• 한방안이비인후피부과학회지
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• 제23권1호
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• pp.158-168
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• 2010

Objective : Jeungson-Ojeok-Hwan Bijukbang(JOH) has been used to treat patients with tumor etc. In the theory of Korean medicine, JOH can treat Juk-Chui. JOH is known to have treat Juk-Chui. Juk-Chui is analogous to tumor. Methods : For these reasons, the present study was designed to investigate the effects of JOH on solid tumor in mice in terms of immune-potentiating and direct cytotoxic action of JOH in vitro and vivo study. The present author investigated thymocyte and splenocyte proliferation to confirm immune-potentiating activity of JOH and also investigated tumor/body weight ratio and survival rates in tumor bearing mice. Results : In this study, administration of JOH decreased tumor/body weight ratio significantly, and prolonged survival duration compared to non-treated control. In addition, treatment with JOH suppressed proliferation rate of Sarcoma 180 (S-180) cells significantly, and elevated proliferation rates of thymocytes isolated from normal mice. These results were co-related with in vivo study. Conclusion : these results suggest that JOH is useful to treat patient with solid tumor, because JOH has direct toxic action for tumor cell and immune-potentiating action for T cells. Further study will need to elucidate exact mechanisms related in anti-cancer action of JOH.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.132-144
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• 2002

Shinsuwuisaeng-Tang was a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effect of Shinsuwuisaeng-Tang on the anti-cancer and nitric oxide(NO) production of peritoneal macrophages. We used Shinsuwuisaeng-Tang extract(SWT) with freeze-dried, 8wks-old male mice. and cancer cell lines(L1210, sarcoma-180) for this Study. The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follow; SWT was showed cytotoxicity on the L1210 and sacoma-180(S-180) cell lines, SWT inhibited significantly proliferation of L1210 cells in L1210 cells transplanted mice, SWT accelerated NO production of peritoneal macrophages in L1210 cells transplanted mice. And SWT inhibited significantly tumor weight, increased significantly body weight and mean survival days in S-180 cells transplanted mice. This results suggest that SWT has anti-cancer by producing NO of peritoneal macrophages.

• 한방안이비인후피부과학회지
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• 제19권1호
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• pp.93-102
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• 2006

Objective : Gamisibgi-San was a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effects of Gamisibgi-San on the anti-cancer and proliferation of immunocytes. Materials and Method : We used Gamisibgi-San extract(GMSGS) with freeze-dried, 8wks-old male mice and cancer cell lines(L1210, S-180) for this Study. The cytotoxicity and proliferation of cells wat tested using a colorimetric tetrazoliun assay(MIT assay). Results and Conclusion : The results of this Study were obtained as follow ; 1. GMSGS was significantly showed cytotoxicity on the L1210 cell lines and S-180 cell lines. 2. GMSGS was significantly increased in the proliferation of thymocytes and splenocytes in vitro. 3. GMSGS was significantly decreased in the proliferation of L1210 cells in L1210 cells transplanted mice. 4. GMSGS was significantly decreased in the Weight of Sarcoma in S-180 cells transplanted mice. 5. GMSGS was significantly increased in the Period of Survive in S-180 cells transplanted mice. The present author thought that GMSGS had action of anti-cancer by becoming immunocytes activity(proliferation of thymocytes and splenocytes).

• 한국균학회지
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• 제39권1호
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• pp.57-67
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• 2011

신령버섯은 담자균문, 주름버섯목, 주름버섯과에 속하는 식용버섯으로 혈당, 혈압강하효과와 콜레스테롤 저하, 항종양, 암예방 효과가 있는 것으로 알려져 있다. 신령버섯의 자실체로부터 메탄올, 중성염용액 및 열수 등을 이용하여 조다당류를 추출하고 성분을 분석한 결과 ${\beta}$-glucan은 21.54~32.31%, 단백질은 0.16~9.34%로 구성되어 있는 것이 밝혀졌고 신령버섯 추출 조다당류를 Sarcoma 180, HT-29, NIH3T3 및 RAW 264.7 등의 암세포 및 정상세포에 대한 독성을 조사한 결과 10~2000 ${\mu}g/ml$의 조다당류 농도에서 각각의 세포는 세포독성을 나타내지 않았다. Sarcoma 180으로 접종된 ICR 생쥐에 신령버섯의 자실체에서 추출한 각각의 조다당류를 투여한 실험군은 대조군에 비해 수명이 각각 18.8~50.6% 연장되었다. 비장세포의 증식능과 B 임파구의 활성화에 미치는 alkaline phosphatase의 활성을 조사한 결과 비장 세포 증식능은 대조군에 대해 1.1~1.2배의 증식능을 보였고 B 임파구의 활성은 대조군에 대해 1.2~1.6배 증가하였다. 또한 버섯추출 조다당류를 투여한 실험군이 대조군에 비하여 대식세포에서 1.3~4.3배의 많은 nitric oxide를 발생시켰다. 비장세포에 여러 농도의 조다당류를 처리한 후 TNF-${\alpha}$, IL-$1{\beta}$, IL-2 및 IL-6 등의 사이토카인 분비량을 측정한 결과 생성된 사이토카인의 양은 대조군에 비해 2.2배 높게 나타났다. 중성염용액추출 조다당류를 50 mg/kg body weight의 농도로 투여한 실험군 생쥐의 총 복강 세포 수는 대조군에 비하여 4배 증가하였고, 열수추출 조다당류를 50 mg/kg body weight의 농도로 투여한 실험군 생쥐의 백혈구 수는 대조군에 비하여 각각 2.7배 증가하였다. 혈액생화학적 검사를 시행한 결과, 대조군과 유사한 경향을 나타냈다. 따라서 신령버섯의 자실체에서 추출한 조다당류는 생쥐의 면역을 증강시키는 것은 물론 Sarcoma 180에 대한 항암효과를 나타내는 것으로 밝혀졌다.

