• Title/Summary/Keyword: ripening stage

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Gene Expression as Related to Ripening in High Temperature during Different Coloration Stages of 'Haryejosaeng' and 'Shiranuhi' Mandarin Fruits (온주밀감 '하례조생'과 '부지화' 과실의 착색 단계별 고온에 의한 성숙 관련 유전자의 발현 변화)

  • Ahn, Soon Young;Kim, Seon Ae;Moon, Young-Eel;Yun, Hae Keun
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.665-676
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    • 2016
  • As high temperature during citrus growing season has caused a serious problems including inferior coloration in production of mandarins in Korea, we were to investigate the expression pattern of several genes related with coloration during the ripening in high temperature condition of citrus fruits. The expression of genes related with sugar metabolism, cell wall degradation, and flavonoid synthesis in high temperature conditions was investigated in fruits of 'Haryejosaeng' (Citrus unshiu) and 'Shiranuhi' mandarin (C. reticulata). While the expression of beta-amylase (BMY), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) was differently induced, expression of polygalacturonase (PG) decreased dependently on temperature conditions. In 'Haryejosaeng' mandarin, while the expression of genes related to the skin coloration, such as CHS and F3H genes increased at $25^{\circ}C$, the expression of PAL and stilbene synthase (STS) genes were induced at $30-35^{\circ}C$ in all ripening stages. In 'Shiranuhi' mandarin, the expression of the BMY gene decreased at early time point in all temperature condition and then increased at $30-35^{\circ}C$ than at $25^{\circ}C$ in the ripening stage 2 to 3 of fruits. F3H and STS genes also showed the tendency to decrease at $30-35^{\circ}C$. Although the expression levels of genes in ripening stage 1 and stage 2 of fruits showed similar patterns in both 'Haryejosaeng' and 'Shiranuhi', the expression levels of genes were down-regulated in late ripening stage of 'Shiranuhi' fruits compared to 'Haryejosaeng'. In general, the mRNA levels of seven tested genes were higher in 'Haryejosaeng' than in 'Shiranuhi' mandarin, and expression of genes by high temperature was regulated sensitively in 'Haryejosaeng' compared to 'Shiranuhi' mandarin. Further investigations of expression of various genes based on transcriptome analysis in early ripening stage can provide valuable information about the responses to climatic changes in ripening citrus fruits.

Comparison of Yield and Growth Characteristics of Korean High Yielding Cultivars and IRRI's New Plant Type Rice Line

  • Lee, Byun-Woo;Ha, Jong-Ryuk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.1
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    • pp.59-63
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    • 1999
  • Yield and growth characteristics were compared for five rice cultivars; a new Tongil-type, so called "super-rice", Dasanbyeo, an old Tongil-type Milyang 23, two japonicas Dongjinbyeo and Ilpumbyeo, and a new plant type (NPT)line IR65600-27-1-2. The objective of this stusy was to clarify the high yielding capacity of Dasanbyeo in terms of growth characteristics. The average grain yield (9 t/ha) of Dasanbyeo was higher than that of Milyang 23 by ca. 9% that of japonicas by 20 to 30%, and that of NPT line by ca. 100%. The higher grain yield of Dasanbyeo was attributable not only to the greater dry matter production but also to the higher harvest index (HI). Dasanbyeo showed the greatest dry matter at harvest owing not only to the rapid leaf expan-sion at early growth stage and the resulting high LAI through the entire growth stage but also to the high NAR despite the high LAI. The rapid leaf expansion of Dasanbyeo at early growth stage seemed to be related in part to the profuse tillering capacity. HI was 0.53 in Dasanbyeo, 0,51 in Milyang 23, 0.41 in japonicas, and 0.35 in NPT line. Dasanbyeo was indebted for its higher HI to the relatively high grain filling ratio in spite of a much greater sink size than the other cultivars. Dasan had a greater source to sink ratio during grain ripening as measured by LAD/spikelet and dry matter production/spikelet which showed positove correlations with the grain ripening ratio. New plant type (NPT) line showed the lowest grain yield owing to the small sink size and the low grain filling ratio which seemed to have resulted from the abundant occurrence of weak-strength spikelets. The weak sink strength, in turn, seemed to have suppressed photosynthesis during the grain ripening stage.

