• Journal of Ginseng Research
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• 제26권3호
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• pp.151-158
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• 2002

Ginseng (Panax quinquefolius L.) has become one of the most valuable herb crops grown in North America. However, traditional cropping practices are favourable to disease and significant losses due to root disease are common, despite frequent use of fungicides. Seedlots are often contaminated with pathogens, however, little is known about the causes of seed decay and the role of seed pathogens as incitants of root rots. It was shown that both Fusarium spp. and Cylindrocarpon destructans were able to rot seeds and that C. destructans was more virulent than Fusarium spp. on seedling roots. A modified rose bengal agar MRBA) medium (1 g KH$_2$PO$_4$; 0.5 g MgSO$_4$; 50 mg rose bengal; 10 g dextrose; 5 g Bacto peptone; 15 g Bacto agar; 30 mg streptomycin sulfate; 250 mg ampicillin; 10 mg rifampicin; 500mg pentachloronitrobenzene; 500 mg dicloran; and 1 L distilled water) was superior to potato dextrose agar in detecting C. destuctans in diseased roots. Isolation of C. destructans from diseased seedlings arising from seeds sown in replant soil supported the hypothesis that this pathogen is a cause of ginseng replant failure in North America.

• 한국균학회지
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• 제48권4호
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• pp.499-503
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• 2020

Clubroot symptoms were frequently observed on the roots of Baecheongchae plants grown in vinyl greenhouses of a farmer located in Yangpyeong area of Korea during a disease survey in June 2019. The incidence of diseased Baecheongchae plants ranged from 30 to 90% in the vinyl greenhouses investigated. Many resting spores were found in the tissue of root galls collected. The resting spores were hyaline and spherical and measured 2.5-4.2 ㎛ in diameter. Three inoculum suspensions of resting spores prepared from the root galls were inoculated to the roots of healthy Baecheongchae plants. All the inoculum suspensions caused clubroot symptoms to appear on the roots of the inoculated Baecheongchae plants. The symptoms on the roots induced by artificial inoculation were similar to those observed in the plants of the vinyl greenhouses during the disease survey. Resting spores of the pathogen were recovered from the root galls of the inoculated plants. Three root gall isolates obtained from the inoculated plants were used for molecular identification. Comparing the isolates to the Plasmodiophora brassicae strains in GenBank, the amplification products demonstrated 100% similarity with the internal transcribed spacer (ITS2) sequences. The clubroot pathogen was identified as P. brassicae according to its morphological, pathological, and molecular characteristics. This is the first report of P. brassicae causing clubroot in Baecheongchae.

• 식물병연구
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• 제13권2호
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• pp.104-109
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• 2007

양파 연작지 토양의 담수기간에 따른 분홍색뿌리썩음병의 방제효과, 양파의 생육 및 수량을 조사한 결과 토양병원균의 밀도변화는 담수기간이 길수록 균밀도의 감소효과가 뚜렷하여 처리 후 90일에는 무담수에 비해 $1/2{\sim}1/3$ 수준으로 감소하였지만 양파 재배후 균밀도는 담수직후 보다는 전반적으로 증가하는 경향을 보였다. 양파 육묘상 담수처리에 의한 분홍색뿌리썩음병의 방제효과는 담수 60일 이상 처리에서 $93.5{\sim}99.2%$였고, 뿌리이병율도 무담수에 비해 $1/11{\sim}1/18$ 수준으로 크게 감소하였다. 양파 재배포장에서의 발병율 또한 담수 60일 이상 처리에서 5% 미만으로 크게 감소하였다. 양파의 구중과 상품수량은 무담수에 비해 담수처리에서 증가되었으며, 담수기간이 길어질수록 대구의 비율이 늘어나는 경향을 보였다. 이와 같이 양파 분홍색뿌리썩음병이 심하게 발생하고 있는 연작지에 양파를 정식하기 전에 담수처리를 할 경우, 적어도 2년간, 60일 이상 담수처리를 하였을 때 효과적으로 분홍색뿌리썩음병균의 밀도와 병발생을 경감시킬 수 있으며, 정상적인 양파 육묘 및 생육에 의한 구비대로 수량 증수효과가 클 것으로 판단된다.