• 한국임상수의학회지
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• 제24권3호
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• pp.372-378
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• 2007

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain hepatitis chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillarix extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells on in vivo experimental tests. On in vivo experimental tests using 280 ICR mice the gain of body weight in the control-group mice bearing Sarcoma 180 ascites tumor was 1.5 times more than that of the normal-group mice after 33 days. However, the gain of body weight in all experimental groups administered with Artemisia capillaris extracts was significantly lower than that of the control-group mice (P<0.05). The mean survival times of mice administered with Artemisia capillaris extracts of 25 and 100 mg/kg for 28 days were shown to be 25.39% and 15.39% longer than that of the control-group mice injected with saline (P<0.05). Artemisia capillaris extracts showed the highest tumor inhibition effects, which were 42.4% and 27.2% when intraperitoneally injected with doses of 25 and 100 mg/kg once a day for 28 days in inoculated ICR mice with Sarcoma 180 solid tumor cells (P<0.05). The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• IMMUNE NETWORK
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• 제2권2호
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• pp.115-124
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• 2002

Background: The chemical characteristics of the exo-secretion from Phellinus linteus (referred to as exo-secretion) including the compositions of amino acids and monosaccharides were investigated. In addition, cytotoxicity of the exo-secretion on 5 tumor cell lines derived from human cancers and its antitumor activity against ascitic sarcoma-180 cells were examined. Methods: The antitumor activity of exo-secretion from Phellinus linteus was determined by measuring parameters including tumor weight, life span of mice, chemotatic activity of leukocytes, counts of immune cells, and activity of cytokines. Results: The exo-secretion from Phellinus linteus showed no direct cytotoxicity to the five tumor cell lines tested, but it had a strong antitumor activity against sarcoma-180 cells in ICR mice as measured by tumor weight and life span of mice. The exo-secretion stimulated the chemotaxis of leukocytes and production of immune cells and cytokines. Conclusion: These results suggest that the exo-secretion from Phellinus linteus do not act as a direct cytotoxic substance to cancer cells but as an immunomodulator.

• 약학회지
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• 제42권3호
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• pp.274-274
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• 1998

Aclarubicin(ACL)-entrapped freeze dried liposomes were prepared using Microfludizer to attain a sustained release at targeted organs in a prolonged time so that it can reduce th e side effect and maximize the therapeutic effect. The freeze-dried liposomes were evaluated for pharmacokinetics, antitumor activity against Sarcoma 180, cytotoxicity against L1210 and A549 tumor cells, spleen toxicity and myelosuppressive action. The AUC0->8hr values were 122+/-42, 382+/-140, 419+/-171, 835+/-206 and 443+/-309mcg min/ml for free ACL. ACL-liposome formulation I, II, III and IV, respectively. Cytotoidcity of ACL-entrapped liposomes against L1210 and A549 tumor cells was 2-4 times higher than that of free aclarubicin. ACL-liposome formulation I(PC/CHOL/TA) showed the most potent antitumor activity against Sarcoma 180 in mice. The loss of body weight was much smaller with ACL-entrapped liposomes than free ACL after I.p. injection at a dose of 2 mg/kg/day. Compared to free ACL, ACL-entrapped liposomes expressed a lower and delayed spleen toxicity up to 5th day after I.v. administration. Myelosupperssion seemed to be lower with ACL-entrapped liposome of PC/PC-hydrate/CHOL/TA (formulation III) than free aclarubicin.

