• Mass Spectrometry Letters
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• v.6 no.2
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• pp.27-30
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• 2015

This article reviews the fundamentals of sample preparation used in matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). MALDI is a soft ionization method used to generate analyte ions in their intact forms, which are then detected in MS analysis. MALDI-MS boasts fast analysis times and easy-to-use operation. The disadvantages of MALDI-MS include the occurrence of matrix-associated peaks and inhomogeneous distribution of analyte within the matrix. To overcome the disadvantages of MALDI-MS, various efforts have been directed such as using different matrices, novel matrix systems, various additives, and different sample preparation methods. These various efforts will be discussed in detail. This article will benefit those who would like to obtain basic knowledge of MALDI sample preparation and those who would like to use MALDI-MS in their chemical analyses.

• Journal of Forest and Environmental Science
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• v.33 no.4
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• pp.295-304
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• 2017

This study compared and analyzed the construction of a land use change matrix for the Intergovernmental Panel on Climate Change's (IPCC) land use, land use change, and forestry area (LULUCF). We used National Forest Inventory (NFI) permanent sample plots (with a sample intensity of 4 km) and permanent sample plots with 500 m sampling intensity. The land use change matrix was formed using the point sampling method, Level-2 Land Cover Maps, and forest aerial photographs (3rd and 4th series). The land use change matrix using the land cover map indicated that the annual change in area was the highest for forests and cropland; the cropland area decreased over time. We evaluated the uncertainty of the land use change matrix. Our results indicated that the forest land use, which had the most sampling, had the lowest uncertainty, while the grassland and wetlands had the highest uncertainty and the least sampling. The uncertainty was higher for the 4 km sampling intensity than for the 500 m sampling intensity, which indicates the importance of selecting the appropriate sample size when constructing a national land use change matrix.

• Journal of Korean Society for Quality Management
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• v.29 no.1
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• pp.1-10
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• 2001

We consider the problem of detecting special variations in multivariate $T^2$-control chart when two or more multivariate outliers are present. Since a multivariate outlier may reflect slippage in mean, variance, or correlation, it can distort the sample mean vector and sample covariance matrix. Damaged sample mean vector and sample covariance matrix have difficulty in examining special variations clearly, An alternative to detection outliers or special variations is to use robust estimators of mean vector and covariance matrix that are less sensitive to extreme observations than are the standard estimators $\bar{x}$ and $\textbf{S}$. We applied popular minimum volume ellipsoid(MVE) and minimum covariance determinant(MCD) method to estimate mean vector and covariance matrix and compared its results with standard $T^2$-control chart using simulated multivariate data with outliers. We found that the modified $T^2$-control chart based on the above robust methods were more effective in detecting special variations clearly than the standard $T^2$-control chart.

• Journal of Korean Society of Transportation
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• v.26 no.6
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• pp.123-132
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• 2008

Dynamic origin-destination (OD) trip matrix has been widely used for transportation fields such as dynamic traffic assignment, traffic operation and travel demand management, which needs precise OD trip matrix to be collected. This paper presents a quasi-dynamic OD matrix estimation model and applies it to real road network for collecting the dynamic OD matrix. The estimation model combined with dynamic traffic assignment program, DYNASMART-P, is based on GPS embedded in PDA, which developed for collecting sample dynamic OD matrix. The sample OD matrix should be expanded by the value of optimal sampling ratio calculated from minimization program. From application to real network of Jeju, we confirm that the model and its algorithm produce a reasonable solution.

• Macromolecular Research
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• v.16 no.2
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• pp.108-112
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• 2008

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis of nonpolar polymeric materials is affected by the sample preparation as well as the matrix and cationizing agent. This study examined the influence of silver salt types on the MALDI analysis of polybutadiene (PB). Silver trifluoroacetate (AgTFA), silver benzoate (AgBz), silver nitrate ($AgNO_3$), and silver p-toluenesulfonate (AgTS) were used as the silver salts to compare the MALDI mass spectra of PB. The mixture solution of PB and 2,5-dihydroxybenzoic acid (DHB), as a matrix dissolved in THF, was spotted on the sample plate and dried. A droplet of the aqueous silver salt solution was placed onto the mixture. The mass spectrum with AgBz showed the clear $[M+Ag]^+$ ion distribution of PB while the mass spectrum with AgTFA did not show $[M+Ag]^+$ ions but only silver cluster ions. The mass spectra with $AgNO_3$ and AgTS did not show a clear $[M+Ag]^+$ ion distribution. The difference in the formation of $[M+Ag]^+$ ions of PB depending on the silver salts was attributed to the silver cation transfer reaction between the silver salt and the matrix (DHB). The mass spectrum showed a clear $[M+Ag]^+$ ion distribution of PB when the conjugate acid of the silver salt was less acidic than the matrix.

