The two-month-old rooted-cuttings of Populus alba ${\times}$ glandulosa, P. euramericana and P. nigra ${\times}$ maximowiczii clones were exposed to livestock waste water - one of major water pollutants, and ground water in order to determine the effects of livestock waste water on growth response and absorption capacity of the species. For this purpose, 5 clones of each species were used. In all the species, the height growth of rooted-cuttings was better in livestock waste water treatment than in ground water. Of all the poplar species compared, the height growth was best in P. alba ${\times}$ glandulosa. In the cases of P. euramericana and P. nigra ${\times}$ maximowiczii, the height growth in the livestock waste water treatment was statistically different among clones, whereas there was no significant difference among P. alba ${\times}$ glandulosa clones. Aboveground biomass such as leaf and shoot dry weight of all the species increased in the livestock waste water treatment, while root dry weight decreased. In addition, chlorophyll contents in leaf of all the poplar species increased in the livestock waste water treatment. All the poplar trees showed temporal variation in the absorption amount of livestock waste water during the experimental period. In all the poplar species, the absorption amount of livestock waste water was less than that of ground water. Of 3 poplar species, P. alba ${\times}$ glandulosa was best in the absorption capacity of livestock waste water. Of all 15 poplar clones compared in this study, the 72-16 clone of P. alba ${\times}$ glandulosa showed the best absorption capacity.
This experiment was carried out to examine the effect of a silicate fertilizer on the growth and development of potted plants. Cutting of Kalanchoe blossfeldiana 'Kaluna' and 'Taos', and Dianthus caryophyllus L. 'Kazan' and 'Tula' were grown in 50 and 128-cell plug trays, respectively. Rooted cuttings transplanted to the mixture of a commercial medium and perlite (5:1, v/v) supplemented with a silicate fertilizer at 0, 40, 80, 120 or $160g{\cdot}L^{-1}$ medium was evaluated. A silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in the greatest plant height, leaf thickness, and root fresh and dry weights in both kalanchoe and carnation. However, plant height was suppressed in the treatment of a silicate fertilizer supplementation at higher concentrations in both kalanchoe and carnation. According to the scanning electron microscope images of transversal sections of tissues of roots and leaves in kalanchoe and carnation, the treatment of a silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in plants with more compact tissue than the control.
Cheon, Mi Geon;Lee, Seo Hyoun;Park, Kyung Mi;Choi, Seong-Tae;Hwang, Yeon Hyeon;Chang, Young Ho;Kim, Jin Gook
Journal of Bio-Environment Control
/
v.30
no.3
/
pp.244-249
/
2021
The hydroponic culture for growing 'Duke' blueberry was evaluated in a protective greenhouse provided with similar environmental conditions to the conventional blueberry cultivations. One year old 'Duke' blueberry bushes planted in 180 L containers filled with 130 L peat moss and 40 L pearlite (v/v) were selected for the experiment. A nutrient solution consisted with NO3-N 4.6, NH4-N 3.4, PO4-P 3.3, K 3, Ca 4.6, and Mg 2.2 mmol-1 was supplied to the plants, comparing non-hydroponic treatment (provided with only underground water). Hydroponic culture increased number of shoot per bush by 18% and total shoot length by 24% compared with non-hydroponic culture. Total dry weight of a bush increased in the hydroponic with vigorous root growth 1.4-fold more than the non-hydroponic. Higher concentrations of inorganic elements and organic compounds were found in the hydroponic, indicating active nutrient absorption of the bush. The hydroponic produced high yield similar to adult bush from 4 years old age, maintaining the yield until 8 years old age. The findings of this study indicated that hydroponic cultivation systems will be useful for growing blueberry crop.
The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$$m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$$m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$$m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$$m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$$d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.
