• Journal of Sasang Constitutional Medicine
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• v.1 no.1
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• pp.139-151
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• 1989

In order to investigate the effects of Raw, Dried and Steamed Roots of Rehmanniae Radix (R.R.: from Wonju province, Korea) on cell-mediated arid humoral immune response, the author performed this experimental study. Delayed type hypersensitivity (DTH) and rosette forming cells (RFC) for cell-mediated immune response, hemagglutinin (HA) titers, hemolysin (HL) titers were maeasured in ICR mice. The results were summarized as follows: 1) DTH was increased with the order of Steamed Roots of R.R., Raw Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. 2) RFC were increased with the order of Raw Roots of R.R., Steamed Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. 5) HA titers were increased with the order of Steamed Roots of R.R., Row Roots of R.R., Dried Roots of R.R.treated group, as compared with the control group, with statistical significance. 4) HL. titers were increased with the order of Raw Roots of R.R., Steamed Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. Through in the experimental study in ICR mice, these findings suggest that all of the treated group was increased in cell-mediated immune reaponse, Raw, Steamed Roots of R.R. were increased more as compared with the Dried Roots of R.R., and all of the treated group was increased in humoral immune response, Raw, Steamed Roots of R.R. were increased more as compared with the dried Roots of R.R.

• Plant Resources
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• v.6 no.1
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• pp.1-6
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• 2003

Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22 $^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

• KSBB Journal
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• v.4 no.3
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• pp.288-292
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• 1989

Induction and culture of hairy roots from ginseng(Panax ginseng C. A. Meyer) roots discs by A. rhizogenes strain $A_4$ were studied. After 6-12 weeks infected with A. rhizogenes tumor and hairy roots emerged from the root discs. The ratio of hairy root induction on root discs was higher in 5-year old than in 3, 4, and 6-year old ginseng. On treatment with IAA, IBA, 2, 4-D and tryptophan, hairy roots formation showed a significant increase at 15-30mg/1 tryptophan treated. Subsequently, hairy roots were cultured on hormone-free RCM medium(pH 4.5, sucrose 30g/1).

• Mycobiology
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• v.28 no.1
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• pp.27-32
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• 2000

The ectomycorrhizal roots were collected from the soils around the bases of basidiocarps of the four edible mushrooms in the stands of Pinus densiflora or Querus acutissima communities (Mt. Wol-Ak in Eastern Chung-Puk): The basidiocarps of Tricholoma matsutake (TM), Sarcodon asparatus (SA), S. imbricatum (SI), and Polyozellus multiplex (PM) are usually collected. The ectomycorrhizal roots of TM, PM and SI were related to the roots of P. densiflora, but the other to the roots of Q. acutissima in Korea. Particularly, the basidiocarps of PM were collected in the mixed stand of both P. densiflora and Q. acutissima. The morphologies of the ectomycorrhizal roots were observed to be the yellowish brown coral (dichromatous) or pyramid types in the roots of the pine, but dark brown un-branched sticks (roots) in the ends of ectomycorrhizal roots of Querus plants. The un-branched roots were covered with the dark mycelia (rhizomorph) around them. Therefore, the ectomyorrhizal roots of PM were observed to have two kinds of types; The single blackish un-branched roots were observed to be attached to the yellowish coral type roots. The bundles of TM mycelia were filled with cortical cells (in the roots of P. densiflora), but the mycelia of the other fungi (Aphylloporales) were massed between the cortical cells of P. densiflora or Q. acutissima. Their anatomical and gross features were considered to be simailar but very important in the ectomycorrhizal roots for these edible mushrooms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.3
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• pp.193-197
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• 1982

1t is well known that the stem is filed with shoot units in the rice plant and each internode bears several crown roots. But it has not yet been ascertained that what controls the differential elongation of the crown roots in the same internode. Thereupon, author had been carried out this experiment to ascertain what controls the elongation of the crown roots in the same internode, especially on the conception of sink-source by leaf-cutting method. Generally, one shoot unit has two important sinks: one axillary bud (tiller) and several crown roots. When we removed the axillary bud, namely shoot unit has one sink: several crown roots, the crown roots formed near the midvein (source) were longer than the crown roots born near the axillary bud. And when the shoot unit has two sinks: one axillary bud and several crown roots, the other way, the crown roots formed at the prophyll unit of the tiller were longest, and the crown roots formed near the midvein were shortest and the crown roots born the near the tiller showed interim length. Juding from the present results, we can suppose that, when shoot unit has two sinks, axillary bud is superior sink than the crown roots. So that axillary bud grows faster than crown roots and tiller becomes a new source. Therefore the crown roots which formed at the new source and the crown roots born the near the new source are longer than others.

