• Journal of Microbiology and Biotechnology
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• 제13권4호
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• pp.522-528
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• 2003

The growth responses of Phellodendron amurense root-derived materials against seven intestinal bacteria were examined, using an impregnated paper disk agar diffusion method and spectrometric method under $O_2$-free condition. The biologically active constituent of the P. amurense root extract was characterized as berberine chloride ($C_{20}H_{18}NO_{41}Cl$) using various spectroscopic analyses. The growth responses varied depending on the bacterial strain, chemicals, and dose tested. At 1 mg/disk, berberine chloride strongly inhibited the growth of Clostridium perfringens, and moderately inhibited the growth of Escherichia coli and Streptococcus mutans without any adverse effects on the growth of three lactic acid-bacteria (Bifidobacterium bifidum, B. longum, and Lactobacillus acidophilus). The structure-activity relationship revealed that berberine chloride exhibited more growth-inhibiting activity against C. perfringens, E. coli, and S. mutans than berberine iodide and berberine sulfate. These results, therefore, indicate that the growth-inhibiting activity of the three berberines was much more pronounced as chloridated analogue than iodided and sulphated analogues. As for the morphological effect caused by 1 mg/disk of berberine chloride, most strains of C. perfringens were damaged and killed, indicating that berberine chloride showed a strong inhibition against C. perfringens. As naturally occurring growth-inhibiting agents, the P. amurense root-derived materials described could be useful as a preventive agent against diseases caused by harmful intestinal bacteria such as clostridia.

• 한국식품저장유통학회지
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• 제24권6호
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• pp.778-785
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• 2017

본 연구는 시중 유통되고 있는 새싹채소 재배용 적콜라비 종자에서 분리한 E. coli strain RC-4-D의 생장예측모델을 개발하기 위해 수행되었다. 각 온도조건(10, 15, 20, 25, $30^{\circ}C$) 별로 적콜라비 중 E. coli strain RC-4-D 밀도 변화를 조사하였고 Baranyi model을 1차 생장예측모델로 이용하였고 각 온도별로 최대생장률(${\mu}max$)과 $10^{\circ}C$를 제외한 유도기(LPD) 값을 도출하였다. E. coli strain RC-4-D의 최대생장률에 대한 2차 생장예측모델로써 suboptimal Ratkowsky square-root, suboptimal Huang square-root, suboptimal Arrhenius-type 세 종류의 모델을 비교하였다. 모델 적합성 검정 결과, suboptimal Huang square-root 모델이 정확도가 가장 높고 suboptimal Ratkowsky square-root 모델이 편차가 가장 적은 것으로 나타났다. 종합적으로, RMSE가 0.100, $A_f$가 1.255, $B_f$가 0.999인 suboptimal Ratkowsky square-root 모델이 온도의 영향을 설명하는 가장 적합한 2차 생장예측 모델인 것으로 나타났다. 본 연구에서 개발한 모델은 적콜라비 새싹채소 생산에 있어서 E. coli의 생장을 예측하고 미생물 위해성평가를 수행하는데 활용될 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1777-1789
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• 2019

Drought is more concerned to be a huge problem for agriculture as it affects plant growth and yield. Endophytic bacteria act as plant growth promoting bacteria that have roles for improving plant growth under stress conditions. The properties of four strains of endophytic bacteria were determined under water deficit medium with 20% polyethylene glycol. Bacillus aquimaris strain 3.13 showed high 1-aminocyclopropane-1-carboxylate (ACC) deaminase production; Micrococcus luteus strain 4.43 produced indole acetic acid (IAA). Exopolysaccharide production was high in Bacillus methylotrophicus strain 5.18 while Bacillus sp. strain 5.2 did not show major properties for drought response. Inoculation of endophytic bacteria into plants, strain 3.13 and 4.43 increased height, shoot and root weight, root length, root diameter, root volume, root area and root surface of Jerusalem artichoke grown under water limitation, clearly shown in water supply at 1/3 of available water. These increases were caused by bacteria ACC deaminase and IAA production; moreover, strain 4.43 boosted leaf area and chlorophyll levels, leading to increased photosynthesis under drought at 60 days of planting. The harvest index was high in the treatment with strain 4.43 and 3.13 under 1/3 of available water, promoting tuber numbers and tuber weight. Inulin content was unchanged in the control between well-watered and drought conditions. In comparison, inulin levels were higher in the endophytic bacteria treatment under both conditions, although yields dipped under drought. Thus, the endophytic bacteria promoted in plant growth and yield under drought; they had outstanding function in the enhancement of inulin content under well-watered condition.

