• 한국미생물·생명공학회지
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• 제30권4호
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• pp.410-414
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• 2002

아카시아꽃을 이용한 고부가가치의 생리기능성 전통주를 개발하기 위하여 먼저 아카시아 전통주의 알콜발효 조건을 검토하였다. 10%의 아카시아꽃을 함유한 덧밥에 누룩을 15% 첨가하고 Saccharomyces cerevisiae를 이용하여 제조한 주모를 첨가한 후 $25^{\circ}C$에서 20일간 발효시켰을 때 에탄올이 가장 많이 생성되었다. 또한, 기호도와 색상은 아카시아꽃 15% 첨가하여 제조한 전통주가 가장 좋았고 ACE 저해활성과 tyrosinase 저해활성은 각각 80.3%와 94.2% 이었고 전자공여능(23.4%)과 아질산염 제거활성(21.5%)도 대조구보다 높았다.

• 한국식생활문화학회지
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• 제33권2호
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• pp.133-141
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• 2018

This study investigated the quality characteristics and antioxidant activities of Makgeolli (a traditional Korean rice wine) made with Etteum bell flower root (0.5, 1, 1.5, and 2% with steamed rice) during fermentation. The pH values of Makgeolli with Etteum bell flower root powder decreased after 3 days of fermentation and then increased after 5 days of fermentation. Sugar content decreased after 3 days of fermentation. Color evaluation showed the L values of these drinks decreased during fermentation, whereas a and b values both increased. The alcohol content of Makgeolli increased after fermentation, reaching a maximum concentration of 7.90-8.07% by the end of fermentation. Total phenolic compound contents and DPPH radical scavenging activities increased as the ratio of Etteum bell flower root increased. Sensory scores of Makgeolli fermented with 1.5% Etteum bell flower root ware greater than those of Makgeolli prepared by other treatments. Therefore, Makgeolli added with 1.5% Etteum bell flower root added considered to be the most suitable for manufacturing.

• 한국작물학회지
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• 제44권2호
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• pp.171-175
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• 1999

Morphological alteration of floral organ development in rice affected by chilling stress was examined. Three varieties of rice were grown under natural conditions and subjected to 12$^{\circ}C$ for 3 or 6 days starting from the ineffective tillering stage, before heading stage and returned to natural condition. Headings were delayed by a 6 day chilling treatment. After heading the panicles were collected and examined for any possible alteration in floral organ development. It appears that there were some differences in sensitivity to chilling stress and degree of injury depending on treatment stages and variety. Chuchungbyeo was the most frequent in producing abnormal flowers among the three varieties examined. Meiosis stage was shown to be the most vulnerable to chilling stress in both Chuchungbyeo and Ilpumbyeo and young panicle differentiation stage was the frequent stage to alter flower development in response to chilling stress only in Chuchungbyeo. It was confirmed that abnormalities occurred in pollen due to chilling stress is a major factor leading to low yield, but to some extent the alterations in carpel development may playa certain role in determining a total yield in response to chilling stress at the reproduction stage in rice. There were abnormalities like extra stigmata, extra lemma, double ovary as well as abnormal anther formation in response to chilling stress. Further studies of the phenocopy observed in rice floral development may be useful for an understanding of the resistance against chilling injury during reproductive stages in rice.

• Molecules and Cells
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• 제26권5호
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• pp.474-480
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• 2008

OsMADS1 is a rice MADS box gene necessary for floral development. To identify the key cis-regulatory regions for its expression, we utilized transgenic rice plants expressing GUS fusion constructs. Histochemical analysis revealed that the 5.7-kb OsMADS1 intragenic sequences, encompassing exon 1, intron 1, and a part of exon 2, together with the 1.9-kb 5' upstream promoter region, are required for the GUS expression pattern that coincides with flower-preferential expression of OsMADS1. In contrast, the 5' upstream promoter sequence lacking this intragenic region caused ectopic expression of the reporter gene in both vegetative and reproductive tissues. Notably, incorporation of the intragenic region into the CaMV35S promoter directed the GUS expression pattern similar to that of the endogenous spatial expression of OsMADS1 in flowers. In addition, our transient gene expression assay revealed that the large first intron following the CaMV35S minimal promoter enhances flower-preferential expression of GUS. These results suggest that the OsMADS1 intragenic sequence, largely intron 1, contains a key regulatory region(s) essential for expression.