The objectives of this study were to understand the physiological responses and resistance of red pine trees to ozone exposure in relation to nitrogen and phosphorus fertilization. Potted one-year-old seedlings of Pinus densiflora S. et Z. were exposed in an open-top chamber(OTC) to ozone at concentration of 0.12ppm for 3 hours daily for eight weeks with or without N and P fertilization alone or in combination. The OTC had dimensions of 2.0m in height and 2.5m in diameter, and the air in a control chamber was filtered with activated charcoal to maintain the ozone concentration below 0.02ppm. After eight weeks of ozone exposure, none of the seedlings showed any symptoms of visible injury on leaves. The seedlings fertilized with N and P in a control chamber showed 22 to 95% increase in total dry weight, and similar fertilizer effect was also noticed in an ozone chamber. Ozone treatment did not decrease the total dry weight, but increased shoot/root ratio by 14.5%. Ozone treatment increased sucrose content in the leaves by 23%, but decreased sucrose content in roots by 20% regardless of N or P application. Starch content in the leaves was not affected by either ozone or fertilizer. However, starch content in the roots was decreased by 41% by ozone treatment. Chlorophyll content in the leaves was increased by 70% by N application, but was not affected by ozone treatment. Nitrogen and P fertilization stimulated net photosynthesis by 80% in a control chamber, but stimulatory effect of N and P on net photosynthesis was 22.3% less in an ozone chamber. Net photosynthesis of the seedlings with no fertilization was not affected by ozone treatment. Based on the observed interactions between N, P, and ozone, it was concluded that the stimulatory effect of fertilization on growth of Pinus densiflora would be decreased by ozone treatment, but fertilization would increase resistance to ozone by re-allocation of increased carbohydrates.
Yoon, Jun Hyuck;Jeon, Kwon Seok;Song, Ki Seon;Park, Yong Bae;Moon, Yong Sun;Lee, Do Hyung
Journal of Korean Society of Forest Science
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v.103
no.1
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pp.65-71
/
2014
Cacalia firma is a perennial plant in Asteraceae, Parasenecio that distributed in Korea, China, and Japan. As dietary style changes for well-being life, consumer's demand of functional food and organic vegetables is getting increased. This study was conducted to investigate the optimum light conditions of P. firmus in forest farming. One year old seedlings were grown under four different light conditions 10%, 20%, 30%, and 50% of sunlight by shading (equals 50%, 30%, 20%, and 10% relative brightness respectively) and non-treated control under full sunlight. They were analyzed for early growth and physiological response. Seedlings grown under 75% shading showed similar height, root growth, and leaf water content to control. However, their leaf length, width, and total leaf area were increased, which caused increased leaf dry weight and total dry weight. Especially, seedlings under 95% shading showed 40% increase in height and more leaf growth and leaf water content, although they had shorter main root length and root collar diameter than control. In addition specific leaf area (SLA) and leaf area ratio (LAR) were higher than control and indicated that they were statistically significant difference from control. Higher SLA refers thinner leaf thickness, higher LAR means larger leaf area. The results indicate seedlings under 95% shading have higher water content, thinner leaf, and wider lightinterception areas. It is plausible that P. firmus is active in chlorophyll activities and carbon dioxide assimilation at even lower light conditions. These results suggest that the optimum light level of P. firmus for artificial cultivation in forest farming ranges from 75~95% shading (20%-10% of relative brightness). When salability as 'sanchae' (wild edible greens) is considered, P. firmus could be cultivated under 75% shading in forest farming and expected to have better taste and higher yield. We suggest these results as basic data of P. firmus for possible forest farming.
This study was carried out to investigate classfication of weedy rice (Oryza sativa) based on isozymes esterase and peroxidase, growth and developmental difference of weedy rices and rices grown under dry and water condition, and weedy rice control and tolerant difference of weedy rices in various herbicides using weedy rices collected from thirteen strains of Chonnam, one Chonbuk, two Kyeongki and two rice cultivars. 1. The collected weedy rices were classified into three groups based on isozyme esterase and peroxidase using polyacrylamide gel electrophoresis(PAGE) method. The classified groups were not same each other. 2. Plant height was taller in collected weedy rices than rice cultivars at 18 days after seeding under dry and water conditions, but number of leaves, shoot fresh weight, root fresh weight and root length were not significantly different between collected weedy rices and rice cultivars. In addition, growths of collected weedy rices were greater in dry- than water-condition. 3. After thiobencarb(S-4-chlorobenzyl diethythiocarbamate), molinate(S-ethyl hexahydro-1H-azepine-1-carbothioate) and oxadiazon(5-tert-butyl-3(2,4-dichloro-5-isopropoxyphenyl)-1,3,4-oxadiazol-2-one) were applied at 6 days before seeding, the weedy rices controlled 100% by thiobencarb at 2.1kg ai/ha and 024kg ai/ha oxadiazon treatment but controlled 26% to 67% by molinate at 6.5kg ai/ha. Rice due to the herbicides was injured severely(25% to 100%) in flood condition at time of rice seeding after oxadiazon at 0.48kg ai/ha and 2.1kg ai/ha thiobencarb application, except for molinate which injured rice slightly(4% to 13%) in drain condition. The collected weedy rices to all experimented herbicides showed slight intraspecific variations. The intraspecific variations of weedy rices decreased in the order of thiobencarb>molinate>oxadiazon.