• Korean Journal of Pharmacognosy
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• v.48 no.1
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• pp.51-55
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• 2017

Aralia continentalis Kitagawa (Araliaceae), known as "Dokwhal" in Korea, has been widely used in traditional Korean medicine for analgesia, neuralgia, sweating, and rheumatism. The biological activity was estimated with methanol extracts of from cultivated roots and adventitious roots of A. continentalis. DPPH and ABTS activities showed the highest activity in methanol extract of adventitious roots at 175.6 and $279.7{\mu}g/mL$ ($RC_{50}$), respectively. Antioxidant activity of methanol extract of the adventitious roots was higher than other samples. In the antibacterial activity assay (paper disc method), the methanol extract of adventitious roots showed activity against S. aureus, S. epidermidis, B. subtilis, and S. enterica. This study demonstrated that adventitious roots of A. continentalis, which was produced for the first time in the roots of A. continentalis, can be used as a natural antioxidant and antibacterial agents.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.3
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• pp.350-356
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• 2012

The purpose of this study was to make Korean yaksun chicken sauce with milk vetch roots and angelica roots. Four different amounts of angelica roots were added to chicken sauces, and general characteristics were measured. The pH values of sauces increased with decreasing Angelica root content. On the other hand, sugar contents, solid contents, and lightness of sauces decreased with decreasing Angelica root content. In a palatability test, sauces with 1 g and 2 g of angelica roots scored high in color, taste, flavor, and overall acceptability. Microbial cell counts of sauces were not significantly different according to the amount of angelica roots. Further, hydroxyl radical scavenging activities and polyphenol contents of sauces were not significantly different except for those of sauces with 1 g of angelica roots. Lastly, flavonoid contents of sauces significantly decreased with decreasing amount of angelica roots.

• Journal of Ginseng Research
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• v.21 no.3
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• pp.187-195
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• 1997

The Chinese ginseng roots were collected at twelve places of Jilin Province and two places of Liaoning Province in China and their appearances were compared with those of the Korean ginseng roots. The color of the most of the Chinese red ginseng was brown or dark brown and the color of many of the Chinese dried ginseng was pale yellow and the root-age of the most of the Chinese red ginseng as well as the Chinese dried ginseng was evaluated five or six year regardless of the collection places, so it cannot be easily concluded that the color and the root-age of the Chinese ginseng roots are different from those of the Korean ginseng roots. However the rhizomes and the lateral roots of the Chinese ginseng roots were poorly developed and many of them did not have either rhizome or lateral roots. Moreover the rhizomes of the Chinese red ginseng as well as the Chinese dried ginseng were much more easily removed than those of the Korean red ginseng and the Korean white ginseng. Therefore it is thought that the development status of the rhizome and the lateral roots of the Chinese ginseng roots are quite different from those of the Korean ginseng roots.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.151-162
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• 2001

Korea mountain ginseng known as oriental miracle drug is an important medicinal plant. The effect of mountain ginseng adventitious roots extract has been described. The valuable root of mountain ginseng contained several kinds of ginsenosides that have been confirmed to have many active functions for the human body. However, the study of mountain ginseng has a limit because the price of wild ginseng is very expensive and rare. The mountain ginseng adventitious roots were derived from mountain ginseng callus that were induced from mountain ginseng roots. Adventitious roots were separated from callus and grown in solid media(Murachige and stoog media). It was cultured in a 20L bioreactor. After culturing for 40days, adventitious roots were harvested. Afterwards the harvested mountain ginseng adventitious roots were dryed and extracted. We examined the effect on melanogenesis of mountain ginseng adventitious roots extrac. Here, we report the inhibitory effect of melanin biosynthesis on the adventitious roots extract of In vitro test. Also, we assessed the safety of adventitious roots extract. In vitro, cytotoxicity of adventitious roots extract was assessed in mouse fibroblast using two method: The neutral red uptake assay and the MTT assay. In vivo, the allergic and irritant were patch tested in 30 patients. Consequently, extract of mountain ginseng adventitious roots have inhibitory effect on melanin biosynnthesis in B-16 melanoma cell test, tyrosinase inhibitory test and DOPA auto-oxidation test. There were decreased 86%(0.5% concentration), 45%(1% concentration) and 61%(1% concentration), respectively

• Plant Biotechnology Reports
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• v.5 no.3
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• pp.255-263
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• 2011

Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of triterpenoid saponin with high medicinal values. In this work, we induced transgenic hairy roots of C. lanceolata and analyzed triterpenoid saponins from the hairy roots and hairy root-derived transgenic plants. Hairy roots were obtained from leaf explants by the transformation of Agrobacterium rhizogenes R1000. Transgenic hairy root lines were confirmed by the transcriptional activities of rolA, B, C, and D genes by RT-PCR. Transgenic root lines actively proliferated on hormone-free medium but not in nontransformed roots. Hairy roots contained richer triterpenoids (lancemaside A, foetidissimoside A, and aster saponin Hb) than nontransformed roots. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. They showed phenotypic alterations such as shortened shoots and an increased number of axillary buds and adventitious roots. The transgenic plants also contained higher triterpenoid levels than wild-type plants. These results suggest that hairy roots and transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.