induced dormancy which can be efficiently alleviated by acid scarification. This study provides useful information that will contribute to efficient management of D. mespiliformis seed resources for propagation.

파상처리와 지베렐린을 이용한 무늬둥굴레(Polygonatum odoratum var. pluriflorum 'Variegatum')의 휴면타파 (Scarification and Gibberellic Acid Affecting to Dormancy Breaking of Variegated Solomon's Seal (Polygonatum odoratum var. pluriflorum 'Variegatum'))

  • 이용하;이승연;박주현;김기선
    • 원예과학기술지
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    • 제32권3호
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    • pp.296-302
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    • 2014
  • 무늬 둥굴레는 화훼장식의 절엽 소재로 많이 활용되고 있으나 봄과 여름 시기에만 생산되는 제한적인 시장성을 가지고 있다. 무늬 둥굴레의 연중생산 요구가 늘어나고 있으며, 시간과 공간을 필요로 하는 저온처리를 대신하는 무늬둥굴레의 휴면타파법이 필요하다. 본 연구에서는 지베렐린을 효과적으로 처리하여 무늬둥굴레의 휴면을 타파할 수 있는 방법을 찾고자 실험을 수행하였다. 휴면 상태의 무늬둥굴레 근경에 지베렐린을 두상관주 하였을 때에는 휴면타파가 이루어 지지않았다. 하지만 지베렐린을 처리하기 전에 면도칼로 무늬둥굴레의 표피에 상처를 내어 지베렐린을 관주하거나 주사로 지베렐린을 직접 주입하였을 때에는 1년차 실험에서는 $GA_3$ $400mg{\cdot}L^{-1}$를 처리했을 때 각각 100, 83.3%의 휴면타파가 이루어졌다. 그러나 면도날이나 주사기를 사용했을 경우 무늬둥굴레에 물리적인 피해가 나타나 NaOCl을 이용한 화학적 처리를 사용해 보았다. 솜에 묻힌 4%의 NaOCl을 6시간 또는 24시간 처리한 이후에 $GA_3$ $400mg{\cdot}L^{-1}$를 처리했을 때 휴면 상태의 무늬둥굴레에서 각각 70%와 86.7%의 휴면타파 결과를 얻을 수 있었다. 그리고 대조구나 지베렐린의 관주처리에 비하여 물리적인 처리 이후 지베렐린을 처리했을 때 엽수가 더 많았다. 이와 같은 결과는 지베렐린을 효과적으로 처리하면 무늬둥굴레의 휴면을 효율적으로 타파시켜서 절엽의 주년생산에 기여할 수 있음을 알 수 있었다.

섬바디의 재배상 문제점과 해결방안 (Problems and Their Solutions in Growing Seombadi ( Dystaenia take simana ))

  • 허삼남
    • 한국초지조사료학회지
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    • 제2권2호
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    • pp.16-20
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    • 1981
  • To cultivate Dystaenia takesimana as a fodder crop, Its problems and solutions are summarized follows : 1. There are a wax layer and wings on the out side of seed coat of D. takesimana seeds, which seems to inhibit imbibition and cause delayed germination. 2. The root and shoot growth of D. takesimana are very poor in the early stage and weed control is quite troublesome. 3 The growth of D. takesimana is very poor the soil of low fertility and much depressed in summer. 4 . The percent germination could be increased by removing wings, soaking, high temperature treatment or by chemical scarification. 5. Compost is most effective for the growth of D. takesimana and lime dressing is desired in the soil of low pH. 6 . The seedling growth could be improved selecting and breeding the strains with better germination and root growth, at the germination and growth characteristics of D. takesimana are variable.

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New Fabrication Approach for Low Voltage Driving Electrophoretic Display

  • Park, Young-Mi;Kim, Do-Yun;Cho, Young-Tae;Lee, Yong-Eui;Kim, Chul-Hwan;Yoon, Sang-Soo
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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    • pp.893-895
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    • 2009
  • In this paper, we described the unique and novel method to prepare two kinds of electro-active particles, black and white particles with different polarity. The surface of the particles was characterized to be uniform and clean by adopting spray dryer as a tool for genesis of particles; neither surfactants nor high dielectric medium like water was employed during particle preparation step. The other purpose of this study is to investigate the factors that contribute high driving voltage of particlebased display like QR-LPD. We extracted parameters interaction between particle and electrode, and between oppositely charged particles. Here we reported an excellent behavior of particle-based display that showed low operating voltage, high contrast ratio as high as 8:1 without scarification of quick response time. By optimizing the particle size, charge per mass, selection of external additive sets, a lower driving voltage as low as 40V for the particles with $10{\mu}m$ volume average diameter was obtained.

