• Title/Summary/Keyword: root pattern

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Colonizing Pattern of Fluorescent Pseudomonads on the Cucumber Seed and Rhizoplane (형광성 Pseudomonas屬 균주의 오이 종자처리 후 근권에서의 밀도와 정착양상)

  • Kang, Ji-Hyo;Park, Chang-Suk
    • Korean Journal Plant Pathology
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    • v.13 no.3
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    • pp.160-166
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    • 1997
  • Number of bacterial isolates were collected from high mountainous areas at various locations in the whole country to select promising biocontrol agents. Most of selected isolates belonged to fluorescent pseudomonads. Population densities of fluorescent pseudomonads were examined by DLF method. Alll selected isolates rapidly multiplied on spermosphere after seed inoculation upto 24, and then the population abruptly declined. When seeds were germinated fully, bacteria moved to newly emerging radicle. The good root colonizing isolates, B16 and V13 proliferated on the growing root and moved down to the root tip and lateral roots. but the poor root colonizing isolates, MC07 and X01 moved much slower. Scanning electron microscopic observations showed that the cells of the good colonizing isolates were arranged linearly on the growing root and parallel to growing root axis and continuously existed on the root tip, Whereas the cells of poorly colonizing isolates were gathered and scattered randomly on the root surface.

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Overexpression of three related root-cap outermost-cell-specific C2H2-type zinc-finger protein genes suppresses the growth of Arabidopsis in an EAR-motif-dependent manner

  • Song, Sang-Kee;Jang, Hyeon-Ung;Kim, Yo Han;Lee, Bang Heon;Lee, Myeong Min
    • BMB Reports
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    • v.53 no.3
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    • pp.160-165
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    • 2020
  • The root meristem of Arabidopsis thaliana is protected by the root cap, the size of which is tightly regulated by the balance between the formative cell divisions and the dispersal of the outermost cells. We isolated an enhancer-tagged dominant mutant displaying the short and twisted root by the overexpression of ZINC-FINGER OF ARABIDOPSIS THALIANA1 (ZAT1) encoding an EAR motif-containing zinc-finger protein. The growth inhibition by ZAT1 was shared by ZAT4 and ZAT9, the ZAT1 homologues. The ZAT1 promoter was specifically active in the outermost cells of the root cap, in which ZAT1-GFP was localized when expressed by the ZAT1 promoter. The outermost cell-specific expression pattern of ZAT1 was not altered in the sombrero (smb) or smb bearskin1 (brn1) brn2 accumulating additional root-cap layers. In contrast, ZAT4-GFP and ZAT9-GFP fusion proteins were distributed to the inner root-cap cells in addition to the outermost cells where ZAT4 and ZAT9 promoters were active. Overexpression of ZAT1 induced the ectopic expression of PUTATIVE ASPARTIC PROTEASE3 involved in the programmed cell death. The EAR motif was essential for the growth inhibition by ZAT1. These results suggest that the three related ZATs might regulate the maturation of the outermost cells of the root cap.

Push-out bond strength and intratubular biomineralization of a hydraulic root-end filling material premixed with dimethyl sulfoxide as a vehicle

  • Ju-Ha Park;Hee-Jin Kim;Kwang-Won Lee;Mi-Kyung Yu;Kyung-San Min
    • Restorative Dentistry and Endodontics
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    • v.48 no.1
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    • pp.8.1-8.8
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    • 2023
  • Objectives: This study was designed to evaluate the parameters of bonding performance to root dentin, including push-out bond strength and dentinal tubular biomineralization, of a hydraulic bioceramic root-end filling material premixed with dimethyl sulfoxide (Endocem MTA Premixed) in comparison to a conventional powder-liquid-type cement (ProRoot MTA). Materials and Methods: The root canal of a single-rooted premolar was filled with either ProRoot MTA or Endocem MTA Premixed (n = 15). A slice of dentin was obtained from each root. Using the sliced specimen, the push-out bond strength was measured, and the failure pattern was observed under a stereomicroscope. The apical segment was divided into halves; the split surface was observed under a scanning electron microscope, and intratubular biomineralization was examined by observing the precipitates formed in the dentinal tubule. Then, the chemical characteristics of the precipitates were evaluated with energy-dispersive X-ray spectroscopic (EDS) analysis. The data were analyzed using the Student's t-test followed by the Mann-Whitney U test (p < 0.05). Results: No significant difference was found between the 2 tested groups in push-out bond strength, and cohesive failure was the predominant failure type. In both groups, flake-shaped precipitates were observed along dentinal tubules. The EDS analysis indicated that the mass percentage of calcium and phosphorus in the precipitate was similar to that found in hydroxyapatite. Conclusions: Regarding bonding to root dentin, Endocem MTA Premixed may have potential for use as an acceptable root-end filling material.

