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Experimental study on the behavior of the adjacent ground due to the sidewall failure in a shallow tunnel (얕은터널에서 측벽파괴시 주변지반 거동에 대한 실험적 연구)

  • Park, Chan Hyuk;Lee, Sang Duk
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.19 no.6
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    • pp.871-885
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    • 2017
  • Nowadays, the construction of tunnels with a shallow depth drastically in urban areas increases. But the effect of sidewall displacement in shallow tunnel on its behavior is not well known yet. Most studies on the shallow tunnel have been limited to the stability and the failure of the tunnel and the adjacent ground in plane strain state. Therefore, the model tests were conducted in a model ground which was built with carbon rods, in order to investigate the impact of the tunnel sidewall displacement on the lateral load transfer to the adjacent ground. The lateral displacement of the tunnel sidewall and the load transfered to the adjacent ground were measured in model tests for various overburdens (0.50D, 0.75D, 1.00D, 1.25D). As results, if the cover depth of tunnel was over a constant depth (0.75D) in a shallow tunnel, the tunnel sidewall was failed with a constant shape not depending on the tunnel cover depth and also not affected by the opposite side of the wall. But, if the cover depth of tunnel was under a constant depth (0.75D), the failure of the tunnel sidewall could affect the opposite sidewall. In addition, if the displacement of tunnel sidewall with 50% of the critical displacement occurred, the tunnel failure was found to be at least 75%. However, additional studies are deemed necessary, since they may differ depending on the ground conditions.

Characteristics of Biocellulose by Gluconobacter uchimurae GYS15 (Gluconobacter uchimurae GYS15 균주로부터 생산되는 Biocellulose의 특성 확인)

  • Lee, Young Sun;Kim, Jae Young;Cha, Mi Yeon;Kang, Hee Cheol
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.3
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    • pp.247-255
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    • 2016
  • In order to select a strain that forms a Biocellulose (BC), strain producing acetic acid was selected from commercially available kombucha. Through SM broth it was confirmed that the strain is a gram negative bacteria in the form of rods having no motility through a phase contrast microscope. The result of phylogenetic inference analysis based on 16S rDNA sequence analysis for the identification of strains was most closely related to Gluconobacter uchimurae (G. uchimurae) and was named G. uchimurae GYS15 strain. The strain showed the highest degree of growth when cultured for 14 days under the conditions of pH 5 and $25^{\circ}C$. Moreover, it showed the highest degree of growth in a Glucose addition disaccharide as the optimum carbon source sucrose and fructose. Also, 0.5% NaCl, upon the addition of Malto extract, showed the highest degree of growth. Based on investigation by the optimum growth conditions to confirm the physical properties of BC obtained by culturing G. uchimurae GYS15 strains. The surface structure was observed through an scanning electron microscope (SEM) showed a high networks structure. It until $8.6{\pm}0.38$ times when the water holding capacity is re-absorbed and re-absorbed holding oil up to $6.6{\pm}0.51$ times confirmed. In conclusion, using these material properties, it was possible to confirm the possibility of a variety of cosmetic materials and mask pack materials.

In vitro and in vivo Evaluations of LB 10517, a Novel Parenteral Broad-Spectrum Cephalosporin

