• Title/Summary/Keyword: root propagation

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Adventitious Bud Formation and Mass Propagation by Root and Propagation Medium of Wild Polygonatum odoratum Druce (야생 둥굴레의 뿌리와 배양토에 따른 대량번식과 부정아 발생)

  • 신동영;박종산;정연권;김학진;권병선;현규환
    • Korean Journal of Plant Resources
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    • v.15 no.1
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    • pp.8-17
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    • 2002
  • To improve new propagation method, I try to propagate wild polygonatum odoratum roots which formate adventitious bud without bud. The obtained results of size of roots and adventitious bud to formate for multiple propagation of wild polygonatum odoratum as the follow. One year grown wild polygonatum odoratum root in 5cm diameter formate 66.5% adventitious buds. One year grown wild polygonatum odoratum root in 15cm in diameter germinate 113% adventitious bud. Two years grown root in 15cm diameter formate adventitious bud three times more then one year root. Wild polygonatum odoratum in 15cm diameter without any bud in experiment plot makes 4 times of propagation. Sand and Perlite media propagaton plot was increased 3.1∼3.3times more than common culture medium. The best result of mass propagation wild polygonatum odoratum is 10mm diameter root and 5cm cut in length to plant sand media plot.

A Study on the fracture Mechanical Behavior of Cruciform Welded Joint With Fracture Cracks (십자형 필렛 용접 이음의 피로균열 에 대한 파괴 역학적 고찰)

  • 엄동석;강성원;유덕상
    • Journal of Welding and Joining
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    • v.1 no.1
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    • pp.37-46
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    • 1983
  • This paper describes a study of fillet welded joint stressed perpendicular to the weld line. The finite element method was used to determine the stress intensity factor for cruciform joint at weld toe and root cracks according to variation of H/Tp, weld angle and main plate thickness. But, in this study, weld angle was fixed at 45.deg., since the variation of weld angle affect the stress intensity factor little, also main plate thickness was fixed. Pulsating tension fatigue test was done at the second phase of experiment. The work using the concepts of the fracture mechanics on the stable crack growth, was in the correlation of the experimental fatigue stress-life behavior because the fatigue behaviors of various joint geometries are related to the stress intensity factors calculated by F.E.M. analysis. Main results obtained are summarized as follows. 1) According to the propagation of toe crack, the variation of the stress intensity factor at root crack is obvious as H/Tp is smaller. 2) According to the propagation of root cracks, the change of the stress intensity factor of the toe is very large with propagation of root crack. 3) The calculation formula of the stress intensity factor of crack propagation at the root crack was obtained. 4) The calculation formula of the stress intensity factor at the toe cracks was obtained in similar manner. 5) From the results of experiment, the velocity of fatigue crack propagation at the weld toe and root was estimated.

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생활치수치와 근관치료된 치아의 수직치근파절에 관한 증례보고

  • Lee, Se-Jun;Go, Seung-O;Jang, Mun-Taek
    • The Journal of the Korean dental association
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    • v.40 no.7 s.398
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    • pp.542-547
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    • 2002
  • This study was aimed to help diagnose the vertical root fractures in endodontically and in nonendodontically treated teeth. This was a study about mine teeth with a vertical root fractures. Four cases were nonendodontically treated teeth and five cases were endodontically treated teeth. A collection of information including past dental history, periodontal probing, and X-ray was gathered on each case. One of the endodontic cases had no periodontal pocket depth and was in acute pain. It was confusing to find whether causes were because of endodontic failure or vertical root fractures. Most of the vertical root fractures were found in patients aged between 50 and 60. Vertical root fractures were found in the molar and premolar of maxillary and mandibular teeth. The second molars repecially, which had C shaped roots had vertical root fractures with apical propagation type. The apical propagation type means that vertical root fracture initiates in root apex and propagate to coronal root. The teeth with vertical root fractures of the apical propagation type, which was in acute pain, were very difficult in differential diagnosis of periodontal lesion and endodontic failure. Therefore, the dental history was very important to make a differential diagnosis. Past pain history repeated uncomfortable symptoms in bite was a key of differential diagnosis.

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Root Induction and Propagation of Sedum takesimense Nakai Using Leaf Cutting Method

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.50-52
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    • 2018
  • The effect of basal nutrients concentrations and exogenous auxin for root induction from leaves of Sedum takesimense were investigated for mass-propagation. Root induction rates were significantly different from the concentrations of basal salts but not influenced by supplemented IBA in the medium. The lowest concentration of MS basal salts (1/10) was most effective to induce roots from leaves followed 1/5 MS, and 1/2 and full strength MS medium. Supplement of IBA $10{\mu}M$ in the medium did not improve the root induction that resulted no differences compare to the hormone free media. Rooted leaves were transplanted in soil and survived in greenhouse.

Propagation of Three Rubus Species Native to Korea by Stem Cutting

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • v.34 no.2
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    • pp.173-175
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    • 2018
  • Stem cuttings of three Rubus species native to Korea were treated by two commercial root stimulation formula for propagation. Differences among the species was obvious, toot was induced relatively easily on R. hongnoensis. Fifty percent of stem rooted without any treatment on this species and two stimulators was effective to increase the root induction. On the other hand, two other species, R. schizostylus and R. ribisoideus, had very low percent of rooting. Treatment using root stimulators was effective but still lower than that of R. hongnoensis. Root induction was not influence by the medium that resulted no differences between two media.

