Epigenetic modification including genome-wide DNA demethylation is essential for normal embryonic development. Insufficient demethylation of somatic cell genome may cause various anomalies and prenatal loss in the development of nuclear transfer embryos. Hence, the source of nuclear donor often affects later development of nuclear transfer (NT) embryos. In this study, appropriateness of porcine embryonic germ (EG) cells as karyoplasts for NT with respect to epigenetic modification was investigated. These cells follow methylation status of primordial germ cells from which they originated, so that they may contain less methylated genome than somatic cells. This may be advantageous to the development of NT embryos commonly known to be highly methylated. The rates of blastocyst development were similar among embryos from EG cell nuclear transfer (EGCNT), somatic cell nuclear transfer (SCNT), and intracytoplasmic sperm injection (ICSI) (16/62, 25.8% vs. 56/274, 20.4% vs. 16/74, 21.6%). Genomic DNA samples from EG cells (n=3), fetal fibroblasts (n=4) and blastocysts from EGCNT (n=8), SCNT (n=14) and ICSI (n=6) were isolated and treated with sodium bisulfite. The satellite region (GenBank Z75640) that involves nine selected CpG sites was amplified by PCR, and the rates of DNA methylation in each site were measured by pyrosequencing technique. The average methylation degrees of CpG sites in EG cells, fetal fibroblasts and blastocysts from EGCNT, SCNT and ICSI were 17.9, 37.7, 4.1, 9.8 and 8.9%, respectively. The genome of porcine EG cells were less methylated than that of somatic cells (p<0.05), and DNA demethylation occurred in embryos from both EGCNT (p<0.05) and SCNT (p<0.01). Interestingly, the degree of DNA methylation in EGCNT embryos was approximately one half of SCNT (p<0.01) and ICSI (p<0.05) embryos, while SCNT and ICSI embryos contained demethylated genome with similar degrees. The present study demonstrates that porcine EG cell nuclear transfer resulted in hypomethylation of DNA in cloned embryos yet leading normal preimplantation development. Further studies are needed to investigate whether such modification affects long-term survival of cloned embryos.
Journal of the Korean Association of Geographic Information Studies
/
v.9
no.4
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pp.12-21
/
2006
Recently, Very large and high resolution geological data from aerial or satellite imagery are available. Many researches and applications require to do realtime visualization of interest geological area or entire planet. Important operation of wide-spreaded terrain realtime visualization technique is the appropriate model resolution selection from pre-processed multi-resolution model hierarchy depend upon participant's view. For embodying such realtime rendering system with large geometric data, Preprocessing multi-resolution hierarchy from large scale geological information of interest area is required. In this research, recent Cubic multiresolution 4-8 tile hierarchy is selected for global planetary applications. Based upon the tile hierarchy, It constructs the selective terminal level tile mesh for original geological information area and starts to sample individual generated tiles for terminal level tiles. It completes the hierarchy by constructing intermediate tiles with low pass filtering in bottom-up direction. This research embodies series of efficient cubic 4-8 tile hierarchy construction mechanism with out-of-core storage. The planetary scale Mars' geographical altitude data and image data were selected for the experiment.
Journal of the Korean Institute of Landscape Architecture
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v.46
no.1
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pp.115-126
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2018
A new urban paradigm that moves from a vehicle-centric to pedestrian-centric culture should be considered to improve the quality of the pedestrian environments for women, children, senior citizens, and disabled persons as well as to promote community unification by providing general movement rights to everyone. This study was implemented to provide decent alternatives to improve street spaces. The street spaces around the Commercial and Residential Area No.1 located in the Administrative-Centered City, Sejong Special Autonomic City, were selected to analyze and define the status of the walkways and the street spaces. Satellite imagery and numerical maps were used to collect geographic data. Practical and actual surveys for the selected sites were performed to analyze the street status and the pedestrian status. Based on the all collected data, analysis results, and literature reviews, the questionnaire was made, and 315 inquiries qualified for analysis. The physical status of all four study sites was the highest level, Grade A, and green open spaces were relatively sufficient. As a result, the factors obtained from the factor analysis have an impact on the satisfaction of the pedestrian streets in the commercial area. The factors are as followed Design > Convenience > Roadside trees and rest areas > Safety > Safety protective facilities > Transportation and information facilities > Continuity > Basic state of road surfaces > Comfortability, and in the residential area: Transportation and information facilities > Basic state of road surfaces > Comfort > Convenience > Continuity > Design > Illumination and crime prevention facilities > Safety > Roadside trees and rest areas.
