• Journal of Life Science
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• v.20 no.12
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• pp.1820-1828
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• 2010

Cobalt(II) chloride, a chemical compound with the formula$CoCl_2$, has been widely used in the treatment of anemia, as a chemical agent for the induction of hypoxia in cell cultures, and is known to activate hypoxic signaling. However, excessive exposure to cobalt is associated with several clinical conditions, including asthma, pneumonia, and hematological abnormalities, and can lead to tissue and cellular toxicity. It is also known to induce apoptosis. One of the questions was that of whether $CoCl_2$ might induce apoptosis via endoplasmic reticulum (ER) stress in neurons. To address this question, first, the level of DNA fragmentation was measured for assay of apoptotic rates using $CoCl_2$ with neuron PC12 cells. After confirmation of apoptosis inductions, under the same conditions, the expression levels of ER stress associated factors [ER chaperones Bip, calnexin, ERp72, ERp29, PDI, and ER membrane kinases (IRE1, ATF6, PERK)] were examined by RT-PCR and Western blotting. These results indicated that apoptosis is induced through activation of ER membrane kinases via ER stress. In conclusion, during induction of apoptosis through $CoCl_2$-induced hypoxia in neuron PC12 cells, ER membrane kinase of IRE1 was dominantly up-expressed, and, consecutively, TRAF2, which has been suggested to be one of the links connecting apoptosis and ER stress, was strongly up-expressed.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1368-1378
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• 2009

Unfolded protein response (UPR) is an important genomic response to endoplasmic reticulum (ER) stress. The ER response is characterized by changes in specific proteins, induction of ER chaperones and degradation of misfolded proteins. Also, the pathogenesis of several diseases like Alzheimer's disease, neuronal degenerative diseases, and diabetes reveal the role of ER stress as one of the causative mechanisms. Borneolum has been used for neuronal disease in oriental medicine. In the present study, the protective effect of borneolum on thapsigargin-induced apoptosis in rat C6 glial cells. Treatment with C6 glial cells with 5 uM thapsigargin caused the loss of cell viability, and morphological change, which was associated with the elevation of intracellular $Ca^{++}$ level, the increase in Grp78 and CHOP and cleavage of pro-caspase 12 Furthermore, thapsigargin induced Grp98, XBP1, and ATF4 protein expression in C6 glial cells. Borneolum reduced thapsigargin-induced apoptosis through ER pathways. In the ER pathway, borneolum attenuated thapsigargin-induced elevations in Grp78, CHOP, ATF4, and XBP1 as well as reductions in pro-caspase 12 levels. Also, our data showed that borneolum protected thapsigargin-induced cytotoxicity in astrocytes from rat (P3) brain. Taken together, our data suggest that borneolum is neuroprotective against thapsigargin-induced ER stress in C6 glial cells and astrocytes. Accordingly, borneolum may be therapeutically useful for the treatment of thapsigargin-induced apoptosis in central nervous system.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.3
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• pp.165-173
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• 2000

This Paper presents the position tracking control of a closed-loop cylinder system using electro-rheological(ER) valve actuators. After manufacturing three sets of cylindrical ER valves on the basis of Bingham model of ER fluid, a 3 dof(degree-of-freedom) closed-loop cylinder system having the heave, roll and pitch motions is constructed. The governing equations of motion are derived using Lagrange's equation and a control model is formulated by considering nonlinear characteristics of the system. Sliding mode controllers are then designed fer these ER valve actuators in order to achieve position tracking control. The effectiveness of trajectory tracking control performance of the proposed cylinder system is demonstrated through computer simulation and experimental implementation of the sliding mode controller.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.565-568
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• 1995

This paper presents the position control of a closed-loop cylinder system using ER(electro-rheological)valve actuators. Following the field-dependent pressure analysis of the ER valve actuators on the basis of Bingham model of ER fluids, a 3 d.o.f. close-loop sylinder system having the heave, roll and pitch motions is proposed. The governing equations of motion are derived using Lagrange's equation, and a control model is established by considering system uncertain parameters such as load conditions. A sliding mode controller which has inherent robustness to system uncertainties is adopted to achieve robust tracking control performance. Tracking control results for sinusoidal trajectory were presented in order to demonstrate the effectiveness of the proposed control system.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2005.05a
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• pp.125-131
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• 2005

The purpose of the present study is to examine the flow characteristics of ER fluids between 2 port and 3 port rectangular tube brass electrodes. ER fluid is made silicon oil mixed with $2-3wt\%$ starch having hydrous particles. Flow visualization of the ER fluids were obtained by CCD camera measuring those of the clusters using an image processing technique. This research found the flow $rate(Q_L)$ with 0 kV /mm, 0.5kV/mm and 1.0kV/mm for $Q_L\;=\;0,\;0$ and $5.73cm^3/s$. When the strength of the electric field increased, the cluster of ER fluids are clearly strong along the rectangular tube and the flow rate(Q) decreased.

