• 원예과학기술지
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• 제34권5호
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• pp.665-676
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• 2016

본 연구에서는 기후변화로 인한 온도상승에 따른 과실 착색 불량 등의 문제를 해결하는데 필요한 기초자료를 제공하고자 고온에 의해 과피에서 발현되는 유전자들의 발현 양상 특성을 분석하였다. '하례조생'과 '부지화' 감귤 과실을 숙기 별로 수확하여 온도 조건(25, 30, $35^{\circ}C$)을 처리하고 당대사, 과피 착색, 세포벽 연화에 관련된 유전자들의 발현을 확인하였다. 유전자들은 '하례조생'과 '부지화'에서 각각 다른 양상으로 발현하였는데, beta-amylase(BMY), phenylalanine ammonia-lyase(PAL), chalcone synthase(CHS), flavanone 3-hydroxylase(F3H) 등의 유전자 발현은 대체적으로 유도되었고, polygalacturonase(PG) 유전자는 발현이 감소되는 경향이었다. '하례조생'은 과피 착색과 관련된 유전자인 CHS와 F3H는 성숙이 진행된 2-3단계에서 $25^{\circ}C$에 비해 고온에서 유전자 발현이 감소하였으며, PAL과 stilbene synthase(STS) 유전자는 $25^{\circ}C$에 비해 $30-35^{\circ}C$ 처리구에서 유전자 발현이 증가하였다. 2-3단계의 '부지화'에서는 BMY 유전자가 $25^{\circ}C$에 비해 $30-35^{\circ}C$에서 유전자 발현이 증가하였으며, F3H와 STS 유전자의 발현은 과실 성숙단계에서 모두 감소하는 경향이었고 온도의 영향은 크지 않았다. 성숙 1, 2단계에서 유전자의 발현 양상은 두 품종 모두에서 대체로 비슷한 경향이었는데, 3단계에서 '부지화' 과실의 유전자 발현은 '하례조생'과는 다르게 감소하는 경향이었다. 성숙이 진행되는 감귤류인 '하례조생'에서 '부지화'에 비해 7종류의 유전자의 발현이 많았으며, 고온에 따른 반응의 차이도 크게 나타났다. 본 연구에서 도출한 결과를 바탕으로 과실의 전사체를 분석함으로써 고온에 의한 감귤 과실의 성숙불량 문제를 이해하는 주요한 정보를 획득할 수 있을 것이다

• 한국작물학회지
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• 제44권1호
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• pp.59-63
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• 1999

Yield and growth characteristics were compared for five rice cultivars; a new Tongil-type, so called "super-rice", Dasanbyeo, an old Tongil-type Milyang 23, two japonicas Dongjinbyeo and Ilpumbyeo, and a new plant type (NPT)line IR65600-27-1-2. The objective of this stusy was to clarify the high yielding capacity of Dasanbyeo in terms of growth characteristics. The average grain yield (9 t/ha) of Dasanbyeo was higher than that of Milyang 23 by ca. 9% that of japonicas by 20 to 30%, and that of NPT line by ca. 100%. The higher grain yield of Dasanbyeo was attributable not only to the greater dry matter production but also to the higher harvest index (HI). Dasanbyeo showed the greatest dry matter at harvest owing not only to the rapid leaf expan-sion at early growth stage and the resulting high LAI through the entire growth stage but also to the high NAR despite the high LAI. The rapid leaf expansion of Dasanbyeo at early growth stage seemed to be related in part to the profuse tillering capacity. HI was 0.53 in Dasanbyeo, 0,51 in Milyang 23, 0.41 in japonicas, and 0.35 in NPT line. Dasanbyeo was indebted for its higher HI to the relatively high grain filling ratio in spite of a much greater sink size than the other cultivars. Dasan had a greater source to sink ratio during grain ripening as measured by LAD/spikelet and dry matter production/spikelet which showed positove correlations with the grain ripening ratio. New plant type (NPT) line showed the lowest grain yield owing to the small sink size and the low grain filling ratio which seemed to have resulted from the abundant occurrence of weak-strength spikelets. The weak sink strength, in turn, seemed to have suppressed photosynthesis during the grain ripening stage.

