• Journal of Ecology and Environment
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• v.36 no.3
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• pp.151-158
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• 2013

We estimated phenotypic selection on the height and number of flowering-stalks in a rosette annual Cardamine hirsuta by applying path analysis to the data collected at three natural populations located in central Japan. The path from rosette size was positively connected with the fruit production through the both height and number of flowering-stalks. In the all three populations, the paths from the number of stalks were more strongly connected with the fruit production than from the height of stalks. The paths from the rosette size showed similar magnitude with the number of stalks and the height of stalks. The direct path from rosette size to the fruit production was detected only at one site. These results suggest stronger phenotypic selection on the rosette size through the number of stalks than the height of stalks. The lateral branching rather than increment of individual inflorescence size is the major response to control the fruit production for C. hirsuta growing in a natural habitat.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.493-496
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• 2016

Peach rosette mosaic virus (PRMV) is a plant virus that was first reported in 1933 by Peach. It can infect hosts including peach, grape, blueberry, dandelion, plum, cherry tree, and weeds. PRMV is non-reportable in Korea, but it is designated as a controlled virus requiring plant quarantine. In this study, for the rapid and specific detection of PRMV, we developed an assay using loop-mediated isothermal amplification (LAMP). Comparison between conventional polymerase chain reaction (PCR) methods (real time-PCR and nested PCR) and LAMP for the detection of PRMV revealed an equivalent level of sensitivity by all the tested methods. For the LAMP assay, outer primer sets were used to amplify a 264-bp PCR product, which was then digested using the restriction enzyme Pvu II (CAG/CTG), and the visualization of two digestion fragments (207 + 57 bp) indicated a positive reaction. The developed LAMP assay for PRMV is expected to enable the rapid monitoring of PRMV in plants.

• Korean Journal of Environmental Agriculture
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• v.22 no.4
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• pp.294-299
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• 2003

The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy) of gamma rays to investigate their germination rate and to quantity the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120Gy was not greatly affected but was rapidly decreased at over 160Gy. It seemed that lethal dose ($LD_{50}$) of germination was 160Gy. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40Gy. Multi-shoots were developed over 80Gy. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.9-13
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• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• The Plant Pathology Journal
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• v.17 no.1
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• pp.57-61
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• 2001

Phytoplasmas were identified from two chrysanthemum (Dendranthema grandiflorum) plants showing different symptoms ; one with stusting, rosette, and excessive branching (Ph-ch1), and the other with stunting and chlorosis (Ph-ch2). Electron microscopy of midrib of the plants with the symptoms revealed that numerous phytoplasmas were localized in the phloem cells. The disease was transmitted from infected plants to healthy ones by grafting. Phytoplasma-specific DNA was detected in polymerase chain reaction (PCR) analysis with template DNA extracted from the leaves of Ph-ch1 and Ph-ch2, both of which yielded a same DNA band corresponding to 1.5 kb. Using a specific primer pair (R16F1/R1) synthesized based on aster yellows (AY) phytoplasma, a DNA fragment of 1.1 kb was amplified by PCR. Endonuclease restriction patterns of the 1.1 kb PCR products from Ph-ch1 and Ph-ch2, which were dgeste with each of the restriction endonucleases Sau3A, Hha, Alu and Rsa, were same as those of AY phytoplasma from periwinkle. This suggests that the chrysanthemum plants (Ph-ch1 and Ph-ch2) be infected with a phytoplasma belonging to AY phytoplasma.

• The Korean Journal of Ecology
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• v.13 no.4
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• pp.331-342
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• 1990

Analysis of vegetation on the pavements and under the trees of streets in Seoul city were investigated from July to November 1989. All of the vascular herbs were 9 orders, 13 families, 30 genera, 37 species and 1 forma. Species diversity and evenness index were higher on the pavements than under the trees of streets. Eragrostis multicaulis with C-4 pathway was a dominant species, and next orders of importace values were Eleusine indica(C-4), Digitaria sanguinalis(C-4), Taraxacum officinale(C-3) and Erigeron annus(C-3). On the pavements and under the trees of streets, light intensity and surface temperature were very high, Also, these sites were affected by many kinds of human interferences. Therefore, it was an example of adaptation to specific ecological environments that C-4 plats were more dominant than C-3, growth forms of C-3 plants were rosette or prostrate, and annual or biennial plants were distributed in these studied sites. Generally, fruits types were grains, achens and capsules. Seeds were very small and some had appendages such as pappus.

