• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.94-100
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• 1999

To establish the mass propagation system of Lavandula spica cv. Marino, shoot tip, node, internode and leaf segment cultures were carried out. RAPD was applied to detect the somaclonal variation. Callus induction was very high in the medium supplemented with 1 mg/l 2.4-D, 2 mg/l NAA. especially and combined with 0.05 mg/l BAP from leaves. Shoot formation was high with $2{\sim}4\;mg/l$ BAP or 4 mg/l BAP + 0.2 mg/l NAA from shoot tip. Shoot proliferation was 9.1 times in the $B_{5}$ medium with 0.5 mg/l BAP and 0.01 mg/l NAA. Root formation was improved in NAA, which was the concentration of 0.1 to 1 mg/l and 1 mg/l IAA. Nursery survival rate was enhanced over 90% and growth was looked good in the acclimation soil consisting of peatmoss : vermiculite : perlite (1:1:1, v:v:v). Randomly amplified polymorphic DNA banding patterns based on polymerase chain reaction (PCR) were used to assess the genetic variation in plants regenerated from in vitro culture.

• Korean Journal of Environmental Agriculture
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• v.2 no.1
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• pp.13-17
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• 1983

The influence of planting time and age of reclaimed fields on the contents of heavy metals in vegetable crops in suburban areas was investigated. Investigated crops were 4 radish cultivars, Chinese cabbage, spinach, Garland chrisanthemum, and Welsh onion. Cd, Pb, Cu, and Zn were analyzed. The results obtained were as follows. 1) For radishes except Altari-mu and Welsh onion, the highest concentrations of heavy metals were recorded in roots whereas, for Chinese cabbage and Garland chrisanthemum, the highest concentration was appeared in leaves. 2) For lettuce, radishes, and spinach, the lowest concentrations of heavy metals were recorded in winter and the concentrations were the same between spring and autumn. 3) In lettuce difference of concentrations of heavy metals were not appeared between field age after reclamation with sewege sludge. 4) The concentrations of heavy metals observed was not harmful to human health.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.2
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• pp.143-148
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• 1992

The antimutagenic effects of green-yellow vegetables toward aflatoxin B$_1$(AFB$_1$) and 4-nitroquinoline-1-ox-ide (4-NQO) using the Ames assay system with Salmonella typhimurium TA98 and TA100 were studied. Forty six to fifty percent of the methanol extracts of the vegetable samples inhibited the mutagenicity induced by AFB$_1$in TA98 and TA100. Perilla leaf, lettuce, broccoli, crown daisy, water dropwort, small water dropwort, red pepper, red pepper leaves, amaranth, spinach and radish root were significantly reduced the mutagenicity of AFB$_1$(p< 0.01). Whereas 25 out of 27 samples (93%) exhibited antimutagenicity toward a direct mutagen of 4-NQO (p< 0.01. 0.05). The samples which showed the strong antimutagenicity (>60%) were cabbage, kale, lettuce, broccoli, mustard leaf, green red pepper, green sweet pepper, spinach, amaranth, soybean sprout and immature pumpkin. The juices from the several samples also showed antimu-tagenic activity toward AFB$_1$. Cabbage, perilla leaf, small water dropwort and spinach reduced TAT100 revertants dose dependently in the range of 50-500$m\ell$/plate, however, cucumber and carrot showed little effect.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.74-82
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• 2014

