• 식물병연구
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• 제20권3호
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• pp.189-195
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• 2014

Genetic engineering is being used to enhance disease resistance and nutritional value of crops including rice plant. Considering the fast-growing agricultural biotechnology and rapidly increasing global area of transgenic crops, the risk evaluation on environment is necessary. In this study, we surveyed the difference of disease occurrence between transgenic rice variety, Iksan526 transformed with peanut stilbene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong in the field. Moreover, the possibility of gene transfer from transgenic rice to bacterial and fungal pathogens was investigated. The results of this study indicated that there was no significant difference in the occurrence and severity of the diseases between Iksan526 and Dongjin or Nampyeong. In addition, the results suggested that rice pathogen, such as Xanthomonas oryzae pv. oryzae, Rhizoctonia solani and Magnaporthe grisea did not take up stilbene synthase and bar genes under natural conditions. Moreover the transformed DNA was not transferred to the pathogens even in repetitive contacts.

• 한국자원식물학회지
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• 제20권6호
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• pp.524-529
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• 2007

We carried out to study the function of ArgE in transgenic rice plants, which were confirmed by PCR analysis and hygromycin selection. Transgenic rice plants were with selectable marker gene(HPT) inserted in genome of the rice. Southern analysis with hpt probe confirmed by two restriction enzymes that copy numbers of the selectable gene was introduced into the plant genome. We displayed that the relationship between drought stress and ArgE gene with the overexpressing rice plants. From this result, we observed that the degree of leaves damage has no difference in control and transgenic lines. The total RNAs were extracted from 6 weeks-seedling in normal condition in order to examine their expression levels with ArgE-overexpressed transgenic rice. In particular, expression patterns of genes encoding enzymes involved in abiotic stress, including drought and salt stresses. OsGF14a and OsSalt were investigated by reverse transcription-PCR(RT-PCR). Expression levels of the OsSalt gene decreased significantly in transgenic rice plants compared to control plant. However, ion leakage measurement did not demonstrate any leaves damage change between control and ArgE transgenic plants exposure to mannitol treatment. These results suggest that expression of the ArgE is not involved in tolerance for drought stress in rice but may playa role of signaling networks for salt-induced genes.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.309-315
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• 2011

Rice grown in anaerobic waterlogged soil accumulates ammonium as a major source of nitrogen (N). We have compared the physiological symptoms of rice seedlings subjected to N-starvation stress with those receiving sufficient N, based on measurements of shoot/root length and weight and an analysis of protein expression patterns. N starvation marginally increased root growth but notably decreased shoot biomass. N uptake was reduced by >50% in the roots and shoots of N-starved seedlings. To better understand the mechanism of N starvation in rice, we performed a comparative proteome analysis of proteins isolated from rice leaves. Twenty-five differentially expressed proteins were analyzed by matrixassisted laser desorption/ionization time-of-flight (TOF) mass spectrometry and electron spray ionization quadrupole TOF. Functional analysis of the N-starvation response proteins suggested their involvement in protein synthesis and fate, metabolism, and defense. These results indicate that these proteins may play important roles in regulating the plant's complex adaptation responses for N use during N starvation. The proteins may be useful for further characterization of protein function in plant N nutrition.

• Molecules and Cells
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• 제38권8호
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• pp.685-696
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• 2015

Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.

• 한국토양비료학회지
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• 제4권1호
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• pp.1-11
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• 1971

1. 규산시용(珪酸施用)으로 수도(水稻)의 출수(出穗)가 수경(水耕)과 추낙답(秋落畓)에서 공(共)히 약(約) 일주일(一週日)이 빨렀고 수도(水稻)의 엽(葉)은 현저히 직립(直立)되였다. 2. 규산시용(珪酸施用)으로 수도(水稻)의 규산함량(珪酸含量)이 증가(增加)하며 추낙답(秋落畓)에서는 규산(珪酸)을 박비(迫肥)보다 기비(基肥)로 시용(施用)하는 것이 수도(水稻)의 규산함량(珪酸含量)을 증가(增加)시켰다. 규산시용(珪酸施用)으로 다른 양분(養分)의 함량(含量)은 감소(減少)되는 경향(傾向)이며 특(特)히 추락도(秋落稻)의 Fe 함량(含量)은 현저히 감소(減少)되었다. 3. 수도(水稻)의 규산함량(珪酸含量)과 엽도열병(葉稻熱病), 호마엽고병(胡麻葉枯病), 응애 및 멸구의 저항성(抵抗性)과는 부(負)의 상관(相關)이 있다. 4. 수도(水稻)에 대(對)한 규산(珪酸)의 증수효과(增收效果)는 수경(水耕)에서는 거의 없으나 추낙답(秋落畓)에서는 현저하다. 추낙답(秋落畓)에 대(對)한 규산(珪酸)의 증수효과(增收效果)는 pot 재배(栽培)보다 포장(圃場)에서 더 현저하다. 규산시용(珪酸施用)으로 추낙도(秋落稻)의 수당입수(穗當粒數)와 등숙률(登熟率)이 높아지고 규산(珪酸)의 비효(肥效)는 질소(窒素)와 가리(加里)의 균형시비(均衡施肥)로 증가(增加)한다. 5. 따라서 규산(珪酸)은 수도(水稻)에 대(對)한 필수원소(必須元素)라기보다 추낙답(秋落畓)과 같이 가급태규산함량(可給態珪酸含量)이 적은 토양(土壤)에서는 수도(水稻)의 건전생육(健全生育)을 위(爲)하여 농경학적(農耕學的) 의미(意味)에서 규산(珪酸)의 공급(供給)이 필요(必要)하다고 생각(生覺)된다.

