• Korean Journal of Weed Science
• /
• v.10 no.3
• /
• pp.171-178
• /
• 1990

This study was conducted to determine which of the different agronomic traits of rice (Oryza saliva L.) has greater weight in predicting thiobencarb (S-[(4-chlorophenyl) methyl]diethylcarbamothioate) tolerance. Differences in plant height, tiller number, culm length, panicle number, and spikelet number per panicle between tolerant and susceptible cultivars were greater at the higher herbicide rate. However, days to heading and percent filled spikelets were not affected by herbicide rate. At the higher thiobencarb rate, the coefficients for all characters except plant height, tiller number, and percent filled spikelets were significant. Spikelet number per panicle and panicle number which had positive significant coefficients at both concentrations are the most useful indicators of total filled spikelets.

• Journal of Life Science
• /
• v.14 no.2
• /
• pp.368-373
• /
• 2004

We have obtained fertile transgenic rice plants resistant to the broad spectrum herbicide Bast $a^{(R)}$ (active ingredient phosphinothricin, PPT) by PEG-mediated transformation procedure. The plasmid pCaMV35S::Bar was used to deliver the bar gene into embryogenic suspension culture-derived protoplasts of rice (Oryza sativa L.). Transformed plants were regenerated and selected on medium containing 15 mg/l of phosphinothricin. Stable integration and expression of the bar gene in transgenic rice plants was confirmed by Southern and Northern blot analysis. Transgenic $R_1$ plants were also confirmed by assays for phosphinothricin acetyltransferase (PAT) activity. The bar gene was expressed in the primary transgenic rice plants and in the next generation progeny, in which it showed a 3 : 1 Mendelian inheritance pattern. Transgenic $R_1$ and $R_2$ plants were resistant to the herbicide Bast $a^{(R)}$ when sprayed at rates used in field practice.ice.

• Korean Journal of Weed Science
• /
• v.16 no.3
• /
• pp.176-180
• /
• 1996

In order to estabilish a labour-saved and environmental protected paddy rice system in Southern Korea, new system, called no-tillage paddy system, was proposed and investigated from 1988 to 1996. Under the no-tillage paddy system, occurrence pattern, control value and regrowth of water foxtail each treatment(herbicides and application dates), and occurrence of volunteer rice plant in application dates of herbicide were investigated. The growth of water-foxtil was markedly increased from middle of May and no. of tillers and dry weight of water-foxtail increased up to 3rd crop year in no-tillage. Glyphosate application for control of water-foxtail was 20 days before transplanting. And when the soil under the no-tillage paddy system in May 1 and May 10 application of herbicide machine-transplanted with 8-day-old seedlings missing hills were increased compared to April 20 and early growth stages of machine transplanted of rice in April 20 applications of herbicide showed increased plant height, no. of tillers of rice, occurrence of volunteer rice plants from the shattered seeds were 1,600plants/10a and decreased in May 10 compared to April 20 and May 1.

• Weed & Turfgrass Science
• /
• v.3 no.4
• /
• pp.312-317
• /
• 2014

This study was conducted to obtain basis information for effective weed control by the fact-finding survey of herbicides use at farmer's level and distribution of herbicide resistant weeds in paddy field of Jeonbuk province, Korea. The distributions of major paddy field weeds and frequently used herbicide according to the survey were as follows. The dominant weeds in rice paddy field were Echinochloa crus-galli, Eleocharis kuroguwai, Scirpus juncoides, Monochoria vaginalis, etc.. Preferred herbicides widely used by farmers were ranked in the following order, early and middle treatment (48.0%), treatment before transplanting (36.7%) and foliar treatment (15.3%). The occurrence of herbicide-resistant weeds according to collected paddy soils was as follows. The occurring area of herbicide-resistant weeds was 24,413 ha, approximately 18.4% of rice cultivation area. Herbicide-resistant weeds were occurred in four species. The order of occurrence were Scirpus juncoides (39.0%) > Monochoria vaginalis (27.8%) > Echinochloa crus-galli (16.6%) and Cyperus difformis (16.6%). This information could be useful for estimation of future herbicide-resistant weed and establishment of herbicide-resistant weed control methods in Jeonbuk province, Korea.

