• Archives of Pharmacal Research
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• v.14 no.3
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• pp.231-236
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• 1991

The high affinity binding sites for angiotensin II were solubilized from rat liver membranes by treatment with CHAPS. The binding protein was also partially purified by angiotensin III inhibitor-coupled Affi-gel affinity chromatography. Binding to the intact membrances as well as to the solubilized preparation was specific and saturable. According to the Scatchard plot, the membrane preparations exhibited a single class of high affinity binding sites with a Kd OF 0.71 nM. The solubilized preparation also showed the presence of a single class of bindings sites with less affinity (Kd of 14 nM). Meanwhile the competition studies using angiotensin II analogues represented two separate binding sites for angiotensin II and single binding site for antagonist. These latter findings were correlated to the results provided by Garrison's research group. More works are needed to clarify this discrepancy.

• The Korean Journal of Zoology
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• v.37 no.2
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• pp.274-280
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• 1994

담배나방(Heliothis assulta) 혈림프 항세균성 단백질의 특성을 알아보기 위해 종령 유충의 혈강으로 고라구연us thuringiens논 균을 주입하였다. 혈림프의 항세근적 활성은 주입후 48시간 경과군에서 가장 높았으며, E. coli와 풍 thuringiensis에 대하여 항세균성을 보이는 두 개의 단백질(G-1 및 G-11)들을 cation exchange chromatography와 gel filtration을 통해 정제하였다 49KD와 46KB의 두 subunit로 구성된 G-1은 높은 glvcine(17 13 mole %) 및 alanine(13.88 mole %) 함량을 보였으며, 14KD의 단일 pclvpeptide인 G-11에서는 histidine(16 14 mole %)과 glvclne(14.27 mole %)의 함량이 높아, 이러한 높은 glvcine 함량에 있어서 곤충에서 나타나는 다른 항세균성 단백질과 공통점을 보인다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1246-1251
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• 1997

When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh. A was dependent on $Ca^{2+}$, and independent on $Mg^{2+}$ or calmodulin. When adding trigger peptidase(TPase) inhibitor, antipain, no inhibition of phosphory was observed. Also, by adding the trysin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.

• Honam Mathematical Journal
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• v.43 no.3
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• pp.373-383
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• 2021

In this study, a numerical method deals with the Chebyshev wavelet collocation and Adomian decomposition methods are proposed for solving Korteweg-de Vries equation. Integration of the Chebyshev wavelets operational matrices is derived. This problem is reduced to a system of non-linear algebraic equations by using their operational matrix. Thus, it becomes easier to solve KdV problem. The error estimation for the Chebyshev wavelet collocation method and ADM is investigated. The proposed method's validity and accuracy are demonstrated by numerical results. When the exact and approximate solutions are compared, for non-linear or linear partial differential equations, the Chebyshev wavelet collocation method is shown to be acceptable, efficient and accurate.

• Clinical and Experimental Pediatrics
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• v.50 no.1
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• pp.52-55
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• 2007

Purpose : Hyperbilirubinemia, jaundice and gallbladder hydrops are unusual manifestations of Kawasaki disease (KD). In this case, abdominal pain, anorexia and abdominal distension may follow eventfully. We reviewed the clinical and laboratory data to investigate the causative factors of hyperbilirubinemia in patients with KD. Methods : Two-hundred eighty two children diagnosed and hospitalized with KD were identified by searching patients' charts. Cases were included in the study if diagnosed between January 1997 and December 2004. We reviewed clinical data, age, sex, duration from the onset of fever to admission and duration from start of treatment to defervescence. Clinical and laboratory data were compared between normal (A) and hyperbilirubinemia (B) groups. Results : Thirteen patients (4.6 percent) showed hyperbilirubinemia. In the hyperbilirubinemia group, age and initial ALT value were higher than group A (P=0.003, 0.018 respectively). Duration from the onset of fever to admission and age were relative risk factors in hyperbilirubinemia (P=0.007, 0.003 respectively) in patients with KD. Conclusion : Shorter duration from the onset of fever to admission and older age group were relative risk factors of hyperbilirubinemia in patients with KD.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.289-299
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• 1993

