• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.1
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• pp.121-127
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• 2013

A vision based hand-gesture interface method for substituting a pointing device is proposed in this paper, which is used the ratio and size variation of Gesture Region. Proposed method uses the skin hue&saturation of the hand region from the HSI color model to extract the hand region effectively. This method can remove the non-hand region, and reduces the noise effect by the light source. Also, as the computation quantity is reduced by detecting not the static hand-shape recognition, but the ratio and size variation of hand-moving from the clipped hand region in real time, more response speed is guaranteed. In order to evaluate the performance of the our proposed method, after applying to the computerized self visual acuity testing system as a pointing device. As a result, the proposed method showed the average 86% gesture recognition ratio and 87% coordinate moving recognition ratio.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.6
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• pp.463-471
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• 2000

A pot experiment was carried out to find out the effects of the sludge application on corn and Chinese cabbage growth and changes in soil chemical properties with sludges collected from 4 plants at 0, 12.5, 25, 50, and $100Mg\;ha^{-1}$ levels and chemical fertilizer. With the corn experiment, the pot where sludge and chemical fertilizers were treated together, greater amount of sludge resulted in initial growth inhibition. In general, higher sludge treatment rates resulted in better growth in the end, whereas initial growth was inhibited due to high the electrical conductivity of saturated extracts(ECe) for the Chinese cabbage. However, the highest yield among sludge treatments was lower than the yield with chemical fertilizers. While the treatments resulted in chemical changes in soil showing differences of cation exchange capacity, organic matter contents, and nitrogen contents, hardly any changes were detected before and after crops were grown. Inorganic nutrients such as Na, K, Ca and Mg showed similar changes. The ECe in soil saturation extract decreased after crops were grown. The more sludge was treated, the greater was the decrease. The differences of ECe in the soil saturation extract with varying degrees of treatment were also reduced after crops were grown. Available phosphorus content increased during growth. Due to the low nitrogen content in sludge, when nitrogen becomes the determining factor for the amount of sludge treatment, phosphorus buildup resulted from continued application of sludge could be raised. Therefore, it is advisable to use phosphorus, not nitrogen content, in determining the amount of sludge treatment and chemical fertilizer as supplementary sources for nitrogen.

• KSBB Journal
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• v.24 no.1
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• pp.65-69
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• 2009

The biological production of a potent anticancer agent, epothilone, by Sorangium cellulosum was carried out using flask and fermentor cultures. Soluble starch was selected as the main carbon source and the concentrations of lactose and yeast extract were optimized at 4 and 0 g/L, respectively, when using the flask cultures. In the fermentor cultures, the cells were cultivated at a high DO level of more than 80% of air saturation in the growth stage and then the DO level was controlled at about 50, 20 or 1-2% when the carbon source was exhausted. The epothilone production increased with decreasing DO level after the exhaustion of the carbon source, and the maximum concentration of epothilone was 5.4 mg/L. It was found that the DO level had significant regulation effects on the epothilone production.

• Korean Chemical Engineering Research
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• v.61 no.2
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• pp.287-295
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• 2023

Iron nanoparticles (Fe-NPs) were synthesized employing Lagenaria siceraria (LS) leaf aqueous extract as a reducing and capping medium to remove methylene blue (MB) dye and have antibacterial properties against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus). The formation of LS-Fe-NPs (Lagenaria-siceraria-iron-nanoparticles) was confirmed by a change in color from pale yellow to dark brown. Characterization techniques, such as particle size analysis (PSA), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), were employed to prove nano spherical particles of size range between 80-100 nm. Phytochemicals and the presence of iron in LS-Fe-NPs nanoparticles were proved by UV-visible spectrophotometry. Further, Fourier transform infrared spectroscopy (FTIR) analysis results confirmed the existence of bioactive molecules in the plants. The magnetic property was analyzed using a vibrating sample magnetometer (VSM), which displayed that the synthesized nanoparticles were superparamagnetic and exhibiting a saturation magnetization of 12.5 emu/g. Synthesized magnetic nanoparticles were used in methylene blue (MB) dye removal through adsorption. About 83% of 100 mg/L MB dye was removed within 120 min at pH 6 with a maximum adsorption capacity of 246.8 mg/g. Antibacterial efficacy of LS-Fe-NPs was screened against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus), respectively, and found that LS-Fe-NPs were effective against Staphylococcus aureus.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.12
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• pp.1122-1129
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• 2015

This paper suggests a method of detecting traffic lights for vehicles by combining the HSV(hue saturation value) color model, morphometric characteristics, and location information appearing on consecutive images in daytime. In order to detect the traffic light, the color corresponding to the signal lights should be explored. It is difficult to detect traffic lights among colors of lights from buildings, taillight of cars, leaves, placards, etc. The proposed algorithm searches for the traffic lights from many candidates using morphometric characteristics and location information in consecutive images. The recognition process is divided into three steps. The first step is to detect candidates after converting RGB channel into HSV color model. The second step is to extract the boundaries between the housing of traffic lights and background by exploiting the assumption that the housing has lower brightness than the surrounding background. The last step is to recognize the signal light after eliminating the false candidates using morphometric characteristics and location information appearing on consecutive images. This paper demonstrates successful detection results of traffic lights from various images captured on the city roads.