• 약학회지
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• 제37권1호
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• pp.89-94
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• 1993

In order to find out a proper connecting bridge between 5-fluorouracil(5-FU) and a macromolecule such as a polypeptide, potentially hydrolytic N$^{1}$-derivartives of 5-FU have been systhesized and evaluated for their biological activity. When tested with in vitro leukemic L$_{1210}$ cells all the obtained derivartives exhibited slightly higher antitumor activity than the parent 5FU. Among them the N$^{1}$ -carbamoyl analogue 2 and N$^{1}$-acetamido analogue 6b showed 50% inhibition of the L$_{1210}$ cell growth at the concentrations of 5.01$\times$10$^{-8}$M and 1.03$\times$10$^{-7}$M, respectively. When tested against sarcoma 180 tumor cells inoculated into mice, the compounds 2 and 6b exhibited, respectively, 62% and 54% inhibition of the solid tumor growth at the 5-time doses of 100 mg/kg/day. Both compounds, N$^{1}$-carbamoyl analogue 2 and N$^{1}$-acetamido analogue 6b, realeased the parent 5-FU when incubated in the L$_{1210}$ cell cultural media for 5 hrs.

• 대한한방종양학회지
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• 제2권1호
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• pp.75-90
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• 1996

This experiment was designed to study the antitumor effects and Activity of Natural Killer Cell of semen Tiglii plus Rhizoma Rhei. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, Caki-1, LL2, Sarcoma 180, NIH/3T3) after exposure to prebrewed Semen Tiglii plus Rhizoma Rhei water extract 0.1, 0.2, 0.4, 0.8, 1.6mg/ml using in MTT assay, LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. From the result of MTT assay, the cytotoxicity of ST(生巴豆霜), ST+RR(生巴豆霜加大黃) were concentration-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR(生巴豆霜加大黃) was similar to that of ST(生巴豆霜). 2. From the result of LDH, the cytotoxicity of ST, ST +RR were concentrati -on-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR was similar to that of ST. 3. The antitumor effect on A549 tumor cell from the result of colony forming efficiency showed the inhibitory effect on the growth in both group of the ST and ST+RR, the inhibitory effect on growth was low slightly in the ST+RR. 4. From the result of SRB assay, the antitumor effect on caki-1 tumor cell of ST, ST+RR showed the inhibitory effect on the growth in both group of the ST and ST+RR, the antitumor effect of ST+RR was similar to that of ST. 5. Median survival time and increased life span were increased slightly in both group of the ST and ST+RR. 6. The inhibitory effect on the growth of Sarcoma 180 and Lewis lung carcinoma tumor cell were increased slightly in both group of the ST and ST+RR. 7. The activity of NK cell was increased in the ST+RR.

• 한국식품과학회지
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• 제30권3호
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• pp.702-708
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• 1998

연구는 버섯을 이용한 기능성식품 개발 및 버섯가공 산업 육성에 기초자료를 제공하고자 표고버섯 및 느타리버섯 자실체, 균사체 및 폐상의 조단백다당체 분말을 0, 5, 25 mg/kg 농도로 생리식염수에 용해시켜 마우스(DBA/2와 ICR)에 주사하여 i) 백혈병$(L_{1210})$, 간암$(H_{22}),\;mouser{\;}sarcoma180\;(S_{180})$에 대한 항암효과, ii) 면역기전에 관련된 장기중량변화, 용혈반 형성 세포수 변화 등 조단백다당이 면역에 미치는 영향, 그리고 in vitro 세포독성실험을 수행하였다. 본 실험에서 사용한 모든 조단백다당류는 백혈병에 대해 항암효과를 보였는데 그 중 표고버섯자실체 25 mg/kg 처리구가 가장 높은 저지율(86%)을 보였다. 간암$(H_{22})$에 대한 항암효과는 표고버섯균사체 5 mg/kg 처리구를 제외하고 모든 처리구에서 저지효과를 보였는데, 그 중 느타리버섯 폐상 25 mg/kg 처리구가 가장 높은 저지율(87.6%)을 보였으며, 다음은 표고버섯자실체 25 mg/kg 처리구(71%)였다. Sarcoma180에 대한 항암효과는 표고버섯균사체와 자실체 25 mg/kg 처리구에서 각각 30.9,와 33.9%의 저지율을 보였다. In vitro 세포독성검사에서 각 시료의 최종농도 $50,\;100,\;200,\;400\;{\mu}g/{\mu}L$에서 $L_{1210}$에 대한 유의적 세포사망률은 보이지 않았다. 면역효과 실험에서 간장과 비장중량은 농도증가에 따라 증가하는 추세이나 현저한 차이는 없었다. 표고버섯균사체와 자실체 처리시 항체생성능력을 지닌 용혈반 형성 세포수는 대조군에 비해 현저히 높게 나타났다.