• 한국조경학회지
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• 제28권4호
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• pp.1-8
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• 2000

This study is conducted to evaluate the effects of root restriction on growth of maple(Acer palmatum Thumb.). Tow types of container such as clay pot(CP), root control bag(RCB) were used to restrict maple's root and each type of container was divided into 5 sizes. The containers with plants were buried just below the soil level and maples planted directly in the soil (nonrestricted root treatment) were included as comparison. Data were collected on dry weight of leaf, trunk, thick root, rootlet and soil water potential. We have analyzed, simple linear regression, Pearson's Correlation analysis, Duncan's multiple rang test, and Covariance Analysis using SAS statistical software. The results of analysis based on these data are as follows; 1. Total dry weight of maple in CP was significantly larger than in RCB. 2. Difference in growth reduction by the kinds of containers was induced by different hydraulic diffusion ratios between container and soil. Difference in growth reduction by the size of container was induced by the difference density of rootlet and soil moisture contents in the container. 4. Commercial products of root control of root control bag appeared not proper for countries in which fluctuation of rainfall is severe. Because maples in RCB were restricted by excess soil water in the rainy season, or by lack of soil water in the dray season.

• Restorative Dentistry and Endodontics
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• 제20권2호
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• pp.577-609
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• 1995

Bacteria have been regarded as one of the most important factors in pulpal and periapical diseases. Streptococci are frequently isolated facultative anaerobes in infected root canals. Recently molecular biological techniques have been rapidly progressed. This study was designed to apply the molecular biological tools to the identification and classification of streptococci in the endodontic microbiology. Streptococci isolated from infected root canals were identified with both Vitek Systems and API 20 STREP. Identification results were somewhat different in several strains of streptococci. Eighteen streptococci and enterococcal was difficult so to digest plasmid DNA using Hind III and EcoRI to differentiate strains by restriction enzyme analysis of plasmid DNA. 16S rDNA of chromosome was amplified by polymerase chain reaction(PCR) and then restricition fragment length polymorphism(RFLP) using several restriction enzymes was observed. The molecular mass of 16S rDNA of chromosomal DNA was approximately 1.4kb. There were three to five RFLP patterns using eight restriction enzymes. RFLP patterns digested with CfoI which recognizes four base sequences were identical in all stains. Hind III which recognizes six base sequences could not digest the 16S rDNA. Restriction enzymes which recognize five base sequences were suitable for RFLP pattern analysis. At least three different restriction enzymes were needed to compare each strains. 16S rDNA PCR-RFLP was simple and rapid to differentiate and classify strains and could be used in the epidemiological study of root canal infections.

• 원예과학기술지
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• 제33권4호
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• pp.511-517
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• 2015

'설향' 딸기 차근육묘에서 배지 및 차근자재의 종류가 자묘 생장에 미치는 영향을 구명하고자 본 연구를 수행하였다. 연구목적을 달성하기 위해 폴리에틸렌 필름이 차근자재인 조건에서 팽연왕겨, 사양토 및 양질사토 등 세 종류 배지가 자묘 생장에 미치는 영향을 밝혔다. 또한 폴리에틸렌 필름(PE), 부직포(NF), 유공비닐(PP) 및 방근시트(RPS)의 네 종류 차근자재를 설치하고 팽연왕겨를 배지로 충진한 후 배지의 함수량 및 근권온도 변화와 자묘의 생장에 미치는 영향도 구명하였다. 런너에서 발생한 자묘를 세 종류 배지에 고정시키고 20일 및 40일 후 조사한 자묘의 생장에서 팽연 왕겨 처리가 착근 20일 후의 엽 면적과 관부 직경, 그리고 40일 후의 관부 직경, 근중 및 생체중 등 지상부 및 뿌리 생육이 가장 우수하였다. 네 종류 차근 자재를 깔고 팽연왕겨를 충진한 후 관수 주기별 배지의 수분함량(volumetric water content, VWC)를 조사한 결과 PE 처리는 관수 후 55%로 측정된 VWC가 관수 직전 26%로 낮아졌으며, PP는 10-16%, NF 및 RPS는 관수 후 다음 관수까지 10% 미만의 VWC를 유지하였다. 육묘 기간 중 PE 처리가 NF, PP 및 RPS 처리보다 차근 자재에 영향을 받은 오후 2-5시 사이의 배지온도가 약 $1^{\circ}C$ 낮았고, 주 야간 온도차는 $1.5^{\circ}C$ 적었다. 자묘의 초장, 생체중 및 근 중은 PE 처리가 가장 크거나 무거웠다. NF 및 PP 처리는 뿌리가 차근되지 않아 자묘 뿌리가 차근자재 윗부분에 제한적으로 존재하지 못하고 자재 밑으로 많은 근계가 형성되었다. 이상과 같이 PE를 차근자재로 삼은 경우 팽연왕겨가 바람직한 배지이고, 차근 자재로써 PE가 NF, PP 및 RPS 보다 자묘의 생장에 더 우수한 영향을 미쳤으며, 본 연구 결과는 딸기 양질묘 생산을 위한 기초자료로 활용될 수 있을 것이다.