  • Song, Hye-Kyong;Nishino, Takeshi;Seo, Mi-Kyeong;Kim, Mu-Yong;Lee, Yong-Hee;Kim, In-Chull;Kwak, Jin-Hwan
    • Archives of Pharmacal Research
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    • v.19 no.1
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    • pp.46-51
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    • 1996
  • The in vitro activity of LB 10517, a new catechol-substituted cephalosporin, was compared with those of E-1077, cefpirome and ceftazidime 1034 clinical isolates collected in Japan. LB10517 showed a broad-spectrum antibacterial activity against a wide range of grampositive and gram-negative bacteria including non-glucose fermenting rods, Pseudomonas aeruginosa. Against the methicillin-susceptible strains of Staphylococcus aureus (MSSA) and Strptoccus pyogenes, the $MIC_{90}$ values of LB10517 which required to inhibit 90% of the strains wre $3.13\mug/ml\; and\; 0.1\mug/ml$, respectively. It was as active as E-1077 but more active than cefpirome and ceftazidime. Methicillin-resistant strains of S.aureus (MRSA) and Enterococcus spp. were highly resistant to all the test compunds. LB10517 was highly active against most members of the family Enterobacteriaceae, 90% of which were inhibited at a concentration of less than $0.78\mug/ml$, except for Enterobacter cloacae ($1.56\mug/ml$) and Serratia marcescens ($3.13\mug/ml$)Its activity was comparable to those of E-1077 and cefpirome but it was greater than that of ceftazidime. Against Pseudomonas aeruginosa, LB10517 showed the most potent antibacterial activity among the compounds tested. Ninety percent of P. aeruginosa isolates were susceptible at the concentration of $0.39\mug/ml$. Its activity was 32-to 128 fold higher than those of E-1077, cefpirome and ceftazidime. Against imipenem- or ofloxacin-resistant P. aeruginosa, LB10517 with $MIC_{90}\; of\; 6.25 \\mug/ml\; and\; 3.13\mug/ml$, respectively, showed 16-fold more potent activity than the other test compounds. LB10517 showed a relatively high plasma level and long plasma elimination half-life in rats $(t_{1/2}(\beta,\; 52 min)\; and\; dogs\; (t_{1/2}(\beta),\; 103 min)$.

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Studies on Thermophilic Flat-sour Bacteria in Soymilk: Isolation, Indentification and Determination of Heat Resistance (두유내의 고온성 Flat-sour 변질균의 분리.동정 및 열저항성에 관한 연구)

  • Chung, Je-Bong;Lee, Kyun-Hee;Sohn, Heon-Soo;Kim, Suk-Min
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.218-224
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    • 1988
  • For the optimization of thermal processing conditions in soymilk process, 4 strains of thermoresistant flat-sour bacteria were isolated from soymilk. The isolates were aerobic spore-forming rods, and grew at $-65^{\circ}C$. Based on the morphological and physiological properties, all of the isolated strains were identified as Bacillus stearothermophilus. The heat resistance of spores of 3 isolates and Bacillus stearothermophillus ATCC 12980 as a reference was determined in soymilk(pH 7.0) and pH 7.0 buffer solution. For each of the spores studied, linear regression equations with standard error were presented for the thermal destruction at 110, 115, 121, and $125^{\circ}C$. It was not obvious that the components of soy milk increased the heat resistance of spores. Between the strains studied, variability was noted in the D values at the different temperature, and no one strain was consistently the most heat resistant at all the given temperatures. The average D value for the 4 strains was 77.27, 20.20, 2.76 and 1.39 min at 110, 115, 121 and $125^{\circ}C$, respectively, and the average z value was $8.36^{\circ}C$.

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Studies on the ${\beta}-Tyrosinase$ -Part 1. On the Enzymological Characteristics of ${\beta}-Tyrosinase$- (${\beta}-Tyrosinase$에 관한 연구 -제1보, ${\beta}-Tyrosinase$의 효소학적(酵素學的) 성질(性質)에 대하여-)

  • Kim, Chan-Jo;Nagasawa, Toru;Tani, Yoshiki;Yamada, Hideaki
    • Applied Biological Chemistry
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    • v.22 no.4
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    • pp.191-197
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    • 1979
  • ${\beta}-Tyrosinase$ was purified and crystallized from cells of Escherichia intermedia A-21 grown in a medium supplemented with 0.2% L-tyrosine. Molecular weight of its subunit, Km value and absorption spectra were determined. Crystallization methods were also studied to eliminate any unnecessary procedures. The results obtained were as follows: 1. The purification procedure included ammonium sulfate fractionation, dialysis against potassium phosphate buffer, pH 6.0 and pH 7.0, and DEAE-Sephadex column chromatography. In the column chromatography, 11 mg of protein was applied per ml of DEAE-Sephadex for efficiency. 2. Steps of protamine sulfate treatment and Sephadex G-150 gel filtration could be eliminated for this enzyme from the known procedures. 3. The purified enzyme was dissolved in 0.01M potassium phosphate buffer containing 2-mercaptoethanol, with a concentration of 20mg/ml. Crystalline enzyme, which appears as hexagonal rods, was obtained by adding solid fine powdered ammonium sulfate to the enzyme solution. 4. Absorption maxima of the enzyme appeared at 340 and 430nm when associated with pyridoxal phosphate. 5. Km value of the enzyme for L-tyrosine was $2.31{\times}10^{-4}M$ and the molecular weight of its subunit was determined by SDS-polyacrylamide electrophoresis to be approximately 50,000.

