• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.81-85
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• 2002

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Stenotrophomonas, but were not included in these genera. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium. We attempted to screening of bioactive compounds from oligotrophs which was isolated from forest soil. The active compounds were analyzed by mass and NMR spectrum, one of them identified as crisamicin A. Another one designated as SAPH is a new compound. The results indicate that there were possibilities for finding new compounds from the rare microorganisms such as oligotrophs.

• 한국발생생물학회지:발생과생식
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• 제15권3호
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• pp.239-247
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• 2011

Spermatogenesis and taxonomic values of mature sperm morphology of in male Septifer (Mytilisepta) virgatus were investigated by transmission electron microscope observations. The morphologies of the sperm nucleus and the acrosome of this species are the cylinder shape and cone shape, respectively. Spermatozoa are approximately 45-50 ${\mu}m$ in length including a sperm nucleus (about 1.26 ${\mu}m$ long), an acrosome (about 0.99 ${\mu}m$ long), and tail flagellum (about 45-47 ${\mu}m$). Several electron-dense proacrosomal vesicles become later the definitive acrosomal vesicle by the fusion of several Golgi-derived vesicles. The acrosome of this species has two regions of differing electron density: there is a thin, outer electron-dense opaque region (part) at the anterior end, behind which is a thicker, more electron-lucent region (part). In genus Septifer in Mytilidae, an axial rod does not find and also a mid-central line hole does not appear in the sperm nucleus. However, in genus Mytilus in Mytilidae, in subclass Pteriomorphia, an axial rod and a mid-central line hole appeared in the sperm nucleus. These morphological differences of the acrosome and sperm nucleus between the genuses Septifer and Mytilus can be used for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The number of mitochondria in the midpiece of the sperm of this species are five, as seen in subclass Pteriomorphia.

• Nuclear Engineering and Technology
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• 제50권3호
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• pp.504-511
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• 2018

In Lithuania, all spent nuclear fuel (SNF) resulted from the operation of the Ignalina Nuclear Power Plant (INPP), which had two Russian Acronym for "Channelized Large Power Reactor"-type reactors. After the final shutdown, the total amount of SNF at the INPP was approximately 22,000 fuel assemblies. All these assemblies will be stored for about 50 years and disposed of after that. The decision to shut down and decommission both reactors in Lithuania before termination of design period raises a significant challenge for the treatment of accumulated SNF. Therefore, various techniques and technologies for SNF management were developed and justified for that specific case, and a set of special equipment was installed at the INPP, the effectiveness of which was demonstrated during its operation. This article presents unique techniques related to the management of SNF adopted and commissioned at the INPP after its operation shutdown, namely fuel rod cladding leak tightness control system and special equipment for collection of possible spillage during handling of SNF assembly in the hot cell. The operational experience and measurement results of fuel rod cladding leak tightness control system are presented.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1995년도 추계학술발표회논문집(1)
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• pp.480-486
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• 1995

Turbulent flow field in a subchannel of bare rod bundles has been numerically simulated using the control volume based finite element method. Launder & Ying model of Reynolds stress and Lam & Bremhorst low-Reynolds number model are implemented in k-$\varepsilon$ equations and momentum equations. Secondary flows are simulated using the stream function and vorticity approach. The control volume based finite element method enable to use the upwind scheme (donor cell scheme). Sensitivity of the constants in the models are studied, and proper values are found to get the close result to the measured flow distributions.

• 환경생물
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• 제18권4호
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• pp.441-446
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• 2000

Trichoderma harzianum is an aggressive causal agent of green mold disease on mushroom cultivation. Some bacterial strains isolated, from oyster mushroom compost in Wonju, were found to have in vitro antifungal activity against Trichoderma harzianum ATCC 6385, 6504, and our isolates Trichoderma spp. Y and G. Further in vitro antifungal studies on several strains of phytopathogenic fungi showed that all of 12 phytopathogenic fungal strains were significantly inhibited by the isolated antifungal bacteria in Petri dishes. Of these, KATB 99121 showed the broadest inhibiting effect and displayed as negative coagulase, negative sulfide production and rod shape. KATB 99121 was resistant to ampicillin, chlorampenicol, and kanamycin. Identification of isolates was determined by Biolog GN system, and KATB 99121 was identified as Photobacterium logei because of 96 probability, 0.65 similarity, and 4.97 disturbance. With electron microscopy, thin section of KATB 99121 strain revealed typical rod-like shaped cell (0.6-0.8${\mu}{\textrm}{m}$$\times$1.5-2.0${\mu}{\textrm}{m}$) with prokaryotic structure and organization.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.36-36
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• 2003

Goldfish retina has been well studied to a great extent. In spite of that, electrical characteristics of dissociated horizontal cells(HCs) have not been identified in detail. Thus the cone-and the rod- HCs dissociated from goldfish retina were investigated electrophysiologically using whole-cell patch-clamping recording. To explore the basic electrical property, We examined voltage-dependent channels in all types of HCs. For the futher understanding of GABAergic pathway, the localization and distribution of GABA receptors was examined in cone- HCs including HC axon terminals(ATs).

