• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.76-76
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• 2017

Buckwheat has gained scientists concern due to its nutritional and medicinal values in recent years, and many important bioactive compounds. The present study was performed to investigate the influence of the varietal performance and environmental conditions on the content of rutin and quercetin in Tartary buckwheat germplasm. A total of 44 foreign Tartary buckwheat germplasms were examined and compared their contents based on the collected countries, seed shape, and seed color. The highest number of germplasm (16) was found in ranged from 600 mg/100 g to 800 mg/100 g of rutin content. In case of quercetin content, the highest number of germplasm (19) was observed in ranged from 5 mg/100 g to 10 mg/100 g. However, the rutin content showed the highest value (1326.6 mg/100 g) from CBU408 and quercetin content showed the highest value (22.5 mg/100 g) from CBU456. The number of germplasm showed the highest value (23) in ranged from 3000 mg/100 g to 4000 mg/100 g of rutin content. The number of germplasm is the highest value (30) in ranged from 100 mg/100 g to 200 mg/100 g of quercetin content. When rutin and quercetin content in seed was compared according to collected countries, seed shape, and seed color respectively, no association was appeared with rutin and quercetin content. In sprout of whole Tartary buckwheat germplasm, mean of rutin content and quercetin content was 3362.9 mg/100 g and 14.2 mg/100 g respectively. In conclusion, the results observed from the present study suggest that highly rutin germplasm could be used more efficiently via breeding to develop a highly rutin content variety for using in sprout. No correlation was appeared in the case of quercetin. However, the quercetin content in sprout was 4~90 times more than seed. So, sprout could be used more efficiently than seed in the case of quercetin. Our results suggest that the varietal variation and qualitative characteristic differences of rutin and bioactive compounds may provide important nutrient sources and industrial application of Tartary buckwheat.

• 생약학회지
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• 제23권4호
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• pp.229-233
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• 1992

From the leaves of Ulmus parviflora isoquercitrin and rutin were isolated and identified by chemical and spectral analysis. The content of rutin was determined by HPLC, and it was about 2.81% in methanol extract.

• 생명과학회지
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• 제28권8호
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• pp.977-984
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• 2018

본 연구는 메밀의 발아 조건을 달리하여 메밀싹에서 생리활성 물질인 루틴의 함량을 높이고자 하였다. 가장 많이 재배되는 일반메밀과 타타리메밀을 이용하였고, 두 종자의 루틴 함량을 비교한 결과, 타타리메밀의 루틴 함량이 487 ppm으로 일반메밀과 비교하여 약 36배 높게 나타났다. 발아 후 싹으로 성장에 따른 루틴 함량을 분석한 결과, 일반메밀과 타타리메밀의 싹 모두 길이 생장에 따라서 루틴 함량도 증가하는 경향을 나타내었다 또한, 발아 및 싹의 성장 시에 빛을 조사한 일반메밀과 타타리메밀의 싹은 루틴 함량이 빛을 조사하지 않은 경우에 비해 상대적으로 높았다. 타타리메밀의 경우 10 cm 이상의 싹은 4,579 ppm(빛을 조사하지 않은 경우), 5,160 ppm(빛을 조사한 경우)로 나타났다. 메밀싹의 성장 시에 빛이 루틴의 합성에 영향을 끼지는 것으로 판단되었다. 이에 LED 빛의 세기를 2,000 Lux에서 22,000 Lux까지 점차 증가시키면서 타타리메밀싹을 제조하였고, 가장 높은 세기인 22,000 Lux에서 가장 높은 루틴 함량을 가졌다. 타타리메밀싹의 열수 추출물은 85.47 ppm, 에탄올 추출물의 경우에는 1,225.85 ppm의 루틴 함량을 나타내었다. 14,000 Lux까지는 급격하게 루틴 함량이 증가하였으나, 그 후에는 증가폭이 줄어들어 메밀싹은 14,000 Lux에서 10 cm 이상 성장한 것이 최적의 루틴을 포함하는 메밀싹 제조에 가장 적합하다고 판단되었다. 열처리($70{\sim}90^{\circ}C$의 온도 범위)를 한 추출물의 루틴 함량이 미처리군과 비교하여 유의적인 차이를 나타내지 않았다. 이 결과 메밀에서 추출한 루틴은 열안정성을 가진다고 판단되었다.

