• 한국토양비료학회지
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• 제14권2호
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• pp.76-82
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• 1981

수도체의 영업조건(營業條件) 특히 생육시기별(生育時期別) 규산(珪酸)의 공급조건(供給條件)과 도열병 발병(發病)과의 실계를 밝히고자 밀양(密陽)23호와 낙동(洛東)벼 (밀양(密陽)15호)를 공시(供試)하여 질소수준(窒素水準)을 달리하고 생육시기별(生育時期別) 규산(珪酸)의 공급조건(供給條件)을 달리하여 수경시험(水耕試驗)을 실시(實施)한 결과(結果)를 요약(要約)하연 다음과 같다. 1. 규산(珪酸) 시용(施用)으로 도열병 이병률(罹病率)은 현저(顯著)히 감소(減少)하였으며 이는 주(主)로 수도분중 규산함량(珪酸含量)의 증가와 질소(窒素) 함량(含量)의 감소(減少)에 기인(起因)되었다. 2. 유수형성기(幼穗形成期) 이후(以後)의 규산(珪酸) 시용(施用)으로 목도열병 이병수률(罹病穗率)은 현저(顯著)히 감소(減少)하여 규산(珪酸) 추비효과(追肥效果)의 가능성(可能性)을 보였다. 3. 규산(珪酸)의 도열병 억제효과(抑制效果)는 잎도열병보다 목도열병에서 뚜렷했다.

• The Plant Pathology Journal
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• 제25권4호
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• pp.429-432
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• 2009

An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.

• The Plant Pathology Journal
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• 제32권4호
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• pp.357-362
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• 2016

ALD1 (ABERRANT GROWTH AND DEATH2 [AGD2]-LIKE DEFENSE1) is one of the key defense regulators in Arabidopsis thaliana and Nicotiana benthamiana. In these model plants, ALD1 is responsible for triggering basal defense response and systemic resistance against bacterial infection. As well ALD1 is involved in the production of pipecolic acid and an unidentified compound(s) for systemic resistance and priming syndrome, respectively. These previous studies proposed that ALD1 is a potential candidate for developing genetically modified (GM) plants that may be resistant to pathogen infection. Here we introduce a role of ALD1-LIKE gene of Oryza sativa, named as OsALD1, during plant immunity. OsALD1 mRNA was strongly transcribed in the infected leaves of rice plants by Magnaporthe oryzae, the rice blast fungus. OsALD1 proteins predominantly localized at the chloroplast in the plant cells. GM rice plants over-expressing OsALD1 were resistant to the fungal infection. The stable expression of OsALD1 also triggered strong mRNA expression of PATHOGENESIS-RELATED PROTEIN1 genes in the leaves of rice plants during infection. Taken together, we conclude that OsALD1 plays a role in disease resistance response of rice against the infection with rice blast fungus.

• 한국유기농업학회지
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• 제13권3호
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• pp.291-300
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• 2005

우리나라 벼 유기농업 실천농가의 가장 큰 애로사항은 알려져 있는 병해 및 잡초 제어기술을 정립하기 위하여 강화 등 5개 지역의 벼 유기농재배지와 관행재배지에서 병해충의 발생 실태를 조사한 결과는 다음과 같다. 1. 병해 발생조사결과 도열병은 관행재배지보다 오리농법 재배지에서 발병이 많았고 품종별로는 추청벼 보다 일품벼에서 발병이 많았으며, 잎집무의마름병은 오리농법과 쌀겨농법재배지에서 발병이 많았고 품종별로는 추청벼 보다 일품벼에서 발병이 많았으며, 문고병도 관행재배지보다 오리농법과 쌀겨농법에서 발병이 많은 경향이었다. 2. 친환경 자재의 벼 종자소독 효과시험 결과 현미식초 1,000배액이 키다리병 방제에 효과가 있었으며 발아율도 양호하였고, 시중에 종자소독용으로 유통되고 있는 친환경자재의 도열병에 대한 예방 및 치료효과는 미미하였다. 3. 헤어리벳치 재배구의 헤어리벳치 건물 중이 이앙시기에 추천량의 1/40에 불과하여 헤어리벳치 재배에 의한 토양물리성의 변화는 없었다. 4. 헤어리베치 잎과 뿌리의 추출물질은 상추종자의 발아 억제효과를 보였다.

• 한국환경농학회지
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• 제38권3호
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• pp.185-196
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• 2019

BACKGROUND: This study investigated the effects of rice genetically modified to be resistant against rice blast and rice bacterial blight on the soil microbial community. A comparative analysis of the effects of rice genetically modified rice choline kinase (OsCK1) gene for disease resistance (GM rice) and the Nakdong parental cultivar (non-GM rice) on the soil microbial community at each stage was conducted using rhizosphere soil of the OsCK1 and Nakdong rice. METHODS AND RESULTS: The soil chemistry at each growth stage and the bacterial and fungal population densities were analyzed. Soil DNA was extracted from the samples, and the microbial community structures of the two soils were analyzed by pyrosequencing. No significant differences were observed in the soil chemistry and microbial population density between the two soils. The taxonomic analysis showed that Chloroflexi, Proteobacteria, Firmicutes, Actinobacteria, and Acidobacteria were present in all soils as the major phyla. Although the source tracking analysis per phylogenetic rank revealed that there were differences in the bacteria between the GM and non-GM soil as well as among the cultivation stages, the GM and non-GM soil were grouped according to the growth stages in the UPGMA dendrogram analysis. CONCLUSION: The difference in bacterial distributions between Nakdong and OsCK1 rice soils at each phylogenetic level detected in microbial community analysis by pyrosequencing may be due to the genetic modification done on GM rice or due to heterogeneity of the soil environment. In order to clarify this, it is necessary to analyze changes in root exudates along with the expression of transgene. A more detailed study involving additional multilateral soil analyses is required.