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A Study on the Comparison of Brazed Joint of Zircaloy-4 with PVD-Be and Zr-Be Amorphous alloys as Filler Metals (PVD-Be와 비정질 Zr-Be 합금을 용가재로 사용한 Zircaloy-4의 브레이징 접합부의 비교 연구)

  • Hwang, Yong-Hwa;Kim, Jae-Yong;Lee, Hyung-Kwon;Koh, Jin-Hyun;Oh, Se-Yong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.2
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    • pp.113-119
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    • 2006
  • Brazing is an important manufacturing process in the fabrication of Heavy Water Reactor fuel rods, in which bearing and spacer pads are joined to Zircaloy-4 cladding tubes. The physical vapor deposition(PVD) technique is currently used to deposit metallic Be on the surfaces of pads as a filler metal. Amorphous Zr-Be binary alloys which are manufactured by rapid solidification process are under developing to substitute the conventional PVD-Be coating. In the present study, brazed joint with PVD and amorphous alloys of $Zr_{1-x}Be_{x}(0.3{\le}x{\le}0.5)$ as filler metals are compared by mechanism, microstructure and hardness. The thickness of brazed joint with amorphous alloys became much smaller than that of PVD-Be. The erosion of base metal did not occur in the brazed joint with amorphous alloys. The brazing mechanism for PVD-Be seems to be Be diffusion into Zr-4 with capillary action resulting from eutectic reaction while that for amorphous alloys are associated with the liquid phase formation in the brazed joint. The brazed joint microstructure with PVD-Be consists of dendrite while that with amorphous alloys is globular. The $Zr_{0.7}Be_{0.3}$ alloy shows the smooth interface with little erosion in the base metal and is recommended a most suitable brazing filler metal for Zircaloy-4.

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Fabrication of 2D Bravais Nano Pattern and Growth of ZnO Nano Rods with Photonic Crystal Effect (2차원 Bravais Lattice를 가지는 나노 패턴 제조 및 광결정 효과를 가지는 ZnO 나노 기둥 성장)

  • Kim, Tae-Un;Moon, Jong-Ha;Kim, Seon-Hoon;Kim, Doo-Gun;Kim, Jin-Hyeok
    • Korean Journal of Materials Research
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    • v.21 no.12
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    • pp.697-702
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    • 2011
  • Two-dimensional (2D) nano patterns including a two-dimensional Bravais lattice were fabricated by laser interference lithography using a two step exposure process. After the first exposure, the substrate itself was rotated by a certain angle, $90^{\circ}$ for a square or rectangular lattice, $75^{\circ}$ for an oblique lattice, and $60^{\circ}$ for a hexagonal lattice, and the $90^{\circ}$ and laser incident angle changed for rectangular and the $45^{\circ}$ and laser incident angle changed for a centered rectangular; we then carried out a second exposure process to form 2D bravais lattices. The band structure of five different 2D nano patterns was simulated by a beam propagation program. The presence of the band-gap effect was shown in an oblique and hexagonal structure. The oblique latticed ZnO nano-photonic crystal array had a pseudo-bandgap at a frequency of 0.337-0.375, 0.575-0.596 and 0.858-0.870. The hexagonal latticed ZnO nano-crystallite array had a pseudo-bandgap at a frequency of 0.335-0.384 and 0.585-0.645. The ZnO nano structure with an oblique and hexagonal structure was grown through the patterned opening window area by a hydrothermal method. The morphology of 2D nano patterns and ZnO nano structures were investigated by atomic force microscopy and scanning electron microscopy. The diameter of the opening window was approximately 250 nm. The height and width of ZnO nano-photonic crystals were 380 nm and 250 nm, respectively.

