• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.2
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• pp.113-122
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• 2007

Purpose: Platelet-rich plasma (PRP) is known to accelerate and/or enhance hard and soft tissue healing and regeneration. As such, PRP has been used in various clinical fields of surgery. Recently there have been several attempts to use PRP in the field of tissue engineering. However, some controversies still exist on exact mechanism and benefits of PRP. Therefore various animal experiments are necessary to reveal the effect of the PRP. However, even if animal experiment is performed, the efficacy of the experiment could not be validated due to absence of an animal PRP model. The purpose of this study is to establish rat PRP model by comparing several PRP fabricating methods, and to assay growth factor concentration in the PRP. Materials and methods: Rat blood samples were collected from nine SD rat (body weight: 600-800g). PRP was prepared using three different PRP fabricating methods according to previously reported literatures. (Method 1: 800 rpm, 15 minute, single centrifuge; Method 2: 1000 rpm, 10 minute, double centrifuge; Method 3: 3000 rpm, 4min and 2500 rpm, 8 min, double centrifuge). Platelet counts were evaluated in an automated machine before and after PRP fabrications. In terms of growth factor assay, prepared PRP were activated with 100 unit thrombin and 10% calcium chloride. Growth factor (PDGF-BB, VEGF) concentrations on incubation time were determined by sandwich-ELISA technique. Results: An average of 3ml (via infraorbital venous plexus) to 15ml (via celiac axis) the rat blood could be collected. By using Method 3 (3000 rpm, 4 min and 2500 rpm, 8 min, double centrifugation), around 1.5ml of PRP could be prepared. This method allowed us to concentrate platelet 3.77-fold on average. PDGF-BB concentration (mean, 1942.10 pg/ml after 1 hour incubation) and VEGF concentration (mean, 952.71 pg/ml after 1 hour incubation) in activated PRP were higher than those in untreated blood. Also PDGF-BB showed constant concentration during 4-hour incubation, while VEGF concentration was decreased after 1 hour. Conclusion: Total 11,000 g minute separation and condensation double centrifuge method can produce efficient platelet-rich plasma. Platelet-rich plasma activated with thrombin has showed higher concentrations of growth factors such as PDGF-BB and VEGF, compared to the control group. Platelet-rich plasma model in a rat model was confirmed in this study.

• Journal of the Korean Society of Marine Environment & Safety
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• v.18 no.3
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• pp.273-278
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• 2012

The use of Al alloys instead of fiber-reinforced plastic(FRP) in ship construction has increased because of the advantages of Al-alloy ships, including high speed, increased load capacity, and ease of recycling. This paper describes the effects of probe diameter on the optimum friction stir welding conditions of 5456-H116 alloy for leisure ship, measured by a tensile test. In friction stir welding using a probe diameter of 5 mm under various travel and rotation speed conditions, the best performance was achieved with a travel speed of 61 mm/min. Using a probe diameter of 6 mm, rotation speeds of 170-210 rpm, and a travel speed of 15 mm/min produced a rough surface and voids because of insufficient heat input produced by the low rotation speed. At 500-800 rpm, chips were observed, although there were no voids, and the weld surface was excellent. However, at 1100-2500 rpm, many chips were produced due to excessive heat input. Heat effects were very evident on the bottom. For a travel speed of 15 mm/min, heat input caused by friction increased as the rotation speed increased. The mechanical characteristics were degraded by accelerated softening due to increasing heat input.

• Resources Recycling
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• v.27 no.1
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• pp.45-54
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• 2018