• 한국작물학회지
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• 제49권4호
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• pp.284-288
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• 2004

The response of dissolved oxygen (DO) concentrations caused significant change in root number, root length, coleoptile length, shoot length and leaf age of seedlings. The genotypic difference in the effect of DO also highly significant (P<0.01) for all of the seedling traits. The number and length of root were extremely inhibited at the condition of $0.39\pm0.09$ DO concentration. While the coleoptile elongated markedly in the lowest DO concentrations, the shoot did not develop. The root growth was improved slightly at the $1.39\pm0.27mg L^{-1}$, however, there were no difference among genotypes at these two low DO concentrations. As the DO concentration become higher, the growth of root and shoot was improved remarkably. Root number, root length and shoot length was significantly different between $20\;and\;30^{\circ}C$ in DO rich and normal conditions, the development of those traits were apparently accelerated in high water temperature, however those traits of seedlings in DO deficiency were not different between the two temperatures except for shoot length. On the other hand the coleoptile length was not affected by the stagnant water temperature; it was stimulated by the low DO concentration. The competition of DO was greater as the seedling density was increased in the stagnant water, therefore the seedlings grown under high density have long and white coleoptiles, and the growth of roots and shoots was retarded severely.

• 한국환경복원기술학회지
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• 제16권3호
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• pp.97-105
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• 2013

Kalopanax septemlobus (Thunb. ex Murray) Koidz. is natively distributed in Korea. The importance of this species has been increased not only for high quality timber but for medicinal and edible uses. However, increasing market demand of K. septemlobus with illegal cutting and overexploitation has resulted in its rapid depletion and destruction of natural habitat. This study was conducted to understand the survival rate and growth characteristics of planted K. septemlobus seedlings with treatment of root pruning, stem cutting and planting density. The survival rate and growth of height and root-collar diameter for one- and two-year old seedlings with different planting densities were investigated in the clear-cut area of a Pinus densiflora stand for five years. One-year-old seedlings were treated with or without root pruning and planted with three density levels (5,000 trees $ha^{-1}$, 10,000 trees $ha^{-1}$, and 40,000 trees $ha^{-1}$). Two-year-old seedlings were treated with and without stem cutting and planted with the density of 5,000 trees $ha^{-1}$. The survival rate of one-year-old seedlings with root pruning treatment in the density of 10,000 trees $ha^{-1}$ was 92%, while that without root pruning in the density of 40,000 trees $ha^{-1}$ was 67% after five years. The height of one-year-old seedlings has been significantly affected only by planting density in the $5^{th}$ year. The survival rate of the two-year-old seedlings with stem cutting was 75.5% and greater than control (67.3%) in the $5^{th}$ year but no difference in height was shown between the two treatments from three years after plantation.

• 한국환경농학회지
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• 제39권3호
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• pp.204-213
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• 2020

BACKGROUND: Since the number of crops cultivated in reclaimed land is huge, it is very difficult to quantify the total crop production. Therefore, a non-destructive method for predicting crop production is needed. Salt tolerant root vegetables such as red beets and sugar beet are suitable for cultivation in reclaimed land. If their underground biomass can be predicted, it helps to estimate crop productivity. Objectives of this study are to investigate maximum leaf length and weight of red beet, sugar beet, and turnips grown in reclaimed land, and to determine optimal model with regression analysis for linear and allometric growth models. METHODS AND RESULTS: Maximum leaf length, width, and root fresh weight of red beets, sugar beets, and turnips were measured. Ten linear models and six allometric growth models were selected for estimation of root fresh weight and non-linear regression analysis was conducted. The allometric growth model, which have a variable multiplied by square of maximum leaf length and maximum leaf width, showed highest R² values of 0.67, 0.70, and 0.49 for red beets, sugar beets, and turnips, respectively. Validation results of the models for red beets and sugar beets showed the R² values of 0.63 and 0.65, respectively. However, the model for turnips showed the R² value of 0.48. The allometric growth model was suitable for estimating the root fresh weight of red beets and sugar beets, but the accuracy for turnips was relatively low. CONCLUSION: The regression models established in this study may be useful to estimate the total production of root vegetables cultivated in reclaimed land, and it will be used as a non-destructive method for prediction of crop information.