• Korean Journal of Weed Science
• /
• v.32 no.1
• /
• pp.57-69
• /
• 2012

This study was conducted to find the effective management of main weeds including herbicide-resistant Echinochloa oryzicola, Scirpus juncoides and Monochoria vaginalis in direct seeded rice on puddled paddy surface. The dominant dominances in direct seeded rice on puddled paddy surface were increased for three years between 2009-2011. In weed distribution in 2011, the occurrence density of S. juncoides decreased greatly, but it of E. oryzicola increased especially as compared with it of 2009. Benzobicyclon, mesotrione and pyrimisulfan were effective to sulfonylurea(SU)-resistant S. juncoides and M. vaginalis at 5 and 12 days after seeding, however, the efficacy of bromobutide to sulfonylurea-resistant M. vaginalis was decreased slightly at 12 days after seeding. And carfentrazon and pyrazolate were ineffective to S. juncoides. In direct seeded rice on puddled paddy surface which ACCase and ALS inhibitor-resistant E. oryzicola was not occurred, benzobicyclon+penoxsulam+pyrazosulfuron-ethyl ET, bromobutide+imazosulfuron+metamifop GR, and flucetosulfuron+mesotrione+pretilachlor GR showed the control effect over 94% to main weeds included sulfonylurea-resistant S. juncoides and M. vaginalis. The E. oryzicola dominating in direct seeded rice on puddled paddy surface were not any affected to the survival by treatment with recommended doses of cyhalofop-butyl and penoxsulam tested. The ACCase and ALS inhibitor-resistant E. oryzicola was effectively controlled by mefenacet and fentrazamide up to the 2nd leaf stage. In direct seeded rice on puddled paddy surface domiating coincidentally by herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis, "one-shot herbicide" included with mefenacet, fentrazamide, penoxsulam and metamifop could't be used because of low control effect or rice injury. The sequential treatment of benzobicyclon+thiobencarb SE and bensulfuron+mefenacet+thiobencarb GR controlled effectively the herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis in direct seeded rice on puddled paddy surface. The days required by the 3rd leaf stage of herbicide-resistant E. oryzicola, S. juncoides and M. vaginalis in direct seeded rice on puddled paddy surface seeded in May 20 shortened by 4 days as compared with it of June 10.

• Korean Journal of Weed Science
• /
• v.7 no.1
• /
• pp.90-97
• /
• 1987

An attempt was done at the Yeongnam Crop Experiment Station in 1986-'87 to obtain herbicide tolerant variant through cell culture. Immatured rice grain was more rapidly and efficiently formed callus in dehulled rice culture method for both rice cultivar types, Tongil type (Indica/Japonica) and Japonica-type. However, Japonica-type cultivar was generally superior than Tongil-type Cultivar in callus formation. Expression rate of herbicide tolerant variant varied depending upon rice cultivar, plant species and herbicide properties. In case of Nagdongbyeo (Japonica) at the first subculture, 46.3% of total callus pieces appeared as herbicide tolerant variant in herbicide media of CGA142464 and followed by NC-311 (11.6%), Butachlor (7.5%), 2.4-D (2.1%), Quinclorac (0.89%), and Propanil (0.25%), in order. This degree of appearance of herbicide tolerant variants rapidly increased as passage of subculture was advanced. Herbicide tolerant callus hardly regenerated as normal plant even though large variations exhibited among culture media.

