• 제목/요약/키워드: rice genetics

검색결과 108건 처리시간 0.019초

KRDD: Korean Rice Ds-tagging Lines Database for Rice (Oryza sativa L. Dongjin)

  • Kim, Chang-Kug;Lee, Myung-Chul;Ahn, Byung-Ohg;Yun, Doh-Won;Yoon, Ung-Han;Suh, Seok-Cheol;Eun, Moo-Young;Hahn, Jang-Ho
    • Genomics & Informatics
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    • 제6권2호
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    • pp.64-67
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    • 2008
  • The Korean Rice Ds-tagging lines Database (KRDD) is designed to provide information about Ac/Ds insertion lines and activation tagging lines using japonica rice. This database has provided information on 18,158 Ds lines, which includes the ID, description, photo image, sequence information, and gene characteristics. The KRDD is visualized using a web-based graphical view, and anonymous users can query and browse the data using the search function. It has four major menus of web pages: (i) a Blast Search menu of a mutant line; Blast from rice Ds-tagging mutant lines; (ii) a primer design tool to identify genotypes of Ds insertion lines; (iii) a Phenotype menu for Ds lines, searching by identification name and phenotype characteristics; and (iv) a Management menu for Ds lines.

A Simple Purification Procedure of Biologically Active Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) Secreted in Rice Cell Suspension Culture

  • Sharma Niti;Park Seung Moon;Kwon Tae Ho;Kim Dae Hyuk;Yang Moon Sik
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제9권6호
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    • pp.423-427
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    • 2004
  • A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.

흑미의 자색종자과피 형질을 결정하는 대립유전자와 안토시아닌 생성의 상호관계 (Allelic Gene Interaction and Anthocyanin Biosynthesis of Purple Pericarp Trait for Yield Improvement in Black Rice)

  • 라만 모하마드 모미너;이경은;강상구
    • 생명과학회지
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    • 제26권6호
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    • pp.727-736
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    • 2016
  • 쌀은 인류가 가장 많이 먹고 있는 주식이다. 최근 백색미, 적색미, 갈색미, 녹색미 그리고 흑색미 등 다양한 품종들이 소비되고 있다. 고농도의 안토시아닌이 함유되어 있는 흑미는 영양가뿐만 아니라 암 발생 예방 효과가 있어 세계적으로 그 소비가 증가되고 있다. 유전학적으로 흑미는 cyanidin-3-O-glucoside (C3G)의 합성경로를 조절하는 두 개의 유전자 Pb와 Pp가 관여하는 Purple Pericarp (Prp) 유전형질에 의하여 종자과피의 색이 결정된다. 1921년 Nagai가 흑미의 유전현상을 일으키는 Prp 형질을 보고한 이 후 Prp 형질에 관여하는 유전자들과 그 기능 분석에 관한 많은 연구가 보고 되었으나 아직도 많은 부분이 밝혀지지 않았다. 그러나 안토시아닌의 함량이 높은 기능성 흑미의 소비는 증가하고 있는 반면 흑미는 일반 재배 벼에 비하여 상대적인 수확량이 매우 적어 농경제적인 문제가 있다. 본 논문에서는 흑미 종자가 성숙하는 과정에서 Prp 유전자의 유전현상의 특성과 안토시아닌 합성 경로 그리고 수확량에 관한 유전학적 관점에서 내용을 정리하였다.

Molecular Screening of Blast Resistance Genes in Rice using SSR Markers

  • Singh, A.K.;Singh, P.K.;Arya, Madhuri;Singh, N.K.;Singh, U.S.
    • The Plant Pathology Journal
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    • 제31권1호
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    • pp.12-24
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    • 2015
  • Rice Blast is the most devastating disease causing major yield losses in every year worldwide. It had been proved that using resistant rice varieties would be the most effective way to control this disease. Molecular screening and genetic diversities of major rice blast resistance genes were determined in 192 rice germplasm accessions using simple sequence repeat (SSR) markers. The genetic frequencies of the 10 major rice blast resistance genes varied from 19.79% to 54.69%. Seven accessions IC337593, IC346002, IC346004, IC346813, IC356117, IC356422 and IC383441 had maximum eight blast resistance gene, while FR13B, Hourakani, Kala Rata 1-24, Lemont, Brown Gora, IR87756-20-2-2-3, IC282418, IC356419, PKSLGR-1 and PKSLGR-39 had seven blast resistance genes. Twenty accessions possessed six genes, 36 accessions had five genes, 41 accessions had four genes, 38 accessions had three genes, 26 accessions had two genes, 13 accessions had single R gene and only one accession IC438644 does not possess any one blast resistant gene. Out of 192 accessions only 17 accessions harboured 7 to 8 blast resistance genes.