Land suitability assessment(LSA) is a useful method to assess land characteristics according to its physical, locational, and environmental characteristics, and then to classify it into several categories based on its usability and the necessity for conservation. Therefore, the credibility of this assessment result depends on the rationality of assessment method such as computation of suitability values and the criteria of ranking the land grade. This study focused on identifying problems in current LSA method by critically reviewing it, and aimed to suggest a suitable method for the assessment of land suitability. As a alternative method in this study, linear transformation method was used for scoring factors instead of critical values and the weight for each assessment factor. and all values of those factors were added to produce comprehensive suitability values. Those values were ordered and categorizied with suitability level based on the ratio of square measure. After comparing existing LSA method to the suggested alternative method, it is concluded that the alternative method was more suitable to assess the characteristics of lands than existing LSA method. This method was also suitable to represent the characteristics of various assessment factors. Field survey using satellite image clearly revealed the suitability of the alternative method compared to an existing method of land assessment. Furthermore, the alternative method of grading the land suitability improves the problem of using standardiZiation method and gives more flexibility, which makes this method very rational.
Journal of the Korean Institute of Landscape Architecture
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v.46
no.3
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pp.27-35
/
2018
In the mandatory application of the CPTED, only negative parts of urban parks and vegetation were reflected. Therefore, this study tries to present the positive effects of urban parks and vegetation. The purpose of this study is to demonstrate the effects of urban parks and vegetation on crime occurrence and to suggest the planning implications of this to CPTED based on theory related to crime, environmental psychology, and crime occurrence analysis. This study used the crime occurrence data of Seoul, NDVI, population, distance from urban park, floating population, and the like. This study collected data from the Statistics Korea, the local government, and Landsat 8 satellite images provided by the USGS and created data of environmental variables and social variables by district using ArcGIS and statistical program. Literature analysis, correlation analysis, regression analysis, and geographically weighted regression were used to determine the relationship between crime occurrence and environmental variables, and to discuss its implication. It was found that crime occurrence has a relationship with the total population (${\beta}=.663$), the number of amusement facilities (${\beta}=.447$) and the area of a police station jurisdiction (${\beta}=.395$). This confirms that a crime rate is low when the floating population is large (${\beta}=-.241$) and vegetation vitality is high (NDVI, ${\beta}=-.281$). Vegetation vitality (NDVI) is effective in lowering violence through psychological stabilization, strengthening territoriality and improving regional image. The implications for the allocation of urban park and vegetation, program and management plan of urban park and vegetation to reduce crime occurrence have therefore been presented.
This study examines the impacts of land cover changes on the East Asia summer monsoon with the National Center for Atmospheric Research Regional Climate Model (NCAR RegCM2), coupled with Biosphere Atmosphere Transfer Scheme (BATS). To assess the goals, two types of land cover maps were used in the simulation of summer climate. One type was NCAR land cover map (CTL) and the other was current land cover map derived from satellite data (land cover: LCV). Warm and cold surface temperature biases of $1-3^{\circ}C$ occurred over central China and Mongolia in CTL. The model produced excessive precipitation over northern land area but less over southern ocean of the model domain. Changes of biophysical parameters, such as albedo, minimum stomatal resistance and roughness length, due to the land cover changes resulted in the alteration of land-atmosphere interactions. Latent heat flux and wind speed in LCV increased noticeably over central China where deciduous broad leaf trees have been replaced by mixed farm and irrigated crop. As a result, the systematic warm biases over central China were greatly reduced in LCV. Strong cooling of central China decreased pressure gradient between East Asian continent and Pacific Ocean. The decreased pressure gradient suppressed the northward transport of moisture from south China and South China Sea. These changes reduced not only the excessive precipitation over north China and Mongolia but also less precipitation over south China. However, the land cover changes increased the precipitation over the Korean Peninsula and the Japan Islands, especially in July and August.
