• Structural Engineering and Mechanics
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• 제73권5호
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• pp.555-563
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• 2020

The objective of present paper is assessment of dynamic buckling behavior of an embedded sandwich microplates in thermal environment in which the layers are reinforced through functionally graded carbon nanotubes (FG-CNTs). Therefore, mixture rule is taken into consideration for obtaining effective material characteristics. In order to model this structure much more realistic, Kelvin-Voigt model is presumed and the sandwich structure is rested on visco-Pasternak medium. Exponential shear deformation theory (ESDT) in addition to Eringen's nonlocal theory are utilized to obtain motion equations. Further, differential cubature method (DCM) as well as Bolotin's procedure are used to solve governing equations and achieve dynamic instability region (DIR) related to sandwich structure. Different parameters focusing on volume percent of CNTs, dispersion kinds of CNTs, thermal environment, small scale effect and structural damping and their influences upon the dynamic behavior of sandwich structure are investigated. So as to indicate the accuracy of applied theories as well as methods, the results are collated with another paper. According to results, presence of CNTs and their dispersion kind can alter system's dynamic response as well.

• Computers and Concrete
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• 제24권3호
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• pp.185-192
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• 2019

In this paper, the buckling of micro sandwich hollow circular plate is investigated with the consideration of the porous core and piezoelectric layer reinforced by functionally graded (FG)carbon nano-tube. For modeling the displacement field of sandwich hollow circular plate, the high-order shear deformation theory (HSDT) of plate and modified couple stress theory (MCST) are used. The governing differential equations of the system can be derived using the principle of minimum potential energy and Maxwell's equation that for solving these equations, the Ritz method is employed. The results of this research indicate the influence of various parameters such as porous coefficients, small length scale parameter, distribution of carbon nano-tube in piezoelectric layers and temperature on critical buckling load. The purpose of this research is to show the effect of physical parameters on the critical buckling load of micro sandwich plate and then optimize these parameters to design structures with the best efficiency. The results of this research can be used for optimization of micro-structures and manufacturing different structure in aircraft and aerospace.

• Structural Engineering and Mechanics
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• 제70권5호
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• pp.601-621
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• 2019

This study presents a comprehensive nonlinear dynamic approach to investigate the linear and nonlinear vibration of sandwich plates fabricated from functionally graded materials (FGMs) resting on an elastic foundation. Higher-order shear deformation theory and Hamilton's principle are employed to obtain governing equations. The Runge-Kutta method is employed together with the commercially available mathematical software MAPLE 14 to solve the set of nonlinear dynamic governing equations. Method validity is evaluated by comparing the results of this study and those of previous research. Good agreement is achieved. The effects of temperature change on frequencies are investigated considering various temperatures and various volume fraction index values, N. As the temperature increased, the plate frequency decreased, whereas with increasing N, the plate frequency increased. The effects of the side-to-thickness ratio, c/h, on natural frequencies were investigated. With increasing c/h, the frequencies increased nonlinearly. The effects of foundation stiffness on nonlinear vibration of the sandwich plate were also studied. Backbone curves presenting the variation of maximum displacement with respect to plate frequency are presented to provide insight into the nonlinear vibration and dynamic behavior of FGM sandwich plates.

• Structural Engineering and Mechanics
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• 제83권4호
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• pp.465-473
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• 2022

In this research, a numerical study has been provided for examining the nonlinear stability behaviors of sandwich beams having a cellular core and two face sheets made of nanocomposites. The nonlinear stability behaviors of the sandwich beam having geometrically perfect/imperfect shapes have been studied when it is subjected to a compressive buckling load. The nanocomposite face sheets are made of epoxy reinforced by graphene oxide powders (GOPs). Also, the core has the shape of a honeycomb with regular configuration. Using finite element method based on a higher-order deformation beam element, the system of equations of motions have been solved to derive the stability curves. Several parameters such as face sheet thickness, core wall thickness, graphene oxide amount and boundary conditions have remarkable influences on stability curves of geometrically perfect/imperfect sandwich beams.