Variation in seedling growth inhibition due to Maleic Hydrazide treatment of rice(Oryza sativa) and ragi(Eleusine coracana) genotypes and its relationship with yield and adaptability

  • Das, Swarnalata;Sinha, Susil Kumar;Misra, Rama Chandra
    • Journal of Crop Science and Biotechnology
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    • 제11권3호
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    • pp.215-222
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    • 2008
  • Multilocation trials on 36 rice(Oryza sativa) genotypes of 3 different maturity groups were conducted at four different locations of Orissa for 3 years and 30 ragi(Eleusine coracana) genotypes of 2 different maturity groups were evaluated in three environmental conditions for 3 years. Grain yield data were subjected to stability analysis following linear regression model to estimate adaptability and stability parameters, i.e. b, and $S^2d$ Stability of performance of genotypes was also estimated by two other stability parameters viz., ecovalence W and AMMI stability value ASV. The rice and ragi genotypes of different duration groups showed wide variation in their mean yield, b, $S^2d$, W and ASV parameters. Seeds of the 36 rice and 30 ragi genotypes were treated with 500 and 100 ppm aqueous solution of maleic hydrazide(MH) for 24 hours, respectively to study MH-sensitivity. Sensitivity of genotypes to MH treatment was estimated in terms of seedling growth inhibition index(SGI). The rice and ragi genotypes showed wide differences in their MH-sensitivity in terms of SGI. Relationship of MH-sensitivity of genotypes with their yielding ability, adaptability and stability of performance was tested by contingency $x^2$ test. Low sensitivity of rice and ragi genotypes to MH in terms of SGI appeared to be good indicators of high yielding ability of genotypes. Also, low and high MH-sensitivity of genotypes would be a good indicator of better adaptability to rich and poor environments, respectively, in ragi but not in rice. Low MH-sensitivity of genotypes could be the good indicator of stability of yield performance in rice but not in ragi.

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Genetic diversity analysis of high yielding rice (Oryza sativa) varieties cultivated in Bangladesh

  • Epe, Isma Akter;Bir, Md. Shahidul Haque;Choudhury, Abul Kashem;Khatun, Asma;Aktar, Most Mohshina;Arefin, Md. Shamsul;Islam, Mohammed Aminul;Park, Kee Woong
    • 농업과학연구
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    • 제48권2호
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    • pp.283-297
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    • 2021
  • Investigation of genetic diversity and molecular characterization in high yielding rice varieties is important for their identification. The experiment was conducted during 2016 - 2017 to analyse the genetic diversity of fifteen high yielding rice varieties in Bangladesh by using random amplification of polymorphic DNA (RAPD) markers. Polymorphism was revealed in 12 RAPD primers out of 30, whereas no other reaction was detected on the remaining 18 primers. The 40 out of 45 bands (88.89%) polymorphics were produced by the primers and ranged from 50 to 100%. The maximum number of polymorphic bands was produced by the primer OPB-18 whereas the lowest number of polymorphic bands belonged to OPC-12. The genetic similarity coefficients were determined with the RAPD data, which ranged from 0.47 to 0.94. The unweighted paired group of arithmetic means (UPGMA) dendrogram presented the studied rice varieties into two major clusters. Moreover, the value of Nei's genetic diversity is 0.26 and the Shanon information index is 0.41. The study produced distinct positions, suggesting that the genotypes were different from each other. The results indicated that these markers could be efficient for comparing the genetic relationships, patterns of variation, and measurement of genetic distance among rice varieties. Considering all of these results, RAPD analysis is found to be an effective tool for estimating the genetic diversity of different rice varieties. The outcomes of this research may contribute to the germplasm data of rice accessions and a future breeding program of rice genotypes.