• Journal of Biosystems Engineering
• /
• v.31 no.1 s.114
• /
• pp.59-64
• /
• 2006

Application of herbicide for rice cropping is inevitable but notorious for its side effect of environmental pollution. Precision fanning will be one of important tools for the least input and sustainable fanning and could be achieved by implementation of the variable rating technology. If a device to detect weeds in rice field is available, herbicide could be applied only to the places where it is needed by the manner of the variable rating technology. The study was carried out to develop an algorithm of image processing to detect weeds in rice field using a machine vision system of multi-spectral digital images. A series of multi-spectral rice field picture of 560, 680 and 800 nm of center wavelengths were acquired from the 27th day to the 39th day after transplanting in the ineffective tillering stage of a rice growing period. A discrimination model to distinguish pixels of weeds from those of rice plant and weed image was developed. The model was proved as having accuracies of 83.6% and 58.9% for identifying the rice plant and the weed, respectively. The model was used in the algorithm to differentiate weed images from mingled images of rice plant and weed in a frame of rice field picture. The developed algorithm was tested with the acquired rice field pictures and resulted that 82.7%, 11.9% and 5.4% of weeds in the pictures were noted as the correctly detected, the undetected and the misclassified as rice, respectively, and 81.9% and 18.0% of rice plants in the pictures were marked as the correctly detected and the misclassified as weed, respectively.

• Weed & Turfgrass Science
• /
• v.3 no.4
• /
• pp.323-328
• /
• 2014

This study was conducted to find out the effects of aerial application by unmanned helicopter (AAUH) on controlling weeds under water-seeded rice cultivation. Foliar herbicide (bentazone sodium + fenoxaprop-P-ethyl) was applied with diluted 8-times (standard concentration pest control) as AAUH. Foliar herbicide treatment with standard and two times amount were little damage, but with more than three times amount showed great damage in rice growth. Six annual and two perennial weeds were major weeds occurred in the experimental paddy field. On foliar herbicide treatment 25 days after direct seeding, AAUH showed high control values against weeds (96.3% for annual weeds and 99.8% for perennial weeds). There was no significant difference in weed control values between AAUH and conventional application. There was no spray injury against rice plants with aerial application. In the experiment for good spray timing (15, 20 and 25 days after direct seeding) 15 days showed highest weed control values with 98.5% to annual weeds and 99.8% to perennial weeds and no spray injury.

• Korean Journal of Weed Science
• /
• v.7 no.2
• /
• pp.200-207
• /
• 1987

This study was conducted to select and develop herbicide resistant plant through tissue culture. Growth response of seedlings and callis of various rice varieties with Echinochloa species was assessed under the treatment of various rates of butachlor [N-(butoxy methyl)-2-chloro-2', 6'-diethyl acetamide] and simetryne [2,4-bis(ethyl amino)-6-methyl thio-1,3,5-triazine]. Further, succinate dehydrogenase activity was determined in herbicide treated callus to characterize different response of plants to herbicide. Rice variety like Sangpung showed relative resistance in both callus and seedling states against butachlor, indicating maintenance of resistance. However, in the simetryne treatment, the similar response was not observed in callus and seedling state, although there was a great different response among plant materials against simetryne. Rice variety which exhibited resistance in callus and seedling states showed low succinate dehydrogenase inhibition index. Succinate dehydrogenase inhibition index can be used as an important marker characters to differentiate varietal response of plant to herbicide. Rice plant was differentiated from butachlor and simetryne tolerant callus treated at $2.5{\times}10^{-5}$ M is growing under the growth chamber and can be used for resistant source.

• Korean Journal of Agricultural Science
• /
• v.40 no.4
• /
• pp.311-316
• /
• 2013

This study was conducted to evaluate the efficacy of two different formulations and application timings of benzobicyclon+penoxsulam+pyrzaosulfuron-ethyl (BPP) in rice field. BPP granule (GR) and tablet (TB) controlled more than 90% of four weed species 10 and 15 days after sowing in direct seeding when compared with untreated control. BPP TB was highly effective to control herbicide resistant Scirpus juncoides and Monochoria vaginalis both 10 and 15 days after transplanting (DAT). BPP GR controlled 94.0 and 96.5% of S. juncoides and M. vaginalis, respectively 10 DAT, but its efficacy decreased to 88.5 and 49.8% respectively 15 DAT. When compared with untreated control, no visual injuries were detected at 255 and 510 g a.i./ha of BPP. The yield of rice increased in both BPP GR and TB in transplanting and direct seeding rice cultivation when compared with untreated control. Based on these data, early application (10 DAT) of BPP GR and TB can be applied to provide effective weed control, especially in the field infested with herbicide resistant weed species.