• 한국수산과학회지
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• 제33권1호
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• pp.55-59
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• 2000

무지개 송어의 뇌하수체 및 배양액에 존재하는 GTH II 농도를 측정하기 위해 Avidin- Biotin complex를 이용한 sandwich EIA 계을 개발했다. Protein A sepharose affinity chromatography을 통해서 얻어진 연어 GTH II의 rabbit IgG에 biotinylation시킨 것 (Biotin-salmon GTH II rabbit IgG)을 제2 항체로 사용하였고, Non-Biotin salmon GTH II rabbit IgG는 단지 protein A sepharose affinity chromatography에서 얻어진 IgG를 제 1 항체로 사용하였다. EIA는 sandwich법에 의해서 이루어졌으며, 효소반응 기질로는 TMB(3,3'5,5-tetramethylbenzidine)를 이용했으며, 반응후 450 nm의 흡광도에서 automatic microplate reader로 측정하였다. 그 결과, $0.12\;{\~}\;125\;ng/ml$의 범위에서 용량반응곡선을 얻었으며, 측정감도 (최소 검출량)는 거의 0.58 ng/ml 정도 였다. 그리고 뇌하수체 추출물 및 배양액 각각의 희석곡선은 GTH II 표존곡선과 일치 하였다. 또한 이러한 GTH II의 표준곡선는 뇌하수체내 다른 peptide hormone와는 교차반응을 거의 나타내지 않았다. Testosterone을 처리한 미성숙 무지개 송어의 뇌하수체 세포배양계를 이용하여 sGnRH에 의한 GTH II 분비량을 본 sandwich EIA계와 RIA계를 비교 조사한 결과, 거의 같은 분비량을 나타냈을 뿐만아니라 같은 분비 pattern을 나타냈다. 이러한 결과로부터 본 sandwich법 EIA계에 의해서 연어과 어류의 뇌하수체 추출물 및 뇌하수체 배양액 중의 GTH II 함량 및 분비량을 측정하는데 있어서 안정된 assay계라고 생각되어진다.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2000년도 추계학술대회 논문집
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• pp.73-78
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• 2000

This paper describes the effect of span-to-depth ratio, which describes aspect of cell formed with top diaphragm steel plate, on capacity in composite steel-concrete structure of sandwich system. The span-to-depth ratio \ulcorner load-carrying mechanism and load-distribution capacity of structure. Therefore, stress levels of members and load-resis\ulcorner of system vary according to span-depth ratio. In this study, numerical nonlinear analysis was performed to various ratio for two types(MA, MB) composite structure of sandwich system to analyze the influence of span-to-depth ratio or, behavior. The difference of load-carrying mechanism and stress of members results from analysis results, then bas\ulcorner differences, the effects of span-to-depth ratio on shear capacity, flexural capacity and load-resistance capacity were analyze effects on failure mode and ductility were briefly. As a results of this study, as span-to-depth ratio increases, \ulcorner bottom steel plate and concrete lower. This implies an increase in effective flexural and shear capacity. Therefore lo\ulcorner capacity of structure improves as span-to-depth ratio increases, Especially, the effect is greate in shear than flexural span-to-depth ratio increases, this difference between flexural and shear capacity may change failure mode and ductility. span-to-depth ratio increases capacity increases more than flexural capacity, we should expect that structural behavior mode gradually change from shear to flexural and ductility of structure gradually improves.

• 대한기계학회논문집B
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• 제38권4호
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• pp.347-355
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• 2014

전자장치들의 소형화 및 박형화가 됨에 따라 전자장치의 수명과 직결되는 열적문제가 중요해지고 있다. 열적문제를 해결하기위해 열확산과 단열을 통한 열적 안정성 연구가 필요하다. 허니컴 샌드위치 평판은 이방성의 열전도계수를 갖는다. 허니컴 샌드위치 평판이 적용된 시스템에 대해 온도분포와 열변형을 해석하기 위하여 열 및 탄성 물성치가 필요하다. 본 연구에서는 허니컴 코어의 크기, 두께 그리고 구성된 재료에 따라 허니컴 샌드위치 평판의 물성치가 변하기 때문에 허니컴 샌드위치 평판의 열전도계수, 열팽창계수, 탄성계수, 전단탄성계수, 푸아송비와 같은 열 및 탄성 물성치를 예측하였다.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2113-2120
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• 2018

