• Archives of Pharmacal Research
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• 제17권5호
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• pp.360-363
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• 1994

Column--swtiching technique with a reversed-phase high performance liquid chromatographic method has been developed for the routine analysis of radioiodinated insulin and its degadation products in biological fluids. The diluted biological samples were loaded onto a precolumn packed with LiChrosorb RP-8 $(25-40{\;}{\mu}m)$ using 0.1% trifuoroacetic acid (TFA) in water as a washing solvent. After valve switching, the concentrated insulins were eluted in the back-flush mode and separated by a W-Porex $C_{18}$ column with a gradient of 0.1% TFA in water and 0.1% TFA in acetonitrile as the mobile phase. The method showed good precision, accuracy and speed with the detection limit of 20 pg/ml. Total analysis time per sample was about 40 min and the coefficients of variation were less than 8, 2%.

• 한국동물위생학회지
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• 제30권1호
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• pp.77-84
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• 2007

The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP l134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.

• Nuclear Engineering and Technology
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• 제10권1호
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• pp.13-25
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• 1978

1970년 12월에서 1976년 12월 사이의 6년간 고리 원자력발전소 주변 환경에서 채취한 시료에 대하여 환경방사능을 측정조사하였다. 1976년에 채취한 공기미진에서 진알파 방사능과 1970년 12월에서 1976년 12월 사이에 채취한 시료인 토양, 물, 공기미진, 낙진, 솔잎, 빗물과 여런 식품시료에 페하여 전베타 방사능을 측정하였다. 시금치, 배추, 보리, 쌀, 뱀장어, 조기, 미역, 파해 및 우유 중에 함유된 스트론튬-90및 세시움-137 방사능은 방사화학적으로 분석하여 측정하였고 정수, 하천수, 바닷물 중의 트리튬도 전해법으로 농축하여 그 방사능을 측정하였다.

• Bulletin of the Korean Chemical Society
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• 제18권9호
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• pp.958-961
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• 1997

The compatibility and the surface structure of blends of poly(styrene-co-acrylonitrile) (SAN) with either poly(methyl methacrylate) (PMMA) or hydrolyzed PMMA (H-PMMA) were studied in terms of film thickness, interaction, and surface free energy difference on the basis of X-ray photoelectron spectroscopy (XPS), attenuated total reflection Fourier transform IR spectroscopy and atomic force microscopy. The XPS measurement showed that the surface enrichment of (PMMA/SAN) blends with different AN contents of SAN and with different carboxyl acid contents of PMMA was dependent on the molecular interaction, the surface free energy difference between components and the sample preparation history. It was found that the compatibility of H-PMMA and SAN was reduced with increasing carboxyl acid content of PMMA.

• 대한수의학회지
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• 제38권4호
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• pp.763-770
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• 1998

Salmonella typhimurium is a causitive agent of diarrhea, fever, gastroenteritis, septicemia and sudden death in piglet. The currently used methods such as IFA, ELISA, DNA hybridization assay is needed a long-time and difficult to detect the organism in carrier animal or contaminated sample with other agents. However, it is important to detect rapidly and sensitively S typhimurium in piglet with other infectious pathogens to minimize an economic loss. Two sets of PCR primer, rfbJ forward primer(5'-AGAATATGTAATTGTCAG-3') and reverse primer(5'-TAACCGTTTCAGTAGTTC-3') were designed to amplify a 882 by fragment of Salmonella serovar type B gene. The target genomic DNA for PCR was extracted from the cultivated materials with various enrichment periods in a nonselective enrichment agar and broth with clinical specimens. The PCR is carried out here made it possible to detect the gene from two hours. Also, the amplified fragment with PCR was cloned into pGEM-T vector and digested with restrict enzyme, and sequenced for the identification of Salmonella serotype B rfbJ gene. Duplicated cultivation agar-broth followed by PCR were performed to develop a rapid and sensitive detection of S typhlmurium based on serovar type. This duplicated cultivation-PCR method provides a sensitive and rapid diagnostic tool to detect Salmonella from infected piglet with improved sensitivity.

• 공업화학
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• 제9권1호
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• pp.121-128
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• 1998

삼중수소를 함유하는 수소동위원소 시설의 운용이나 취급 또는 핵융합소재의 관리기술분야에서는 기체크로마토그래피 분리법이 중요한 분석 및 농축기술중의 하나로 활용되고 있다. 수소, 중수소 및 삼중수소의 수소동위원소 혼합기체시료를 상용 기체크로마토그래피를 사용하여 분리하였다. 기체시료는 특별히 제작된 진공-시료주입장치를 통해 액체질소온도의 분위기로 유지된 분리컬럼을 통과하는 비활성 캐리어 기체의 흐름을 주입하였다. 10% 함량의 염화망간으로 부분-비활성화한 산화알루미늄 고정상에서 수소동위원소들이 완전히 분리됨을 확인할 수 있었다. 그리고 이성질체피크의 억제와 분리 용리시간이 단축되어 실제 수소동위원소의 분리 및 농축기술에 유용하게 적용할 수 있는 비교적 우수한 분리조건을 얻었다.