• 농약과학회지
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• 제19권4호
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• pp.394-398
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• 2015

본 연구는 잎집무늬마름병에 대한 약제방제수준을 설정하기 위하여 오대벼를 공시하여 시험을 수행하였다. 잎집무늬마름병 발병정도에 따라 수량에 미치는 관계를 분석하였으며, 수량 구성 요소인 주당 이삭수, 이삭당 립수, 등숙률, 천립중과의 관계를 분석하였다. 그 결과 발병주율에 대한 수량과의 관계는 r = -0.93, 이삭당 립수 r = -0.66, 등숙율은 r = -0.77로 고도의 유의성 있는 부의 상관이 있었으나, 주당 이삭수와 천립중은 유의한 상관이 인정되지 않았다. 따라서 본시험에서는 발병과 상관이 높은 수량에 대하여 경제적 방제수준은 발병주율 7.8%로 설정할 수 있었다.

• 식물병연구
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• 제7권3호
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• pp.150-154
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• 2001

세균성벼알마름병의 발생 예측 모델을 만들기 위하여 출수기의 기상 환경과 병 발생과의 관계를 자료분석(1992~2000)과 온실 및 포장시험을 통해 조사하였다. 세균성벼알마름병의 발생이 많은 해는 '94, '95, '98, 2000년이었고, 중만생종 출수기인 8월 상.중순에 고온이 지속되면서 연속강우가 많았다. 반면 병 발생이 적었던 '93년은 강우량과 강우일수는 많았으나 저온이었고, '97년에는 온도는 높았으나 강우가 적은 경향이었다. 출수기에 접종 후 습실처리 하였을 때(24시간 이상) $10^2$cfu/ml에서부터 발병하기 시작하여 접종농도가 높을수록 발병도가 높아져 $10^{8}$ cfu/ml에서는 86.1%의 발병도를 보였다. 습실처리를 하지 않은 경우에는 $10^{8}$ cfu/ml에서만 12.5% 발병하였다. 또한 발병도가 증가함에 따라 천립중과 등숙률이 현저히 감소하였다.

• The Plant Pathology Journal
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• 제32권1호
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• pp.8-15
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• 2016

To identify a novel biopesticide controlling rice blast disease caused by Magnaporthe oryzae, 700 plant extracts were evaluated for their inhibitory effects on mycelial growth of M. oryzae. The L. foenum-graecum Herba extract showed the lowest inhibition concentration ($IC_{50}$) of $39.28{\mu}g/ml$, which is lower than the $IC_{50}$ of blasticidin S ($63.06{\mu}g/ml$), a conventional fungicide for rice blast disease. When treatments were combined, the $IC_{50}$ of blasticidin S was dramatically reduced to $10.67{\mu}g/ml$. Since ABC transporter genes are involved in fungicide resistance of many organisms, we performed RT-PCR to investigate the transcriptional changes of 40 ABC transporter family genes of M. oryzae treated with the plant extract, blasticidin S, and tetrandrine, a recognized ABC transporter inhibitor. Four ABC transporter genes were prominently activated by blasticidin S treatment, but were suppressed by combinational treatment of blasticidin S with the plant extract, or with tetrandrine that didn't show cellular toxicity by itself in this study. Mycelial death was detected via confocal microscopy at 24 h after plant extract treatment. Finally, subsequent rice field study revealed that the plant extract had high control efficacy of 63.3% and should be considered a biopesticide for rice blast disease. These results showed that extract of L. foenum graecum Herba suppresses M. oryzae ABC transporter genes inducing mycelial death and therefore may be a potent novel biopesticide.

• The Plant Pathology Journal
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• 제31권4호
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• pp.350-362
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• 2015

A disease forecast model for bacterial grain rot (BGR) of rice, which is caused by Burkholderia glumae, was developed in this study. The model, which was named 'BGRcast', determined daily conduciveness of weather conditions to epidemic development of BGR and forecasted risk of BGR development. All data that were used to develop and validate the BGRcast model were collected from field observations on disease incidence at Naju, Korea during 1998-2004 and 2010. In this study, we have proposed the environmental conduciveness as a measure of conduciveness of weather conditions for population growth of B. glumae and panicle infection in the field. The BGRcast calculated daily environmental conduciveness, $C_i$, based on daily minimum temperature and daily average relative humidity. With regard to the developmental stages of rice plants, the epidemic development of BGR was divided into three phases, i.e., lag, inoculum build-up and infection phases. Daily average of $C_i$ was calculated for the inoculum build-up phase ($C_{inf}$) and the infection phase ($C_{inc}$). The $C_{inc}$ and $C_{inf}$ were considered environmental conduciveness for the periods of inoculum build-up in association with rice plants and panicle infection during the heading stage, respectively. The BGRcast model was able to forecast actual occurrence of BGR at the probability of 71.4% and its false alarm ratio was 47.6%. With the thresholds of $C_{inc}=0.3$ and $C_{inf}=0.5$, the model was able to provide advisories that could be used to make decisions on whether to spray bactericide at the preand post-heading stage.

• Plant Resources
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• 제7권1호
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• pp.36-38
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• 2004

Bacterial grain rot of rice caused by Burkholderia glumae was examined between weather condition and disease incidence. From 1998 to 2000, average disease incidence was 3.0 ％ without difference in survey regions. However, it was related closely to amount of rainfall that disease incidence higher in 1998 and 2000 to 3.0 ％ and 3.6 ％ respectively than 2.3 in 1999 relatively small volum of rainfall season.