• Journal of Aquaculture
• /
• v.18 no.4
• /
• pp.272-279
• /
• 2005

The efficacy of lidocaine hydrochloride and Clove oil as anaesthetics was evaluated in the Korean rose bitterling, Rhodeus uyekii (Mori, 1935) and oily bitterling, Acheilognathus koreensis (Kim and Kim, 1990) at four different temperatures of $10^{\circ}C,\;15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$. When complete anaesthesia was acquired less than 3 min and recovery was acquired less than 10 min, the optimal dose range of lidocain hydrochloride at $20^{\circ}C$ was 250${\~}$550 ppm in Korean rose bitterling, and 150${\~}$550 ppm in oily bitterling, respectively. In case of Clove oil, the optimal dose range at $20^{\circ}C$ was 40${\~}$200 ppm in Korean rose bitterling and 80${\~}$240 ppm in oily bitterling, respectively. Both of lidocaine hydrochloride and Clove oil resulted in a negatively dose-dependent manner for anaesthesia induction time in these two species. Recovery times were more variable in relation to anaesthetic doses, but in general higher anaesthetic doses resulted in similar or longer recovery time. As expected, the lower temperature resulted in longer anaesthesia induction and recovery time. The study demonstrated that lidocaine hydrochloride and Clove oil can be used as effective anaesthetics in these two species. The results from this study could be useful for aquaculturists industry and other related husbandry practices that require anaesthesia of Korean rose bitterling and oily bitterling.

• Development and Reproduction
• /
• v.19 no.3
• /
• pp.135-144
• /
• 2015

Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70 (RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70, HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.

• Fisheries and Aquatic Sciences
• /
• v.14 no.4
• /
• pp.429-433
• /
• 2011

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (${\tau}_0$). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between ${\tau}_0$ and water temperatures at all temperatures studied (Y = -1.225X + 70.05, $r^2=0.988$, where Y is ${\tau}_0$ and X is temperature).

• Development and Reproduction
• /
• v.18 no.4
• /
• pp.251-258
• /
• 2014

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.18 no.6
• /
• pp.586-593
• /
• 1985

The rose bitterling, Rhodeus ocellatus (KNER) is commonly distributed in the fresh waters of Korea and Japan. On January 15, February 15, and March 18 in 1984, mature adults of rose bitterling were caught in the watercourse of Maeri, Kimhae, South Korea. The authors fertilized the eggs employing dry method in the laboratory on May 7, 16 and 25 in 1984. Hatched larvae were reared in small aquariums at $17{\sim}25.5^{\circ}C$ (average around $21.2^{\circ}C$). Mollusks, Anodonta woodiana in the gill chamber of which, rose bitterling lay eggs were caught in order to study natural spawning of the rose bitterling in the same watercourse. The eggs of this species are not adhesive and demersal. The size of the eggs varies from 2.54 to 2.75mm in long diameter and 1.45 to 1.65mm in short diameter. The eggs are cylindrical in form when they are extruded from ovipositor, immediately after entering water, but they acquire their distinctive form of a greatly elongated pear. Hatching took place in ca. 39 hours after fertilization. The newly hatched larvae were $2.65{\sim}2.70mm$ in total length possessing yolk sac and 13-14 myotomes. Thirteen days after hatching, the prelarvae attained 6.5 mm in total length, and the first melanophores appeared on the head, and the anterior part and sides of the yolk sac. One month after hatching, the postlarvae attained 8.5mm. in total length and emerged from the gills of the mollusks. Then the yolk sac was completely resorbed. Two months after hatching, the rose bitterling attained 14.4mm in total length, and entered the fingerling period of life. All the rays already present were the D. III, 11-12, A. III, 11-12, P. 10, V. 7. and a distinguishing, feature is the presence of a black pigment spot in the lobe of the dorsal fin.

• Journal of Aquaculture
• /
• v.22 no.1
• /
• pp.100-104
• /
• 2009

This study was conducted to evaluate the effect of dietary supplementation of paprika powder as carotenoid source with three different zooplankton on carotenoid deposition and skin pigmentation of rose bitterling Rhodeus ocellatus. Two replicate groups of fish (initial average weight 0.8 g) were fed the five experimental diets containing 5% paprika (P5) and mixture of 5% paprika with 3% three zooplankton of water flea Moina macrocopa (P5M3), Artemia nauplius Artemia sp. (P5A3) and rotifer Brachionus plicatilis (P5R3) and without supplementation of carotenoid source (Con) for 10 weeks. Survival of fish among the treatments was not affected by dietary supplementation. Total carotenoid contents of whole body in fish fed the diets containing 3% zooplankton was higher than that of fish fed other diets. Especially, the highest total carotenoid contents of whole body was observed in fish fed the P5A3 diet. Similarity, Lightness, $a^*$ and $b^*$ values of the fish fed the diets containing 3% zooplankton tended to be higher than that in fish fed control and P5 diets. The apparent color intensity of skin and fin in fish fed the diets containing paprika with Artemia nauplius and rotifer was better than those of fish fed other diets. The results of this study suggest that dietary supplementation of paprika with Artemia nauplius and rotifer can improve skin pigmentation of rose bitterling.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.4
• /
• pp.520-524
• /
• 1999