Leak Location Detection of Underground Water Pipes using Acoustic Emission and Acceleration Signals (음향방출 및 가속도 신호를 이용한 지하매설 상수도배관의 누수지점 탐지연구)

  • Lee, Young-Sup;Yoon, Dong-Jin;Jeong, Jung-Chae
    • Journal of the Korean Society for Nondestructive Testing
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    • v.23 no.3
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    • pp.227-236
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    • 2003
  • Leaks in underground pipelines can cause social, environmental and economical problems. One of relevant countermeasures against leaks is to find and repair of leak points of the pipes. Leak noise is a good source to identify the location of leak points of the pipelines. Although there have been several methods to detect the leak location with leak noise, such as listening rods, hydrophones or ground microphones, they have not been so efficient tools. In this paper, acoustic emission (AE) sensors and accelermeters are used to detect leak locations which could provide all easier and move efficient method. Filtering, signal processing and algorithm of raw input data from sensors for the detection of leak location are described. A 120m-long pipeline system for experiment is installed and the results with the system show that the algorithm with the AE sensors and accelerometers offers accurate pinpointing of leaks. Theoretical analysis of sound wave propagation speed of water in underground pipes, which is critically important in leak locating, is also described.

Design Optimization of Duplex Burnable Poison Rods and Feasibility Evaluation for Core Design (이중구조 가연성독봉 설계안의 최적화 및 노심 핵설계 타당성 평가)

  • Yoon Seok-Kyun;Lee Dae-Jin;Kim Myung-Hyun
    • Journal of Energy Engineering
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    • v.13 no.4
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    • pp.242-258
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    • 2004
  • The duplex burnable poison absorbers concept was suggested by Korea Atomic Energy Research Institute. This BP rod is composed of inner region of natural U-Gd$_2$O$_3$ and outer shell of enriched UO$_2$-Er$_2$O$_3$. It is expected that this burnable absorber has same reactivity control capability with gadolinia burnable absorber used in extened fuel cycle. In order to evaluate the nuclear feasibility of duplex BPs, the nuclear design characteristics were compared with that of four types of burnable absorbers; gadolinia, erbia, IFBA, dysprosia duplex BP on 24 months fuel cycle for Korean Standard Nuclear Power plants. According to the evaluation results of nuclear characteristics, the duplex BPs were better than other BPs on k-infinitives, reactivity holddown worth (RHW), pin power peaking and moderator temperature coefficient (MTC). The possibility of nuclear core design was also confirmed based on the optimized fuel assemblies which were searched for a sensitivity analysis. Characteristics of core design with duplex BPs was compared with that of reference core with gadolinia BPs for cycle length, power peaking and MTC. The duplex BP core had a little longer cycle length by 4 to 7 days because of increased amount of fissile in enriched uranium at the outer shell of duplex BP In case of power peaking F$\_$Q/ of duplex BP core was reduced from 1.5773 to 1.5335. MTC was also less -0.48 pcm/C than that of reference core. Finally, evaluation of fuel cycle economy was performed for the manufacturing feasibility test and fuel cost evaluation with duplex BPs. Fuel cycle economy of duplex BP core almost was equivalent with that of gadolinia BP core.

The Effect of Weld Line on the Mechanical Strengths and its Elimination Process in the Zr-4 Resistance Upset Welds (지르칼로이-4의 저항업셋용접에서 용접선이 기계적성질에 미치는 영향과 그 소멸과정)

  • Koh, Jin-Hyun;Lee, Jung-Won;Jung, Sung-Hoon
    • Nuclear Engineering and Technology
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    • v.23 no.1
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    • pp.1-11
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    • 1991
  • The objective of this study is to investigate the effect of weld line on the mechanical strengths and the process of weld line elimination in the Zircaloy-4 resistance upset welding for the fabrication of heavy water reactor fuel rods. The weld current and the amount of upset increased linearly with the main heat, in which two relations between them were derived. It was found that the threshold to obtain sound weld was 50% of main heat in terms of weld upset size, mechanical strengths and weld line elimination. The weld microstructure of resistance upset welds of Zircaloy-4 comprsied basketweave, Widmanstatten and martensite respectively by changing the main heats. Dimples on uniaxially fractured surface at weld line in the Zr-4 welds were larger and deeper compared with those on biaxially fractured surface. It was also found that the process of the weld line elimination in the resistance upset weld of Zircaloy-4 could be divided into three stages in terms of the presence of many pores, their shrinkage and elimination, and the shrinkage of the original weld interface with increasing weld currents.

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