• 생물환경조절학회지
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• 제32권3호
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• pp.205-209
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• 2023

기계적 자극은 식물체의 형태적 변화를 유도하는 것으로 알려져 있다. 본 연구에서는 기계적 자극을 적용한 토마토의 육묘기 동안의 생육 변화를 확인하기 위해 수행되었다. 브러싱 처리가 기계적 자극으로 이용되었으며, 아크릴이 부착된 이송장치를 이용하여 2시간 간격으로 파종 후 10일 이후부터 처리를 진행하였다. 브러싱 처리 동안 생육 변화를 확인하기 위해 3일 간격으로 생육을 조사하였다. 초장과 엽면적은 브러싱 처리에서 대조구에 비해 감소하는 결과를 보였으며 지상부의 생체중과 건물중은 대조구와 유사한 값을 보였다. 총 근장과 지하부 표면적은 브러싱 처리에서 대조구에 비해 증가하였으며, 지하부 부피는 대조구와 브러싱 처리가 유사한 값을 보였다. 결론적으로 토마토 공정묘에 브러싱 처리는 지상부의 생육 감소를 유도하였으며 지하부의 형태적 발달을 증가시키는 것으로 판단된다.

• 생명과학회지
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• 제22권5호
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• pp.681-686
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• 2012

빛이 굴중성과 뿌리 생장에 미치는 영향을 알아보기 위해 Arabidopsis phytochrome mutant를 이용하여 뿌리 생장과 굴중성 반응을 측정하였다. $PhyA$의 뿌리 생장은 white light를 제외한 모든 빛 조건에서 WT와 비교하여 촉진되었다. 특히 red light에서 키웠을 때 다른 mutant와는 달리 뿌리 생장이 촉진되었다. 반면에 phyB는 모든 빛 조건에서 키웠을 때 뿌리 생장이 억제되었으며, 특히 double mutant인 $phyAB$는 white light와 red light에서 키웠을 때 뿌리 생장이 가장 많이 억제되었다. $PhyA$의 굴중성 반응은 far-red light에서 키웠을 때를 제외하고 모든 빛 조건에서 키웠을 때 WT와 비교하여 촉진되었다. 한편 $phyAB$는 모든 조건에서 모두 굴중성 반응이 억제되었다. Ethylene 생합성을 조절하는 효소인 ACS transcript 수준은 white light와 red light에서 키웠을 때 $phyA$가 높게 나타났으며, far-red light에서 키웠을 때는 $phyA$의 transcript 수준이 억제되었다. 결론적으로 뿌리 생장과 굴중성 반응은 $phyB$의 $P_{fr}$에 의해 조절된다.

• 한국약용작물학회지
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• 제10권4호
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• pp.243-248
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• 2002

The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.

• 한국조경학회지
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• 제31권6호
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• pp.77-84
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• 2004

This study was carried out to analyze the vertical and horizontal distribution of fine root biomass of Pinus thunbergii transplanted in reclaimed land from the sea near Gwangyang Bay. The fine-root biomass according to 6 planting ground types were as follows: 98.5 g DM/$m^2$ for P2, 51.1 g DM/$m^2$ for P6, 47.8 g DM$m^2$ for P5, 44.6 g DM/$m^2$ for P3, 38.2 g DM/$m^2$ for P4, 31.8 g DM/$m^2$ for Pl, respectively. The vertical distribution of fine root biomass decreased at descending soil depths of the 6 mounding types. Fine root biomass was 31∼55% in the topsoil of 20cm depth. Fine root biomass that were related to the Spatial distance from the nearest tree were unevenly distributed horizontally in 6 stands. distribution patterns of fine root biomass were closely related to soil hardness and alkalic cation (Ca++, Mg++, Na+, K+) concentrations. Therefore, in order to have good condition for the growth of landscaping plants, we suggest that there is a need for the construction of planting grounds as well as a need for soil improvement in bad soil environments.