This study was carried out to evaluate the growth characteristics, carbon and nitrogen content of containerized 1-0 Chamaecyparis obtusa seedlings at various levels of fertilizer (2 g/L, 1 g/L, control) and three container volumes (500 mL, 400 mL, 320 mL). The growth of root collar diameter was highest in the 2 g/L (3.14 mm), followed by the 1 g/L (2.75 mm) and control (2.41 mm) treatments, while the height of seedling was significantly higher in the 1 g/L (21.88 cm) than other treatments (2 g/L: 20.92 cm; control: 19.06 cm). The growth of root collar diameter by container volume was better in the 500 mL than in the 320 mL. Dry weight of seedling was the highest in the 1 g/L ($4.41g\;seedling^{-1}$), followed by the control ($3.67g\;seedling^{-1}$) and the 2 g/L ($2.92g\;seedling^{-1}$) treatments. The dry weight of seedlings by container volume was significantly higher in the 500 mL than in the 320 mL. Nitrogen concentration in foliage was ranged from 1.51% in the control to 2.45-2.60% in the fertilizer treatments. However, carbon concentration of seedlings was not affected by the fertilizer or the container volume treatments. The growth of seedlings following planting in mountain area was better in the fertilized seedlings compared with in the unfertilized seedlings. The results indicate that the 1 g/L fertilization was an optimum rate for growth following planting of Chamaecyparis obtusa seedlings.
Fifty-day-old plug seedlings of yacon (Polymnia sonchiflolia Poeppig & Endlicher) were transplanted on Mar. 23, Apr,8, Apr,23, May 8, May 23, and June 7,2002 at Jeju to evaluate the effect of transplanting date on the growth and yield of yacon in Jeju island. Delaying the transplanting until May 23 greatly decreased plant height stem height, main stem diameter and number of nodes per plant. The number of marketable tuberous roots was about 6.0 per plant with the early transplanting from March 23 to April 231 but decreased to 1.9 per plant with the latest transplanting time, June 7. The yield of marketable tuberous roots of early transplanted (March 23 to April 23) yacon was about 27 MT/ha, and was 32.4 MT/ha at April 23 transplanting, but was remarkably reduced at the three later transplantings, May 8, May 23, and June 7. Dry matter and soluble solid contents of marketable tuberous roots were 14% and 15%, respectively, regardless of transplanting date. The weight of a marketable tuberous root ranged from 153 to 183.9 g at the first three transplantings, but gyeatly reduced with delaying transplanting until May 7. The optimum transplanting date for yacon in Jeju island seems to be from mid-April to late April considering tuberous root yields, the risk of late-spring frost, and cost of seedling raising.
Lee, Sung Woo;Lee, Seung Ho;Seo, Moon Won;Jang, In Bok;Jang, In Bae;Yu, Jin;Moon, Ji Won;Suh, Soo Jung
Korean Journal of Medicinal Crop Science
/
v.26
no.3
/
pp.248-253
/
2018
Background: Dazomet are widely used as soil fumigant to solve soilborne problems, and the degradation intermediates are toxic to nematodes, fungi, bacteria, insects and weeds. Methods and Results: The effects of cultivation of green manure crop, maize before and after soil fumigation on the control of ginseng root rot disease were compared using soil where 6-years-old ginseng was harvested. Fumigant (dazomet) were used for soil fumigation in May and September, respectively. Maize was grown for soil management before and after soil fumigation. After May fumigation, the sowing date of maize was delayed by 15 days and thus its dry weight was decreased significantly. Maize cultivation after May fumigation increased pH but decreased EC, $NO_3$, $P_2O_5$, and K significantly. Maize cultivation after May fumigation decreased fungi population and the ratio of fungi and bacteria. Growth of 2-years-old ginseng was improved and the incidence of ginseng root rot was significantly decreased by maize cultivation after May fumigation. After harvesting 2-years-old ginseng, the population of Cylindrocarpon destructans was not different between treatment of May and September, but Fusarium solani showed a significant increase in September fumigation after maize cultivation. Conclusions: Maize cultivation after soil fumigation was effective in inhibiting ginseng root rot by the amendment of mineral composition and microorganism in fumigated soil.
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