This study aimed to investigate the effects of mechanical factors such as agitation speed and air rate in fly ash flotation. Specifically, we used thermal power plant fly ash with unburned carbon content of 3.4 to 3.7%. The effect of pH, agitation speed, collector dosage, and frother dosage - the key factors of froth flotation - showed unburned carbon recovery and unburned carbon content of 63% and 34%, respectively, when the dosage of safflower oil used as collector was 800 g/ton, pH was 7, agitation speed was 1,200 rpm, and frother dosage was 400 g/ton. The SEM/EDS analysis of fly ash in that case indicated that the spherical fly ash particles lowered the unburned carbon content as they floated with the air bubbles without being dissolved in the unburned carbon or settled in the ore solution. The other experiment of changing the mechanical factors such as agitation speed and air rate resulted in unburned carbon recovery and unburned carbon content of 74% and 67%, respectively, at air rate of 8 L/min and agitation speed of 900 rpm. The recovery and unburned carbon content increased as the low agitation speed and additional air injection decreased the strength of the eddy current in the ore solution and consequently prevented the floating of fine fly ash particles with unburned carbon. In addition, the recovery rate and unburned carbon increased further to 80% and 70%, respectively, showing the best performance when the agitation speed and air rate were lowered to 800 rpm and 6 L/min, respectively.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.769-774
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• 1993

Defatted rice bran was microparticulated using fluidized bed opposed jet mill and air-classified at different air classifying wheel speed (ACWS) in Turboplex classifier. The median particle size and the standard deviation decreased, and concomitantly the specific surface area increased generally with increasing ACWS. The protein, fat and ash contents of the recovered rice bran increased with ACWS. The contents of minerals; magnesium, zinc, iron and manganese; increased positively with ACWS. The phytic acid content, however, was slightly higher at middle ACWS. The dietary fiber content was highest in the ACWS 15,000 rpm fraction showing 31.47%. Higher ACWS resulted in lighter colored powder. The water holding capacity (WHC) showed the maximum value at ACWS 12,000 rpm and decreased with increasing ACWS, while the oil holding capacity (OHC) increased with ACWS. The rheological property of the microparticulated rice bran/water suspension fitted to the linear model. The yield stress and viscosity of the suspension increased with ACWS. The shape of microparticulated rice bran at ACWS 21,000 rpm was spherical, and the median particle size was $3.7{\mu}m$. When cake was prepared with substitution of microparticulated rice bran at 5%, the cake height and volume increased remarkably.

• Journal of Food Hygiene and Safety
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• v.15 no.2
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• pp.114-121
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• 2000

A study was carried out to establish a detection method for irradiated black and white pepper. Samples were packed in polyethylene bags and irradiated with 2.5, 5, 7.5, 10 and 15 kGy using a Co-60 irradiator. The samples were suspended in water, and alkalized with sodium hydroxide solution. Apparent viscosity was determined after heat gelatinization using a Brookfield DV-III rotation viscometer at 30。C with 30, 60, 90, 120, 150, 180, and 210 rpm. Means and standard deviations of the viscosities of all samples decreased by increasing the stirring speeds. The viscosities increased in all samples by increasing the concentration. Regression expressions and coefficients of viscosity which decreased with increasing irradiation dose of 10% and 13% black pepper, and 7% and 10% white pepper were 0.9531 (y=-131.29x+1,769.0), 0.9725 (y=-351.33x+4,036.0), 0.9731 (y=2,208.0e${^-0.3546X}$), and 0.9959 (y=5,116.0e${^-0.2887X}$), respectively, at 120 rpm. This trend was similar fur all stirring speeds. These results suggest that the detection of irradiated black and white pepper at various doses is possible by the viscometric method.

• Journal of Korea Water Resources Association
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• v.48 no.11
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• pp.937-944
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• 2015

This study proposed a novel technique, namely the Radar Polygon Method (RPM), for areal rainfall estimation based on radar precipitation data. The RPM algorithm has the following steps: 1. Determine a map of the similar rainfall occurrence of which each grid cell contains the binary information on whether the grid cell rainfall is similar to that of the observation gage; 2. Determine the similar rainfall probability map for each gage of which each grid cell contains the probability of having the rainfall similar to that of the observation gage; 3. Determine the governing territory of each gage by comparing the probability maps of the gages. RPM method was applied to the Anseong stream basin. Radar Polygons and Thiessen Polygons of the study area were similar to each other with the difference between the two being greater for the rain gage highly influenced by the orography. However, the weight factor between the two were similar with each other. The significance of this study is to pioneer a new application field of radar rainfall data that has been limited due to short observation period and low accuracy.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.5
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• pp.307-313
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• 2011