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황산처리를 이용한 개싸리 종자의 물리적 휴면 타파 (Breaking Physical Dormancy with Sulfuric Acid in Seeds of Lespedeza tomentosa (Thunb.) Siebold ex Maxim)

  • 이용하;최한;이수광;이정호;이기철
    • 한국자원식물학회지
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    • 제29권1호
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    • pp.136-142
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    • 2016
  • 싸리속(Lespedeza)식물은 가축사료, 제방 녹화용 등으로 활용되는 자원식물이다. 이들 종자는 산지의 화재 이후에 발아, 출현하는 fire species로 알려져 있다. 선행연구에서 이런 점을 착안하여 인위적으로 열을 가했을 때 발아가 되었으나 발아율이 낮고 처리가 용이하지 않았다. 따라서 본 연구는 개싸리 종자를 황산처리하여 화학적으로 종피처리하여 발아율을 높이기 위해 수행되었다. 개싸리 종자를 98% 황산에 0, 1, 3, 6, 12, 24, 48, 96, 192, 384분 동안 침지시키고 흐르는 물에 24시간 동안 씻었다. 황산처리를 하지 않은 대조구의 경우 거의 발아가 되지 않았지만, 황산처리 기간이 길어질수록 개싸리의 발아율이 증가하였다. 황산 24, 48, 92분 처리에서 90% 이상 발아가 되었다. 그러나 장기간 황산처리시 발아된 유근에 황산피해가 관찰되었다. 기외 실험에서 황산처리 후 건전묘 출현을 알아보기 위해 황산 30, 60, 90, 120, 150, 180, 300분을 처리한 원예상토가 충진된 트레이에 파종하고 비닐온실에서 발아율을 측정하였다. 황산 90분, 150분 처리에서 발아율은 각각 92%, 84%였으나 이보다 더 긴 기간 동안 처리하였을 때 출현율이 떨어졌다. 따라서 개싸리의 발아를 위한 황산 최적시간은 1~2시간으로 판단된다. 개싸리는 종피처리 이후 바로 발아가 시작되어 대부분 1달 이내에 발아가 완료되었으며, 이는 개싸리 종자는 생리적 휴면은 없는 물리적 휴면을 가지고 있음을 알 수 있다.

$GA_3$, Kinetin 및 물리적 처리가 초피나무 종자의 발아에 미치는 영향 (Effect of $GA_3$, Kinetin and Physical Treatment on the Seed Germination of Zanthoxylum piperitum A.P. DC.)

  • 김세종;신종희;김기재;박소득;최부술;김길웅
    • 한국약용작물학회지
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    • 제5권1호
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    • pp.43-48
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    • 1997
  • 천연향신료와 식용 및 약용 겸용으로 재배가치가 매우 높은 초피나무종자의 발아율 향상을 위하여 $GA_3$, Kinetin 및 물리적인 처리를 하여 수행한 실험 결과를 요약하면 다음과 같다. 1. 초피 나무종자 종피파상후 $GA_3$ 50 ppm 24시간 침지에서 발아율 8.9%로 저조하였다. 2. 수온 $18^{\circ}C\;1{\sim}5$일 및 $40{\sim}70^{\circ}C$에서 10분처리 후 각각 $GA_3$ 50 ppm에 24시간 침지에서 초피종자 발아율 6.8, 4.4%로 매우 낮았다. 3. 화학적인 방법으로 $H_2SO_4,\;NaOH$$HNO_3$ 침지에서 초피나무종자 발아율 7,5, 2.7, 14.3%로서 저조하였다. 4. 초피나무종자에 대한 $GA_3$처리의 경우 $GA_3$ 100 ppm에서 48시간 침지가 91.1%의 가장 높은 발아율을 나타내었고 침지시간이 길어질수록 농도가 높을수록 발아율이 높아지는 경향이었다. 5. Kinetin은 50 ppm 12시간 침지에서 초피종자의 발아율은 31.7%로 양호하였으나 전반적으로 낮은 발아율을 나타내어 실용적인 방법으로는 미흡하였다.

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Gene Expression Profiling of 6-MP (6-mercaptopurine) in Liver

  • Kim Hyung-Lae;Kim Han-Na;Lee Eun-Ju
    • Genomics & Informatics
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    • 제4권1호
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    • pp.16-22
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    • 2006
  • The KFDA (Korea Food & Drug Administration) has performed a collaborative toxicogenomics project since 2003. Its aim is to construct a toxicology database of 12 compounds administered to mice at initial phase. We chose 6-MP (6-mercaptopurine) which has been used in the treatment of childhood leukemia. It was administered at low (0.224 mg/kg) and at high (2.24 mg/kg) dose (5 mice per group) intraperitonealy to the postnatal 6 weeks mice, then the serum and liver were collected at the indicated time (6, 24 and 72 h) after scarification. Serum biochemical markers for liver toxicity were measured and histopathologic studies also were carried out. The gene expression profiling was carried out by using Applied Biosystems 1700 Full Genome Expression Mouse. By self-organization maps (SOM), we identified groups with unique gene expression patterns, some of them are supposed to be related to 6-MP induced toxicity, including lipid metabolism abnormality, inflammatory response, oxidative stress, ATP depletion and cell death. The potential toxic effects appearing as gene expression changes are dependent of the time of 6-MP but independent of the dosage of it. This study would contribute to establishment of international database as well as national one about hepatotoxicity.