A perennial plant species of Ranunculaceae, Pulsatilla tongkangensis is a very rare species only found in limestone vegetation of South Korea. It is not only rare but also has great value as a horticultural resource, thus resulting in serious problems of overexploitation and habitat disturbance. For the conservation of the rare species and its development as a resource, the study was conducted with the following three aims: first, to investigate any new geographical distribution of the species; second, to identify the site condition and vegetation structure of its habitat; and third, to compare the ecology of P. tongkangensis in other microhabitats such as rock, slope and ridge area. A new distribution was found in limestone vegetation at Danyang-gun, Chungcheongbuk-do. It was, therefore, confirmed that the distribution of P. tongkangensis exactly coincides with the area of distribution of limestone, indicating a possibility that the species might be a calcicole. 87% of the microhabitats found in rocks. Compared to P. tongkangensis, at ridges or slopes, number of P. tongkangensis plants were few in rocks. It is possibly due to the lack of microhabitats that could settle in rocky areas. Once established on rocks, however, plants grew large enough to flower with higher number of leaves and flowers per plant, resulting in higher flowering rate on rocky areas. Cracks in/between rocks are likely to be safe places for the plants with favorable light conditions, abundant fine particles to root down and reduced competition for resources. Yet, further research is necessary in terms of determining whether the species is indeed a calcicole, whether other limiting factors work in its establishment stage, and genetic diversity exists in the population.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.217-225
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• 2023

This experiment was conducted to investigate the effects of microwave irradiation on the growth and secondary metabolite contents of lettuce seedlings. Seedlings at three weeks after sowing were treated by a microwave oven for 0, 4, 8, and 12 seconds with 200 W. After cultivation in a close plant production system for 4 weeks, plant growth measurements and secondary metabolite analysis were performed. The results showed that the fresh and dry weights of the shoot and root, leaf area, leaf length, and the number of leaves were decreased as increasing the microwave treatment times. Chlorophyll a and b, total carotenoids were increased and total phenolics were decreased at the 12-second treatment compared to the 4-second treatment. Total flavonoid contents were decreased at the 8-second treatment compared to the control. These results suggest that the changes in the levels of secondary metabolites were caused by oxidative stress. Although there was no significant difference in secondary metabolite contents excluding total flavonoid contents on the microwave treatments compared to the control, the significant difference suggests that the microwave treatment of 200 W and 2.45 GHz may alter secondary metabolite contents of lettuce after 4 weeks.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.6
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• pp.111-119
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• 2018

This study was carried out to investigate the growth characteristics and propagation methods of the Korean native Amelanchier asiatica, A. arborea, and A. alnifolia as fruit trees for gardens. Due to the lack of recent research on Amelanchier spp., their superficial classification is still unclear and the names are being used interchangeably. The results are obtained as follows : A. arborea and A. alnifolia were globular type multi-stemmed shrubs. A 20-year-old tree of A. asiatica was 7.8m in height, with a 5.2m crown width, with one trunk. As for the morphological characteristics, leaves of A. asiatica were oblong, with an acuminate of, 6.1cm and 3.6cm width, but A. arborea and A. alnifolia had acute obovate leaves. The leaf size of A. alnifolia was the largest among the three species. The flower size of A. asiatica was bigger than that of A. arborea and A. alnifolia. In addition, its petals and flower clusters were also the largest among the three species. The flowering of A. asiatica initiated on April 21 and then bloomed for a duration of 24 days in Osan, while that of A. arborea and A. alnifolia initiated flowering on April 12 and then bloomed for a duration of 22 days in the same location. The fruit of A. arborea and A. alnifolia were green on May 10~12, it changed into purplish red on May 24~26, and its matured on June 1~3. The duration of fruit persistence of A. arborea and A. alnifolia were 48~50 days. On the other hand, A. asiatica showed greenish fruit on May 20, it became red on September 4, and had fallen by October 3. The fruit size was the largest at 1.03cm of height and 1.12cm of diameter in the A. arborea, followed by the big berry of A. alnifolia and the smallest fruit in the native, A. asiatica. It was difficult to root due to the hardwood cutting of A. arborea at a 40% rate of rooting. In the softwood cutting, the rooting rate of A. arborea was increased by the treatment with concentrated IBA, especially at 5,000 and 7,000ppm. The optimum date for cutting was on June 27, when the rooting rate was more than 80%. The most effective method for rooting of A. arborea was rootone or 7,000 ppm IBA treatment on June 27 softwood cuttings, which showed a rooting rate of over 80%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.7
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• pp.975-979
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• 2010