• 한국작물학회지
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• 제37권6호
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• pp.541-549
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• 1992

벼 담수표면직파재배시 파종기 및 품종을 기상 및 재배 환경과의 관계를 구조적으로 분석하여 담수직파에서 흔히 발생하는 도복의 방상대책과 후기등숙생리를 검토한 결과는 아래와 같다. 1. 초기생육형수질중에서 출아일수는 조기파종일수록 길었으며 단립면적당 입묘수 및 입묘율은 파종이 늦을수록 그리고 만생종일수록 많고 양호하였다. 2. 단립면적당 수수는 파종이 늦을수록 감소하였으며 유효경비율은 만파인 5월 21일 파종구에서 가장 낮았다. 출수개화일수는 파종기가 늦을수록 단축되었으며 등숙기간중 강우분포는 조생종의 등숙기에 많았고 중ㆍ만생종의 등숙기에는 비교적 적었다. 파종기별 및 품종의 숙기별 포장도복정도는 파종이 빠를수록, 그리고 숙기가 빠를수록 컸다. 5. 수량 및 수량구성형질은 파종이 빠를수록 많고 양호했으며 특히 등숙비율은 그차이가 뚜렸하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.340-344
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• 2005

In high-income Asian countries like Korea and Japan, per capita rice consumption has declined because of the change in consumer' favorite foods from rice to high-cost quality foods. This has forced farmers to reduce rice production. Although fodder rice could be another option to be cultured by farmers, available information concerning rice grain silage has been limited. In the present study, therefore, the difference in the quality of fodder rice silage prepared from either whole crop or grain at different ripening stages was compared. Various supplements were also added into whole crop and grain silages of fodder rice before ensiling, and thereafter, the palatability of prepared silages was determined by beef cattle. At ear emergence stage, the pH values for both grain and whole crop silages were approximately 4.5. In both grain and whole crop silages, the pH values were significantly increased by progressing ripening stages from milk-ripe stage to yellow-ripe stages, and the increase in pH value for grain silage was faster than that for whole crop silage. In the grain silage, the higher lactic acid (LA) content in grain silage seemed to be, the lower pH value was. Both in grain and whole crop silages, pH was significantly decreased by supplementation with LA bacteria. There were no significant differences in feed intake among any treatment groups.

• 한국응용과학기술학회지
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• 제30권2호
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• pp.348-355
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• 2013

오징어 젓갈에 오징어 먹즙을 2% 및 4% 농도로 첨가하고 $10^{\circ}C$에서 8주일간, $20^{\circ}C$에서 32일간 숙성시키면서 아미노태 질소와 근육단백질 변화를 분석한 결과는 다음과 같다. 오징어 먹즙이 첨가되지 않은 오징어 젓갈의 아미노태 질소는 식염 농도가 낮고 숙성온도가 높을수록 숙성 후반까지 계속 유의성 높게 증가하여 숙성이 촉진되었으며 오징어 근육의 단백질 변화는 myosin heavy chain이 숙성 초반에 현저히 분해되지만 actin의 변화는 거의 없어서 protease에 강하였다. 오징어 먹즙을 첨가한 오징어 젓갈의 아미노태 질소 함량은 숙성후반까지 계속 증가하였으나 증가폭은 무 첨가군에 비하여 적었으며 오징어 근육 단백질 중 myosin heavy chain은 숙성 중반에 현저히 분해되었으며 식염농도가 높고, 온도가 낮은 먹즙 첨가군은 분해 속도가 느렸다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.227-227
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• 2017

This study was conducted to analyze the reduction of quality and ripening rate of rice due to submergence during ripening stage and identify the physiological cause. Korean japonica type rice cultivars, Nampyeong was used in the experiment. The following 7, 14 day after heading, they were then moved into submergence treatment facility and we conducted the tests under three different submergence conditions - T1(clear water and overhead submergence), T2(Muddy water and exposure of Flag leaf end ), T3(Muddy water and overhead submergence), and the treatment lasted for 4days. The decrease of ripening rate was most severe in T3 treatment at 7days after heading, and the decrease of head rice ratio was most severe in T3 treatment at 14days after heading. Meanwhile the starch synthesis was inhibited, as the supply of assimilation products was inhibited in grain during the submergence treatment. And in stem, sucrose content was increased because the soluble carbohydrates accumulated before heading were decomposed. These changes may be due to the consumption of respiratory substrate in anaerobic conditions and the inhibition of the production of photosynthetic products by light interception. In order to see what kind of reaction occurs at the molecular level, we examined the degree of RNA expression in grain, stem and leaf. First, the expression of rna associated with starch synthesis in grain was decreased in all submergence treatment. and especially Ospul(DBE) was more decreased in 14days after heading treatment than 7days after heading treatment. This difference can explain the result that the decrease of head rice ratio was more severe at 14days after heading. And in stem, the expression of rna associated with the supply of assimilation products was decreased in submergence treatment. Finally in leaf, the expression of rna(ADH, ALDH) associated with anaerobic respiration was increased, while the expression of rna associated with photosynthesis was decreased. These results of physiological analysis can used to develop the cultivation technique and to offer the information for breeding the cultivars with tolerant characteristics to submergence stress during ripening stage in rice.

