• Plant Biotechnology Reports
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• v.5 no.3
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• pp.225-234
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• 2011

Arabidopsis mutants with T-DNA insertion in seven calmodulin genes (CAM) were used to determine the specific role of CAM in the tolerance of plants to oxidative stress induced by paraquat and hydrogen peroxide ($H_2O_2$) treatments. Arabidopsis calmodulin mutants (cam) were screened for seedling growth, seed germination, induced oxidative damage, and levels of ${\gamma}$-aminobutyric acid (GABA) shunt metabolites. Only the cam5-4 and cam6-1 mutants exhibited an increased sensitivity to paraquat and $H_2O_2$ during seed germination and seedling growth. In response to treatments with $3{\mu}M$ paraquat and 1 mM $H_2O_2$, only the cam5-4, cam6-1 mutants showed significant changes in malonaldehyde (MDA) levels in root and shoot tissues, with highly increased levels of MDA. In terms of the GABA shunt metabolites, GABA was significantly elevated in root and shoot tissues in response to the paraquat treatments in comparison to alanine and glutamate, while the levels of all shunt metabolites increased in root tissue but not in the shoot tissue following the $H_2O_2$ treatments. GABA, alanine and glutamate levels were significantly increased in root and shoot of the cam1, cam4, cam5-4, and cam6-1 mutants in response to paraquat (0.5, 1 and $3{\mu}M$), while they were increased only in the root tissue of the cam1, cam4, cam5-4, and cam6-1 mutants in response to $H_2O_2$ (200 and $500{\mu}M$, 1 mM). These data show that the cam5-4 and cam6-1 mutants were sensitive to the induced oxidative stress treatments in terms of seed germination, seedling growth, and oxidative damage. The accumulation of GABA shunt metabolites as a consequence of the induced oxidative stress treatments (paraquat and $H_2O_2$ treatments) suggests that the GABA shunt pathway and the accumulation of GABA metabolites may contribute in antioxidant machinery associated with reactive oxygen species and in the acquisition of tolerance in response to induced oxidative stress in Arabidopsis seedlings.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.264-273
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• 2016

This study was conducted to find out the influence of water stress from May to June on photosynthesis, shoot growth and fruit quality of apple trees. Fuji'/M.9 apple trees were root pruned at full bloom (30th April), parallel to the row of 30 cm or 60 cm from the trunk, to one side or both sides of trunk. The average value of afternoon leaf water potential from May to June of the no root pruning was maintained over -1.80 MPa, but that of root-pruning treatments was maintained under -1.80 MPa. In the comparison of average value of leaf water potential from May to June by root-pruning degree, the root pruning both sides at 30 cm from trunk was lowest (-2.06 MPa), followed by the root pruning both sides at 60 cm (-2.02 MPa) and the root pruning one side at 30 cm (-1.91 MPa). Root pruning one side at 30 cm or both sides at 60 cm reduced photosynthetic rate from May to June by 80% or 65%, respectively, compared with no root pruning. So, the degree of decreasing total shoot length or average fruit weight compare to the no root pruning of the root pruning one side at 30 cm was 25% or 11%, respectively, and those of the root pruning both sides at 30 cm was 45% or 15%, respectively. However, the soluble solid content and fruit color of the root pruning was higher than those of the no root pruning, and those of the root pruning both sides was higher than root pruning one side. These results indicate that the fruit enlargement was decreased when the average value of average leaf water potential from May to June was under -1.80 MPa, and the shoot growth was decreased when that was under -2.00 MPa.

• Journal of Forest and Environmental Science
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• v.26 no.3
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• pp.193-202
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• 2010

To analyze the effects of tree roots on the stability of damage slope, distributional and physical properties of five-year Pinus densiflora roots were investigated. In the composition of roots, the proportion of main root to lateral root was 1 to 9 in slope condition. Root tensile force was increased in accordance with increased proportion to diameter of Pinus densiflora roots. However, tensile strength was decreased in proportion to diameter of roots. Root shear strength showed that soil containing Pinus densiflora roots was higher than that of non-treated soils. This result shows that Pinus densiflora roots significantly stabilize the surface-soil rather than sub-soil in damage slopes.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.6
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• pp.1784-1797
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• 1996

The disk/blade assembly of a turbine engine is made in the shape of a dovetail type or a fir-tree type. Since disk fillet regions or contact surfaces undergo high stress comcentration, fatigue cracks frequentrly occur in the disk/blade assembly. Therefore, it is necessary to analyze the stress distributions in the fir-tree type disk/balde assembly and predict the region of fatigue failure. The stress distributions of the disk/blade assembly were investigated by using the photoelastic method and the finite element method. Two dimensional photoelastic techniques were used to investigate the stress distributions of contact surfaces and fillet regions. TH stress distributions were obtained by the shear-difference method and were compared to the finite element results. It was found that maximum tensile stresses were higher in the fillet region thatn in the contact surfaces of the fir-tree models. The finite element results showed good agreement with the experimental results.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.11
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• pp.1813-1819
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• 2001

Partial penetration welding Joint defines that groove welds without steel backing, welded from on side, and groove weeds welded from both sides but without back gouging, that is. it has an unwelded portion at the root of the weld. In this study we analysed fur residual stress and displacement distribution on partial penetration welding condition of thick plate metal. For 25.4mm thick plate, theoretical residual stress and displacement analysis by finite element method using ABAQUS was carried out and compared with the experimental result using hole-drilling method. In results of the condition of partial penetration, it appeared that longitudinal stress at welding area was a little difference and transverse stress did not have any effect by partial penetration multi-pass welding. From a point of welding distortion in partial penetration multi-pass welding, it seemed to be better to control root face smaller than 6.35mm.