The polypeptide patterns of cellular and follicular components were analysed by SDS-PAGE and two dimensional(2-D)electrophoresis combined with isoelectric focusing (IEF) to establish protein profiles in each of the components in porcine follicles. Oocyte-cumulus complexes were cultured in M16+FCS+Gn at 39 in an atmosphere of 5% CO$_2$, in air for 35 h. At the end of the culture, the zona-free oocyte, ZP alone and cumulus cells were prepared and analysed either on 10% SDS-PAGE for the protein profile at the first dimensional gel or 2-D protein pattern. The amounts of each samples were determined for the visualization with Coomasie brilliant blue (CBB) or silver staining, thus giving useful information for the identification of specific proteins in the components or appropriate amount of samples for proper visualization. Oocyte showed 25 and 114 kd major protein band. Other minor components were additionally visualized with CBB on the same gel after silver staining procedure. Cumulus cells also showed specific proteins which is not present in the oocytes. The number of cumulus cell was proper to give major bands with CBB and additional minor bands with silver staining. To establish the degree of contamination from the remnant of the corona radiata to the ZP, zonae were differently prepared or analysed by SDS-PAGE.The preparation of the ZP in this study did not showed any contamination judged by the protein profile of the components. Also follicular fluid showed its specific protein profile without any significant differences among the different sizes of follicles. The established protein profile of each follicular component should be helpful for the identification and elimination of contaminated components, i. e., antigen preparation or immunological studies. The results also suggest that the preparation of each components in the study was appropriate and can be used for a further sensitive biochemical analysis in mammalian oocytes and early embryos.

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.7-13
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• 1989

This study was executed to investigate characterization of physiological genetic in Coriolus versicolor, basidiomycetes. The optimal media for mycelia culture were PDA, CVT-I and MES as solid media, 927 and CVT-III were good as liquid media, respectively. The optimal condition for mycelial culture was pH 5.6 and $25-30^{\circ}C$. Electrophoretic isozymes and protein patterns from mycelia identified very similar in 16001 and KD88001 strain, but the other species were very diffrent in band patterns. Especially, pattern of esterase seemed to be valuable tool as taxonomic techniques for indentifying species of Coriolus versicolor. Fruiting body was cultivated with artificial logs cultivation method; 16001 and KD88001 were very similar to fruiting body shapes and colours but 16001 and CVT-80 were different in their shapes, colours and making primordia. Therefore, 16001 and KD88001 were assumed to the same strain, but 16001, 16002 and CVT-80 had the different genetic background.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.18-24
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• 1999

Kinesin has been discovered in Saccharomyces cerevisiae, Aspergillus nidulans, and Drosophila melanogaster and it has major roles in the movemenl of chromosomes and separation of spindle poles. In this study, a gene encoding kinesin heavy chain in Schizosaccharo~n)~ces pombe was cloned by using the polymerase chain reaction with degenerated primcrs corresponding to highly conserved regions of the kinesin heavy chain motor domain. The kinesin gene in S pombe contains an open reading frame of 2496 base pairs and encodes a kinesin prolein of 832 amino acids with a molecular weight of 96 kd. From thc comparison of the predictcd amino acids of the newly cloned kinesin, the kinesin in S. pornbe belongs to the C-terminal motor subfamily of kincsin-related protein.

• Korean Journal of Human Ecology
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• v.14 no.3
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• pp.485-489
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• 2005

The purpose of this study is to investigate changes of isoflavone and nutrients during germination of soybean varieties and to determine the optimal germination condition for soybean processing. After 5 days' germination, water content of the soybeans increased in the order of Poongsannamoolkong> Sinpaldal#2> Black#1> Jinpumkong. Ash content was higher in the order of Poongsannamoolkong> Sinpaldal#2> Jinpumkong> black#1 soybean. All varieties of the soybean samples increased in ash content during germination. Crude fat content was higher in the order of Poongsannamoolkong> Black#1> Jinpumkong> Shinpaldalkong. Crude protein content was higher in the order of Shinpaldalkong> Black#1> Jinpumkong> Poongsannamoolkong. Polypeptide subunits separated from the SDS-PAGE of the entire proteins were showing comparatively high level in the range of 2-16KD in Jinpumkong and 2-21KD in Poongsannamoolkong till the first day of germination, 2-25KD in Sinpaldal#2 till the second day, and 6-27KD in Black#l till the third day. Changes of total isoflavone contents during germination showed different patterns in soybean varieties: Jinpumkong and Black#1 had the highest total isoflavone content on the fourth day of germination, Poongsannamoolkong and Sinpaldal#2 on the first and the second day of germination, respectively.

• Radiation Oncology Journal
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• v.8 no.1
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• pp.115-124
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• 1990

The characteristics of 23 MV photon beam have been presented with respect to clinical parameters of central axis depth dose, tissue-maxi mum ratios, scatter-maximum ratios, surface dose and scatter correction factors. The nominal accelerating potential was found to be $18.5\pm0.5$ MV on the central axis. The half-value layer (HVL) of this photon beam was measured with narrow beam geometry from central axis, and it has been showed the thickness of $24.5\;g/cm^2$. The tissue-maximum ratio values have been determined from measured percentage depth dose data. In our experimental dosimetry, the surface dose of maximum showed only $9.6\%$ of maximum dose at $10\times10\;cm^2$, 100 cm SSD, without blocking tray in. The TMR'S of $0\times0$ field size have been determined to get average $2.3\%$ uncertainties from three different methodis; are zero effective attenuation coefficient, non-ilnear least square fit of TMR's data and effective linear attenuation coefficient from the HVL of 23 MV photon beams of dual energy linear accelerator.