• Plant Resources
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• 제5권2호
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• pp.104-108
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• 2002

In order to obtain basic information for selecting early maturing rice varieties which is suitable for early cropping before Alisma plantago in the southern part of Korea. Six rice varieties were grown from May to August in 2002 at Youngjeon Experiment Field, Sunchon and yield components and yield of plants were investingated. Early maturing rice cv. Grubyeo showed higher rough rice yield than any other varieties used in the experiment. It showed high yield components, such as culm length, panicle length, number of panicles per plant, number of spikelets per panicle and ratio of ripened grains. Therefore, it was concluded that Grubyeo was the most suitable variety with high yield for the cultivation before growing of Alisma plantago at the southern part of Korea. The heritability of culm length number of spikelets per panicle and rough rice yield were high and that of panicle length number of panicle per plant, ratio of ripened grain and 1,000 grain wt. of milled rice were low. According to the result of path coefficient analysis, characters highly correlated with rough rice yield showed large direct effects on rough rice yield.

• 한국토양비료학회지
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• 제34권3호
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• pp.173-177
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• 2001

This study was conducted to investigate a potential utilitization of multivariate statistical analysis(Factor analysis, Discrimination analysis) on interpretation of rice plant lodging reason. Rice plants were sampled in paddy around Taegu city at from 25 to 29 of September in 2000. Mineral nutrient content(phosphate, potassium) of rice plant were significantly higher at 99% level, Silicate content were lower at 95% level in lodged samples than in normal. Plant characteristics associate with lodging(Culm length, second and third internode length, bight of center gravity) were significantly longer in lodged rice plant than in non lodged. Result of Factor analysis were that first principle component were culm length, second(N2) and third internode length(N3), second principle component were Ca content, first internode length(N1) and N3/culm length, third principle component were center gravity length(G) and G/culm length, fourth were nitrogen, phosphate, and potassium content, fifth were N2/culm length, N2+N3/culm length, Sixth was silicate content of rice plant. Linear discriminant equation distinguished lodged rice plants with non lodged rice plants very well. Prediction value was 100%, most explainable variable were phosphate content, culm length and third length.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.130-136
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• 2011

Ions deficiency or excess remains one of the critical ground level environmental problems, affecting crop productivity. In this overview, we will discuss an increased application of proteomics technology in addressing this issue using rice (Oryza sativa L.) as a model crop plant. Proteomics analyses have revealed that rice proteome undergoes changes in the proteins composition and expression in response to several ionic stresses, including mineral nutrients (aluminum, nitrogen, and phosphorous) and heavy metals (arsenic, cadmium, and copper). Developed inventory of responsive proteins and their correlation with changes in physiological symptoms and parameters are a major step forward in: (i) better understanding the underlying mechanisms of ionic stresses-triggered responses in rice; (ii) comparative proteomics studies; and (iii) designing a novel strategy to improve crop plants.

• The Plant Pathology Journal
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• 제34권5호
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• pp.393-402
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• 2018

Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by Burkholderia glumae. The pathogen-induced symptoms include seedling rot, grain rot and leafsheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. However, it is still not fully elucidated the dynamics and permanence of the pathogen in the plant, from its entry until the development of disease symptoms in seedlings or panicles. In this work it was evaluated the infection of B. glumae rice plants, starting from inoculated seeds and substrates, and its subsequent monitoring after infection. Various organs of the plant during the vegetative stage and until the beginning of the reproductive stage, were evaluated. In both inoculation models, the bacteria was maintained in the plant as an endophyte between $1{\times}10^1$ and $1{\times}10^5cfu$ of B. $glumae.g^{-1}$ of plant throughout the vegetative stage. An increase of bacterial population towards initiation of the panicle was observed, and in the maturity of the grain, an endophyte population was identified in the flag leaf at $1{\times}10^6cfu$ of B. $glumae.g^{-1}$ fresh weight of rice plant, conducting towards the symptoms of bacterial panicle blight. The results found, suggest that B. glumae in rice plants developed from infected seeds or from the substrate, can colonize seedlings, establishing and maintaining a bacterial population over time, using rice plants as habitat to survive endophyticly until formation of bacterial panicle blight symptoms.

• Molecules and Cells
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• 제21권2호
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• pp.284-293
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• 2006

Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.