• Journal of Welding and Joining
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• v.3 no.2
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• pp.52-63
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• 1985

Fillet welded joints, specially in ship structure, are well known the critical part where stress concentrate or crack initiates and grows. This paper is concerned with the study of the behavior of fatigue crack growth t the root and toe of load carrying cruciform fillet welded joints under three points bending by the determination of stress intensity factor from the J-Integral, using the Finite Element Method. The stress intensity factor was investigated in accordance to the variation of the weld size (H/Tp). weld penetration (a/W) and plate thickness (2a'/Tp). As mixed mode is occurred on account of shearing force under the three points bending, Stern's reciprocal theory is applied to confirm which mode is the major one. The main results may be summarized as follows 1) The calculation formula of the stress intensity factor at the both of root and toe of the joint was obtained to estimate the stress intensity factor in the arbitrary case. 2) The change of stress field around crack tip gives much influence on each other at the roof and toe as H/Tp decreases. 3) Mode I is a major mode under the three points bending.

• Proceedings of the KWS Conference
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• 2004.05a
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• pp.246-248
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• 2004

It has been well known that ductile fracture of steels is accelerated by triaxial stresses. The characteristics of ductile crack initiation in steels are evaluated quantitatively using two-parameters criterion based on equivalent plastic strain and stress triaxiality. This study provides the fundamental clarification of the effect of strength mismatching, which can elevate plastic constraint due to heterogeneous plastic straining, loading mode and loading rate on critical condition to initiate ductile crack from notch root using equivalent plastic strain and stress triaxiality based on the two-parameter criterion obtained on homogeneous specimens under static tension. The critical condition to initiate ductile crack from notch root for strength mismatched bend specimens under both static and dynamic loading would be almost the same as that for homogeneous tensile specimens with circumferential sharp notch under static loading.

• Proceedings of the KWS Conference
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• 2005.06a
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• pp.253-255
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• 2005

It has been well known that ductile fracture of steels are accelerated by triaxial stresses. The characteristics of ductile crack initiation in steels are evaluated quantitatively using two-parameters criterion based on equivalent plastic strain and stress triaxiality. The present study focuses on the effects of strength mismatching, which can elevate plastic constraint due to heterogeneous plastic straining, on critical condition to initiate ductile crack from notch root using equivalent plastic strain and stress triaxiality. In this study evaluate the criterion for ductile crack initiation in strength mismatch specimen effect of location of notch root.

• Journal of Welding and Joining
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• v.2 no.2
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• pp.62-69
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• 1984

In this study, cold crack susceptibility of high strength steel (ABS EH32 Steel) welded zone with shielded metal are welding was investigated by tensile restraint cracking test method. Effects of diffusible hydrogen content on root cracking, lower critical stress, crack initiation and fracture mode, hardness value distribution of welded zone and fractograph were mainly investigated. Following conclusions are made: 1. In the view of the lower critical stress level, wet electrode, containing much diffusible hydrogen content shows lower value than dried electrode. 2. Hardness value(Hv 5kg) in Heat Affected Zone of wet electrode is higher than that of dried electrode caused by hydrogen embrittlement. 3. In the case of wet electrode, root crack is initiated and propagated in Heat Affected Zone and then propagated to weld metal, but using of dried electrode, root crack is initiated in Heat Affected Zone and propagated to weld metal without propagating in HAZ. 4. For wet electrode, quasi-cleavage fracture mode is majorly observed on the fracture surface of HAZ and partially of weld metal due to hydrogen embrittlement.

• Weed & Turfgrass Science
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• v.4 no.3
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• pp.256-261
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• 2015

Trampling stress in turfgrass fields caused by traffics often occurs in zoysiagrass (Zoysia japonica) fields including golf course. In order to know the influences of trampling stress on the growth and development of turfgrass, leaf and root growth, chlorophyll fluorescence, chlorophyll content and 2-DE protein analysis were conducted in the turfgrass plants subjected to various levels of trampling stress from 0 to $9,420J\;day^{-1}$ day. Shoot growth was more highly inhibited by trampling stress than root growth. Although root growth was affected by trampling with weak intensity, the highest root length was observed in the turfgrass treated with weak trampling ($1,570J\;day^{-1}$). Chlorophyll fluorescence (Fv $Fm^{-1}$) was drastically lowered by trampling stress with moderate intensity. Leaf number showed similar tendency with leaf greenness. The number was decreased as the trampling intensity was increased. Shoot dry weight was decreased showing a similar tendency with the result of shoot length. The specific protein expressions under weak trampling were related to the functions of stress amelioration. Heat shock 70 kDa protein 10 and oxygen-evolving enhancer protein 1 were the proteins increased positively under trampling stress.