In vitro Micropropagation and Root Induction of Pear Genetic Resources

  • Jae-young Song;Jinjoo Bae;Woohyung Lee;Jung-ro Lee;Munsup Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.63-63
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    • 2022
  • Pear (Pyrus spp.) is a typical fruit and grown in the temperate climate regions throughout the world. Development of appropriate methods for in vitro propagation and root induction are important to increase the production rate and plant quality rapidly. This study was conducted to find the most appropriate media conditions for in vitro propagation and rooting of three pear cultivars, 'Barttlett', 'BaeYun No.3' and 'Oharabeni'. In vitro propagation was induced on Murashige and Skoog medium (MS) with 2.0 mg/L N6-benzyladenine (BA) and 0.2 mg/L indole-3-butyric acid (IBA) medium. For root induction of these cultivars, the shoot explants of the propagated plants were cultured on two different media containing 1/2 MS medium containing 0.2 mg/L IBA with 15 g/L Sucrose (Rooting Medium 1, RM1) and 1/4 Linsmaier and Skoog medium (LS) medium containing 1 mg/L IBA and 1 mg/L NAA hormone with 7.5 g/L glucose (Rooting Medium, RM2) and after 2 weeks, the plants on the RM2 medium are transferred on RM1 medium (RM2 condition). After nearly seven weeks, percentage of rooting formation were 22.2% in RM1 and 30% in RM2 conditions for Barttlett and 70% in RM1 and 60% in RM2 conditions for Oharabeni cultivars. No differences in these cultivars were observed between RM1 and RM2 conditions. However, BaeYun No.3 cultivar was observed 0% in RM1 and 72.7% in RM2 conditions. This study will help to propagation and root induction of in vitro plants for various pear cultivars.

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In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

  • Lim, Jong-Gu;Junzo, Fujigaki
    • Plant Resources
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    • v.7 no.3
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    • pp.222-225
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    • 2004
  • In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

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Incidence of apical crack formation and propagation during removal of root canal filling materials with different engine driven nickel-titanium instruments

  • Ozyurek, Taha;Tek, Vildan;Yilmaz, Koray;Uslu, Gulsah
    • Restorative Dentistry and Endodontics
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    • v.42 no.4
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    • pp.332-341
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    • 2017
  • Objectives: To determine the incidence of crack formation and propagation in apical root dentin after retreatment procedures performed using ProTaper Universal Retreatment (PTR), Mtwo-R, ProTaper Next (PTN), and Twisted File Adaptive (TFA) systems. Materials and Methods: The study consisted of 120 extracted mandibular premolars. One millimeter from the apex of each tooth was ground perpendicular to the long axis of the tooth, and the apical surface was polished. Twenty teeth served as the negative control group. One hundred teeth were prepared, obturated, and then divided into 5 retreatment groups. The retreatment procedures were performed using the following files: PTR, Mtwo-R, PTN, TFA, and hand files. After filling material removal, apical enlargement was done using apical size 0.50 mm ProTaper Universal (PTU), Mtwo, PTN, TFA, and hand files. Digital images of the apical root surfaces were recorded before preparation, after preparation, after obturation, after filling removal, and after apical enlargement using a stereomicroscope. The images were then inspected for the presence of new apical cracks and crack propagation. Data were analyzed with ${\chi}^2$ tests using SPSS 21.0 software. Results: New cracks and crack propagation occurred in all the experimental groups during the retreatment process. Nickel-titanium rotary file systems caused significantly more apical crack formation and propagation than the hand files. The PTU system caused significantly more apical cracks than the other groups after the apical enlargement stage. Conclusions: This study showed that retreatment procedures and apical enlargement after the use of retreatment files can cause crack formation and propagation in apical dentin.

Fatigue Strength and Root-Deck Crack Propagation for U-Rib to Deck Welded Joint in Steel Box Girder

  • Zhiyuan, YuanZhou;Bohai, Ji;Di, Li;Zhongqiu, Fu
    • International journal of steel structures
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    • v.18 no.5
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    • pp.1589-1597
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    • 2018
  • Fatigue tests and numerical analysis were carried out to evaluate the fatigue performance at the U-rib to deck welded joint in steel box girder. Twenty specimens were tested corresponding to different penetration rates (80 and 100%) under fatigue bending load, and the fatigue strength was investigated based on hot spot stress (HSS) method. The detailed stress distribution at U-rib to deck welded joint was analyzed by the finite element method, as well as the stress intensity factor of weld root. The test results show that the specimens with fully penetration rate have longer crack propagation life due to the welding geometry, resulting in higher fatigue failure strength. The classification of FAT-90 is reasonable for evaluating fatigue strength by HSS method. The penetration rate has effect on crack propagation angle near the surface, and the 1-mm stress below weld toe and root approves to be more suitable for fatigue stress assessment, because of its high sensitivity to weld geometry than HSS.

Adventitious root culture for secondary metabolite production in medicinal plants: A Review

  • Rahmat, Endang;Kang, Youngmin
    • Journal of Plant Biotechnology
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    • v.46 no.3
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    • pp.143-157
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    • 2019
  • Medicinal plants are high-value natural resources that have been used as precautionary drugs by many people globally. The increasing global demand for bioactive compounds from medicinal plants has led to the overexploitation of many valuable species. One widely used approach to overcome this problem is the use of adventitious root cultures as a propagation strategy. This review examines the scientific research published globally on the application of adventitious root cultures for many medicinal plants. Adventitious roots generated under aseptic environments in suitable phytohormone-augmented medium exhibit high growth rates and production of important secondary metabolites. Parameters such as medium properties and composition, growth hormone type, and elicitation strategies for in vitro grown adventitious roots of medicinal plants, are the main topics discussed in this review. We also examine current developments in bioreactor system cultivation for plant bioactive compounds using adventitious root cultures, a technology with possible commercial applications, via several studies on adventitious root culture of medicinal plants in which bioreactor systems play a role. In conclusion, the development of adventitious root cultures for medicinal plants is highly useful because of their capability for vegetative propagation and germplasm preservation.