• 대한한방소아과학회지
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• 제6권1호
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• pp.15-32
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• 1992

This study was done to know the effect of Giyuetang on the inflammatory response, contact hypersensitivity (CH), and rosette forming capacity of spleen cells. The effects of Jiyutang with Fructus Immaturus Ponciri on the inflammatory response were evaluated by measuring the production of such reactive oxygen intermediate(ROI) as $O_2^-$ and $H_{2}O_2$ in the peritoneal neutrophils and macrophages. The effects of Jiyutang with Fructus Immaturus Ponciri on the CH were evaluated by checking the ear swelling response against dinitrofluorobenzene(DNFB). The administration of Jiyutang with Fructus Immaturus Ponciri on the mouse decreased the amount of ROI in both neutrophils and macrophages. Jiyutang with Fructus Immaturus Ponciri depressed CH without affecting the rosette forming capacity of spleen cells. The results of this study showed that Jiyutang with Fructus Immaturus Ponciri might have anti-inflammatory effects by decreasing the amounts of ROI in the phagocytes and suppressing the CH, without affecting the rosette-forming capacity of spleen cells.

• 약학회지
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• 제35권2호
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• pp.123-130
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• 1991

Effects of Allolobophora caliginosatrapezoides (Ac) polysaccharide fractions on the inflammation and hypersensitivity were studied in vivo. It showed that Ac polysaccharide fractions have the significant inhibitory activities of inflammation and hypersensitivity; They inhibited significantly the carrageenin-induced paw edema and acetic acid-induced writhing syndrome. They also inhibited significantly the Arthus reaction and delayed hypersensitivity in the sheep red blood cell-sensitized mice in accordance with the inhibition of haemaglutinin titer, haemolysin titer, plaque-forming cells and rosette-forming cells. They also improved markedly the oxazolone-induced dermatitis in rats dose-dependently. As the above results, it exhibited that Ac polysaccharide fraction inhibited not only humoral immune response, but also cell-mediated immune response. It seemed that methanol and ether extracts have also another physiological active agents.

• 대한미생물학회지
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• 제22권2호
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• pp.163-166
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• 1987

BCG 감염마우스의 비장으로부터 BCG 항원에 대한 수용체를 가진 세포를 BCG 항원으로 감작시킨 면양 적혈구(SRBC)를 이용한 rosette 방법으로 분리하였다. SRBC에 흡착되는 적정 BCG 항원 농도를 알아보기 위하여 tannic acid(1:20,000)으로 전처리한 SRBC에 여러 농도의 BCG 항원으로 작용시킨 결과 $50\;{\mu}g/ml$의 BCG 항원으로 반응시킨 경우 가장 높은 rosette 형성을 관찰할 수 있었다. 비장세포와 항원처리 SRBC와의 작용시간에 따른 rosette 형성율은 1시간 후에 최고에 달하였고 시간의 경과한다 하여 증가하지 않았다. BCG 감염후 1주에서 8주까지 비장에서의 rosette 형성세포를 관찰한 결과 감염 $3{\sim}4$주 후부터 가장 높은 rosette 형성율을 보였으며 이때 rosette 형성세포는 전 비장세포중 $5{\sim}7%$를 차지하였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.126.1-126.1
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• 2003

Compound-A is a phenylpropanoid isolated from Arctium lappa fruit. In this experiments, effect of Compound-A on sheep red bood cells (sRBC) - induced delayed type hypersensitivity (DTH) were studied in ICR male mice and determined the Rosette Forming Cells (RFC). Two weeks after sensitization of i.p. injection of sRBC (4$\times{10}^8$ cells), ICR male mice were challenged by i.p. injection of sRBC (2\times{10}^8$ cells). Five days the challenge of antigen, paw edema induced twenty-four hours after the last challenge by DTH. (omitted)

• 미생물학회지
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• 제36권1호
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• pp.64-68
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• 2000