Characteristics of Tamarix chinensis Population in Shiwha Lake (시화호 내 위성류(Tamarix chinensis) 개체군의 특성)

  • Min, Byeong-Mee;Yi, Dong-Hoon;Lee, Hye-Won;Choi, Jong-In
    • The Korean Journal of Ecology
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    • v.28 no.5
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    • pp.327-333
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    • 2005
  • For the first time in Korea, plant size, distribution pattern and root growth form of Tamarix chinensis were surveyed at natural stand in Shiwha Lake, from June to July in 2005. T, chinensis grew in the range of 350 m towards north-south and 270 m in east-west direction. The mean number of stems germinated from same root system was 1.9. On the stem base, the numbers of living and dead plants were 1,398 and 114, respectively. Mean perimeter and height of 1,398 plants were $5.9{\pm}3.2$ cm and $159{\pm}51$ cm, respectively. By dividing into 10 classes based on the perimeter size, the largest number of plants were included in the second smallest class category. However, by dividing into 10 classes on the bases of plant height, variation in the number of plants along the height showed normal distribution curve. The heights of dead plants were small in perimeter but diverse in height. On the root base, the number of plants was the greatest in the smallest size class, and conspicuously decreased with size increment. Distribution of T. chinensis was clumped pattern. Age of the largest plant was 8-year. T. chinensis had two types of root growth. One was horizontal growth in the periphery of soil surface, and the other vertical growth.

An Analysis of the Contrast Patterns of Lumbar Transforaminal Epidural Injection (요추 경추간공 경막외강 약물주입 시 조영상의 분석)

  • Kim, Sae Young;Han, Kyung Ream;Kim, Chan
    • The Korean Journal of Pain
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    • v.21 no.3
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    • pp.217-223
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    • 2008
  • Background: Lumbar transforaminal epidural injections (LTEIs) have been utilized in the treatment of radicular pain, and LTEIs have the advantage of target-specificity. However, there have not been enough studies on the contrast patterns in LTEIs with using fluoroscopy. The purpose of this study was to evaluate the spreading epidural contrast patterns that are seen during real-time fluoroscopic guided LTEIs. Methods: A total of 131 patients who underwent fluoroscopic guided LTEIs were studied. The inclusion criteria were those patients with low back pain and/or lower extremity pain that was caused by a herniated nucleus pulposus, lumbar spinal stenosis, failed back surgery syndrome, and herpes zoster-associated pain. We classified the contrast patterns in regard to the contrast flow spreading to the nerve root and/or the unilateral, bilateral or cylinderic type of epidural spreading on the AP view of the fluoroscopy and the ventral or dorsal epidural filling on the lateral view. In addition to the pattern analysis, we evaluated the range of contrast spreading from the cranial to the caudal epidural filling and the incidence of an intravascular flow pattern. Results: Epidural spreading was seen in 126 cases (96.2%) of the total patients through the nerve root. Ventral spreading occurred in 120 cases (95.2%). On the AP view, a nerve root with unilateral, bilateral and cylinderic epidural filling was noted for 108 (85.7%), 9 (7.1%) and 9 (7.1%) cases, respectively. The contrast spreading to vertebral segments was smaller for the patients with lumbar spinal stenosis and failed back surgery syndrome than for the other groups (P < 0.0083). The incidence of intravascular injection was 11.1% (14/126). Conclusions: LTEIs using fluoroscopic visualization provided excellent assessment of the ventral epidural filling as well as nerve root filling. However, unilateral epidural spreading was prominent for the LTEIs.

Anatomical Studies on the Classification of Cultivated Peony in Korea (재배작약의 분류에 관한 해부학적연구)

  • Yu, Seung-Jo
    • Korean Journal of Pharmacognosy
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    • v.1 no.3
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    • pp.81-92
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    • 1970
  • So far, the cultivated peony is known to be originated from an indigenous species, Paeonia albiflora $P_{ALLAS}$ var. trichocarpa $B_{UNGE}$ (PAT). In this study, these two species were morphologically examined in the external and internal feature and in the pattern of callus formation by tissue culture. Also, they were compared with another indigenous species, P. japonica $M_{IYABE}$ et $T_{AKEDA}$ var. pilosa $N_{AKAI}$ (PJ), which were regarded as being scarcely related to them. The root of the cultivated peony is massive consisting with several storage roots, each of them is a hypotrophic and fusiform. The root of PAT consists of several storage roots, each of them is branching and slender. And the storage root of PJ is short, bended buried horizontally, protruding a number of corpulent lateral root. The secondary xylem of the cultivated peony is small clusters of vessels and xylem fibres are arranged in scalariform and among these cluster, single vessel is joined, but that of PAT is small clusters of vessels are arranged in separate scalariform but are not connected with each other and that of PJ is vessels and xylem fibers are grouped together in elongated clusters that radiate outward from the center. Protoxylem of the cultivated peony is surrounded by four large metaxylem, but that of PAT and PJ by seven. On the other hand, the callus formation patterns of these peonies were different; the cultivated peony callus is formed in an orderly fashion by the mammalate meristematic cell groups, PAT callus is in disorder by the meristematic cells arranged in linear, and PJ callus is in order by the meristematic cells arranged in linear. By the comparison of three different plants in the anatomical appearance and the callus formation pattern, it is evident that the cultivated peony is not derived from PAT.