• The Korean Journal of Ecology
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• 제6권1호
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• pp.14-17
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• 1983

For the purpose of rice yield, 3 rice varieties S-235 (Sam-Pung), S-287(Tai-Baik), S-294(Nam-Pung), control experiment College of Agriculture, Seoul National University garden in 1982. Method of experiment are 4 treatment, 4 replication and randomized block design. Unhilled rice were increased S-235 14.8%, S-287 of 16.2%, S-294 of 15.7%. Each varieties were each sigmificant of 1%. And the best increased, Triacon 10 and $CaCl_2$ 15mM. It think that problems were the more study. Among factor of yield, increase is panice no. of hill and the same varieties. Only S-287 of panicle of hill is significant of 5% and also 1000 seed weight is not significant. The factor of increase yield is important of no. of flower. No. of flower increase is concerned Triacon, the mechanism will study.

• 한국식품영양학회지
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• 제24권4호
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• pp.746-752
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• 2011

반고형 형태의 이유식 제조에 가장 적합한 물성을 부여하는 점증제를 선정하기 위해 쌀가루, 변성전분 1 및 변성전분 2를 4%를 첨가하거나 0.3% 펙틴을 첨가한 이유식을 550 MPa 압력에서 3분 동안 초고압 처리하여 점도를 측정하고 관능평가를 실시한 결과, 쌀가루를 첨가한 바나나와 사과 이유식 모두 색, 흐름성 및 질감에서 가장 선호도가 높았을 뿐 아니라 시판 이유식과 가장 유사한 점도를 보여 반고형 상태의 이유식 제조에 쌀가루가 점증제로 적합하였다. 점증제로 선정된 쌀가루를 4%, 6% 및 8% 첨가하여 제조한 이유식을 450 MPa과 550 MPa의 압력 조건에서 1, 3, 5분 처리한 결과, 쌀가루 첨가 비율이 높아질수록 점도와 색도의 밝기는 증가하였고, pH와 당도에서 유의적인 차이는 없었다. 또한 초고압 처리 조건에서 압력의 변화와 처리시간의 증가에 따른 유의적인 차이를 보이지 않는다. 즉 초고압 처리가 이유식의 물성에 영향을 크게 미치지 않으므로 경제성 및 점증제의 호화 정도를 고려하여 이유식 제조를 위한 초고압 처리 조건은 550 MPa, 3 min이 가장 적당한 처리 조건으로 판단되었다.

• 한국육종학회지
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• 제43권1호
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• pp.1-13
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• 2011

Low temperature stress is one of the major negative factors affecting vegetative and reproductive growth of rice. To better understand responses of rice plants to low temperature we analyzed transcriptome expression patterns in glumous flower of cold-tolerant japonica rice variety, Stejaree45, and cold-susceptible variety, HR19621-AC6 at booting stage under cold water irrigation. A total of 2,411 probes were differentially expressed by low temperature in glumous flowers of the two varieties. Some important genes involved in hormone biosynthesis showed variety-specific regulation. Expression of GA20ox3 and GA2ox, among the genes involved in GA biosynthesis, was regulated differentially in the two varieties. Among the genes involved in IAA biosynthesis, YUCCA1 and TAA1:1 showed variety-specific regulation. Among the genes involved in cytokinin biosynthsis and signaling, expression of LOG, HK1 and HK3 was significantly down-regulated only in the cold-susceptible variety. Among the genes involved in ABA biosynthesis, NSY and AAO3 were down-regulated only in the cold-tolerant variety. In general, genes involved in GA, IAA and cytokinin biosynthesis responded to cold temperature in such a way that capacity of those bioactive hormones is maintained at relatively higher levels under cold temperature in the cold-tolerant variety, which can help minimize cold stress imposed to developing reproductive organs in the cold-tolerant variety.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1996년도 제10회 식물생명공학심포지움 고등식물 발생생물학의 최근 진보
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• pp.10-18
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• 1996