Agrocybe cylindracea으로부터 분리 배양한 균사체 배양액을 이용하여 균사체내 다당류(IP)와 균사체외 다당류(EP)를 분리하고, 동물실험을 통한 각 다당류의 면역증강활성에 관해 실험을 실시하였다. 탐식능에 대한 실험결과, IP와 EP 모두는 복강 침출 세포(PEC)에서 대조군에 비해 6.6-50%, 말초 임파구(ML)에서 67-167%, 비장(SP)에서 16-90%의 전반적으로 높은 탐식능을 나타냈다. 또한 항체 생성능 측정을 위한 plaque forming cell(PFC)와 rosette forming cell(RFC)의 실험결과에서 볼 때, PFC형성능은 대조군에 비해 EP투여 II군에서 46-50%, IP투여 I군에서 49-70%의 활성증가를 나타내었다. 또한 RFC형성능은 대조군에 비해 IP투여 I군에서 91%의 증가를, EP투여 I군에서 33%, EP투여 II군에서 43%의 활성증가를 나타내어 높은 항체생성이 기대되었다. Mitogen 활성은 농도 의존적으로 세포 증식능을 보였으며, IP와 EP를 비교할 때, EP는 IP에 비해 약2.5배의 세포증식 활성을 보였다.

• 한국식품영양학회지
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• 제10권4호
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• pp.533-538
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• 1997

감초에서 분리한 각각의 추출물(GHE, GME, GAE)을 BALB/c mice에 투여하여 탐식작용, 용혈 반형성, 용혈소 역가 측정 및 rosette 형성 실험을 통해 감초가 면역응답에 미치는 영역을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비자세포(spleen cells)는 CONrns에 비하여 각각의 약물 투여군이 Candida parapsilosis에 대한 효과가 다소 높은 경향을 나타내었지만, 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 GHE, GME 투여군 및 GAE 투여군이 높게 나타났다. 3. Rosette 형성에 대한 측정에서는 CON군에 비하여 GME, GAE 투여군이 높은 유의성을 나타내었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제49권4호
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• pp.233-238
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• 2023

Schwannomas exhibit histopathological variation that leads to diagnostic dilemmas, although less frequent in the oral cavity. We describe a case with unique histopathology and no relevant clinical history that adds to the breadth of literature on the diversity presented by Schwannoma. A 60-year-old female patient presented with a small dome-shaped, asymptomatic swelling on the alveolar ridge 6 years in duration. Histopathologically, it showed rich cellular pathology with a unique arrangement of tumor cells forming irregular rosettes. Each rosette presented with a central core of fibrin-collagenous material and the tumor cells were arranged on the periphery, exhibiting epithelioid change with evidence of mild cellular and nuclear pleomorphism. On immunohistochemical evaluation, the cells were strongly and diffusely positive for S-100 and negative for Ki-67. A diagnosis of benign Schwannoma with a rosette-like arrangement with epithelioid change was made. The case report emphasizes the risk of misdiagnosis and the importance of awareness regarding rare histopathological variants of Schwannoma.

• 대한한방종양학회지
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• 제6권1호
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• pp.47-65
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• 2000

In order to investigate antitumor and immune response effect by Hyangsapyungwisan after Sarcoma-180 cells and methotrexate were treatred each other, the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration($IC_{50}$), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by $5{\mu}g/well,\;2.5{\mu}g/well,\;1.25{\mu}g/well,\;and\;0.625{\mu}g/well$ of Hyangsapyung-wisan concentration inhibited cell viability significantly. $IC_{50}$ for cell viability was $11.59{\mu}g/well$. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(p<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(p<0.05). 8. Production of interleukin-2 was significantly increased(p<0.05). According to the above results, Hyangsapygwisan had prominent antitumor effects, and enhance both cellular and humoral immunity in mice.

• 대한수의학회지
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• 제29권4호
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• pp.525-530
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• 1989

In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody($EA_{Fc}$)- and erythrocyte-antibody-complement (EAC)-rosetting techniques. The results obtained were as follows; The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette ($6.7{\pm}2.4%$) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as $37.3{\pm}2.7%$ and $45.1{\pm}2.1%$, respectively. And the synergistic effects were noted no less than $66.5{\pm}5.6%$ when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3~24 hours incubation time at $4^{\circ}C$, EA-and EAC-RFR were $23.3{\pm}9.1%$ and $23.1{\pm}7.9%$, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.

• Archives of Pharmacal Research
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• 제15권1호
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• pp.20-29
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• 1992

Effects of squalene on cellular and non-specific immune responses and antitumor activity in mice were investigated. Cellular and non-specific immunological assay parameters adopted in the present study were delayed-type hypersensitivity reaction and resette forming cells (RFC) for cellular immunity, activities of natural killer (NK) cells and phagocyte for non-specific immunity. Squalene resulted in marked increases of cellular and non-specific immune functions and enhancement of host resistance to tumor challenge in dose-dependent manner.