• Journal of Periodontal and Implant Science
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• 제24권1호
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• pp.165-177
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• 1994

To investigate the depth of the root concavity and root surface area of the maxillary first premolar, 40 maxillary first premolars were used. All the teeth which extracted because of advanced periodontal disease and orthodontic treatment procedure, were sectioned every 1.5mm from cementoenameljunction to the apex with hard tissue microtome. Each sectioned root was taken photograph with slide film, and projected for measuring with a calibrated digital Curvi-Meter. The root surface area, percentage of the RSA and the linear variation of the RSA were calibrated for each 1.5mm section. Linear variation of the depth of root concavity was measured on mesial and distal root surface for each section using computer-aided digitizer. The results were as follows. 1. The total mean root length of maxillary first premolar was 13.48mm. Mean buccal root length of 2-rooted tooth was 12.59mm, mean palatal root length was 12.73mm, and mean root length of single rooted tooth was 13.78mm. 2. The total mean root surface area of maxillary first premolar was $194.17mm^2$, mean root surface area for 2-rooted tooth was $205.97mm^2$ and mean root surface area for single rooted tooth was $188.49mm^2$. 3. It was 59.93% of the total root surface area that the area from CEJ to coronal 6mm. And, the coronal half of the root length accounted for approximately 71.76% of the total root surface area. 4. Most deepest concavity of the mesial root surface was 0.65mm at apical 3.0mm, 4.5mm level in maxillary first premolar. And, that of the distal root surface was 0.37mm at apical 4.5mm level. 5. All of the maxillary first premolar had mesial root surface concavity. This mesial root surface concavity appeared to be more pronounced in 2-rooted tooth than single rooted tooth.

• The Plant Pathology Journal
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• 제22권1호
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• pp.103-106
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• 2006

In this biocontrol research, we evaluated disease suppressive effects of antagonistic bacterial strains ISE13 and KJ1R5 against Korean ginseng root rot caused by P. eaetorum. We also examined the effects of nutrient solution in the hydroponic culture system for Korean ginseng on biological activity of the bacterial strains. As results of dual culture tests of the bacterial strains on $V_{8}$ juice agar, the strain ISE13 showed antifungal activity against P. eaetorum and other plant pathogenic fungi, but the strain KJ1R5 did not. When their inhibitory effects against infection of P. eaetorum on the roots grown in either nutrient solution or water were tested, the strains ISE13 and KJ1R5 inhibited the disease severity of Korean ginseng roots only grown with water, compared to buffer-treated, inoculated controls. However, the nutrient solution used for hydroponic cultures of ginseng in pots caused higher levels of disease severity by the strains ISE13 and KJ1R5 from 418.8\%$ to 40.0\%$ and from 24.3\%$ to 45.0\%$, respectively. In this study, the bacterial strains ISE13 and KJ1R5 could be potentially biocontrol agents to suppress Korean ginseng root rot caused by P. eaetorum. However, more attention using nutrient solution in hydroponic cultures for Korean ginseng production should be applied in biocontrol of plant diseases using the antagonistic microorganisms.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.65.2-65
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• 2003

To protect the plant against several soil-borne pathogens, we are currently constructing disease-resistant transgenic root stock for the growth of cucurbitaceae vegetable plants, watermelon and gourd. We made a watermelon cDNA library from Cladosporium cucumerinum-Infected leaves for substractive hybriazation and differential screening. We isolated the several pathogen inducible cDNA clones, such as caffeoyl-CoA-methyltransferase, LAA induced protein, receptor-like kinase homolog, hydroxyproline-rich glycoprotein, catalase, calmodulin binding protein, mitochondrial ATPase beta subunit, methyl tRNA synthetase and WRKY transcription factors. We previously obtained CaMADS in pepper and galactinol synthase ( CsGolS) in cucumber that were confirmed to be related with disease-resistance. CaMADS and CsGolS2 were transformed into the inbred line 'GO701-2' gourd, the inbred line '6-2-2' watermelon and the Kong-dye watermelon by Agrobacterium tumerfaciens LBA4404. Plant growth regulators (zeatin, BAP and IAA) were used for shoot regeneration and root induction for optimal condition. Putative transgenic plants were selected in medium containing 100mg/L kanamycin and integration of the CaMADS and CsGO/S2 into the genomic DNA were demonstrated by the PCR analysis. We isolated major soil-borne pathogens, such as Monosporascus cannonballus, Didymella bryoniae, Cladosporium cuvumerinum from the cultivation area of watermelon or root stock, and successfully established artificial inoculation method for each pathogen. This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Republic of Korea.

• The Plant Pathology Journal
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• 제19권3호
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• pp.181-183
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• 2003

Corky root symptoms caused by Pyrenochaeta lycopersici were observed on the roots and stem base of tomato plants in Korea. Symptoms on infected plants typically appeared as stunting and generally lacking vigor, and infected plants die back from the foliage tips after fruits have set. Brown lesions appearing with bands around the roots were characteristic symptoms of the disease. The lesions become swollen and cracked along the length of the root with corky appearance. Based on cultural and morphological characteristics, the fungus from the diseased plants was identified as Pyrenochaeta lycopersici. Pycnidia were solitary, globose to subglobose, brown to black, darker around the neck region, and measured 173-215 $\mu\textrm{m}$ in diameter with septate setae up to 102-132$\times$6.5 $\mu\textrm{m}$. Conidia were hyaline, unicellular, and 4.2-4.7$\times$l.5-2.0 $\mu\textrm{m}$ long. Optimum temperature for mycelial growth of the p. lycopersici isolates ranged from $20^{\circ}C$ to $25^{\circ}C$. Fifteen isolates off lycopersici were tested for pathogenicity to susceptible and tolerant cultivars of tomato plants by artificial inoculation. Three isolates of P. lycopersici induced typical corky root discoloration on susceptible tomato cultivars but not on tolerant tomato. This is the Erst report in Korea of tomato corky root disease caused by P. lycopersici.