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Environmental Mechanism on Seeding Stage and Ripening Period in Labour Saving-Direct Sowing Rice Culture (벼 성력재배를 위한 담수직파 파종시기와 등숙환경 분석)

  • 곽태순
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.6
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    • pp.541-549
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    • 1992
  • Lodging and rainfalls during ripening periods and ripening patterns were studied by the different maturing varieties and different seeding dates in labour saving-direct seeding cultivation of rice. Period of emergency in earlier seeding plot was longer than later seeding plot and standing seedlings per unit area and rate of standing seedlings were more and high by the later seeding date. Number of panicles per unit area was decreased by the later seeding dates. On the other hand, the rate of effective tillers was lowest at later seeding plot. Days from seeding to flowering were shortened by the later seeding dates due to the increase of daily growth temperature and reduce of day length. There was much rainfalls during ripening period of early maturing varietal group, however not much rainfalls during ripening period of medium and late maturing varietal groups. The degree of lodging at field was severer by the amount of rainfalls during ripening period. Earlier seeding plots and earlier maturing varietal groups. of used varieties were revealed heavier lodging compared to later seeding plots and later maturing varietal groups. Yield and yield related characteristics were high and good by the earlier seeding dates, especially ripening rate was clear.

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Influence of Ripening Stages on the Quality of Whole Crop Silage and Grain Silage of Fodder Rice

  • Maruyama, S.;Yokoyama, I.;Asai, H.;Sakaguchi, S.;Ohtani, T.;Yokota, H.;Kita, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.3
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    • pp.340-344
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    • 2005
  • In high-income Asian countries like Korea and Japan, per capita rice consumption has declined because of the change in consumer' favorite foods from rice to high-cost quality foods. This has forced farmers to reduce rice production. Although fodder rice could be another option to be cultured by farmers, available information concerning rice grain silage has been limited. In the present study, therefore, the difference in the quality of fodder rice silage prepared from either whole crop or grain at different ripening stages was compared. Various supplements were also added into whole crop and grain silages of fodder rice before ensiling, and thereafter, the palatability of prepared silages was determined by beef cattle. At ear emergence stage, the pH values for both grain and whole crop silages were approximately 4.5. In both grain and whole crop silages, the pH values were significantly increased by progressing ripening stages from milk-ripe stage to yellow-ripe stages, and the increase in pH value for grain silage was faster than that for whole crop silage. In the grain silage, the higher lactic acid (LA) content in grain silage seemed to be, the lower pH value was. Both in grain and whole crop silages, pH was significantly decreased by supplementation with LA bacteria. There were no significant differences in feed intake among any treatment groups.

Influences of Squid Ink Added to Low-Salted Squid Jeot-gal on Its Proteolytic Characteristics (오징어 먹즙 첨가가 저 식염 오징어 젓갈의 단백질분해 특성에 미치는 영향)

  • Oh, Sung-Cheon
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.2
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    • pp.348-355
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    • 2013
  • Squid ink was added to the salt fermented squid by 2% or 4% of concentration and ripened at $10^{\circ}C$ for 8 weeks and at $20^{\circ}C$ for 32days. The effects of the squid ink on the amino nitrogen and muscle protein of salt fermented squid were investigated. The results are as follows; As the salt concentration was decreased and the fermentation temperature raised, amino nitrogen in the salt fermented squid without addition of the squid ink was significantly increased to the latter stage of the ripening and hence fermentations were enhanced. From the change of the protein in the squid muscle in the experiments, dissolution of the myosin heavy chain took place conspicuously in the early stage of the ripening while actin was rarely changed which resulted in the strong resistance to protease. The amino nitrogen content in the salt fermented squid addition of the squid ink has increased to the latter part of the ripening but the range was smaller than no treatment groups. The protein in squid muscle, especially the myosin heavy chain was remarkably dissolved in the middle of the ripening whereas the squid ink added groups of high salt concentration and low temperature showed the tendency of slow proteolysis.

