• 한국약용작물학회지
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• 제12권5호
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• pp.372-377
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• 2004

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

• Plant Resources
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• 제6권3호
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• pp.170-174
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• 2003

The important component for medical effect in ginseng is ginsenoside. Korea Ginseng & Tobacco Research Institute contains approximately 200 lines produced by inbred selection. It is assumed that ginseng lines containing high level of ginsenoside should be included in those lines. Besides, new breeding methods such as cell line selection in vitro and hairy root were recently developed. Therefore, this study was carried out to detect genes related to ginsenoside, and to use it for selection marker to select and distribute lines containing high level of ginsenoside. DNA was extracted from both ginseng roots and hairy roots, and the difference between the line containing high ginsenoside(KG101) and normal ginsenoside(KG103) were analysed. As a result, 28 out of 36 primers showed bands, and many primers showed band difference between ginseng lines. It is considered that the bands should be analysed using DNA sequence comparison to check if those are related to ginsenoside. In case of hairy roots of ginseng, almost no differences were found between two lines.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.294-300
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• 2009

Activation tagging that uses T-DNA vectors containing multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene is a powerful tool to determine gene function in plants. This approach has been successfully applied in screening various types of mutations and cloning the corresponding genes. We generated an activation tagged hairy root pool of ginseng (Panax ginseng C.A. Meyer) in an attempt to isolate genes involved in the biosynthetic pathway of ginsenoside (triterpene saponin), which is known as the major active ingredient of the root. Quantitative and qualitative variation of ginsenoside in activation tagged hairy root lines were profiled using LC/MS. Metabolic profiling data enabled selection of a specific hairy root line which accumulated ginsenoside at a higher level than other lines. The relative expression level of several genes of triterpene biosynthetic pathway in the selected hairy root line was determined by real time RT-PCR. Overall results suggest that the activation tagged ginseng hairy root system described in this study would be useful in isolating genes involved in a complex metabolic pathway from genetically intractable plant species by metabolic profiling.

• 한국산림과학회지
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• 제98권2호
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• pp.142-147
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• 2009

Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the 'Dragendorff's reagent' for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff's reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff's reagent is of great practical value in selection of invisible compounds.

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.173-179
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• 2003

The combination of a radiation technique with an in vitro culture system was appiled to develop submergence tolerant rice. The 3,000 $M_3$ lines with an average 80 percent of fertile grain were utilized for the selection of submergence tolerance. Salt tolerant lines were selected based on high plant height, root length and root number after submergence in plastic pots. Of the lines tested, the tolerant line (403-6) showed a dramatic difference in morphological traits under submergence compared to its original variety (Dongjinbyeo). It was suggested that genetic variations between the original variety and $M_3$-403-6 did exist. The levels of $\alpha$-amylase and alcohol dehydrogenase activities were significantly increased in the mutant line compared to its original variety. The mutant with greater tolerance showed less electrolyte leakage indicating a greater membrane integrity and better survival. Also, this line was much more resistant to a salt stress of $1.25\%$ than the original variety. The proline level of the line was significantly (p<0.01> higher than that of the original variety. The relationships between the inhibition of growth caused by stress and the physiological changes in the plant cell were discussed.

• 한국약용작물학회지
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• 제5권3호
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• pp.191-195
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• 1997

참당귀(當歸) 묘상기(苗床期)에서의 유묘특성(幼苗特性)과 추태율(抽苔率) 및 뿌리 수량(收量)과의 관계(關係)를 밝혀 유식물(幼植物) 시기(時期)에 조기검정(早期檢定)의 선발체계(選拔體系) 확립(確立)을 위한 기초자료(基礎資料)를 얻고자 시험을 실시한 결과는 다음과 같다. 1. 공시계통간(供試系統間) 묘초장(苗草長), 엽장(葉長), 엽병장(葉柄長) 등 지상부(地上部) 유묘특성(幼苗特性)에 유의적(有意的)인 차이가 나타나 묘상(苗床)에성의 조기검정(早期檢定) 가능성을 보였다. 2. 성숙기(成熟期)의 추태율(抽苔率)은 $0.6{\sim}75.6$% 범위였고, 뿌리수량(收量)은 $350{\sim}3,893kg/ha$으로 계통간(系統間) 차이가 컸으며, 추태율(抽苔率)이 5%미만으로 낮을 때 뿌리수량(收量)이 높았다. 3. 유전력(遺傳力)은 묘상(苗床)에서 엽병장(葉柄長), 묘초장(苗草長), 엽장(葉長) 등과 성숙시(成熟時)의 추태율(抽苔率), 뿌리수량(收量)등에서 92%이상으로 높았고, 엽폭(葉幅), 근경(根徑), 근장(根長) 등에서 낮게 나타났다. 4. 유묘특성(幼苗特性)과 추태율(抽苔率) 및 뿌리수량간(收量間)에는 관계(關係)는 묘초장(苗草長), 엽장(葉長), 엽폭(葉幅), 엽병장(葉柄長), 엽수(葉數), 근장(根長) 등은 뿌리수량(收量)과 부(負)의 상관(相關)을 보인 반면, 근경(根徑)은 정상관(正相關)을 나타내었다. 추태율(抽苔率)과 뿌리수량간(收量間)에는 부(負)의 상관(相關)으로 고도(高度)의 유의성(有意性)이 인정되었다. 5. 본 결과로 볼 때 묘상기(苗床期)에 묘초장(苗草長), 엽장(葉長), 엽병장(葉柄長) 등 유묘특성(幼苗特性)을 검정(檢定)하므로서 추태율(抽苔率)이 낮고 다수성(多收性)인 우량개체(優良個體)를 조기(早期)에 선발(選拔)할 수 있을 것으로 기대된다.