• 한국식품과학회지
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• 제26권6호
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• pp.759-763
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• 1994

약성(藥性)을 갖고 여러가지 질병에 유익한 메밀을 채소로 식용하고자 무기성분과 rutin의 함량을 개량종 9품종에서 각각 측정하였다. 9품종의 종실에 대한 일반 성분은 조단백 함량이 $16.2{\sim}20.4%$, 조지방은 $2.2{\sim}2.9%$이었다. 회분 함량은 $2.4{\sim}43%$로 재래종 $(2.4{\sim}2.6%)$에 비해 우수하였으며, 섬유소는 재래종에 비해 $2{\sim}3%$ 낮으며 전체적으로 당질의 함량이 다소 낮았다. 개량중 중 수원 11호와 5호가 각각 0.12%, 0.11%로 높은 rutin 함량을 보였다. 무기물은 회분에 대하여 10% HCI 불용물이 $0.5{\sim}55.32%$ 였으며 K, Ca, Mg 및 Fe 순으로 함유되어 재래종에 비해 우수하였다. 메밀채소의 일반성분은 높은 단백질 함량을 보였으며 수원 10, 11호가 가장 높은 단백질 함량을 보였다. 메밀 채소증 rutin 함량은 $0.20{\sim}0.40%$로 수원 5호가 가장높은 0.40% 함량을 보였다. 메밀함량이 높은 수원 5호의 성장과정중의 rutin함량의 변화는 떡잎시 0.40%, 본잎 $4{\sim}5$장시 1.10%였다. 또한 무기성분의 변화는 10% HCI 불용물이 성장초인 떡잎시 69.8%인 반면 완전 성장시기인 개화시 4.68%였다. Fe와 Ca는 채소성장과 더불어 증가되었다.

• 대한약침학회지
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• 제20권1호
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• pp.52-56
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• 2017

Objectives: Neutrophils represent the front line of human defense against infections. Immediately after stimulation, neutrophilic enzymes are activated and produce toxic mediators such as pro-inflammatory cytokines, nitric oxide (NO) and myeloperoxidase (MPO). These mediators can be toxic not only to infectious agents but also to host tissues. Because flavonoids exhibit antioxidant and anti-inflammatory effects, they are subjects of interest for pharmacological modulation of inflammation. In the present study, the effects of rutin on stimulus-induced NO and tumor necrosis factor $(TNF)-{\alpha}$ productions and MPO activity in human neutrophils were investigated. Methods: Human peripheral blood neutrophils were isolated using Ficoll-Hypaque density gradient centrifugation coupled with dextran T500 sedimentation. The cell preparations containing > 98% granulocytes were determined by morphological examination through Giemsa staining. Neutrophils were cultured in complete Roswell Park Memorial Institute (RPMI) medium, pre-incubated with or without rutin ($25{\mu}M$) for 45 minutes, and stimulated with phorbol 12-myristate 13-acetate (PMA). Then, the $TNF-{\alpha}$, NO and MPO productions were analyzed using enzyme-linked immunosorbent assay (ELISA), Griess Reagent, and MPO assay kits, respectively. Also, the viability of human neutrophils was assessed using tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and neutrophils were treated with various concentrations of rutin ($1-100{\mu}M$), after which MTT was appended and incubated at $37^{\circ}C$ for 4 hour. Results: Rutin at concentrations up to $100{\mu}M$ did not affect neutrophil viability during the 4-hour incubation period. Rutin significantly decreased the NO and $TNF-{\alpha}$ productions in human peripheral blood neutrophils compared to PMA-control cells (P < 0.001). Also, MPO activity was significantly reduced by rutin (P < 0.001). Conclusion: In this in vitro study, rutin had an anti-inflammatory effect due to its inhibiting NO and $TNF-{\alpha}$ productions, as well as MPO activity, in activated human neutrophils. Treatment with rutin may be considered as a therapeutic strategy for neutrophil-mediated inflammatory/autoimmune diseases.

• 폴리머
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• 제36권4호
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• pp.420-426
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• 2012

본 연구에서는 항산화 물질로 잘 알려진 quercetin과 그 배당체인 rutin을 함유하는 poly(${\varepsilon}$-caprolactone)-b-poly(ethylene glycol) 미셀을 제조하여, 활성물질(quercetin, rutin)의 in vitro 피부 흡수 증진에 관한 연구를 수행하였다. 입자크기는 PCL-b-PEG 고분자의 농도가 증가함에 따라 미셀의 초기 입자 크기가 증가하는 경향을 보였다. 고분자 미셀의 표면 전위(Zeta potential)는 비교적 일정함을 확인하였다. 제조한 고분자 미셀의 피부 흡수력을 평가하기 위하여, 용액 상태의 활성물질을 미셀의 대조군으로 하여 Franz cell을 이용한 투과실험을 진행한 결과 용액 상태보다 미셀에서 더 높게 나타났음을 확인하였다. 또한 화장품 소재로서의 안전성 평가를 위한 인체 피부 일차자극 실험(patch test) 결과 어떠한 피부 자극도 관찰되지 않았다.