• 한국산업보건학회지
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• 제32권4호
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• pp.425-434
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• 2022

Objectives: This study analyzes TVOC concentrations in root industry workplaces in order to prevent probable occupational disease among workers. Root industry includes all the infrastructure of manufacturing, such as casting and molding. Methods: Real-time TVOC sensors were deployed in three root industry workplaces. We measured TVOC concentrations with these sensors and analyzed the results using a data-analysis tool developed with Python 3.9. Results: During the study period, the mean of the TVOC concentrations remained in an acceptable range, 0.30, 2.15, and 1.63 ppm across three workplaces. However, TVOC concentrations increased significantly at specific times, with respective maximum values of 4.98, 28.35, and 26.65 ppm for the three workplaces. Moreover, the analysis of hourly TVOC concentrations showed that during working hours or night shifts TVOC concentrations increased significantly to higher than twice the daily mean values. These results were scrutinized through classical decomposition results and autocorrelation indices, where seasonal graphs of the corresponding classical decomposition results showed that TVOC concentrations increased at a specific time. Trend graphs showed that TVOC concentrations vary by day. Conclusions: Deploying a real-time TVOC sensor should be considered to reflect irregularly high TVOC concentrations in workplaces in the root industry. It is expected that the real-time TVOC sensor with the presented data analysis methodology can eradicate probable occupational diseases caused by detrimental gases.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1299-1308
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• 2023

Carya cathayensis is an important economic nut tree that is endemic to eastern China. As such, outbreaks of root rot disease in C. cathayensis result in reduced yields and serious economic losses. Moreover, while soil bacterial communities play a crucial role in plant health and are associated with plant disease outbreaks, their diversity and composition in C. cathayensis are not clearly understood. In this study, Proteobacteria, Acidobacteria, and Actinobacteria were found to be the most dominant bacterial communities (accounting for approximately 80.32% of the total) in the root tissue, rhizosphere soil, and bulk soil of healthy C. cathayensis specimens. Further analysis revealed the abundance of genera belonging to Proteobacteria, namely, Acidibacter, Bradyrhizobium, Paraburkholderia, Sphaerotilus, and Steroidobacter, was higher in the root tissues of healthy C. cathayensis specimens than in those of diseased and dead trees. In addition, the abundance of four genera belonging to Actinobacteria, namely, Actinoallomurus, Actinomadura, Actinocrinis, and Gaiella, was significantly higher in the root tissues of healthy C. cathayensis specimens than in those of diseased and dead trees. Altogether, these results suggest that disruption in the balance of these bacterial communities may be associated with the development of root rot in C. cathayensis, and further, our study provides theoretical guidance for the isolation and control of pathogens and diseases related to this important tree species.

• 한국약용작물학회지
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• 제26권5호
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• pp.345-353
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• 2018

Background: Some phenolics detected in the soil may inhibit the seed germination and seedling growth of ginseng (Panax ginseng). This study investigated the effect of irrigation and ginseng root residue addition on the soil microbial community and root rot disease in 2-year-old ginseng. Methods and Results: Each $20{\ell}$ pot was filled with soil infected with ginseng root rot pathogens, and irrigated daily with $2{\ell}$ of water for one month. After the irrigation treatment, ginseng fine root powder was mixed with the irrigated soil at a rate of 20 g per pot. In descending order, ${NO_3}^-$, electric conductivity (EC), exchangeable Na (Ex. Na) and K (Ex. K) decreased due to irrigation. In descending order, ${NO_3}^-$, EC, Ex. K, and available $P_2O_5$ increased with the additon of ginseng powder to the soil. The abundance of Trichoderma crassum decreased with irrigation, but increased again with the incorporation of ginseng powder. The abundance of Haematonectria haematococca increased with irrigation, but decreased with the incorporation of ginseng powder. The abundance of Cylindrocarpon spp. and Fusarium spp., which cause ginseng root rot, increased with the incorporation of ginseng powder. The abundance of Arthrobacter oryzae and Streptomyces lavendulae increased with irrigation. The abundance of Streptomyces lavendulae decreased, and that of Arthrobacter spp. increased, with the incorporation of ginseng powder. Aerial growth of ginseng was promoted by irrigation, and ginseng root rot increased with the incorporation of ginseng powder. Conclusions: Ginseng root residues in the soil affected soil nutrients and microorganisms, and promoted ginseng root rot, but did not affect the aerial growth of ginseng.