As production of LCD increases, it has become necessary to find an economically efficient way of treating LCD wastewater with high concentration of fluoride. This study focuses on the calcium sources : $CaCl_2$, $Ca(OH)_2$ and $CaCO_3$ for the treatment of the LCD wastewater including high concentration of fluoride. Of course considering removal efficiency and economical aspect, study is continued. Then this study have objective giving aid to field. Consequently, each calcium source's removal efficiency was measured in various pH, calcium dosage, reaction time, and mixing intensity. The optimum operational conditions for $CaCl_2$ were found to be pH of 7, calcium dosage of 0.4[Ca]/[F] (mol / mol), 1 hr of operation and 200 rpm of mixing intensity. For $Ca(OH)_2$, they were pH of 7, calcium dosage of 30 mL/L, 1 hr of operation, and 200 rpm of mixing intensity. While $CaCO_3$ had operational conditions of pH of 4, calcium dosage of 30 mL/L, 1 hr operation and 200 rpm of mixing intensity. But it is recommended to use calcium sources according to various field conditions.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.14 no.2
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• pp.113-122
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• 2016

In this study, the dispersion process of pollutant substances in a port under wave and current environments was evaluated by a hydraulic experiment. Once the contaminants washed ashore into the port of Wolseong nuclear power plant, transport processes of pollutants were investigated by tracking the tracer according to the variations of experimental condition through a hydraulic experiment. Several hydraulic experiments were performed to analyze the pollutant discharge rate of the surface coming from the different coastal environments. From the hydraulic experiment results, the tracer concentration decreased exponentially. These results suggested that, after the tracer was transported to the open sea, a different gradient was shown under different conditions. For the case of a diluted condition, the half-life of flow rate was 2.70, 10.40, and 26.39 days for 30, 20 and 10 rpm in the left-side, respectively. The decrease of the tracer concentration under conditions of 30 rpm was much faster than that under conditions of 10 rpm. For the wave condition, the half-life of flow rate was 4.59 and 15.35 days for the right and left side of the port in a hydraulic scale prototype, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.8
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• pp.1045-1050
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• 2006

Optimum operation condition for pilot scale of alkaline processing for fish muscle was investigated by measuring protein solubility, yield, texture, and water-holding capacity. Recovered protein yield was 33.2% for whole fish and 61.8% for minced muscle. Optimum homogenized speed and time, using industrial scale homogenizer, were 3,000 rpm and 5 min, respectively. Limited centrifugal force of continuous cylinder type was 4,000 rpm for recovering soluble protein, and 2,000 rpm for recovering precipitated proteins. The pH control agents such as citric acid, sodium phosphate and calcium oxide decreased the breaking force and deformation of recovered protein gel. The breaking force and deformation of the recovered proteins were high compared to conventional surimi. The breaking force and deformation were decreased by addition of salt, starch and bovine plasma proteins. Whiteness of recovered protein gel was lower than that of conventional surimi. Alkaline processing greatly decreased nitrogen content and chemical oxygen demand in waste water. The results suggest that alkaline processing has a potential as industrial production for recovering the proteins from fish muscle.

• Journal of the Microelectronics and Packaging Society
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• v.16 no.1
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• pp.45-50
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• 2009

By Laser Through-Silicon-Via process, debris and particles occur when you are forming. Therefore the research of TSV cleaning become important to remove those particles and debris. Both chemical cleaning method that uses a surfactant and physical cleaning method that uses a brush were studied with the via of $30{\mu}m$ diameter and $100{\mu}m$ depth on the 8 inch CMOS Image Sensor wafer. On the DI water and a surfactant in mixture ratio of 2:1, debris show $73{\mu}m^2$ per $0.054mm^2$. Cleaning is superior by lower mixture ratio of DI water and surfactant. In addition, It is less than 5% of debris distribution in the laser condition changed by Laser's frequency and its speed and cleaning had no effect. In the physical cleaning, there are no crack and damage when the system condition is set by $1000{\sim}3000rpm$ strip, $50{\sim}3000rpm$ rinsing, and $200{\sim}300rpm$ brushing Therefore, debris and particles can be removed by enforced chemical method and physical method.