• Transactions of the Korean hydrogen and new energy society
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• v.17 no.4
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• pp.371-378
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• 2006

Effect of $(NH_4)_2SO_4$ precipitation and heat-treatment on hydrogenase which was extracted from the cytoplasmic fraction of the phototrophic purple sulfur bacterium Thiocapsa roseopersicina NCIB 8347 was studied. Crude enzyme extract was prepared by centrifugation($28,000{\times}g$, $400,000{\times}g$) after sonication of cells grown under photosynthetic condition for 96 hrs. Various conditions of $(NH_4)_2SO_4$ precipitation and heat-treatment were examined and the effect of protein concentration was analyzed by SDS-electrophoresis between the treatments. Optimum conditions for $(NH_4)_2SO_4$ precipitation and heat-treatment for evolution hydrogenase activity were 40-60% saturation and $60^{\circ}C$ for 20 min, respectively, which exhibited the specific hydrogenase activity of 0.78 U/mg-protein. Specific hydrogenase activity was decreased to 31.6% when the heat-treatment at $60^{\circ}C$ increased from 20 min to 5 hrs.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.7
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• pp.892-897
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• 2018

Due to population aging, an increase in the number of patients with chronic illnesses, and an increase in the number of single-person households, monitoring of health status in everyday life without the need for a hospital has become very important. For this reason, researches on various health care devices have been attempted, among which wearable devices are attracting much attention. In this paper, we propose a new ring-type wearable device for next generation healthcare. On the inner side of the ring, a metal electrodes for GSR measurement and an optical sensor for measurement of pulse wave signals of two wavelengths of red and near-infrared light were mounted. In addition, it was equipped with an acceleration sensor, and information about the degree of motion could be obtained. In this paper, it is shown that a health monitoring device can be implemented in the form of a ring, and the measured signals can be used to calculate heart rate, oxygen saturation, sleep time and sleep efficiency. Through the advanced algorithm, it is expected that we can extract various health information, especially sleep related health information by using the ring device, and it is also expected that it can contribute to the implementation of wearable healthcare effectively.

• Journal of Pharmaceutical Investigation
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• v.32 no.4
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• pp.285-290
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• 2002

In order to assay the efficacy of newly synthesized antiviral compounds, in vitro studies of their active intracellular phosphorylated metabolites were established as compared with Zidovudine (ZDV). Antiviral base analogs require intracellular phosphorylation prior to the inhibition of HIV replication. Therefore, antiviral drugs concentrations in plasma have not reflected any direct relationship with activity or toxicity. A method has been developed to measure the concentration of total phosphorylated metabolites inside peripheral blood mononuclear cells using modified commercial radioimmunoassay (RIA). ZDV 5'-monophosphate was synthesized and used as a procedural control for RIA modification. PBMCs were isolated from whole blood and incubated with ZDV for 20 h to allow metabolic phosphorylation. Viable cells were extracted overnight with 60% methanol. After evaporation, the extract was reconstituted in Tris buffer. Samples were split into two fractions, one of which was treated with alkaline phosphatase (AP) to liberate phosphate groups. Concentrations of phosphorylated metabolites were determined by subtracting thε concentration of non-AP-treated fraction from that of the treated fraction. Recovery of phosphorylated ZDV from cell extracts was approximately 90%, and reproducibility was acceptable (coefficients of variation <15% for concentrations${\geq}$0.25 ng/mL). Intracellular concentrations $(0.135{\sim}5.019\;nmole/10^6\;cells)$ followed a nonlinear dose-response relationship over the range $0.015{\sim}2.996mM$ extracellular ZDV, with concentration-dependant saturation.

• Journal of Broadcast Engineering
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• v.18 no.4
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• pp.550-558
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• 2013

The infrared and visible images are represented by different information due to the different wavelength of the light. The infrared image has thermal information and the visible image has texture information. Desirable results are obtained by fusing infrared and visible information. To enhance a visible image, we extract a weight map from a visible image using saturation, brightness. After that, the weight map is adjusted using thermal information in the infrared image. Finally, an enhanced image is resulted from combining an infrared image and a visible image. Our experiment results show that our proposed algorithm is working well to enhance the smoke in the original image.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.3
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• pp.315-322
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• 2005

In this study we report on the estrogen activity of silkworm pupa and herb extracts in vitro. The estrogenic activity of these resources was investigated by competition binding assays with estrogen receptor $\alpha(ER{\alpha})\;or\;ER{\beta}$, and viability of MCF-7 cells, a human breast cancer cell line. Saturation ligand-binding analysis of $ER{\alpha}\;and\;ER{\beta}$ revealed that all plant extracts competed with estrogen ligand for binding to both ER subtypes with a similar preference and degree and competed stronger with ligand for binding to $ER{\beta}\;than\;to\;ER{\alpha}$. The highest $ER{\alpha}-binding$ sample was silkworm pupa aqueous extract The highest $ER{\beta}-binding$ sample was silkworm pupa oil. These samples were further tested for bioactivity based on their ability to regulate cell growth rate in ER(+) breast cancer cell line, MCF-7 cells. Our studies showed that silkworm pupa, soritae, sesame, yam, pueraria, malt, ginseng, Polygonum multiflorum, and Curcuma longa significantly stimulated the growth of MCF-7 cells (P<0.05). In summary, these results suggested that silkworm pupa and herbs might be useful as potential phytoestrogens.