• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.323-330
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• 2009

Nontuberculous mycobacteria (NTM) are a major cause of opportunistic infections in immunocompromised patients, making the reliable and rapid identification of NTM to the species level very important for the treatment of such patients. Therefore, this study evaluated the usefulness of the novel target genes tuf and tmRNA for the identification of NTM to the species level, using a PCRrestriction fragment length polymorphism analysis (PRA). A total of 44 reference strains and 17 clinical isolates of the genus Mycobacterium were used. The 741 bp or 744 bp tuf genes were amplified, restricted with two restriction enzymes (HaeIII/MboI), and sequenced. The tuf gene-PRA patterns were compared with those for the tmRNA (AvaII), hsp65 (HaeIII/HphI), rpoB (MspI/HaeIII), and 16S rRNA (HaeIII) genes. For the reference strains, the tuf gene-PRA yielded 43 HaeIII patterns, of which 35 (81.4%) showed unique patterns on the species level, whereas the tmRNA, hsp65, rpoB, and 16S rRNA-PRAs only showed 10 (23.3%), 32 (74.4%), 19 (44.2%), and 3 (7%) unique patterns after single digestion, respectively. The tuf gene-PRA produced a clear distinction between closely related NTM species, such as M. abscessus (557-84-58) and M. chelonae (477-84-80-58), and M. kansasii (141-136-80-63-58-54-51) and M. gastri (141-136-117-80-58-51). No difference was observed between the tuf-PRA patterns for the reference strains and clinical isolates. Thus, a diagnostic algorithm using a tuf gene-targeting PRA is a promising tool with more advantages than the previously used hsp65, rpoB, and 16S rRNA genes for the identification of NTM to the species level.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.563-563
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• 2005

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.295.1-295
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• 1998

No Abstract, See Full Text

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.56.1-56.1
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• 2006

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.226.1-226
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• 1999

No Abstract, See Full Text

• Journal of Microbiology
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• v.43 no.2
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• pp.213-218
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• 2005

Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampinresistant genotype, Asn$(AAC)^$442, according to the results of 16S rRNA and rpoB gene analyses

• Journal of radiological science and technology
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• v.40 no.4
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• pp.543-548
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• 2017

The aim of this study was to evaluate anteroposterior oblique(RPO, LPO) and posteroanterior oblique(LAO, RAO) projections of the cervical spine, at various kVp and mA s increments, in order to compare thyroid surface dose. Using Rando phantom, dosimeter was attached to the Cervical spine 4~5 to measure the surface dose in the same thyroid position. As a result, the surface dose was $595.08{\pm}215.01{\mu}Gy$ for anteroposterior oblique(RPO, LPO) projections and $64.21{\pm}33.49{\mu}Gy$ for posteroanterior oblique(LAO, RAO) projections by changing kVp increment. The surface dose was $445.20{\pm}230.90{\mu}Gy$ for anteroposterior oblique(RPO, LPO) projections and $44.51{\pm}22.77{\mu}Gy$ for posteroanterior oblique(LAO, RAO) projections by changing mAs increment. The posteroanterior oblique method could reduce about 90% the surface dose than the anteroposterior oblique method. There were statistically significant differences among the examinations(p<0.001). Change the direction of position to reduce the surface dose at oblique projection of cervical spine. Therefore, we consider posteroanterior oblique projections than anteroposterior oblique projections of cervical spine examination in other to reduce patient surface dose.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.553-560
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• 2005

The stf (Salmonella typhimurium fimbriae) operon consisting of stfA(CDEFG assumes to encode putative fimbriae. The complete stf operon is existed in S. typhimurium and S. choleraesuis, whereas it is absent in S. typhi. Analyses of the amino acid residues between major subunit StfA of the Stf fimbriae and those of known other fimbriaes suggested that Stf belongs to class I type fimbriae. Through comparison of StfD chaperone with the other fimbrial chaperones, and of C-terminus in subunits of Stf fimbriae, it belongs to FGS (with a short Fl-G1 loop) subfamily. In order to investigate the expression of stf operon, we have constructed a Salmonella strain containing a chromosomal stfA::lacZYA transcriptional fusion, resulting in S. typhimurium $_X8532$. The strain $_X8532$ lacked the expression of \beta-galactosidase$ under normal culture conditions. However, with longer incubation time of the S. typhimurium $_X8532$, we have isolated 21 individual strains exhibiting $Lac^+$ phenotype. $Lac^+$ phenotype was appeared as approximately 0.03 frequency per generation. All isolates expressed lacZ constitutively in the various environmental conditions. Various global regulatory proteins including RpoS, OmpR, and CpxR were not involved in the regulation of the stf operon. A S. typhimurium $_X8661$ mutant lacking stfAC function attenuated 6.7 folds more than that of wild type $_X3761$ in the mouse virulence test, suggesting in the somehow involved in the Salmonella pathogenesis.

• Journal of KIISE:Information Networking
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• v.27 no.3
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• pp.315-322
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• 2000

네트워크에서 QoS를 보장하기 위해 최근에 제안되는 패킷 스케줄링 알고리즘은 대부분 우선 순위에 입각한 패킷 전송 서비스를 한다. 이러한 우선 순위를 유지하기 위한 큐의 관리에는 많은 비용이 들므로 QoS를 보장하는 네트워크에서 우선 순위 큐의 관리 비용을 줄이는 노력이 필요하다. 패킷 스케줄링 알고리즘 중 RPO+(Rotate Priority Queue)는 우선 순위 FIFO(First in first out)큐를 사용하여 주기 적으로 재명명되는 패킷 스케줄링 알고리즘이다. FIFO 큐에 패킷들을 근사 정렬하여 패킷의 우선 순위를 유지하므로 계산 복잡도를 줄이지만, 패킷 우선 순위를 유지하기 위해 2배(2P)의 큐를 필요로 한다.[1] 본 논문에서는 필요한 큐의 개수를 P개의 큐로 제한하여 큐에 대한 관리 비용을 줄였으며 엔터프라이즈 환경에서 애플리케이션이 요구하는 서비스 특성에 따라 클래스로 구분하여 적합한 패킷 스케줄링 서비스를 제공하는 알고리즘을 제시한다. 본 기법은 추가적인 오버플로우 큐를 관리하고 패킷 어드미션 컨트롤러를 통해 패킷 전송 지연 시간을 제한함으로 다양한 애플리케이션의 네트워크 QoS 요구를 보장하고 패킷 전손 효율을 높였다.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.