Biological activities of different parts (stems, leaves, roots, fruits) and solvents (water, ethanol) of seabuckthorn (Hippophae rhamnoides L.) grown in Korea were tested as follows. In the experiment of inhibiting $\alpha$-glucosidase activity, ethanol extract of Hippophae rhamnoides L. stem showed the highest inhibitory activity by 93% and the next highest was the ethanol extract of its leaf by 88.7%. In the case of these two extracts, the effect of inhibiting $\alpha$-glucosidase activity was extraordinarily great when comparing with control group, acarbose. In the experiment of inhibiting $\alpha$-amylase activity, water extract of leaf showed the highest result by 54.7%, among all extracts. Regarding anticancer effect for HT-29 cell and DU-145 cell, water extract of root showed 47.1% and 32.3% activities, respectively. The experiment on antibacterial activity showed that the ethanol extract from the leaf inhibitory activity of Clostridium butyricum, Proteus mirabilis, and Shigella flexneri which are the several food borne pathogenic strains. In future research, materials for biological activity appear isolated and purified and research should continue.

• Current Research on Agriculture and Life Sciences
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• v.24
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• pp.23-27
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• 2006

Seedling characteristics of rice on nursery covered with different weight of polypropylene spunbonded fabric(PSF) were investigated to determine the optimum thickness of PSF for raising of healthy rice seedlings. Four different thickness of PSF 40, 60, 80 and $100g/m^2$ were used in the seedling nursery with a conventional polyethylene(PE) film. PSF and PE were taken off in 20 days after sowing(DAS) on April and in 10 DAS on June. Plant height of 20- and 30- day old seedlings in PSF covering nursery was slightly taller as the increase of PSF thickness from $40g/m^2$ to $100g/m^2$, but number of leaves and shoot dry-weight of the seedlings was reduced. Root solidity of seedlings grown in lighter PSF ($40{\sim}60g/m^2$) covering nursery was more favorable than that in relatively heavier PSF($80{\sim}100g/m^2$). The optimum thickness of PSF for the raising of healthy seedlings was $40{\sim}60g/m^2$ PSF at sowing on April 20 and April 30 for single cropping, and $40g/m^2$ PSF on June for double cropping based on the growth characteristics of seedlings investigated at seedling nursery covered with different thickness of PSF.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.9-13
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• 2008

The wasabi was planted in glasshouse to investigate the effect shade level (0, 10, 30, 70%) on growth and photosynthetic response. The net photosynthetic rate and stomatal conductance were the highest under 10% shading rate in greenhouse. The lowest transpiration rate was in the non-shading treatment. 70% shading rate significantly reduced in photosynthetic characteristics. The number of leaf was decreased with increasing of shading rate. Leaf and petiole weight was the highest 10% shading and then followed by the non-shading treatment. Number of leaves, leaf, petiole and total weight were severely declined in 70% shading treatment. Shading rate (0%, 10%, and 30%) did not significantly influenced on the leaf length, leaf width and root growth. 10% shading level was the most effective for wasabi leaf production in hydroponics.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.673-679
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• 2012

This study was conducted to examine the effect of artificial light source and photoperiod on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings were grown under 3 light sources, fluorescent lamp (FL, Philips Co. Ltd., the Netherlands), WL #1 (Hepas Co. Ltd., Korea), and WL #2 (FC Poibe Co., Ltd., Korea), each with 3 photoperiods, 12/12, 18/6, and 24/0 (Light/Dark). An irradiance spectrum analysis showed that FL has various peaks in the 400-700 nm range, while WL #1 and WL #2 have only one monochromatic peak at 450 and 550 nm, respectively. The greatest plant height, fresh and dry weights were obtained in the 24/0 (Light/Dark) photoperiod. The 24/0 (Light/Dark) photoperiod treatment promoted vegetative growth of the leaf area. Length of the longest root, number of leaves, fresh weight, and total anthocyanin contents were greater in FL than in either WL #1 or #2. The greatest chlorophyll fluorescence (Fv/Fm) was found in the 12/12 (Light/Dark) photoperiod with FL treatment. The energy use efficiency of the LED increased by about 35-46% as compared to FL. Results suggest a possibility of LED being used as a substitute light source for fluorescent lamp for lettuce cultivation in a plant factory system.