• Journal of Ginseng Research
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• 제14권2호
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• pp.310-316
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• 1990

This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular 9enetical approach of energy Production related mechanism in Panax ginseng. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. mtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not. Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRII treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN I and EcoRII. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector. Genomic library was screened and purified for further research including restriction mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned from the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.

• 생물환경조절학회지
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• 제8권1호
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• pp.36-41
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• 1999

본 연구는 토양근권제한이 멜론의 수량과 품질에 미치는 영향을 구명하고자, 격리상, 간이근권제한시트 및 관행 토양재배의 3가지 재배방법을 적용하여 멜론을 4월19일 부터 7월 17일까지 재배하였다. 지온은 격리상재배가 토양재배와 근권제한시트 재배보다 다소 높게 유지되었고 멜론의 생육은 토양과 근권제한시트재배에 비해 격리상 재배에서 빨랐다. 과실크기는 토양, 격리상 및 근권제한시트재배 순이었으나 당도 및 네트형성은 격리상재배가 토양재배에 비해 우수하였다. 수확기 경엽의 시들음주율은 토양재배 26.7%, 근권제한시트재배 25% 발생한 데 비해 격리상재배는 3.3%로 가장 낮았다 따라서 상품과 수량은토양재배에 비해 격리상재배에서 많았다.

• 한국환경과학회지
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• 제25권3호
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• pp.337-343
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• 2016

The present study aimed to determine the influence of various root restriction media on seedling quality and early growth of strawberry after transplanting. The root activity of the seedlings, measured 20 days after fixation, was considerably higher (0.096, 0.090, and $0.063mg{\cdot}g^{-1}{\cdot}h^{-1}$ at 420, 450, and 480 nm, respectively) in expanded rice hull (ERH) treatment than in the sandy loam and loamy sand treatments. The volumetric water content (VWC) of the root media tested across 3 irrigation regimes (15 d, 30 d, 45 d) in the nursery field was highest in sandy loam (65.0-66.8%), followed by 59.4-61.3% in loamy sand and 38.6-45.3% in ERH. When growth parameters of runner plantlets were compared, ERH treatment was found to result in the highest crown thickness and fresh weights of root and above-ground parts. This had a favorable influence on above-ground tissue growth after transplanting to plastic house soil. As mentioned above, ERH treatment resulted in the highest seedling quality and early growth after transplanting. The results of this study would serve as useful on-site data for the production of high-quality strawberry seedlings.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.168-174
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• 1990

This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular genetically approach of energy Production related mechanism in Panax Ein.fend. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. MtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRll treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN 1 and EcoRll. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector Genomic library was screened and purified for further research including restricttion mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned koto the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.