Cross-reactive material 197 ($CRM_{197}$) is a non-toxic mutant of diphtheria toxin containing a single amino acid substitution of glycine 52 with glutamic acid. $CRM_{197}$ has been used as a carrier protein for poorly immunogenic polysaccharide antigens to improve immune responses. In this study, to develop a sandwich ELISA that can detect $CRM_{197}$ and $CRM_{197}$ conjugate vaccines, we generated a human anti-$CRM_{197}$ monoclonal antibody (mAb) 3F9 using a phage-displayed human synthetic Fab library and produced mouse anti-$CRM_{197}$ polyclonal antibody. The affinity ($K_D$) of 3F9 for $CRM_{197}$ was 3.55 nM, based on Bio-Layer interferometry, and it bound specifically to the B fragment of $CRM_{197}$. The sandwich ELISA was carried out using 3F9 as a capture antibody and the mouse polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA was <1 ng/ml $CRM_{197}$. In addition, the 3F9 antibody bound to the $CRM_{197}$-polysaccharide conjugates tested in a dose-dependent manner. This ELISA system will be useful for the quantification and characterization of $CRM_{197}$ and $CRM_{197}$ conjugate vaccines. To our knowledge, this study is the first to generate a human monoclonal antibody against $CRM_{197}$ and to develop a sandwich ELISA for $CRM_{197}$ conjugate vaccines.

• 한국재난정보학회 논문집
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• 제15권1호
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• pp.98-108
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• 2019

연구목적:본 연구는 화재 시 샌드위치패널 구조 창고의 벽 천장접합부 방호를 위한 스프링클러 성능확인 실험을 목적으로 한다. 연구방법: 현장조사결과를 기반으로 창고의 공간규격과 가연물을 선정하고 벽 코너 및 헤드 직하부에서의 방호실험을 수행하고 결과를 분석하였다. 연구결과:벽 코너 화재 시나리오에 대해서 K-80 폐쇄형 스프링클러헤드의 작동으로 샌드위치 패널의 착화 및 접합부 손상이 방지됨을 확인하였다. 결론:샌드위치패널 심재로의 착화 및 연소방지를 통한 화세제어로 이를 가정한 소방대의 소화 및 구조활동을 위한 표준진압대책이 마련되어야 한다.

• 한국화재소방학회논문지
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• 제31권5호
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• pp.37-43
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• 2017

국내 샌드위치패널 건축물은 공장 창고시설의 벽 지붕에 널리 사용된다. 공장 창고시설은 용도특성상 화재하중이 높고 화재확산이 신속히 진행되어 대형화재로 이어지며 재료특성 상 벽 지붕이 무너져 인명피해 및 재산피해가 증가하고 있는 추세이다. 이를 위하여 본 연구에서는 샌드위치패널 스프링클러설비의 국내 외 관련기준을 검토하였다. 현장조사결과 가연물이 벽면에 밀착되어있는 경우가 있어 벽면으로부터 10 cm, 60 cm, 120 cm 이격하여 벽면보호여부를 확인하는 실험을 수행하였다. 화재실험결과, 방수압력 0.1 MPa, 방수량 K-80의 헤드 설치 시, 샌드위치패널착화방지를 확인하였다. 또한 가연물이 벽면으로부터 60 cm일 때 샌드위치패널벽면의 최고온도가 $525^{\circ}C$, 120 cm에서는 최고온도가 $276^{\circ}C$로 측정되었다. 한편, 국외소방연구기관의 EPS 샌드위치패널연구에 따르면 발화점이 $450^{\circ}C$인 것을 감안할 때, 패널 벽면에서 가연물거리는 120 cm 이상 이격하면 착화방지가 가능함을 확인하였다.