• 한국지능시스템학회논문지
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• 제9권4호
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• pp.384-388
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• 1999

This paper describes an approach for synthesizing a modularized gain scheduled PD type fuzzy logic controller(FLC) of nonlinear magnetic bearing system where the gains of FLC are on-line adapted according to the operating point. Specifically the systematic procedure via root locus technique is carried out for the selection of the gains of FLC. Simulation results demonstrate that the proposed gain scheduled fuzzy logic controller yields not only maximization of stability boundary but also better control performance than a single operating point (without gain scheduling)fuzzy controller.

• Plant Resources
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• 제6권1호
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• pp.1-6
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• 2003

Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22 $^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

• 한국자원식물학회지
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• 제10권2호
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• pp.135-139
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• 1997

식방풍 1호는 1990년에서 1995년까지 6개년간 전국에서 수집한 11개 식방풍재래종 중 영덕종 등 4계통을 순계분리한 후 생산력 검정시험에 공시한 다음 그 중 생육특성이 우수한 영덕종과 봉화종에 대해 지역적응시험 및 농가실증시험을 실시한 결과 영덕종이 다수성 순계임이 확인되어 1995년도에 농촌진흥청 우량종자선정 심의회에서 우량품종으로 결정되어 식방풍 1호로 명명되었기에 육성 과정에서 나타난 주유특성과 수량성을 요약하면 다음과 같다. 1. 식방풍 1호는 봉화식방풍과 초장은 비슷하고 주근장은 짧았으나 엽수가 많고 경자과 근두직경은 굵었고 종실량도 증수되었다. 2. 식방풍 1호는 근피색이 황갈색이고 근 형태는 식립원추형으로서 양질의 생약 재 생산에 유리한 품종이었다. 3. 식방풍 1호는 근중 엑스함량과 회분 및 산불용성 회분 함량으로 보아 대한 약전 규격집에 규정된 함량치에 적합하여 생약으로 이용시 유효성을 갖추었다. 4. 식방풍 1호는 토양선층에 비교적 강한 편이었고 내병성, 내습성, 내서성 및 내도복성도 강한 특성을 나타내었다. 5. 식방풍 1호의 건근수량은 생산력 검정시험, 지역적응시험 및 농가실증시험 결과 모두 봉화 식방풍보다 ha당 $10\sim28%$ 증수되었다.

• 한국약용작물학회지
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• 제8권3호
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• pp.283-289
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• 2000

내추대성 참당귀 노지육묘 이식재배시 묘두직경에 따른 생육 및 추대반응을 구명하고자 수행한 시험결과를 요약하면 다음과 같다. 출현율은 묘두직경이 클수록 높고 적올수록 낮았으며, 지상부 및 뿌리생육은 묘두직경이 클수록 양호하고 묘두직경이 적을수록 부진하였다. 추대시기는 묘두직경이 굵을수록 빨라지고 적을수록 늦어졌으며, $0.11{\sim}0.3,\;0.31{\sim}0.5,\;0.51{\sim}0.7,\;0.71{\sim}0.9,\;0.91{\sim}1.1cm$로 묘두직경이 굵어짐에 따라 각각 0.2%, 1.6%, 3.3%, 11.8%, 46.6%로 증가되었다. 10a당 수량은 $0.51{\sim}0.7cm$에서 319kg 으로 가장 높았으며, $0.51{\sim}0.7cm$보다 묘두직경이 작을수록 수량이 낮아지고 0.91cm 이상 묘에서는 추대율이 높아 수량이 낮았다. 추대율은 초장과 정(正)의 상관을 보였으며, 건근수량은 추대율과 부(負)의 상관을 보였다. 추대율이 비교적 낮고 수량이 가장 많은 묘두직경은 $0.51{\sim}0.7cm$였으며, 당귀재배시 적정 묘두직경의 범위는 $0.31{\sim}0.7cm$였다.