• 한국환경과학회지
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• 제4권4호
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• pp.317-321
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• 1995

ABA와 SA는 같은 조건하에서 기공 닫힘에 대하여 다른 효과를 나타냈다. 활짝 열린 기공에 1 mM Salicylic acid(SA)를 첨가 하였을 때, 열린 기공 크기의 22%가 감소하였다. 그러나 1mM ABA는 열린 기공 크기를 73%나 감소시켰다. SA용액의 광흡수 스펙트라 조가 셜과 SA가 1시간 내에 분해 되었다. 따라서, 분리표와 배양 동안에 SA 용액을 20분 마다 갈아 주었다. 그 결과 심지어 10$\mu$M SA도 기공 닫힘을 크게 유도하였으며, 기공 닫힘에 대한 SA의 효과는 pH에 크게 으존적이었다. 1mM SA에 의하여 유도된 기공크기의 감소는 낮은 pH(pH 7.2, 5%; pH 6.2, 40%; pH 5.2, 78%)에서 가장 효과적이었다. 따라서, SA를 배양액에 있는 분리 표피에 적절히 처리하면 기공 닫힘에 대한 효과는 ABA와 거의 같다.

• BMB Reports
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• 제31권1호
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• pp.70-76
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• 1998

The final step in ethylene biosynthesis is catalyzed by the enzyme 1-aminocyclopropane-1-carboxylate (ACC) oxidase. ACC oxidase was extracted from mung bean hypocotyls and its biochemical characteristics were determined. In vitro ACC oxidase activity required ascorbate and $Fe^{2+}$, and was enhanced by sodium bicarbonate. Maximum specific activity (approximately 20 nl ethylene $h^{-1}$ mg $protein^{-1}$) was obtained in an assay medium containing 100 mM MOPS (pH 7.5), $25\;{\mu}M$ $FeSO_4$, 6 mM sodium ascorbate, 1 mM ACC, 5 mM sodium bicarbonate and 10% glycerol. The apparent $K_m$ for ACC was $80{\pm}3\;{\mu}M$. Pretreating mung bean hypocotyls with ethylene increased in vitro ACC oxidase activity twofold. ACC oxidase activity was strongly inhibited by metal ions such as $Co^{2+}$, $Cu^{2+}$, $Zn^{2+}$, and $Mn^{2+}$, and by salicylic acid. Inactivation of ACC oxidase by salicylic acid could be overcome by increasing the $Fe^{2+}$ concentration of the assay medium. The possible mode of inhibition of ACC oxidase activity by salicylic acid is discussed.

• Molecules and Cells
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• 제22권2호
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• pp.233-238
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• 2006

Endogenous salicylic acid (SA) and its predominant conjugates, SA 2-O-${\beta}$-D-glucoside (SAG) and the glucose ester of SA (SGE), increase dramatically during plant defense responses. Here I report the isolation and characterization of an Arabidopsis thaliana UDP-glucose:SA glucosyltransferase1 (AtSGT1) gene using a tobacco SGT gene previously reported, whose product catalyzes the formation of both SAG and SGE. The recombinant AtSGT1 protein had significant activities with SA and benzoic acid, and synthesized SAG and SGE. Northern blot analysis showed that AtSGT1 was rapidly induced both by exogenous SA and infection with the bacterial pathogen Pseudomonas syringae, indicating that pathogen-inducible AtSGT1 expression is an early disease response and may be involved in the accumulation of glucosyl SA during pathogenesis.

• 대한화장품학회지
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• 제19권1호
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• pp.77-84
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• 1993

본 연구는 여드름의 원인균인 Propionibacterium acnes ATCC 6919에 대해 항균력을 보이는 오배자의 유효 성분을 규명하고자 수행되었다. 오배자의 95%에탄올 추출물, 노르말헥산 추출물, 노르말헥산 추출물 중 검화성 물질의 이 균에 대한 최소 생육 저지 농도는 각각 20${\mu}\ell$/ml, 10${\mu}\ell$/ml, 5${\mu}\ell$/ml이었다. 그 검화성 물질로부터 2종의 항균 성분을 분리하였으며 이들은 13C-NMR, 1H-NMR, MS, IR, UV 스펙트라에 근거하여 각각 6-pentadec-8-enyl salicylic acid과 6-pentadecyl salicylic acid로 동정되었다. 이들은 공히 1${\mu}\ell$/ml 이하의 농도에서 P. acnes 의 생육을 저지하였다.