• KSBB Journal
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• 제28권5호
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• pp.295-302
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• 2013

Indigo is utilized in various industries including textile dyeing, cosmetics, printing and medicinal products and its reduced form, leuco-indigo, is mainly used in these process. Chemical reducing agent (sodium dithionite, sodium sulfide, etc.) is preferred to use for the formation of leucoindigo in industry. In traditional indigo fermentation process, microorganisms can participate in the reduction of indigo and thus it has been known to reduce environmental pollution and noxious byproducts. However, in fermentation method using microorganisms it is difficult to standardize large scale production process due to low yield and reproducibility. In this study, we attempted to develop the indigo reduction process using microbial flora which was isolated from naturally fermented indigo vat or deduced by metagenomic approach. From the results of library analyses of PCR-amplified 16S rRNA genes from the traditional indigo fermentation vat sample (metagenome), it was confirmed that Alkalibacteriums (71%) was distinctly dominant in population. Some strains were identified after confirming that they become pure culture in nutrient media modified slightly. Four strains were separated in this process and each strain showed obvious reducing ability toward indigo in dyeing test. It is expected that the analyzed results will provide important data for standardizing the natural fermentation of indigo and investigating the mechanism of indigo reduction.

• 공학교육연구
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• 제17권3호
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• pp.51-58
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• 2014

The main objective of this research lies in estimating how much importance the educators of professional general education and MSC curriculum put on assessment, and, thereafter, take what support should be provided for the efficient assessment of education. The subjects of this research are educators of professional general education and MSC curriculum in the engineering education enrichment program at the 58 universities out of 72 universities where the accreditation for engineering education is implemented. Accordingly questionnaires were distributed to a total of 58 universities among which 136 questionnaires were collected. The data analysis methods, mean and response sample T test, were used in this research. The following conclusions were drawn from the results of this research. First, tile mean of importance and performance in the 'assessment activities' were relatively high. However, in tile assessment, the assessment performance was relatively lower than the assessment importance. Second, the results of the Importance-Performance Matrix in the 'assessment activities' was analyzed by two ways. First, Analyzed with the scale mean, The result means that Keep up the Good Work (KGW) sector included all factors. Second, Analyzed with the actual mean, The result means that KGW sector included 5 factors A, J, B, D, C. Possible Overkill(PO) sector included factors C, I. Low Priority(LP) sector included 4 factors K, E, H, L. Concentrate Here (CH) sector included factor G.

• 대한화학회지
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• 제27권5호
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• pp.345-352
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• 1983

Diphenylcarbazide를 흡착시킨 큰 그물구조 수지를 사용하여 컬럼 조작에 의해 0.1M 황산수용액에서 크롬(Ⅵ)이온을 선택적으로 농축하였다. 시료수의 액성과 유속을 조절하여 각종 수지에 대해 크롬(Ⅵ)이온 추출실험을 한 결과 Diaion HP-20이 가장 큰 돌파교환용량을 나타내었다. Diaion HP-20에 대해 크롬(Ⅵ)이온의 돌파점은 흡착된 DPC의 농도에 비례하였으며 크롬(Ⅵ)이온의 농도가 진할 때가 묽을 때 보다 높은 돌파교환용량을 나타냈다. 또한 일반 환경수 속에 공존되기 쉬운 각종 중금속 이온들에 대한 크롬(Ⅵ)이온의 농축에 미치는 영향을 조사한 결과 10ppm 크롬(Ⅵ)이온에 대해 철(Ⅲ)이온을 제외한 모든 금속 이온들은 100배까지 공존하여도 영향을 미치지 않았으며 철(Ⅲ)이온은 10배까지 방해되지 않았다. 크롬(Ⅵ)이온 추출에 이용된 겔 입자는 메탄올로서 쉽고도 효과적으로 재생되므로 반복 사용할 수 있다.

• 대한화학회지
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• 제38권7호
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• pp.502-508
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• 1994

염의 농도가 큰 시료용액중에 미량으로 함유되어 있는 U(VI)과 Th(VI)을 액상크로마토그래피로 동시에 분리, 정량하였다. 약 2ml의 시료를 pH5.5의 0.11M ${\alpha}$-HIBA 용리액과 함께 C_{18}$ 역상농축컬럼에 통과시켜 U(VI)과 Th(VI)을 농축시켰으며, 농축된 이들 원소들을 다시 비활성 C_{18}$ 역상분리컬럼에서 pH3.0의 0.17M ${\alpha}$-HIBA/0.0038 M 1-pentanesulfonate 용리액으로 각각 분리하였다. 분리된 이들 원소들은 Arsenazo III를 사용하는 postcolumn반응으로 검출되었으며, 검출한계는 두 원서 모두 1ppb이었다.