• The Korean Journal of Applied Statistics
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• v.26 no.5
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• pp.747-758
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• 2013

The covariance matrix is important in multivariate statistical analysis and a sample covariance matrix is used as an estimator of the covariance matrix. High dimensional data has a larger dimension than the sample size; therefore, the sample covariance matrix may not be suitable since it is known to perform poorly and event not invertible. A number of covariance matrix estimators have been recently proposed with three different approaches of shrinkage, thresholding, and modified Cholesky decomposition. We compare the performance of these newly proposed estimators in various situations.

• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2293-2298
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• 2010

Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.2
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• pp.301-312
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• 1996

This study was performed to search a optimal analyzing method of cadmium in whole-blood. Cadmium was determined by graphite furnace atomic absorption spectrometry(GFAAS). We investigated the effect of ashing temperature on the absorbance of cadmium in a simple dilution(ten-fold) method with triton X-100 and matrix modifier methods treated with $NH_4H_2PO_4$(1 and 3%) and $Pd(NO_3)_2$(0.00l and 0.005%) as matrix modifier. We also compared the reported reference values of standard blood with values resulted from optimal analyzing conditions of this study. In case of a simple dilution method, when ashing temperature was set at $450^{\circ}C$, the absorbance of sample and background were $0.334{\pm}0.012$ and $1.382{\pm}0.245$, respectively. Background level was higher than the value(0.8) that can be corrected by $D_2$ background correction method. As ashing temperature was rised to $500^{\circ}C$, the absorbance of sample and background were $0.178{\pm}0.008$ and $0.711{\pm}0.223$ respectively. The higher ashing temperature($450^{\circ}C-650^{\circ}C$) was, the lower the absorbance of sample was. In case of a matrix modifier method with $NH_4H_2PO_4$(1 and 3%), when ashing temperature was rised from $500^{\circ}C$ to $650^{\circ}C$, the absorbance of sample slightly changed. The absorbances of sample at $600^{\circ}C$ were $0.230{\pm}0.017$ and $0.137{\pm}0.012$, respectively. These values were larger than that of simple dilution method. But the absorbance of background was higher than the level that can be corrected by $D_2$ method. In case of a matrix modifier method with $Pd(NO_3)_2$(0.001 and 0.005%), the absorbance of sample and background were higher than those of other methods and were stable and reproducible. When ashing temperature was over $550^{\circ}C$, the absorbance of sample was significantly decreased. In case of 0.005% $Pd(NO_3)_2$ carbon residue remained in graphite tube affected the absorbance of sample and background. From these results, We propose that in case of a simple dilution(ten-fold) method with triton X-100 ashing temperature must be maintained below $400^{\circ}C$. In order to diminish the absorbance of background, the alternative method is attenuation of injection volume or multiplication of dilution ratio. We recommend $Pd(NO_3)_2$ than $NH_4H_2PO_4$ as a matrix modifier. In case of a matrix modifier method with $Pd(NO_3)_2$ ashing temperature might be maintained below $550^{\circ}C$.

• Journal of the Optical Society of Korea
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• v.16 no.4
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• pp.443-448
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• 2012

A polarimetric experimental method was developed to determine the Jones matrix elements of transparent optical materials without sign ambiguity. A set of polarization dependent transmittance data of the samples was measured with polarizer - sample - analyzer system and another set of data was measured with polarizer - sample - quarter-wave plate - analyzer. Two data sets were compared and mathematically analyzed to obtain the correct signs of the elements of the matrix. The Jones matrix elements of a quarter-wave plate were determined to check the validity of the method. The experimentally obtained matrix elements of the quarter-wave plate were consistent with the theoretical expectations. The same method was applied to obtain the Jones matrix elements of a twisted nematic liquid crystal panel.

• Journal of the Korea Institute of Military Science and Technology
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• v.16 no.3
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• pp.382-389
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• 2013

In anti-jamming GPS receiver, adaptive signal processing techniques in which the radiation pattern of adaptive array antenna of elements may be adaptively changed used to reject interference, clutter, and jamming signals. In this paper, I describes adaptive signal processing technique using the sample matrix inversion(SMI) algorithm. This adaptive signal processing technique can be applied effectively to wideband/narrowband anti-jamming GPS receiver because it does not consider the satellite signal directions and GPS signal power level exists below the thermal noise. I also analyzed the effects of covariance matrix sample size and diagonal loading technique on the system performance of five-element circular array antenna. To attain near optimum performance, more samples required for calculation covariance matrix. Diagonal loading technique reduces the system nulling capability against low-power jamming signals, but this technique improves robustness of adaptive array antenna.