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Effect of Dissolved Oxygen on the Production of Epothilone in Bioreactor Cultures Sorangium cellulosum  

Park, Su-Jeong (Department of Chemical Engineering, Sungkyunkwan University)
Han, Se-Jong (Department of Chemical Engineering, Sungkyunkwan University)
Kim, Byung-Woo (Department of Chemical Engineering, Sungkyunkwan University)
Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
Publication Information
KSBB Journal / v.24, no.1, 2009 , pp. 65-69 More about this Journal
Abstract
The biological production of a potent anticancer agent, epothilone, by Sorangium cellulosum was carried out using flask and fermentor cultures. Soluble starch was selected as the main carbon source and the concentrations of lactose and yeast extract were optimized at 4 and 0 g/L, respectively, when using the flask cultures. In the fermentor cultures, the cells were cultivated at a high DO level of more than 80% of air saturation in the growth stage and then the DO level was controlled at about 50, 20 or 1-2% when the carbon source was exhausted. The epothilone production increased with decreasing DO level after the exhaustion of the carbon source, and the maximum concentration of epothilone was 5.4 mg/L. It was found that the DO level had significant regulation effects on the epothilone production.
Keywords
Dissolved Oxygen (DO); Epothilone; Secondary metabolite; Sorangium cellulosum;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Julien, B., S. Shah, R. Ziermann, R. Goldman, and L. Katz, and C. Khosla(2000), Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum, Gene 249, 153-160   DOI   PUBMED   ScienceOn
2 Bollag, D. M., P. A. McQueney, J. Zhu, O. Hensens, L. Koupal, J. Líesch, M. Goetz, E. Lazarides, and C. M. Woods (1995), Epothilones, a new class of microtubule-stabilizing agents with a taxol-like mechanism of action, Cancer Res, 55, 2325-2333   PUBMED
3 Rowinsky, E. K., E. A. Eisenhauer, V. Chaudhry, S. G. Arbuck, and R. C. Donehower (1993), Clinical toxicities encountered with paclitaxel (Taxol), Semin. Oncol. 20, 1-15   PUBMED
4 Park, S. W., S. J. Han, D.-S. Kim, and S. J. Sim (2007), Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture, Biochem. Eng. J. 37, 328-331   DOI   ScienceOn
5 Pfeifer, B. A. and C. Khosla (2001), Biosynthesis of polyketides in heterologous hosts, Microbiol. Mol. Biol. Rev. 65, 106-118   DOI   ScienceOn
6 Tang, L., S. Shah, L. Chung, J. Camey, L. Katz, C. Khosla, and B. Julien (2000), Cloning and heterologous expression of the epothilone gene cluster, Science 287, 640-642   DOI   PUBMED
7 Reichenbach, H. and M. Dworkin (1992), The myxobacteria, In The Procaryotes, A. Balow, H. G. Truper, M. Dworkin, W. Harder, and K. H. Schleifer, Eds., p3416, Springer-Verlag, New York
8 Han, S. J., S. W. Park, B. W. Kim, and S. J. Sim (2008), Selective production of epothilone B by heterologous expression of propionyl-CoA synthetase in Sorangium cellulosum, J. Microbiol. Biotechnol. 18, 135-137   PUBMED   ScienceOn
9 Gerth, K., N, Bedorf, G. Htlc, H. Irschik, and H. Reichεnbach (1996), Epothilones A and B: Antifungal and cytotoxic compounds from Sorangium cellulosum (myxobacteria) Production, physico-chemical, and biological propertics, J. Antibiot. 49, 560-563   DOI   PUBMED
10 Park, S. W., S. J. Park, S. J. Han, J. Lee, D.-S. Kim, J.-H. Kim, B. W. Kim, J. Lee, and S. J. Sim (2007), Repeated batch production of epothilone B by immobilized Sorangium cellulosum, J. Microbiol. Biotechnol. 17, 1208-1212   과학기술학회마을   PUBMED   ScienceOn
11 Su, D.-S., A. Balog, D. Meng, P. Bertinato, S. J. Danishfsky, Y.-H. Zheng, T.-C. Chou. L. He, and S. B. Horwitz (1997), Structure-activity relationships of the epothilones and the first in vívo comparison with paclitaxel, Angew. Chem. Int. ED. Engl. 36, 2093-2096   DOI   ScienceOn
12 Molnal, I., T. Schupp, M. Ono, R. Zirkle, M. Milnamow, B. Nowak-Thomapson, N. Engel, C. Toupet, A. Stratmann, D. D. Cyr, J. Gorlach, J. M. Mayo, A. Hu, S. Goff, J. Schmid, and J. M. Ligon (2000), THe Biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90, Chem. Biol. 7. 97-109   DOI   ScienceOn
13 Park, S. w., S. H. Choi, Y. J. Yoon, D. H. Lee, D. J. Kim, J.-H. Kim, Y. K. Lee, G. J. Choi, I.-T. Yeom, and S. J. Sim (2006), Enhanced production of epothilones by carbon sources in Sorangium cellulosum, J. Microbiol. Biotechnol. 16, 519-523
14 Rosenberg, E., K. H. Keller, and M. Dworkin (1977), Cell density-dependent growth of Myxococcus xanthμs on casein, J. Bacteriol. 129, 770-777   PUBMED