• Restorative Dentistry and Endodontics
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• 제22권1호
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• pp.413-427
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• 1997

Porphyromonas endodontalis and Prevotella intermedia are black-pigmented anaerobic gram negative rods which have been isolated from infected root canals and submucous abscesses of endodontic origin. And they are associated with clinical symptoms such as pain, percussion, and foul odor. It has been reported that there are 3 serotypes according to capsule membrane in P. endodontalis and 2 DNA homology groups and 3 serotypes in P. intermedia, but there is no data available regarding genetic diversity for the species P. endodontalis and P. intermedia. The purpose of this study is to investigate genetic diversities between individual strains of P. endodontalis and P. intermedia which are indistinguishable by serotyping and biotyping using bacterial DNA restriction endonuclease analysis. 45 teeth with at least one clinical symptoms, with single canal, and with pulp necrosis were sampled. For sampling bacteria, access cavity was prepared after disinfecting tooth and its surroundings. Then the paper point was inserted to the apex of the canal, leave there for 15 seconds, and finally it was placed into PRAS Ringer's solution and PBS solution. P. endodontalis and P. intermedia were identified by biochemical test and IIF after subculturing black and brown colonies which were produced after 7 days of incubation on BAP in anaerobic chamber. P. endodontalis and P. intermedia strains were grown in BHI broth and whole genomic DNA was extracted by phenol-chloroform extraction technique and digested by restriction endonuclease, Eco RI and Pst I. The resulting DNA fragments were separated by agarose gel electrophoresis, stained with EtBr and photographed under UV light. The results were as follows : 1. In both P. endodontalis and P. intermedia, different serotypes could be found within a root canal of same patient. 2. There were obvious genetic heterogeneity within a patient and within a serotype in both P. endodontalis and P. intermedia. 3. P. endodontalis serotype c, isolated from different patients, exhibited limited genotypic diversity.

• 한국정밀공학회지
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• 제16권10호
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• pp.187-193
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• 1999

Dimensional difference by welding deformation is usually occurred at steel bridge manufacturting or multi-pass welding used at joining of thick plates. Be this, root gap out of standard is frequently developed at butt weld joints. For example, although standard root gap below 6mm at butt weld joints of plates under 15mm thickness, maximum 30mm root opening is developed at the weld field. At this case, 24mm parts out of standard is generally built up. But, there are no accumulated data and restriction about this built up welding pars. In this study, therefore, the accumulation of data for built up parts and the verification of the mechanical properties of weld part as root gap is performed. It is included that tensile, bending, impact, hardness test and microstructural review for each welding specimen of 0mm, 6mm, 30mm root opening.

• 원예과학기술지
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• 제28권3호
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• pp.415-422
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• 2010

상추 2종, 쑥갓, 청경채, 엔디브, 그리고 치커리의 엽채류담액 수경 재배 시 높이 100mm, 직경 20, 25, 30mm의 원통형의 플라스틱 튜브를 이용하여 근권제한 처리를 한 후 생육과 식물영양소를 조사하였다. 근권제한은 용기 크기에 따라 엽채류의 지상부 생체중이 25-95% 범위에서 감소하였으며, 쑥갓과 청경채가 가장 영향을 많이 받았고 엔디브나 치커리는 적게 받았다. 건물률, C/N율 및 총비타민C와 총안토시아닌의 함량은 근권제한에 의해 증가하였다. 무기성분 중 P와 K함량은 근권제한에 의해 모든 작물에서 감소하였지만, Mg, Fe, Mn, Zn 등은 작물에 따라 차이가 있었다. 식물 영양적 가치를 증가시키고 생육 억제를 감소하기 위한 적절한 근권 용적은 엽채류 종류에 따라 달랐다. 치커리, 엔디브, 그리고 상추는 ${\Phi}25mm{\times}100mm$, 쑥갓과 청경채는 ${\Phi}30mm{\times}100mm$ 튜브 처리에서 각각 가장 좋은 결과를 보였다. 이상의 결과, 수경재배 시 원통형의 플라스틱 튜브를 이용한 근권제한 처리가 엽채류의 영양적 가치를 향상시킬 수 있다는 것을 시사하였다.