• 한국식품과학회지
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• 제21권6호
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• pp.863-868
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• 1989

한국산 양다래 주요 품종인 Hayward, Abbott, Bruno의 이화학적 특성을 조사하였던 바 결과는 다음과 같았다. 가용성 고형물, 조지방, 경도 및 비중은 Abbott가 가장 높은 값을 나타냈으며, 양다래에 함유된 무기물 성분은 K, P, Ca 및 Mg가 주된 성분들이며, 이 중 K와 Mg는 Bruno에 가장 많이 함유되어 있었다. 또한 유기산 중 아스코르브산, 말레산, 시트르산과 푸마르산 역시 다른 품종에 비하여 Bruno가 높은 함량을 보였다. Hayward 품종의 숙도에 따른 이화학적 특성을 조사하였던 바 숙성이 진행되는 동안 유리당 중 글루코오스와 프럭토오스의 함량이 증가된 반면 수우크로스는 감소되는 경향을 보였으며, 유기산의 경우 숙성이 진행되면서 말산과 시트르산은 감소한 반면 말레산, 푸마르산 그리고 숙신산 등은 그 양이 증가하는 경향을 보였다. 또한 전기영동에 의한 단백질 변화 양상을 숙성 정도에 따라 비교 하였던바 분자량 17,000~23,500사이에 새로운 단백질 띠가 나타났다.

• 동아시아식생활학회지
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• 제26권5호
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• pp.445-449
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• 2016

In this study, Red pearl (4x) and MBA (2x) were crossed Gangwon-do Agricultural Research and Extension Services in 2000 to compare the brewing characteristics of Cheonghyang, a seedless kind of grape registered in 2009, by maturity. Fruit characteristics of Cheonghyang were examined after the first racking and 300 days after fermentation during aging in manufacturing wine based on maturity categories of being immature, mature, and overmature after harvest between August 25 and 27, 2014. The soluble solid matter content did not differ between the first racking following manufacturing wine and during ripening, and the alcohol content following the first racking was 12.9% for immature ones, 13.0% for mature ones, and 12.7% for overmature ones., The alcohol content decreased by appropriately 1.3% to 1.8% during ripening. The total acidity content was 0.9% for immature ones, 0.8% for mature ones, and 0.5% for overmature ones following the first racking and showed insignificant variations during ripening of 0.8%, 0.7%, and 0.5%, respectively, with wine made from mature ones showing proper acidity. The volatile acid content following the first racking during ripening, showing insignificant variation by maturity; however, it decreased after ripening. The sensory evaluation detected differences in acidity of wine between mature and overmature grapes. There was no difference in sour taste between immature and mature grapes; however, overmature ones had low levels of sour taste. Our results showed that mature wine had the best overall preference.

• 한국작물학회지
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• 제60권4호
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• pp.412-420
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• 2015

Even though phosphorus (P) is essential element for plant growth and development, it is not enough for crop production in soil. To breed more P deficient tolerance rice, screening and selection in rice population is needed. We tried to develop more simple and rough screening method for breeding of P deficient tolerance rice. In P deficient condition, tiller number was dramatically decreased among yield components in rice. Though this result, we confirmed tiller number could be the best marker in screening of P deficient tolerance rice. 480 rice genetic resources were cultivated in rice bed tray filled with P deficient soil for four weeks and each dry weight was measured. Among them, the 55 kinds of genetic resource were selected then cultivated in paddy field with 3 fertilizer conditions. Plant dry weight and tiller number in ripening stage were shown significant difference according to P condition. Plant dry weight and tiller number in ripening stage was highly correlated especially in P deficient condition. Furthermore, the tiller number in ripening stage and plant dry weight in rough screening were shown high degree correlation. Though these results, we might expect measuring of plant dry weight after cultivation in rice bed tray filled with P deficient soil could be a simple and effective screening method in selection of P deficient tolerance rice.

• 한국식품저장유통학회지
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• 제14권5호
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• pp.511-515
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• 2007

Pre-climacteric 단계의 '대석조생' 자두(Prunus salicina L.)를 착색정도 따라 3단계로 구분하여 1-Methylcyclopropene (1-MCP, $1\;{\mu}L\;L^{-1}$ for 24 hours at $10^{\circ}C$)을 처리한 후 $10^{\circ}C$에서 유통시키면서 에틸렌 생성 량, 호흡량 경도, 산도, 색도 변화 등의 품질변화를 관찰하였다. 1-MCP처리에 의한 에틸렌 생성과 호흡량 감소가 모든 숙기에서 유의성이 인정되었다. 또한 1-MCP는 숙기와 관계없이 경도, 산도, 색도를 유지시키고, 가용성 고형물함량에는 아무런 영향을 미치지 않아, 악영향 없이 자두의 품질유지 효과를 나타내었다. 따라서 1-MCP를 적용하면 자두의 유통기간을 연장 할 수 있으므로 수확시기를 늦춰 맛있는 자두를 수확하여 유통시켜 소비자에게 좀더 맛있는 자두를 공급할 수 있을 것으로 예상되었다.