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Growth Pattern and Content of Tropane Alkaloids of Metabolic Engineered Scopolia parviflora Hairy Root Lines

  • Kang, Young-Min;Min, Ji-Yun;Kim, Won-Jung;Kim, Yong-Duck;Lee, Byung-Hynn;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.123-128
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    • 2004
  • Hyoscyamine and scopolamine are two most common tropane alkaloids found in the Solanaceae. The pEB expression vector carrying Nspmt gene was transformed to Agrobacterium rhizogenes. The growth of transgenic hairy roots were approximately to 80% of the wild type root. Transgenic hairy roots are less developed on only their branch roots than those of the wild type root. The extracts from Nspmt transgenic hairy root lines, 3 and 5 contained between 3.52 and 4.23 mg/g dry weight as hyoscyamine and did between 5.23 to 6.40 mg/g dry weight as scopolamine. These results showed that the overexpression of the pmt gene enhanced tropane alkaloids production of S. parviflora transformed roots and this improvement affected both hyoscyamine and scopolamine production.

Varietal Response to Phenoxy Herbicides on Plant Height and Root Length in Rice Plant (수종의 Phenoxy 제초제에 대한 수도품종별 초장(草長) 및 근장(根長) 반응(反應)에 관하여)

  • Park, Hoon;Kim, Moo-Sung;Ohh, Seung-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.1
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    • pp.55-62
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    • 1979
  • The effects of propanil, MCPA; 245-T and silvex on growth pattern were tested on Taichung Native 1. Caloro, PI 245717 and Bluebonnet 50 by root administration and foliar spray at 10 levels of concentration. Silvex in foliar spray increased plant height of Taichung and when applied via root decreased root langth of Bluebonnet and pi245717 at all levels. All phenoxy herbicides over 4 ppm - decreased plant height in all varieties tested when applied to root. General morphorogical response - were also observed.

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A HISTOLOGICAL STUDY OF THE ROOT RESORPTION APPLYING TO INTERMITTENT AND CONTINUOUS FORCE FOR INCISOR INTRUSION OF DOG (성견의 전치부 INTRUSION시 간헐적 교정력과 지속적 교정력의 적용에 따른 치근 흡수에 관한 실험적 연구)

  • Chang, Ji-Chul;Park, Young-Chul
    • The korean journal of orthodontics
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    • v.22 no.1
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    • pp.241-251
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    • 1992
  • The purpose of this study was to investigate the root resorption pattern in incisors in dog under intrusive orthodontic loadings of various magnitude and duration. Intrusive forces were generated by closed coil springs. Force magnitudes were 15-30gm, 50-60gm and 80-110gm. Durations were continuous and intermittent. Intermittent duration was applied at intervals of 12 hours. The readjustment of the force was done every 4 days. The forces were maintained for 30 days. All specimens were decalfied, embedded in paraffin and stained with hematoxylin-eosin stain. Observations were made with light microscope. The following results were obtained; 1. The continuity of root surface was ceased in all, except intermittent forced teeth with 25gm. 2. The root resorptions, cementoclasts and cemental lacunae, were increased around periapical regions and the destructive scope of bone was deeply extended as the exerted orthodontic forces increased. 3. It was inspected that, under the same forces, root resorption and bone destruction were more deeply appeared in the continuously forced teeth than the intermittently forced ones. 4. All of the alveolar bone, showed direct and undermining bone resorptions.

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A Study of Decorative Pattern Shown in e Mural Painting of Koguryo Dynasty′s Tomb (고구려고분벽화에 나타난 장식문양 연구)

  • 안창현
    • Journal of the Korean Society of Costume
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    • v.53 no.4
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    • pp.131-148
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    • 2003
  • Mural Painting of Koguryo Dynasty. root of Korean traditional pattern. has 5 types of pattern in its 24tombs: Fire pattern. Sun . Moon pattern, Flying angel pattern. Hill.Tree pattern, Holy animal bird Pattern. These patterns had developed specially in 3 regions that are Hwanghae-region, Pyongan-region, Jipan-region. from 4th-7th Century. A lot of fire pattern has found at Pyongan-region in 5th century. The ceiling right above 'Dori' frequently had the pattern. The pattern is organized in 'Kyoho' method. The sun.moon pattern was shown in Pyongan-region in 5th century. '3-leg bird'. representative of sun, and frog, representative of moon. were drawn in the pattern in 6th century, rabbit was added with frog in the pattern. Flying angel pattern had found most in Jipan region in 5th century. The pattern was not found in 4th century. Two types of the pattern are angels playing $$\mu$ical instruments and angels preying. The hill tree pattern was simple, antique, and immature in the beginning. the pattern had developed with real description as a landscape picture after the beginning. this pattern has been categorized as a landscape painting. Holy animal and bird patternn had placed in supporting rock between ceiling and floor with the mean of protection in after life These pattern, which were previously influenced by Chines culture, were developed with a base of Koguryo own tradition and supported establishment of a characterized Koguryo Cloture. This study will be a basic document for modern fashion industry of 21 century.