Reduction of quality of rice due to submergence during ripening stage and identification of its physiological cause

  • Lee, Hyeon-Seok;Back, Jung Seon;An, Sung Hyun;Jeong, Jae-Heok;Jeong, Han-Yong;Hwang, Woon-Ha;Yoon, Jong Tak;Lee, Gun-Hwi;Choi, Kyung-Jin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.227-227
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    • 2017
  • This study was conducted to analyze the reduction of quality and ripening rate of rice due to submergence during ripening stage and identify the physiological cause. Korean japonica type rice cultivars, Nampyeong was used in the experiment. The following 7, 14 day after heading, they were then moved into submergence treatment facility and we conducted the tests under three different submergence conditions - T1(clear water and overhead submergence), T2(Muddy water and exposure of Flag leaf end ), T3(Muddy water and overhead submergence), and the treatment lasted for 4days. The decrease of ripening rate was most severe in T3 treatment at 7days after heading, and the decrease of head rice ratio was most severe in T3 treatment at 14days after heading. Meanwhile the starch synthesis was inhibited, as the supply of assimilation products was inhibited in grain during the submergence treatment. And in stem, sucrose content was increased because the soluble carbohydrates accumulated before heading were decomposed. These changes may be due to the consumption of respiratory substrate in anaerobic conditions and the inhibition of the production of photosynthetic products by light interception. In order to see what kind of reaction occurs at the molecular level, we examined the degree of RNA expression in grain, stem and leaf. First, the expression of rna associated with starch synthesis in grain was decreased in all submergence treatment. and especially Ospul(DBE) was more decreased in 14days after heading treatment than 7days after heading treatment. This difference can explain the result that the decrease of head rice ratio was more severe at 14days after heading. And in stem, the expression of rna associated with the supply of assimilation products was decreased in submergence treatment. Finally in leaf, the expression of rna(ADH, ALDH) associated with anaerobic respiration was increased, while the expression of rna associated with photosynthesis was decreased. These results of physiological analysis can used to develop the cultivation technique and to offer the information for breeding the cultivars with tolerant characteristics to submergence stress during ripening stage in rice.

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Several Physico-chemical Characteristics of Kiwifruit (Actinidia chinensis Planch.) Depended on Cultivars and Ripening Stages (한국산 양다래(Actinidia chinensis Planch.)의 품종 및 숙도에 따른 이화학적 특성에 관하여)

  • Lee, Sei-Eun;Kim, Dong-Man;Kim, Kil-Hwan;Rhee, Chul
    • Korean Journal of Food Science and Technology
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    • v.21 no.6
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    • pp.863-868
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    • 1989
  • In comparison of contents of the components related to the quality of kiwifruit depended on the cultivars, the obtained results were as follows; Abbott showed the highest values in soluble solid, crude fat, firmness and specific gravity. Potassium, phosphorus, calcium and magnesium were estimated as the major minerals in kiwifruit and Bruno contained the highest amounts of potassium and magnesium. Bruno also had high concentrations of ascorbic, maleic, citric and fumaric acid when compared with the other cultivars. The changes in chemical components of kiwifruit (Hayward) by ripening stage were as follows; During ripening, the glucose and fructose contents were increased with decrease of sucrose content. Ascorbic, maleic, fumaric and succinic acid contents were considerably increased during ripening From the investigation of changes in protein pattern by electrophoresis! the new bands with about 17,000-23,50kd were shown during ripening.