• 농업과학연구
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• 제38권1호
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• pp.87-94
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• 2011

Flavonoid contents of common buckwheat (cv. Kitawase) and tartary buckwheat (cv. Hokkai T 8, Hokkai T 9 and Hokkai T 10) were determined by high-performance liquid chromatography (HPLC). Moreover, they were measured at different plant developments such as 10, 18, 20, 22 and 30 days after sowing (DAS) and with plant parts including leaf, stem and flower harvested at 30 DAS. Total flavonoids including chlorogenic acid, four kinds of C-glycosylflavones (orientin, isoorientin, vitexin, isovitexin) and rutin of tartary buckwheats (range of 44.2-54.7, mean 44.2) were found 35% higher than those of common buckwheat (28.9 mg/g dry wt.). Among them, rutin was measured above 80% of total flavonoid contents. The other flavonoids (chlorogenic acid and four kinds of C-glycosylflavones) presented the highest level at 10 DAS and decreased according to plant developments. On the other hand, rutin content of Kitawase presented the highest level (33.6 mg/g dry wt.) at 22 DAS and decreased up to 30 DAS. Rutin content in tartary buckwheat temporarily decreased from 10 to 18 DAS and then reversely increased up to 30 DAS presented the highest level as 'U' curve. In Hokkai T 10, rutin content was found the highest level (53.8 mg/g dry wt.) at 30 DAS. In different plant parts harvested at 30 DAS, rutin content of leaf (range of 42.8-68.0, mean 57.0) was 5.3-fold higher than that of stem (range of 8.0-15.9, 10.8 mg/g dry wt.), regardless of cultivar. Significantly, rutin content (78.7) in the flower of Kitawase was 1.8 times higher than in the leaf and 9.8 times in the stem. Especially, chlorogenic acid content (14.6 mg/g dry wt.) in the flower of Kitawase was 63-fold higher than in the leaf, and 20-fold in the stem.

• Natural Product Sciences
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• 제23권3호
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• pp.222-226
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• 2017

Flavonoids including quercetin and rutin are a group of naturally occurring compounds widely distributed in plants, especially in buckwheat. Thus, cereal and the leaf of the plant have increasingly used as a source of nutritional and functional foods such as noodle, cake or soup in Korea, Japan and other countries. This study investigated comparative effects of dietary rutin rich in buckwheat and its aglycone, quercetin, on serum biomarkers and antioxidant parameters in rats treated with chronic ethanol. Rats were fed with the liquid diets prepared by the method of Lieber Decarli. Serum alanine transaminase (ALT) and aspartate transaminase (AST) activities increased significantly by alcohol feeding. Dietary flavonoids including rutin, quercetin and their mixtures (1/1, v/v) decreased significantly the activities of serum ALT whereas the feeding of quercetin decreased only the activity of serum AST. The concentration of serum malondialdehydes elevated by chronic alcohol feeding decreased markedly in all the experimental groups that were fed with the flavonoids; however, the combined administration of quercetin or rutin, but not that of rutin or quercetin alone decreased significantly the concentration of liver malondialdehydes to the normal range in rats fed without ethanol. Our results suggested that dietary combined mixture of rutin and quercetin might be effective in ameliorating adverse responses seen in rats exposed to ethanol chronically.

• 한국작물학회지
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• 제53권3호
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• pp.279-284
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• 2008

녹두의 식물체 부위, 수확시기, 생육단계, 파종기 등에 따른 주요 flavonoids의 함량 차이를 구명하고자 본 연구를 수행하였다. 1. Vitexin과 isovitexin은 녹두 식물체 부위 중에서 종실에만 분포하고 잎, 엽병, 줄기, 뿌리에서는 검출되지 않았다. 2. 녹두 종피에만 vitexin은 $52.1\;mg{\cdot}g^{-1}$, isovitexin은 $51.7\;mg{\cdot}g^{-1}$ 분포하고 자엽에서는 검출되지 않았다. 3. 녹두 조 적 만기 수확에 따른 vitexin과 isovitexin 함량은 차이가 없었다. 4. 녹두 잎에 다량 함유된 기능성 물질을 분리 동정한 결과 rutin이었다. 5. Rutin함량은 녹두 잎, 엽병, 줄기 순으로 많고, 종실, 뿌리, 협에서는 검출되지 않았다. 6. 녹두 잎, 엽병, 줄기에 함유된 rutin은 3, 5, 7엽기 순으로 유묘기일수록 많았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.52-52
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• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.