결막접근을 통한 근막초 고정법(capsulopalpebral fascia fixation)을 이용한 안와지방 돌출의 교정 (Transconjunctival capsulopalpebral fascia fixation for the correction of orbital fat buldging)

  • 이은정
    • Archives of Plastic Surgery
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    • 제36권2호
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    • pp.194-199
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    • 2009
  • Purpose: The orbital fat buldging may cause irregular contour of lower eyelid, which is not youthful appearance. The conventional method of fat excision may cause the eyeball to move backward and downward, making enopthalmia is inevitable. The transcutaneous approach to reach the orbital septum can increase the risks of scleral show resulting from scarification at the level of the septum orbicularis junction and damaging the vertical motor branches of the preseptal orbicularis oculi muscle of the lower eyelid. Method: From July 2007 to March 2008, total of 21 patients underwent transconjunctival capsulopapebral fascia fixation procedure. In 8 patients, the herniated fat pad of the lower eyelid was relocated back into the orbit without external skin excision using capsulopalpebral fascia fixation. But in 13 patients, excision of redundant skin of the lower eyelid was performed using pinching technique, not involving orbicularis oculi muslce. In 5 patients, lateral canthotomy was done bilaterally for good visual field. In 6 patients, lateral canthopexy was also combined to correct loosening of lower eyelid. Results: Most of the patients were satisfied after at least 5 months of follow up. No patient showed scleral show. But 2 patients complained of undercorrection slightly, without secondary operation in 1 patient and re - capsulopalpebral fascia fixtation through transconjunctival approach in another patient. Conclusion: Orbital fat repositioning using transconjunctival capsulopalpebral fascia fixation is a good procedure to show youthful appearance without increasing the risk of scleral show and also showed fast recovery compared to the conventional transcutaneous approach.

Low Voltage and Rapid Response Time Electrophoretic Display

  • Lee, Y.E.;Cho, Y.T.;Choi, Y.G.;Park, S.C.;Lee, M.H.;Park, Y.M.;Kim, D.Y.;Kim, C.H.;An, C.H.;Kim, H.S.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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    • pp.360-363
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    • 2009
  • In this paper, we describe new approach of ink particle fabrication method for electrophoretic display(EPD) with low voltage and rapid response time. Nano-size ink particles which fabricated using non-aqueous base modified emulsion process and micron-scale particles by non-solvent particle fabrication process are discussed. Finally, specially designed particles and panel structure fabricated considering the interactions between particle/particle, particle/media or particle/electrode dramatically reduce the driving voltages to ${\pm}$ 10V and improve the response time of less than 100msec and white reflectance of 58% for EPD using dielectric fluid as a medium. In case of EPD adapting micron-sized electrophoretic particles and a medium of air, the saturation voltage could be reduced to ${\pm}$ 40V and having white reflectance of 45% without scarification of electrophoretic mobility of the particles.

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실험동물에 유발시킨 피부사상균증에 대한 연구 (Study of the Experimental Dermatophyte Infection in Animals)

  • 최종수;황계영;김기홍;김성광;정재규;서순봉
    • Journal of Yeungnam Medical Science
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    • 제4권1호
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    • pp.81-87
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    • 1987
  • 피부사상균증의 접종방법을 표준화하기 위하여 N. gypsea "+"와 A. benhamiae "+"를 가토, 기니픽, rat, mouse에 정량적으로 접종하여 다음의 결과를 얻었다. 1. 포자의 농도가 높을수록 감염률이 높았고 입상양상이 심하고 일찍 나타나서 오래 지속되었다. 2. 난전, 소피, 강제발모, 단순빈도의 순으로 감염이 잘되어 피부손상의 정도에 비례함을 알 수 있었다. 3. 피부사상균에 대한 감수성은 기니픽, 가토, rat의 순으로 높았고 mouse는 전혀 감염되지 않았다. 4. N. gypsea "+"에 비해 A. benhamiae "+"는 감염률이 높고 입상양상이 더 심하고 빨리 진행되어 조기에 조절되었다.

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