• Journal of Ecology and Environment
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• v.42 no.3
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• pp.103-110
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• 2018

Backgrounds: In arid grasslands, wells are subject to heavy trampling and grazing pressure, which can increase vulnerability to local land degradation. To investigate trampling and grazing, we surveyed plant communities at three well sites in the desert steppe of Mongolia, using 1600-m line transects from the wells. The sites (Bshrub, Sshrub, and shrubL) differed by concomitant shrub type (big shrub, small shrub, and shrub-limited) and livestock pressure (light, medium, and heavy). A plant classification scheme based on edibility and morphology (rosette or creeping type) was used to separate grazing and trampling effects on plant communities. Results: Edible plants were dominant at all sites but a fraction of grazing- and trampling-tolerant plants increased in the order Bshrub, Sshrub, and shrubL, following livestock pressure. Clear transition zones from inedible to edible plant groups were recognized but at different locations and ranges among the sites. Trampling-tolerant plants explained 90% of inedible plants at Sshrub with camels and horses, but grazing-tolerant plants prevailed (60%) at shrubL with the largest livestock number. Plant coverage increased significantly along the transects at Bshrub and Sshrub but showed no meaningful change at shrubL. Herbaceous plant biomass showed significant positive and negative trends at Bshrub and shrubL, respectively. Conclusions: Both grazing and trampling can produce larger fractions of inedible plants; in this, camel and horses can have considerable effects on desert-steppe plant communities through trampling.

• Animal cells and systems
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• v.2 no.2
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• pp.239-242
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• 1998

Arabidopsis trp1 mutant plants, deficient in phosphoribosyI anthranilate transferase (PAT) activity, accumulate anthranilate compounds, which render them blue fluorescence. The visible phenotype of trp1 makes the PAT gene an excellent reporter gene in the mutant. In order to develop a system for the homologous recombination using the phenotypic characteristic of trp1-100, we established optimum conditions for the isolation and regenera tion of protoplast from auxin-conditioned, trp1-100 root cultures. Trvptophan had to be supplemented in the germination medium for the efficient cell division and subsequent plant regeneration. When 10 uM tryptophan was added to the germination medium, we obtained the highest yield of protoplasts ($3{\times}10^6 cells/g$) and the best viability (92%). Thirty percent of root protoplast derived from meristematic cells underwent cell division within 5 days in callus-induction medium. Regenerated rosette leaves (2-3 mm) were transferred to rooting medium and finally acclimated to the soil for flowering.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.185-189
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• 2009

This study was carried out to investigate the effect of shading (50% or not) and pruning height (3cm, 6cm, and 9cm) of remained above-ground plant part on shoot emergence aspect, mortality rate, flowering and cut flower characteristics of two years old Gypsophila paniculata plants. Days to shoot emergence (80%) advanced, emerged shoot number increased and mortality rate surveyed on late October decreased in 50% shading treatment. In addition to, mortality rate decreased, as above-ground plant part was highly pruned. Blooming was earlier with highly pruning the remained above-ground pan and under non-shading condition than shading. Growth traits such flower stalk length, primary branches number, stem diameter, and cut flower yield were the best and also non-particulate rate and rosette formation rate were the lowest in pruning height of 9cm under non-shading condition.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.194.2-195
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• 2003

Phenylpropanoids(PP), C6-C3 compounds, are widely distributed in many plants. In this experiments, effect of PP on sheep red bood cells (sRBC)-induced delayed type hypersensitivity (DTH) were studied in ICR male mice. SRBC were challenged by i.p. injection at two weeks after sensitization of Lp. injection of sRBC. Five days after the challenge of antigen, paw edema induced 24 hours after the last challenge by DTH, respectively. (omitted)