• 한국잡초학회지
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• 제18권4호
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• pp.348-355
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• 1998

고소득 작물인 인삼의 생산비 절감 및 고품질화를 위한 새로운 재배법을 확립하기 위하여 Allelopathic 식물이 함유하고 있는 제초활성물질 탐색 동정한 결과는 다음과 같다. 12종류의 표준품을 이용하여 호밀과 귀리의 추출물에 대한 Allelopathy 물질을 분석한 결과, 두 추출물간에 함유하는 물질의 차이를 보였으나 호밀에서는 benzoic acids계열인 salicylic acid(8.34mg/g), 귀리에서는 flavonone glycosides계열인 naringin(7.50mg/g)이 가장 많이 함유되어 있음을 알 수 있었으며, 동정된 표준품을 이용하여 $10^{-3}M$과 $10^{-4}M$에서 명아주 종자에 대한 발아율, 평균 발아 일수 및 발아세를 조사한 결과 대조구에 비하여 통계적으로 낮은 값을 보여 salicylic acid, naringin를 비롯하여 이러한 물질들이 Allelopathy를 나타내는 성분일 것으로 생각되었다.

• 분석과학
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• 제8권3호
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• pp.305-311
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• 1995

Salicylic acid($C_6H_4(OH)COOH=H_2A$)와 전이금속인 Fe(III) 사이에 형성되는 착물의 안정도상수를 $20^{\circ}C$, 0.1M $KNO_3$ 수용액 중에서 자외-가시선 흡수광도법 및 pH 전위차적정법으로 측정하여 문헌값($logK_f=16.48$)과 비교하였다. $H_2A$의 $pK_{a1}$과 $pK_{a2}$는 $2.92{\pm}0.08$ 및 $12.90{\pm}0.13$이며, Fe(III)-$H_2A$의 1:1 착물의 $logK_f$는 $20^{\circ}C$에서 $11.88{\pm}0.12$였다.

• 대한화학회지
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• 제46권2호
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• pp.99-116
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• 2002

Thiazolylazo 화합물과 barbituric산, uracil, thiouracil, citrazinic산 chromotrotropic산, gallict산, pyrogallol 그리고 salicylic산의 Co(II), Ni(II) 그리고 Cu(II) 착화합물의 제조하였고, $^1H$ NMR, IR, 그리고 전기 흡수 스펙트럼에 대한 pHdudgid에 의하여 규명하였다. 이온화형태, 전기적 전이 그리고 해리상수에 대하여 설명하였다. 구리 착화합물은 등방형 ESR스펙트럼이었으며 자기적으로 궤도 기여를 갖는 묽은 성질이었다. 상세한 DTA데이타를 얻었고, 그 결과를 토론하였다.

• 한국자원식물학회지
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• 제11권2호
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• pp.195-201
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• 1998

Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required $\beta$-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases.

• Mycobiology
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• 제30권2호
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• pp.88-92
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• 2002

Rehmannia glutinosa is a perennial medicinal plant belonging to the family Scrophulariaceae with more than 300 species known in the world, especially in temperate regions. Its roots have been used widely in Korea for medicinal purposes. However, it is commonly infected by various pathogens during storage, causing great damage to the roots, and impedes the intensive farming of the crop. Therefore, an attempt has been made to isolate and screen a resistance gene against the pathogen Fusarium oxysporum using differential display. We treated salicylic acid(SA), and isolated a resistance gene that responds to SA. As a result, we found that SA was involved in plant defense mechanism in pathogenicity tests with SA treated and non-treted plants, and we isolated a partial PR-la gene through differential display polymerase chain reaction(DD-PCR) method.

• International Journal of Computer Science & Network Security
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• 제22권6호
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• pp.91-96
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• 2022

The photocatalytic degradation of salicylic acid takes place in several stages involving coupled phenomena, such as the transport of molecules and the chemical reaction. The systems of transport equations and the photocatalytic reaction are numerically solved using COMSOL Mutiphysics (CM) simulation software. CM will make it possible to couple the phenomena of flow, the transport of pollutants (salicylic acid) by convection and diffusion, and the chemical reaction to the catalytic area (bauxite or TiO2 doped by nanoparticles). The simulation of the conversion rate allows to correctly fit the experimental results. The temporal simulation shows that the reaction reaches equilibrium after a transitional stage lasting over one minute. The outcomes of the study highlight the importance of diffusion in the boundary layer and the usefulness of injecting micro-agitation into the microchannel flow. Under such conditions, salicylic acid degrades completely.