The rose bitterling (Rhodeus uyekii), aboriginal fish of Korea, have beautiful nuptial colors on the body and fins in their spawning season. So, this species has high possibility to become a new ornamental Bish. However, nothing is known about the color composition of body colors of this species. Therefore, this study was conducted to investigate the color component of the skin and fins of the bitterling collected from natural environment. The major component of the nuptial colors was carotenoids according to the present study. Amount of total carotenoids on the skin and fins, had a tendency to be higher during the spawning season than the non-spawning season; it was $44.9mg\%$ during spawning season and $41.8mg\%$ during non-spawning season. The composition of carotenoid during spawning season was $36.7\%$ of zeaxanthin $30.1\%$ of $\beta$-carotene, and $16.4\%$ of diatoxanthin. The composition of carotenoid during non-spawning season was $41.2\%$ of $\beta$-carotene, $18.1\%$ of diatoxanthin, and $14.0\%$ of isocryptoxanthin. The major component of the carotenoid during spawning season turned out to be zeaxanthin while the major component of the carotenoid during non-spawning season was $\beta$-carotene.

• Development and Reproduction
• /
• v.20 no.4
• /
• pp.289-296
• /
• 2016

This report describes the sex differentiation of the Korean rose bitterling, Rhodeus uyekii, from hatching to 170 days post-hatch (DPH) in relation to total length (TL), body weight (BW), and integral water temperature (IWT). The growth curve of TL from just hatching to 83 DPH was $5.144e^{0.045t}$ ($R^2=0.961$; t, time), and that of BW was $2.398e^{0.086t}$ ($R^2=0.725$). Primordial germ cells (PGCs) were observed at 17 DPH (7.9 mm TL, 3.74 mg BW, $374^{\circ}C$ IWT), and thereafter began to protrude into the peritoneal cavity. At 21 DPH ($9.2{\pm}0.14mm$ TL, $4.8{\pm}0.07mg$ BW, $462^{\circ}C$ IWT), some PGCs contained condensed chromatin and oocyte were observed in meiotic prophase. In contrast to the ovaries, which grew gradually after sexual differentiation, testes began multiplying at 25 DPH (10.1 mm TL, 5.42 mg BW, $550^{\circ}C$ IWT), when testicular differentiation was first identified, and multiplied continuously thereafter. At 33 DPH (11.2 mm TL, 10.5 mg BW, $726^{\circ}C$ IWT), the developing testes contained spermatogonia that exhibited mitotic activity. No spermatocyte or sperm cell was observed until 83 DPH (18.9 TL, 48.2 mg BW, $1,826^{\circ}C$ IWT). At 170 DPH (32.5 mm TL, 270.1 mg BW, $3,740^{\circ}C$ IWT), which was the end point of this study, the mature ovaries showed germinal vesicle breakdown, while the mature testes contained observable spermatocytes and sperm cells. These results allow us to identify the sex differentiation type of the Korean rose bitterling as differentiated gonochoristic.

• Development and Reproduction
• /
• v.18 no.1
• /
• pp.33-41
• /
• 2014

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

• Korean Journal of Ichthyology
• /
• v.13 no.2
• /
• pp.111-116
• /
• 2001

Factors influencing elongation of the ovipositor in the bitterling Rhodeus uyekii were examined under several conditions. The ovipositors of the females were periodically elongated under the presence of mussels or males, and in some cases without either. When females were reared without mussels and males, the ovipositor did not elongate in one fish in a large aquarium, but it did become elongated in another fish in a small aquarium and in many fishes in a large aquarium. Duration of the elongated state of the ovipositor was 1~3 days (mean 1.2~2.5 days) and the periodicity of elongation was 4~15 days (mean 6.4~11.9 days). Length of the elongated ovipositor was 68~100% (mean 78.5~98.8%). Length of ovipositor in the interpeak period was 20~51% (mean 27.3~33.1%); in the post-elongation period it did not elongated further 3~30% (mean 11.1~19.9%). The effect of aquarium size and number of individuals in the aquarium on the elongation of the ovipositor, and inter-specific or inter-generic differences based on previous reports are discussed.