• Tribology and Lubricants
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• 제36권3호
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• pp.168-192
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• 2020

In recently designed diesel engines, the running conditions for piston-pin bearings have become severe because of the higher combustion pressure and increased temperature. Moreover, the metal removal from the bushing material has strongly reduced the ability of the antifriction material to accept asperity contacts. Therefore, it is necessary to find ways of reducing wear scar on the connecting-rod small-end bushing and piston-pin boss bearing related to the higher combustion pressure on the power cell of an engine. In this work, the position and level of material removal from the surfaces of the bushing and bearing under such severe operating conditions - for example, maximum power and torque conditions of a passenger car diesel engine - are estimated for several combinations of surface roughness. First, piston-pin rotating motion is investigated by calculating the friction coefficient at piston-pin bearings, the oil film thickness, and the frictional torques induced by hydrodynamic shear stress. Subsequently, the wear scarring on the surfaces of a connecting-rod small-end bushing and two piston-pin boss bearings related to piston-pin rotational motion is numerically calculated under the maximum power and torque operating conditions. This work is helpful to determine the reasonable surface roughness of the bushing and bearing for reducing wear volume occurring at the interface between a bearing and a shaft.

• Applied Microscopy
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• 제19권2호
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• pp.119-137
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• 1989

Turtle bladder의 상피조직(上皮組織)과 세포막(細胞膜) 투과성(透過性)을 분석하기 위하여 동결절단법(凍結切斷法)을 적용(適用)하여 전자현미경(電子顯微鏡) 관찰을 하였으며 그 결과 다음과 같이 요약(要約)된다. 1. 방광상피(膀胱上皮)의 3가지 주요세포형(主要細胞形)은 granular-cell, ${\alpha}$ 및 ${\beta}$형(形)의 CA-rich cell로서 구분된다. 2. 과립성세포(顆粒性細胞)의 주요기능(主要機能)은 $Na^+$ 재흡수(再吸收)이며, 두 단계(段階)의 수송과정(輸送過程)으로 설명되며 정단세포막(頂端細胞膜)을 통한 $Na^+$의 세포내(細胞內) 확산이동(擴散移動)과 그후 기저막(基底膜)에 위치한 $Na^{+}\;-K^{+}$ 펌프에 의한 능동수송과정(能動輸送過程)이다. 3. ${\alpha}$ 및 ${\beta}$형(形) CA-rich cell은 $Na^+$ 수송(輸送)에 관여하지 않으며, ${\alpha}$형(形)의 CA-cell은 정단세포막(頂端細胞膜)의 proton펌프를 이용하여 proton 분필(分泌)에 관여한다. 또한 ${\beta}$형(形)의 CA-cell는 정단세포막(頂端細胞膜)을 통한 $HCO_{3}^-$ 분필수송(分泌輸送)의 기능을 가지고 있다. 4. 동결절단법(凍結切斷法)의 적용하에 세포막표면(細胞膜表面)의 특성(特性)을 관찰한 바, ${\alpha}$형(形)의 CA-cell의 정단세포막(頂端細胞膜)은 proton펌프를 함유하는 것으로 보이는 intramembrane particle이 다수 관찰되고 있으며, ${\beta}$형(形) CA-cell은 기저세포막(基底細胞膜)에서 이와같은 intramembrane particle이 나타나고 있다. 5. 상술(上述)한 두 type의 CA-cell에서 수송특성(輸送特性)의 차이는 proton 및 $HCO_{3}^-$ 분필수송(分泌輸送)이 서로 반대의 방향으로 일어나는 것으로 사료된다.

• 대한기계학회논문집A
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• 제38권4호
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• pp.443-449
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• 2014

조사시험 도중 핵연료의 특성변화를 확인하기 위하여 원자로 수조내에 위치한 조사리그로부터 핵연료봉을 분리한 후 핫셀로 이송하여 중간검사를 진행한다. 또한 중간검사를 마친 핵연료봉은 원자로의 작업 수조로 이동시켜 조사리그에 재장착을 하게 되며, 재조립된 조사리그는 원자로 노심의 조사리그에 장착시켜 조사시험을 계속 진행한다. 그러나 중성자 조사가 진행된 핵연료봉은 높은 에너지의 방사선을 방출하기 때문에 작업자가 방사선에 피폭되지 않게 하기 위하여 핵연료봉 장탈착 공정은 원자로 작업수조수 내에서 이루어져야 한다. 특히 조사리그의 길이가 5.4 미터이고, 핵연료봉의 장탈착 작업이 이루어질 하나로 작업수조수의 깊이는 6 미터로 매우 깊어 장탈착 작업을위한 특수한 장치가 필요하다. 본 연구에서는 중간검사가 가능한 새로운 핵연료봉 조립체를 설계하고, 조사 핵연료봉 조립체의 장탈착용 툴을 개발하여 노외 성능시험을 통해 그 성능을 검증하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.12-16
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• 1999

Xenorhabdus nematophilus sp., an insect-pathogenic bacterium, was newly isolated from Korean entomopathogenic nematode of Steinernema carpocapsae, which can be used as a useful bioinsecticide. Primary and secondary form variants of Xenorhabdus nematophilus were observed when cultured in vitro. Primary form variants adsorbed bromothymol blue, while secondary form did not. However, many other characters of two variants were very similar. The variants were all rod-shaped and cell size was highly variable ranging from 0.5 by 2.0 ${\mu}$m to 1.0 by 5.0 ${\mu}$m. Both produced highly toxic substances and killed the insect larva within 20∼38 hr, indicating that insect pathogenicity of Xenorhabdus is not directly associated with its phase variation. In addition, cell-free culture supernatant of Xenorhabdus was sufficient to kill the insect larva by injecting it ito insect hemolymph; however, cell-harboring culture broth was more effective for killing the insect. The use of Xenorhabdus nematophilus may provide a potential alternative to Bacillus thuringiensis (Bt) toxins.