Recently, we have reported a rice MADS-box gene, OsMADS1, as a molecular factor triggering flower formation; this has been well studied in a heterologous system (Chung et al., 1994). In order to study whether the OsMADS1 homolog exists in other plant species, the OsMADS1 cDNA was used as a probe to screen a tobacco cDNA library, and a potential homolog, NtMADS3, was isolated. Sequence analysis revealed that the gene shares 56.1% identity in whole amino acids with OsMADS1. Like OsMADS1, the NtMADS3 gene starts to express at a very early stage of flower development, and the expression continues up to flower maturation. In the tobacco flower, the gene is expressed in whorl 2,3 and 4, corresponding to the petal, stamen, and carpel, respectively. Upon ectopic expression in the homologous system, NtMADS3 caused a trasition from inflorescence shoot meristem into floral meristem, reducing flowering time dramatically. These phenotypes strongly suggest the NtMADS3 gene is the OsMADS1 homolog of tobacco. Hybrids between the OsMADS1 and the NtMADS3 plants were also generated. The hybrids flowered even earlier than these two transgenic plants. The detailed studies are discussed here.

• 동아시아식생활학회지
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• 제24권3호
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• pp.283-290
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• 2014

The present study was performed to obtain data that could be used to popularize yakju(Korean traditional rice wine) by surveying how well rice wine goes with pairing foods. The survey was given to 302 men and women living in the Seoul and Gyunggi areas and work in the food service industry. The Jeon group, Gui group, Jeongol and Jjigae group, Pyeonyuk and Bossam group, and Muchim group were selected as menu items that go well with available rice wines. The survey aimed to identify foods that go well with four rice wines with different sensory characteristics. The survey results showed that both men and women replied that rice wines with Nuruk aroma and Nuruk taste go well with the Jeon group, rice wines with sour and flower aromas and common characteristics go well with the Muchim group, and rice wines with sweet and fruit tastes go well with the Muchim group. However, men and women had different opinions on rice wines having ginseng, soil, and herbal medicine aromas. Men replied that such rice wines go well with grilled meat, whereas women replied that these rice wines go well with Pyeonyuk and Bossan groups.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.21-35
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• 1999

We have previously isolated OsMADS4 gene that is a member of the class B MADS box genes from rice. In this study, another member of the class B MADS box genes was isolated from rice flower by the yeast two-hybrid screening method using OsMADS4 as bait. RNA blot analyses revealed that the clone, OsMADS16, was expressed in the second and third whorls, whereas the OsMADS4 transcripts were present in the second, third, and fourth whorls. These expression patterns of the OsMADS16 and OsMADS4 genes are very similar with those of AP3 and PI, the class B genes of Arabidopsis, respectively. In the yeast two-hybrid system, OsMADS4 interacted only with OsMADS16 among several rice MADS genes investigated, suggesting that OsMADS4 and OsMADS16 function as a heterodimer in specifying sepal and petal identities. We have also isolated OsMADS6 gene using OsMADS1 as a probe. Both are members of the AGL2 MADS family. Various MADS genes that encode for protein-protein interaction partners of the OsMADS6 protein were isolated by the yeast two-hybrid screening method. A majority of these genes belong to the AGL2 family. Sequence Homology, expression pattern, and ectopic expression phenotypes indicated that one of the interaction partners, OsMADS14, appears to be homologous to API, the class A MADS gene of Arabidopsis.