In this study, the oceanic Total Precipitable Water (TPW) retrieval algorithm at 16 km altitude of High Altitude Long Endurance Unmanned Aerial Vehicle (HALE UAV) is described. Empirical equation based on Wentz method (1995) that uses the 18.7 and 22.235 GHz channels is developed using the simulated brightness temperature and SeeBor training dataset. To do radiative simulation, Satellite Data Simulator Unit (SDSU) Radiative Transfer Model (RTM) is used. The data of 60% (523) and 40% (349) in the SeeBor training dataset are used to develop and validate the TPW retrieval algorithm, respectively. The range of coefficients for the TPW retrieval at the altitude of 3~18 km with 3 km interval were 153.69~199.87 (${\alpha}$), 54.330~58.468 (${\beta}$), and 84.519~93.484 (${\gamma}$). The bias and RMSE at each altitude were found to be about $-0.81kg\;m^{-2}$ and $2.17kg\;m^{-2}$, respectively. Correlation coefficients were more than 0.9. Radiosonde observation has been generally operated over land. To validate the accuracy of the oceanic TPW retrieval algorithm, observation data from the Korea Meteorological Administration (KMA) Gisang 1 research vessel about six clear sky cases representing spring, autumn, and summer season is used. Difference between retrieved and observed TPW at 16 km altitude were in the range of $0.53{\sim}1.87kg\;m^{-2}$, which is reasonable for most applications. Difference in TPW between retrieval and observation at each altitude (3~15 km) is also presented. Differences of TPW at altitudes more than 6 km were $0.3{\sim}1.9kg\;m^{-2}$. Retrieved TPW at 3 km altitude was smaller than upper level with a difference of $-0.25{\sim}0.75kg\;m^{-2}$ compared to the observed TPW.
Recently high spatial resolution space-borne Synthetic Aperture Radar (SAR) systems have launched and have been operated successfully. Interferometric SAR (InSAR) processing with the space-based high resolution observations acquired by these systems can provide more detail information for various geodetic applications. Coherence is regarded as a critical parameter in the evaluating the quality of an InSAR pair. In this study, we evaluate the coherence characteristics of high-resolution data acquired by TerraSAR-X (X-band) and ALOS PALSAR (L-band) and intermediate-resolution data acquired by Envisat ASAR (C-band) over western Texas, U.S.A. Our coherence analysis reveals that the high-resolution X-band TSX (3.1 cm) data has a high coherence level (0.3-0.6), similar to that of the L-band ALOS PALSAR data (23.5 cm) in short temporal baselines. Further more, the TSX coherence values are significantly higher than those of the C-band (5.6 cm) Envisat ASAR data. The higher coherence of the TSX dataset is a surprising result, because common scattering theories suggest that the longer wavelength SAR data maintain better coherence. In vegetated areas the shorter wavelength radar pulse interacts mostly with upper sections of the vegetation and, hence, does not provide good correlation over time in InSAR pairs. Thus, we suggest that the higher coherence values of the TSX data reflect the data's high-resolution, in which stable and coherent scatters are better maintained. Although, however, the TSX data show a very good coherence with short temporal baseline (11-33 days), the coherences are significantly degraded as the temporal baselines are increased. This result confirms previous studies showing that the coherence has a strong dependency on the temporal baseline.
Purpose of this study was to estimate of soil erosion, which is related with crop productivity and water quality in watershed, in Seonakdong river watershed using USLE. The data set for USLE estimation were derived from detailed digital map(K factor), satellite imagery(C and P factors) and DEM(LS factor). The R factor was calculated by AWS data from Kimhae agricultural technology center. The soil loss from arable land was equivalent of 31.5% of total soil loss in Seonakdong river watershed. The soil loss amount of paddy field and upland were 2.8% and 97.2% of arable land, respectively, even in the area where paddy field was occupied much largely as 76.3%. The reason of large amount of soil loss from upland was that 30.4% of upland was distributed at "severe" and "very severe" soil erosion grade in watershed. The distribution of soil erosion grade during cropping season(May-Sept.) was similar to the annual soil loss. Soil erosion of non-cropping season(Oct.-Apr.) was small due to a low R factor. But, soil erosion grade of near mountain footslope areas showed severe and very severe even in non-cropping season.
Journal of the Korean Association of Geographic Information Studies
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v.21
no.3
/
pp.162-175
/
2018
The purpose of this study was to analyze the surface temperature of surface fabrics using UAV TIR images, to mitigate problems in the thermal environment of urban areas. Surface temperature values derived from UAV images were compared with those measured in-situ during the similar period as when the images were taken. The difference in the in-situ measured and UAV image derived surface temperatures is the highest for gray colored concrete roof fabrics, at $17^{\circ}C$, and urethane fabrics show the lowest difference, at $0.3^{\circ}C$. The experiment power of the scatter plot of in-situ measured and UAV image derived surface temperatures was 63.75%, indicating that the correlation between the two is high. The surface fabrics with high temperature are metal roofs($48.9^{\circ}C$), urethane($43.4^{\circ}C$), and gray colored concrete roofs($42.9^{\circ}C$), and those with low temperature are barren land($30.2^{\circ}C$), area with trees and lawns($30.2^{\circ}C$), and white colored concrete roofs($34.9^{\circ}C$). These results show that accurate analysis of the thermal characteristics of surface fabrics is possible using UAV images. In future, it will be necessary to increase the usability of UAV images via comparison with in-situ data and linkage to satellite imagery.
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