• Macromolecular Research
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• v.17 no.9
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• pp.672-681
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• 2009

A series of inert and photo-stable Er(III)-encapsulated complexes based on ${\pi}$-extended dendritic anthracene ligands bearing G3-aryl-ether dendron ([G3-AnX]-$CO_2H$), which retain different ${\pi}$-bridging systems, such as single (X= S), double (X= D) and triple (X= T) bonds was designed and synthesized to establish the structure-property relationship. The near infrared emission intensities of Er(III)-encapsulated complexes were enhanced dramatically by increasing the ${\pi}$-conjugated extension of anthracene ligands. The time-resolved luminescence spectra show monoexponential decays with a lifetime of $2.0{\sim}2.4ms$ for $Er^{3+}$ ions in thin films, and calculated intrinsic quantum yields of $Er^{3+}$ ions are in the range of $0.025{\sim}0.03%$. As a result, all Er(III)-encapsulated dendrimer complexes exhibit the near IR emission with the following order: $Er^{3+}-[G3-AnD]_3$(terpy) > $Er^{3+}-[G3-AnS]_3$(terpy) ${\approx}$ $Er^{3+}-[G3-AnT]_3$(terpy), because $Er^{3+}-[G3-AnD]_3$(terpy) has a higher relatively spectral overlap J value and energy transfer efficiency. In addition, the lack of detectable phosphorescence and no significant spectral dependence of the ${\pi}$-extended anthracene moieties on the solvent polarity support energy transfer from their singlet state to the central $Er^{3+}$ ion taking place in $Er^{3+}-[G3-AnX]_3$(terpy).

• The Korean Journal of Rheology
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• v.10 no.3
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• pp.165-172
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• 1998

본논문에서는 전기장 부하에 따라 유동성질이 변화하는 ER유체의 빙햄특성을 실험 적으로 연구하였다. 특히 ER유체의 빙행특성에 영향을 주는 여러인자중 전극 간격 및 재질 에 따른 ER유체의 항복전단응력과 전류밀도의 변화를 온도에 따라 고찰하였다. 이를 위하 여 전극 간격을 가변시킬수 있는 전기 점도계를 세가지 재질로 자체 제작하였다. 전극간격 은 0.75 mm, 1.00mm 및 1.25 mm 로 설정하였으며 전극 재질은 스테인레스 스텔, 동 그리 고 기계구조용 탄소강(SMS45C)을 사용했다. 한편 실험에 사용된 ER유체는 자체 조성한 수 계 ER유체인 ERF-1과 외국의 우수하다고 알려진 비수계 ER유체인 ERF-2 두가지를 선택 하였다. 실험은 $25^{\circ}C$와 7$0^{\circ}C$ 및 10$0^{\circ}C$에서 수행하였으며 전기장은 0-4kV/mm 범위에서 온 도 및 ER유체의 종류에 따라 부하 가능한 전압까지 공급하였다. 전단변형률 50, 100, 150, 200, 400, 600, 800, 1000 및 1200 s-1에서 얻은 전단응력 실험결과로부터 최소오차선형법을 이용하여 전단변형률 영에서 동적 항복전단응력 값을 도출하였으며 그결과로부터 전극 간격 및 재질에 따른 ER효과의 변화를 고찰하였다. 또한 상온과 10$0^{\circ}C$에서 4kV/mm의 전기장을 부하하여 전기장에대한 ER유체의 응답특성을 실험을 수행했다.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.22 no.4
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• pp.365-371
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• 2012

This paper presents robust control performance of a direct current(DC) motor with brake system adopting a giant electrorheological(GER) fluid, whose distinguished feature is an extremely high value of yield stress. As a first step, Bingham characteristics of the GER fluid is experimentally investigated using the Couette type electroviscometer. A cylindrical type of ER brake is then devised based on the Bingham model, and its braking torque is evaluated. Structural analysis of ER break is performed using ANSYS. After formulating the governing equation of motion for the DC motor with ER brake system, a sliding mode control algorithm, which is very robust to external disturbances and parameter uncertainties, is synthesized and experimentally realized in order to achieve desired rotational speed trajectories. The tracking responses of the control system are then evaluated and verified by presenting speed control performance.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.3 no.3
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• pp.86-92
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• 2004

This paper describes the properties of temperature-viscosity characteristics of hydrous and anhydrous electro-rheological fluids containing starch and titanium particle in silicone oil ER effects arise from electrostatic forces between the starch particles and titanium particles dispersed to the electrically insulating silicone oil induced when electric field is applied ER fluids under electric field control have been found to provide resonable estimates of ER fluid viscosity variation characteristics. Yield shear stress of the ER fluids were measured the couette cell type rheometer as a function of electrlc fields. The outer cup is connected to positive electrode(+) and bob becomes ground(-). The electrie field is applied by high voltage DC power supply. In this experiment shear rates were increased from 0 to 200 $s^{-1}$ in 2 minutes. The ER fluid's viscosity change is very small and stable at the temperature range of $40^{\circ}C$ to $60^{\circ}C$. Therefore, applications of a new ER fluid to control systems application are suitable.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.1
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• pp.15-20
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• 2018

Most proteins produced in the endoplasmic reticulum (ER) of eukaryotic cells fold via disulfide formation (oxidative folding). Oxidative folding is catalyzed by protein disulfide isomerase (PDI) and PDI-related ER protein thiol disulfide oxidoreductases (ER oxidoreductases). In yeast and mammals, ER oxidoreductin-1s (ERO1s) supply oxidizing equivalent to the active centers of PDI. We previously identified and characterized the ERO1 of Bombyx mori (bERO1) as a thioredoxin-like protein that shares primary sequence homology with other ERO1s. Here we compare the reactivation of inactivated rRNase and sRNase by bERO1, and show that bERO1 and bPDI cooperatively refold denatured RNase A. This is the first result suggesting that bERO1 plays an essential role in ER quality control through the combined activities of bERO1 and bPDI as a catalyst of protein folding in the ER and sustaining cellular redox homeostasis.