• International Journal of Stem Cells
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• v.16 no.1
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• pp.93-107
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• 2023

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.

• Journal of Korea Water Resources Association
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• v.33 no.4
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• pp.407-417
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• 2000

To estimate the longitudinal dispersion coefficient at the downstream of Jungrang-River, the undistorted 1/20 scale hydraulic model was used in this study. Experiments were conducted for dry season discharge, and Rhodamine B was used as a tracer. The relationship curve between concentration and conductivity of Rhodamine B was otained by laboratory test, and the conductivity which was measured in hydraulic model was converted to concentration using this curve. The longitudinal dispersion coefficient was calculated using the relationship between the peak concentration and the time to peak concentration. The results of this study were compared with the calculated values by the empirical equations for the longitudinal dispersion coefficient and with the field data. The results of comparison show that Parker's equation underestimates, and Liu'g equation and Iwasa and Aya's one overestimate, and McQuivey and Keefer's equations, Fischer's one, Magazine's one, and Seo and Cheong's one predict relatively well. The measured data sets were relatively close to the observed ones in natural river. The longitudinal dispersion coefficient at the downstream of Jungrang-River was estimated $10\textrm{m}^2/s$.

• Journal of Environmental Science International
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• v.14 no.12
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• pp.1203-1209
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• 2005

Basic dyes, Rhodamine 6G(R6G), Rhodamine B(RB), and Methylene Blue(MB), dissolved in water were used to investigate single-component adsorption affinity to the pearl layer fractionated according to the size. Unfractionated pearl layers were also used as adsorbents for the R6G and RB. The Langmuir and the Redlich-Peterson(RP) models were used to fit the adsorption data, and the goodness of fit was examined by using determination coefficient($R^2$) and standard deviation(SSE). The 3-parameter RP model was found to be better in describing the dye adsorption data than the 2 parameter Langmuir model, as can be expected from the number of parameters involved in the model. The adsorption affinity to the fractionated pearl layer was higher than that to the unfractionated layer The affinity order to the fractionated Conchiolin layer was found to be R6G > MB > RB. Furthermore, the dye adsorption capacity of the various types of pearl layer was found to be in the order, the fractionated pearl > powdered pearl > unfractionated pearl, exhibiting different adsorption isotherms according to the types of layer used in the study.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2018.10a
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• pp.165-165
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• 2018

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2459-2464
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• 2007

In present study, a temperature field of specimens which was coated with fluorescence dye such as Rhodamine-B(Rh-B) has been measured, based on the fluorescence intensity. Silica(SiO2) nano porous structure with 1um thickness was constructed on a cover glass, and fluorescence dye was digested into these porous thin films. To optimize manufacturing coating process, various solvents, Rh-B concentration, and other chemical materials were applied to fabricate the specimen and all specimens were measured on the various temperature conditions. For the measurement, a 14 bit cooled CCD camera with 1600 by 1200 spatial resolution is equipped with epifluorescence microscope to obtain only fluorescence intensity from 1.2 mm by 0.9 mm field of view of the illuminated coated specimen.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.3
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• pp.365-370
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• 2004

Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereo Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K${\times}$2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent flow around Rushton turbine were identified by the calculation of synchronized data of the velocity field and concentration field.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.267-270
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• 2003

수용성 양이온 안료인 RhB를 대상으로 분말 $TiO_2$와 고정화 $TiO_2$를 이용하여 색도제거실험을 수행한 결과 다음과 같은 결론을 얻었다. 1) 분말 $TiO_2$의 최적 투입량은 0.2 g/L였으며, 대부분 광촉매 반응에 의하여 제거되었다. 고정화 $TiO_2$의 최적 투입량은 210.7 g/L이었고, 광촉매와 흡착에 의해 제거되었다. Langmuir - Hinshelwood 식으로 표현되지 못하였다. 2) 고정화 $TiO_2$를 이용하여 Rhodamine B를 제거할 경우 광촉매 분리 문제는 해결할 수 있으며, 광 투과율을 증가시킬 수 있으나 같은 제거율을 얻기 위해 투입량이 분말에 비해 매우 높은 것이 문제점으로 나타났다.

• Journal of Environmental Science International
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• v.7 no.3
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• pp.263-268
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• 1998

Decolorization of congo red, rhodamlne B was investigated by the white rot fungus Irpex lacteus welch has biodegrading capability of various recalcitrants. White rot fungus Irpex lacteus is Immobilized by PVA-freezing method. An Immobilized Irpex lacteus decolorizes 91% of congo red In 8 days under culture with glucose 2%(Initial cone.). It also showed 70% of decolorization at 3 days In the state of putting MnSO4 1mM. But, rhodauune B has no significant differences about decolorization among different mixture ratio of Irprx lacteus with PVA, concentration of carbon, nitrogen and manganese sulfate.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.4
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• pp.504-510
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• 2003

Simultaneous measurements of turbulent velocity and concentration field in a stirred mixer tank are carried out by using PIV/LIF technique. Instantaneous velocity fields are measured with a 1K$\times$1K CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.5
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• pp.587-593
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• 2004

In this paper, flow characteristics of plasma flow in a micro-tube were investigated experimentally using micro particle image velocimetry(micro-PIV). For comparison, the experiments were repeated for deionized(DI) wale. instead of plasma. Both velocity profiles of plasma and do-ionized water are well agreed with the theoretical velocity distribution of newtonian fluid. We also carried out generating plasma-in-oil droplet formation at a Y-junction microchannel. In order to clarify the hydrodynamic aspects involved in plasma droplet formation, Rhodamine-B were mixed with plasma only for visualization of plasma droplet. With oil as the continuous phase and plasma as the dispersed phase, plasma droplet can be generated in a continuous phase flow at a Y-junction. For given experimental parameters, regular-sized droplets are reproducibly formed at a uniform flow conditions.