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Study on Wine Quality of Domestic Grape Cultivar 'Cheonghyang' classified by Ripening Stage (국내 육성 품종 '청향' 포도의 숙도별 양조 적성 연구)

  • Lee, Hyo-Young;Jeon, Jin-A;Park, Young-Sik;Yi, Jae-Hyoung;Kwon, Hye-Jeong;Jeong, Seok-Tae
    • Journal of the East Asian Society of Dietary Life
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    • v.26 no.5
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    • pp.445-449
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    • 2016
  • In this study, Red pearl (4x) and MBA (2x) were crossed Gangwon-do Agricultural Research and Extension Services in 2000 to compare the brewing characteristics of Cheonghyang, a seedless kind of grape registered in 2009, by maturity. Fruit characteristics of Cheonghyang were examined after the first racking and 300 days after fermentation during aging in manufacturing wine based on maturity categories of being immature, mature, and overmature after harvest between August 25 and 27, 2014. The soluble solid matter content did not differ between the first racking following manufacturing wine and during ripening, and the alcohol content following the first racking was 12.9% for immature ones, 13.0% for mature ones, and 12.7% for overmature ones., The alcohol content decreased by appropriately 1.3% to 1.8% during ripening. The total acidity content was 0.9% for immature ones, 0.8% for mature ones, and 0.5% for overmature ones following the first racking and showed insignificant variations during ripening of 0.8%, 0.7%, and 0.5%, respectively, with wine made from mature ones showing proper acidity. The volatile acid content following the first racking during ripening, showing insignificant variation by maturity; however, it decreased after ripening. The sensory evaluation detected differences in acidity of wine between mature and overmature grapes. There was no difference in sour taste between immature and mature grapes; however, overmature ones had low levels of sour taste. Our results showed that mature wine had the best overall preference.

The Simple and Rough Screening Method of Phosphorus Deficient Tolerance Rice

  • Hwang, Woon-Ha;Kim, Dae-Wook;Jeong, Jae-Heok;Jeong, Han-Yong;Lee, Hyen-Seok;Choi, Kyung-Jin;Lee, Gun-Hwi;Lee, In-Jung;Oh, Sung-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.412-420
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    • 2015
  • Even though phosphorus (P) is essential element for plant growth and development, it is not enough for crop production in soil. To breed more P deficient tolerance rice, screening and selection in rice population is needed. We tried to develop more simple and rough screening method for breeding of P deficient tolerance rice. In P deficient condition, tiller number was dramatically decreased among yield components in rice. Though this result, we confirmed tiller number could be the best marker in screening of P deficient tolerance rice. 480 rice genetic resources were cultivated in rice bed tray filled with P deficient soil for four weeks and each dry weight was measured. Among them, the 55 kinds of genetic resource were selected then cultivated in paddy field with 3 fertilizer conditions. Plant dry weight and tiller number in ripening stage were shown significant difference according to P condition. Plant dry weight and tiller number in ripening stage was highly correlated especially in P deficient condition. Furthermore, the tiller number in ripening stage and plant dry weight in rough screening were shown high degree correlation. Though these results, we might expect measuring of plant dry weight after cultivation in rice bed tray filled with P deficient soil could be a simple and effective screening method in selection of P deficient tolerance rice.

The Effects of 1-Methylcyclopropene on the Quality of 'Ooishiwase' Plums (Prunus salicina L.) with Different Ripening Stage ('대석조생' 자두(Prunus salicina L.)의 숙기에 따른 1-Methylcyclopropene 처리효과)

  • Oh, Soh-Young;Lim, Byung-Seon;Lee, Jae-Wook;Lee, Ji-Hyun
    • Food Science and Preservation
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    • v.14 no.5
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    • pp.511-515
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    • 2007
  • 'Ooishiwase' plum (Prunus salicina L.) fruits were harvested at three pre-climacteric stages of ripeness (stages 1, 2, and 3) and treated with 1-methylcyclopropene (1-MCP, $1\;{\mu}L/L$) for 24 hours at $10^{\circ}C$ before storage to evaluate the effectiveness of 1-MCP in extending shelf-life at $10^{\circ}C$. Ethylene production and respiration rates were significantly lower after 1-MCP treatment compared to those of control fruit, throughout the entire storage period. Also 1-MCP delayed plum softening and color changes. However the chemical 1-MCP had no effect on fruit soluble solid content changes, the preservative 1-MCP is an effective tool for quality improvement in plums, and extension of shelf life of the fruit and plums can safely be harvested at stage 3 of ripening, at which time the most desirable organoleptic attributes have been developed.